Fibroblast growth factor receptors(FGFRs)play an important role in the regulation of cell proliferation,migration and differentiation,while the juxtamembrane domain(JMD)of FGFRs is the key in mediating these transmemb...Fibroblast growth factor receptors(FGFRs)play an important role in the regulation of cell proliferation,migration and differentiation,while the juxtamembrane domain(JMD)of FGFRs is the key in mediating these transmembrane signal transduction processes.Here,we expressed and purified the JMD(398K-470R)of FGFR1 with the presence of transmembrane domain(377I-397Y).The results from nuclear magnetic resonance(NMR)chemical shift analysis demonstrate that the main structure of JMD is disordered.Yet,the N-terminus of JMD was observed to form a short a-helix upon introducing negatively charged lipid 1,2-dioleoyl-sn-glycero-3-phospho-L-serine(DOPS)into its membrane mimic bicelles.Moreover,the N-terminus of JMD interacts with FRS2a,which is a substrate 2a of FGFR.Hence,we propose a model that FGFR1-JMD may interact with FRS2a and negatively charged lipids competitively.Our study provides a new understanding on the role of the JMD of FGFRs.展开更多
基金Special Foundation of President of the Chinese Academy of Sciences(Grant No.,YZJJ2021QN33,YZJJ2020QN27)Collaborative Innovation Program of Hefei Science Center,CAS(2020HSC-CIP008).
文摘Fibroblast growth factor receptors(FGFRs)play an important role in the regulation of cell proliferation,migration and differentiation,while the juxtamembrane domain(JMD)of FGFRs is the key in mediating these transmembrane signal transduction processes.Here,we expressed and purified the JMD(398K-470R)of FGFR1 with the presence of transmembrane domain(377I-397Y).The results from nuclear magnetic resonance(NMR)chemical shift analysis demonstrate that the main structure of JMD is disordered.Yet,the N-terminus of JMD was observed to form a short a-helix upon introducing negatively charged lipid 1,2-dioleoyl-sn-glycero-3-phospho-L-serine(DOPS)into its membrane mimic bicelles.Moreover,the N-terminus of JMD interacts with FRS2a,which is a substrate 2a of FGFR.Hence,we propose a model that FGFR1-JMD may interact with FRS2a and negatively charged lipids competitively.Our study provides a new understanding on the role of the JMD of FGFRs.
