Direct-sowing establishment method has great significance in improving winter oilseed rape(Brassica napus L.) production and guaranteeing edible oil security in China. However, nutrient responses on direct sown wint...Direct-sowing establishment method has great significance in improving winter oilseed rape(Brassica napus L.) production and guaranteeing edible oil security in China. However, nutrient responses on direct sown winter oilseed rape(DOR) performance and population development dynamic are still not well understood. Therefore, five on-farm experiments were conducted in the reaches of the Yangtze River(RYR) to determine the effects of nitrogen(N), phosphorus(P), and potassium(K) deficiencies on population density, dry matter production, nutrient uptake, seed yield, and yield components of DOR plants. Four fertilization treatments included the balanced NPK application treatment(NPK, 180 kg N, 39.3 kg P, 100 kg K, and 1.8 kg borax ha–1) and three nutrient deficiency treatments based on the NPK treatment, i.e., –N, –P, and –K. The results indicated that DOR population density declined gradually throughout the growing season, especially at over-wintering and pod-development stages. Nutrient deficiency decreased nutrient concentration in DOR plants, limited dry matter production and nutrient uptake, and thereby exacerbated density reduction during plants growth. The poor individual growth and reduced population density together decreased seed yield in the nutrient deficiency treatment. Averaged across all the experiments, seed yield reduced 61% by N deficiency, 38.3% by P deficiency, and 14.4% by K deficiency. The negative effects of nutrient deficiency on DOR performances followed the order of –N–P–K, and the effects were various among different nutrient deficiencies. Although N deficiency improved DOR emergence, but it seriously limited dry matter production and nutrient uptake, which in turn led to substantial plants death and therefore resulted in a very low harvested density. The P deficiency significantly reduced initial density, limited plants growth, and exacerbated density reduction. The K deficiency mainly decreased individual growth and yield, but did not affect density dynamic. Our results highlighted the importance of balanced NPK application in DOR production, suggesting that management strategy of these nutrients should be comprehensively considered with an aim to build an appropriate population structure with balanced plant density and individual growth.展开更多
The proper use of organic and inorganic nutrient sources is important to sustain high levels of crop production, while maintaining or enhancing soil and environmental quality. A 4-year (2009 to 2012) field experiment ...The proper use of organic and inorganic nutrient sources is important to sustain high levels of crop production, while maintaining or enhancing soil and environmental quality. A 4-year (2009 to 2012) field experiment was established in spring 2009 on a Gray Luvisol (Typic Haplocryalf) loam soil at Star City, Saskatchewan, Canada, to determine the effectiveness of organic/biological (compost, wood ash [fine and granular], alfalfa pellets, distiller grain, thin stillage, glycerol, fish food additive, Penicillium bilaiae), inorganic/mineral (granular-gypsum, rapid release elemental S [RRES], rock phosphate [granular and fine]) and chemical/synthetic (granular-ammonium nitrate, triple super phosphate and potassium sulphate) nutrient sources (amendments/chemicals) in improving seed yield, straw yield, seed quality and nutrient uptake (N, P, K and S) in seed + straw of canola. Combined application of N, P and S chemical fertilizers (NPS) produced considerably greater seed yield, straw yield and nutrient uptake of canola compared to the unamended control in all four years. In treatments receiving only organic amendments, thin stillage produced the greatest seed yield, straw yield and nutrient uptake in all years, and it was similar to the NPS balanced fertilizer treatment, while fish food additive and distiller grain dry of wheat in 2009, 2011 and 2012, distiller grain dry of corn in 2009 and 2012, and compost and alfalfa pellets in 2011 and 2012 produced significantly greater seed yield, straw yield and nutrient uptake, when compared to the control. In treatments where chemical fertilizers were also applied, in addition to organic amendments, ap- plication of N fertilizer increased seed yield, straw yield and nutrient uptake substantially when combined with wood ash fine in 2009, 2010, 2011 and 2012, wood ash granular in 2009, 2011 and 2012, and glycerol in 2009 and 2012 (moderate increase in 2012). In the chemical fertilizer treatments, there was a reduction in seed yield, straw yield and nutrient uptake of canola when only N fertilizer was applied compared to the control (significant in 2010 and 2011). Application of P along with N (NP) increased seed yield, straw yield and nutrient uptake of canola compared to N alone treatment, but was less than the NPS treatment in all years. Application of S along with N (NS) increased seed yield, straw yield and nutrient uptake of canola further compared to the NP treatment, but it was still lower than the NPS treatment in 2010 and 2011. In treatments receiving inorganic/mineral amendments in addition to chemical fertilizers, application of N + P fertilizers substantially increased seed yield, straw yield and nutrient uptake in treatments receiving gypsum and RRES in 2009, 2010, 2011 and 2012. This suggests the potential of gypsum and RRES in preventing S deficiency in organic crops when grown on S-deficient soils, provided other nutrients are not limiting in the soil for crop growth. Seed yield, straw yield and nutrient uptake with application of N and S fertilizers in combination with rock phosphate and/or Penicillium bilaiae were similar to N + S treatment in most cases, except in 2011 when application of finely-ground or powder rock phosphate in a combination with N + S produced significantly greater yield and nutrient uptake than N + S with granular rock phosphate. This suggests little contribution of rock phosphate and/or Penicillium bilaiae in improving yield and nutrient uptake of canola, and improves the performance of fine rock phosphate only evident in the third growing season in 2011, after three consecutive applications, but not in 2012. In conclusion, some organic amendments showed potential for improvement in organic crop production, and in some other cases highest yield and nutrient uptake were展开更多
Cotton(Gossypium spp.) is the leading fiber crop,and an important source of the important edible oil and protein meals in the world.Complex genetics and strong environmental effects hinder
Two field studies were carried out using Solanum macrocarpon and Solanum scabrum to investigate the effects of planting methods and seed density required for optimum biomass yield and nutrient content in southwest Nig...Two field studies were carried out using Solanum macrocarpon and Solanum scabrum to investigate the effects of planting methods and seed density required for optimum biomass yield and nutrient content in southwest Nigeria. Two planting methods (drilling and broadcasting), and two seed densities (4 and 8 table spoon full) were investigated as a 2×2×3 factorial experiment in randomized complete block design and replicated three times. Four seed spoons gave 25.78 kg seeds ha-1 for S. macrocarpon and 20.67 kg·seeds·ha-1 for S. scabrum. Significant highest S. macrocarpon shoots (2.75 kg·m-2) and Ca, K, Fe and Zn contents and S. scabrum (2.95 kg·m-2) and Zn content, were obtained with drilling and four spoons of seeds compared with broadcasting and 8 spoons of seeds. This treatment gave average values for N, P, K, Mg, Ca, Na, Fe, Zn, Mn, Cu, Ca/P and Na/K ratio were 2.79%, 0.03%, 3.37%, 1.64%, 0.02%, 300 mg·kg-1, 100 mg·kg-1, 300 mg·kg-1, 8 mg·kg-1, 1.22 and 0.008 for S. macrocarpon and 2.05%, 0.27%, 5.81%, 3.27%, 2.91%, 0.09%, 280 mg·kg-1, 52.99 mg·kg-1, 359.35 mg·kg-1 and 22.42 mg·kg-1, 11.63 and 0.016 for S. scabrum. It was concluded that planting in drilling made weeding, fertilizer application, irrigation and harvesting more effective rather than by broadcasting, and four spoons of seeds per 9 m-2 produced deep green and broader leaves and balanced nutrient contents than eight spoons of seeds.展开更多
Differences in soil physical, chemical and biological properties between paddy–upland and continuous upland rotations will influence nutrient relations and crop growth. With the aim of estimating rapeseed yield perfo...Differences in soil physical, chemical and biological properties between paddy–upland and continuous upland rotations will influence nutrient relations and crop growth. With the aim of estimating rapeseed yield performance in response to fertilization in rice–rapeseed(RR) and cotton–rapeseed(CR) rotations, on-farm experiments were conducted at 70 sites across Hubei province, central China. The economically optimal fertilizer rates of winter oilseed rape in different rotations were determined. Field experiments showed that previous crops significantly influenced seed yields. Without N fertilization,seed yields were significantly lower for the RR rotation than for the CR rotation. The average yield increase ratio and agronomic efficiency associated with nitrogen(N)fertilization in the RR rotation were 96.6% and 6.56 kg kg- 1, significantly higher than those in the CR rotation. No seed yield differences were detected between the two rotations under phosphorus(P) and potassium(K) fertilization. In contrast to the CR rotation, N fertilizer played a more vital role in maintaining high seed yields in the RR rotation owing to the lower indigenous soil N supply. Compared with local N fertilizer recommendation rates for the RR rotation, on average an additional 18 kg N ha- 1was recommended according to the economically optimal N fertilizer rate(EONFR). In contrast, the EONFR was 14 kg N ha- 1lower than the locally recommended N fertilizer rate for the CR rotation. There were no differences between the two rotations for the average economically optimal P and K fertilization rates. Consequently, the average EONFR of winter oilseed rape could be reduced if cotton rather than rice preceded the winter oilseed rape.展开更多
The mixture of five yeast strains obtained from soil could remove about 85% TOC of oil rich wastewater in batch test. While the highest MLSS was obtained at an N∶C of 1∶5, the oil removal decreased with the increas...The mixture of five yeast strains obtained from soil could remove about 85% TOC of oil rich wastewater in batch test. While the highest MLSS was obtained at an N∶C of 1∶5, the oil removal decreased with the increase of N∶C during yeast sludge cultivation. Ammonium chloride was the best nitrogen source for yeast cultivation from the viewpoint of yeast growth and oil utilization. An ammonia concentration of over 1300 mg/L led to mass death of yeast at a pH of 5. The ammonia concentration should be controlled at a level of 1000 mg/L or lower.展开更多
Seeds are a source of organic (carbon, C) and mineral (nitrogen, N and phosphorus, P) nutrients for the growing seedling. There is much information on seed mass and N and P contents, and the relationship between these...Seeds are a source of organic (carbon, C) and mineral (nitrogen, N and phosphorus, P) nutrients for the growing seedling. There is much information on seed mass and N and P contents, and the relationship between these and seedling mass. Within the world’s temperate regions, these collectively show that N and P concentrations remain constant or rise with increase in seed mass and that seeds are larger and more nutrient-enriched in poorer soils. Seed N and P were more important than seed C in accounting for seedling mass in 85% of studies we assessed. In nutrient- and water-limited environments that are not light-limited, large seeds routinely provision the seedling with N and P that enhance C-fixation and thus general growth in the first wet season. This system is so efficient that growth response to soil nutrients may be negligible in first-year seedlings arising from seeds > 15 mg mass, N content > 5 mg and P content > 1.6 mg. The elongating taproot system absorbs nutrients and maintains water uptake as soil water retreats, enhancing the chances of survival in the first dry season. We outline an interpretative scenario for the special role of large seeds (>15 mg) in nutrient- and water-limited environments that recognizes the critical role of N and P for photosynthesis in ensuring sufficient C-supply to the rapidly descending roots for effective drought-avoidance by the young plant.展开更多
Effects of crop rotation on soybean (Glycine max (L) Merr.) seed composition have not been well investigated. Therefore, the objective of this study was to investigate the effects of soybean-corn (Zea mays L.) rotatio...Effects of crop rotation on soybean (Glycine max (L) Merr.) seed composition have not been well investigated. Therefore, the objective of this study was to investigate the effects of soybean-corn (Zea mays L.) rotations on seed protein, oil, and fatty acids composition on soybean. Soybeans were grown at Stoneville, MS, from 2005 to 2008 in five different scheduled cropping sequences. In 2007, following three years of rotation with corn, seed oleic acid percentage was significantly higher in any crop rotation than continuous soybean. The increase of oleic fatty acid ranged from 61 to 68% in 2007, and from 27 to 51% in 2008, depending on the rotation. The increase of oleic acid was accompanied by significant increases in seed concentrations of phosphorus (P), iron (Fe), and boron (B). In 2007, the increase of P ranged from 60 to 75%, Fe from 70 to 72%, and B from 34 to 69%. In 2008, the increase of P ranged from 82 to 106%, Fe from 32 to 84%, and B from 62 to 77%. Continuous soybean had higher linoleic:oleic ratio and linoleic: palmitic + stearic + oleic ratio, indicating that relative quantity of linoleic acid decreased in rotated crops. The total production of protein, oil, stearic and oleic fatty acids was the lowest in continuous soybean. The total production of palmitic acid was inconsistent across years. The results show that soybean- corn rotation affects seed composition by consistently increasing seed oleic fatty acid, P, Fe, and B concentrations. Higher oleic acid, unsaturated fatty acid, is desirable for oil stability and long-shelf storage. The mechanisms of how these nutrients are involved are not yet understood.展开更多
Objective:To study asymbiotic seed germination and mass propagation of Dendrobium lasianthera which is one of the endangered medicinal orchids using seeds.Methods:The 14 weeks old hand pollinated seeds were sown on Va...Objective:To study asymbiotic seed germination and mass propagation of Dendrobium lasianthera which is one of the endangered medicinal orchids using seeds.Methods:The 14 weeks old hand pollinated seeds were sown on Vacin and Went(VW)solid medium supplemented with various concentrations of peptone(1,2,3 g/L) and without peptone which was used as control treatment.At the 4,8,and 12 weeks after the seeds were sown,seed germination and shoot formation were investigated.To evaluate the role of organic nutrient additives on subsequent shoot development and root formation,particular shoots with about 1 cm length contains 1–2 leaves obtained from the seeds germination was cultured on VW medium additives with different of organic nutrient:15% coconut water,2 g/L peptone,150 g/L banana homogenate,and without organic nutrient was used as control.After 16 weeks of culture,the plantlet height,number of leaves,number of roots,leaf length and root length were recorded.Results:The supplementation of 2 g/L peptone in VW medium was proven to be suitable concentration for seed germination(100%) and shoot formation with 84.0% the protocorm development to phase 5(shoot).VW medium containing 15% coconut water was effectively improved the shoot development,with well developed roots and leaves compared to the other treatment and 95% of acclimatized plantlets survived.Conclusions:This protocol is an efficient way for the in vitro mass propagation of this Dendrobium lasianthera.展开更多
Endomycorrhizal fungi play an important role in the survival of plants on poor soils. Planting seeds into lunar soil at a lunar colony will be a challenge for seeds of any plant. The seeds will need a special microbi...Endomycorrhizal fungi play an important role in the survival of plants on poor soils. Planting seeds into lunar soil at a lunar colony will be a challenge for seeds of any plant. The seeds will need a special microbial “tool kit” that will help them germinate and the young seedlings establish themselves. In this study, seeds of the prickly pear cactus, Opuntia ficus-indica, were chosen to examine the presence of fungus spores in the soil, inside the seeds and after germination in the rhizosphere, roots and other tissues of the young seedlings. The nutrient poor lunar regolith simulant JSC-1A was used as autoclaved or untreated growth medium. The mycorrhizal fungus Trichoderma viride was predominantly identified on the roots of new seedlings. This fungus also demonstrated the strongest effect on the germination rate of the seeds in comparison with other fungi isolated from the rhizosphere of Opuntia plants. T. viride was not detected within seeds and also not within seedlings, besides the root tips, whereas an arbuscular mycorrhizal Glomus species was seed-borne and present throughout most of the seedling. A close association between T. viride and a Glomus species associated with O. ficus-indica is demonstrated through light microscopic and electron microscopic images of the outside and inside root tips of the seedlings.展开更多
The predicated changes in precipitation and temperature associated with the continued elevation of atmospheric CO2 concentration will trigger the northward shift of the Climate Envelopes for 130 North America tree spe...The predicated changes in precipitation and temperature associated with the continued elevation of atmospheric CO2 concentration will trigger the northward shift of the Climate Envelopes for 130 North America tree species by as much as 10 degrees. However, climate envelope models do not take into account changes in other factors that may also influence the survival and growth of plants at the predicted new locations, such as photoperiod and nutrient regimes. This study investigated how photoperiod and nitrogen supply would affect the ecophysiological traits of black spruce (Picea mariana (Mill) B. S. P.) that are critical for survival and growth at new locations predicted by climate envelope models. We exposed black spruce seedlings to the photoperiod regime at the seed origin (PS) and that 10° north of the seed origin (PNM) as predicted by climate envelope models under the current and doubled atmospheric CO2 concentration and different levels of N supply (30 vs. 300 μmol·mol-1 N). We found that the PNM and the 30 μmol·mol-1 N supply both had negative impact on the development of seedling cold hardiness in the fall, and led to earlier burst of the terminal bud and greater rate of mortality in the following growing season. While the PNM stimulated seedling growth in the first growing season, the effect was not sustained in the second growing season. Our results suggest that the photoperiod regimes and poor nutrient conditions at higher latitudes will likely constrain the scope of the northward migration or seed transfer of black spruce.展开更多
基金supported by the National Key Technologies R&D Program of China during the 11th Five-Year Plan period (2010BAD01B05)the Earmarked Fund for China Agriculture Research System (CARS-13)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in University of China (IRT1247)the Fundamental Research Funds for the Central Universities,China (2013PY113)
文摘Direct-sowing establishment method has great significance in improving winter oilseed rape(Brassica napus L.) production and guaranteeing edible oil security in China. However, nutrient responses on direct sown winter oilseed rape(DOR) performance and population development dynamic are still not well understood. Therefore, five on-farm experiments were conducted in the reaches of the Yangtze River(RYR) to determine the effects of nitrogen(N), phosphorus(P), and potassium(K) deficiencies on population density, dry matter production, nutrient uptake, seed yield, and yield components of DOR plants. Four fertilization treatments included the balanced NPK application treatment(NPK, 180 kg N, 39.3 kg P, 100 kg K, and 1.8 kg borax ha–1) and three nutrient deficiency treatments based on the NPK treatment, i.e., –N, –P, and –K. The results indicated that DOR population density declined gradually throughout the growing season, especially at over-wintering and pod-development stages. Nutrient deficiency decreased nutrient concentration in DOR plants, limited dry matter production and nutrient uptake, and thereby exacerbated density reduction during plants growth. The poor individual growth and reduced population density together decreased seed yield in the nutrient deficiency treatment. Averaged across all the experiments, seed yield reduced 61% by N deficiency, 38.3% by P deficiency, and 14.4% by K deficiency. The negative effects of nutrient deficiency on DOR performances followed the order of –N–P–K, and the effects were various among different nutrient deficiencies. Although N deficiency improved DOR emergence, but it seriously limited dry matter production and nutrient uptake, which in turn led to substantial plants death and therefore resulted in a very low harvested density. The P deficiency significantly reduced initial density, limited plants growth, and exacerbated density reduction. The K deficiency mainly decreased individual growth and yield, but did not affect density dynamic. Our results highlighted the importance of balanced NPK application in DOR production, suggesting that management strategy of these nutrients should be comprehensively considered with an aim to build an appropriate population structure with balanced plant density and individual growth.
文摘The proper use of organic and inorganic nutrient sources is important to sustain high levels of crop production, while maintaining or enhancing soil and environmental quality. A 4-year (2009 to 2012) field experiment was established in spring 2009 on a Gray Luvisol (Typic Haplocryalf) loam soil at Star City, Saskatchewan, Canada, to determine the effectiveness of organic/biological (compost, wood ash [fine and granular], alfalfa pellets, distiller grain, thin stillage, glycerol, fish food additive, Penicillium bilaiae), inorganic/mineral (granular-gypsum, rapid release elemental S [RRES], rock phosphate [granular and fine]) and chemical/synthetic (granular-ammonium nitrate, triple super phosphate and potassium sulphate) nutrient sources (amendments/chemicals) in improving seed yield, straw yield, seed quality and nutrient uptake (N, P, K and S) in seed + straw of canola. Combined application of N, P and S chemical fertilizers (NPS) produced considerably greater seed yield, straw yield and nutrient uptake of canola compared to the unamended control in all four years. In treatments receiving only organic amendments, thin stillage produced the greatest seed yield, straw yield and nutrient uptake in all years, and it was similar to the NPS balanced fertilizer treatment, while fish food additive and distiller grain dry of wheat in 2009, 2011 and 2012, distiller grain dry of corn in 2009 and 2012, and compost and alfalfa pellets in 2011 and 2012 produced significantly greater seed yield, straw yield and nutrient uptake, when compared to the control. In treatments where chemical fertilizers were also applied, in addition to organic amendments, ap- plication of N fertilizer increased seed yield, straw yield and nutrient uptake substantially when combined with wood ash fine in 2009, 2010, 2011 and 2012, wood ash granular in 2009, 2011 and 2012, and glycerol in 2009 and 2012 (moderate increase in 2012). In the chemical fertilizer treatments, there was a reduction in seed yield, straw yield and nutrient uptake of canola when only N fertilizer was applied compared to the control (significant in 2010 and 2011). Application of P along with N (NP) increased seed yield, straw yield and nutrient uptake of canola compared to N alone treatment, but was less than the NPS treatment in all years. Application of S along with N (NS) increased seed yield, straw yield and nutrient uptake of canola further compared to the NP treatment, but it was still lower than the NPS treatment in 2010 and 2011. In treatments receiving inorganic/mineral amendments in addition to chemical fertilizers, application of N + P fertilizers substantially increased seed yield, straw yield and nutrient uptake in treatments receiving gypsum and RRES in 2009, 2010, 2011 and 2012. This suggests the potential of gypsum and RRES in preventing S deficiency in organic crops when grown on S-deficient soils, provided other nutrients are not limiting in the soil for crop growth. Seed yield, straw yield and nutrient uptake with application of N and S fertilizers in combination with rock phosphate and/or Penicillium bilaiae were similar to N + S treatment in most cases, except in 2011 when application of finely-ground or powder rock phosphate in a combination with N + S produced significantly greater yield and nutrient uptake than N + S with granular rock phosphate. This suggests little contribution of rock phosphate and/or Penicillium bilaiae in improving yield and nutrient uptake of canola, and improves the performance of fine rock phosphate only evident in the third growing season in 2011, after three consecutive applications, but not in 2012. In conclusion, some organic amendments showed potential for improvement in organic crop production, and in some other cases highest yield and nutrient uptake were
文摘Cotton(Gossypium spp.) is the leading fiber crop,and an important source of the important edible oil and protein meals in the world.Complex genetics and strong environmental effects hinder
文摘Two field studies were carried out using Solanum macrocarpon and Solanum scabrum to investigate the effects of planting methods and seed density required for optimum biomass yield and nutrient content in southwest Nigeria. Two planting methods (drilling and broadcasting), and two seed densities (4 and 8 table spoon full) were investigated as a 2×2×3 factorial experiment in randomized complete block design and replicated three times. Four seed spoons gave 25.78 kg seeds ha-1 for S. macrocarpon and 20.67 kg·seeds·ha-1 for S. scabrum. Significant highest S. macrocarpon shoots (2.75 kg·m-2) and Ca, K, Fe and Zn contents and S. scabrum (2.95 kg·m-2) and Zn content, were obtained with drilling and four spoons of seeds compared with broadcasting and 8 spoons of seeds. This treatment gave average values for N, P, K, Mg, Ca, Na, Fe, Zn, Mn, Cu, Ca/P and Na/K ratio were 2.79%, 0.03%, 3.37%, 1.64%, 0.02%, 300 mg·kg-1, 100 mg·kg-1, 300 mg·kg-1, 8 mg·kg-1, 1.22 and 0.008 for S. macrocarpon and 2.05%, 0.27%, 5.81%, 3.27%, 2.91%, 0.09%, 280 mg·kg-1, 52.99 mg·kg-1, 359.35 mg·kg-1 and 22.42 mg·kg-1, 11.63 and 0.016 for S. scabrum. It was concluded that planting in drilling made weeding, fertilizer application, irrigation and harvesting more effective rather than by broadcasting, and four spoons of seeds per 9 m-2 produced deep green and broader leaves and balanced nutrient contents than eight spoons of seeds.
基金supported by the National Natural Science Foundation of China(41401324)the earmarked fund for China Agriculture Research System(CARS-13)+1 种基金the Fundamental Research Funds for the Central Universities(2013QC045)the Program for Changjiang Scholars and Innovative Research Team in University of China(IRT1247)
文摘Differences in soil physical, chemical and biological properties between paddy–upland and continuous upland rotations will influence nutrient relations and crop growth. With the aim of estimating rapeseed yield performance in response to fertilization in rice–rapeseed(RR) and cotton–rapeseed(CR) rotations, on-farm experiments were conducted at 70 sites across Hubei province, central China. The economically optimal fertilizer rates of winter oilseed rape in different rotations were determined. Field experiments showed that previous crops significantly influenced seed yields. Without N fertilization,seed yields were significantly lower for the RR rotation than for the CR rotation. The average yield increase ratio and agronomic efficiency associated with nitrogen(N)fertilization in the RR rotation were 96.6% and 6.56 kg kg- 1, significantly higher than those in the CR rotation. No seed yield differences were detected between the two rotations under phosphorus(P) and potassium(K) fertilization. In contrast to the CR rotation, N fertilizer played a more vital role in maintaining high seed yields in the RR rotation owing to the lower indigenous soil N supply. Compared with local N fertilizer recommendation rates for the RR rotation, on average an additional 18 kg N ha- 1was recommended according to the economically optimal N fertilizer rate(EONFR). In contrast, the EONFR was 14 kg N ha- 1lower than the locally recommended N fertilizer rate for the CR rotation. There were no differences between the two rotations for the average economically optimal P and K fertilization rates. Consequently, the average EONFR of winter oilseed rape could be reduced if cotton rather than rice preceded the winter oilseed rape.
文摘The mixture of five yeast strains obtained from soil could remove about 85% TOC of oil rich wastewater in batch test. While the highest MLSS was obtained at an N∶C of 1∶5, the oil removal decreased with the increase of N∶C during yeast sludge cultivation. Ammonium chloride was the best nitrogen source for yeast cultivation from the viewpoint of yeast growth and oil utilization. An ammonia concentration of over 1300 mg/L led to mass death of yeast at a pH of 5. The ammonia concentration should be controlled at a level of 1000 mg/L or lower.
文摘Seeds are a source of organic (carbon, C) and mineral (nitrogen, N and phosphorus, P) nutrients for the growing seedling. There is much information on seed mass and N and P contents, and the relationship between these and seedling mass. Within the world’s temperate regions, these collectively show that N and P concentrations remain constant or rise with increase in seed mass and that seeds are larger and more nutrient-enriched in poorer soils. Seed N and P were more important than seed C in accounting for seedling mass in 85% of studies we assessed. In nutrient- and water-limited environments that are not light-limited, large seeds routinely provision the seedling with N and P that enhance C-fixation and thus general growth in the first wet season. This system is so efficient that growth response to soil nutrients may be negligible in first-year seedlings arising from seeds > 15 mg mass, N content > 5 mg and P content > 1.6 mg. The elongating taproot system absorbs nutrients and maintains water uptake as soil water retreats, enhancing the chances of survival in the first dry season. We outline an interpretative scenario for the special role of large seeds (>15 mg) in nutrient- and water-limited environments that recognizes the critical role of N and P for photosynthesis in ensuring sufficient C-supply to the rapidly descending roots for effective drought-avoidance by the young plant.
文摘Effects of crop rotation on soybean (Glycine max (L) Merr.) seed composition have not been well investigated. Therefore, the objective of this study was to investigate the effects of soybean-corn (Zea mays L.) rotations on seed protein, oil, and fatty acids composition on soybean. Soybeans were grown at Stoneville, MS, from 2005 to 2008 in five different scheduled cropping sequences. In 2007, following three years of rotation with corn, seed oleic acid percentage was significantly higher in any crop rotation than continuous soybean. The increase of oleic fatty acid ranged from 61 to 68% in 2007, and from 27 to 51% in 2008, depending on the rotation. The increase of oleic acid was accompanied by significant increases in seed concentrations of phosphorus (P), iron (Fe), and boron (B). In 2007, the increase of P ranged from 60 to 75%, Fe from 70 to 72%, and B from 34 to 69%. In 2008, the increase of P ranged from 82 to 106%, Fe from 32 to 84%, and B from 62 to 77%. Continuous soybean had higher linoleic:oleic ratio and linoleic: palmitic + stearic + oleic ratio, indicating that relative quantity of linoleic acid decreased in rotated crops. The total production of protein, oil, stearic and oleic fatty acids was the lowest in continuous soybean. The total production of palmitic acid was inconsistent across years. The results show that soybean- corn rotation affects seed composition by consistently increasing seed oleic fatty acid, P, Fe, and B concentrations. Higher oleic acid, unsaturated fatty acid, is desirable for oil stability and long-shelf storage. The mechanisms of how these nutrients are involved are not yet understood.
基金Supported by the Decentralized Research Program Directorate General Higher Education Indonesia No.519/UN3/2015
文摘Objective:To study asymbiotic seed germination and mass propagation of Dendrobium lasianthera which is one of the endangered medicinal orchids using seeds.Methods:The 14 weeks old hand pollinated seeds were sown on Vacin and Went(VW)solid medium supplemented with various concentrations of peptone(1,2,3 g/L) and without peptone which was used as control treatment.At the 4,8,and 12 weeks after the seeds were sown,seed germination and shoot formation were investigated.To evaluate the role of organic nutrient additives on subsequent shoot development and root formation,particular shoots with about 1 cm length contains 1–2 leaves obtained from the seeds germination was cultured on VW medium additives with different of organic nutrient:15% coconut water,2 g/L peptone,150 g/L banana homogenate,and without organic nutrient was used as control.After 16 weeks of culture,the plantlet height,number of leaves,number of roots,leaf length and root length were recorded.Results:The supplementation of 2 g/L peptone in VW medium was proven to be suitable concentration for seed germination(100%) and shoot formation with 84.0% the protocorm development to phase 5(shoot).VW medium containing 15% coconut water was effectively improved the shoot development,with well developed roots and leaves compared to the other treatment and 95% of acclimatized plantlets survived.Conclusions:This protocol is an efficient way for the in vitro mass propagation of this Dendrobium lasianthera.
文摘Endomycorrhizal fungi play an important role in the survival of plants on poor soils. Planting seeds into lunar soil at a lunar colony will be a challenge for seeds of any plant. The seeds will need a special microbial “tool kit” that will help them germinate and the young seedlings establish themselves. In this study, seeds of the prickly pear cactus, Opuntia ficus-indica, were chosen to examine the presence of fungus spores in the soil, inside the seeds and after germination in the rhizosphere, roots and other tissues of the young seedlings. The nutrient poor lunar regolith simulant JSC-1A was used as autoclaved or untreated growth medium. The mycorrhizal fungus Trichoderma viride was predominantly identified on the roots of new seedlings. This fungus also demonstrated the strongest effect on the germination rate of the seeds in comparison with other fungi isolated from the rhizosphere of Opuntia plants. T. viride was not detected within seeds and also not within seedlings, besides the root tips, whereas an arbuscular mycorrhizal Glomus species was seed-borne and present throughout most of the seedling. A close association between T. viride and a Glomus species associated with O. ficus-indica is demonstrated through light microscopic and electron microscopic images of the outside and inside root tips of the seedlings.
文摘The predicated changes in precipitation and temperature associated with the continued elevation of atmospheric CO2 concentration will trigger the northward shift of the Climate Envelopes for 130 North America tree species by as much as 10 degrees. However, climate envelope models do not take into account changes in other factors that may also influence the survival and growth of plants at the predicted new locations, such as photoperiod and nutrient regimes. This study investigated how photoperiod and nitrogen supply would affect the ecophysiological traits of black spruce (Picea mariana (Mill) B. S. P.) that are critical for survival and growth at new locations predicted by climate envelope models. We exposed black spruce seedlings to the photoperiod regime at the seed origin (PS) and that 10° north of the seed origin (PNM) as predicted by climate envelope models under the current and doubled atmospheric CO2 concentration and different levels of N supply (30 vs. 300 μmol·mol-1 N). We found that the PNM and the 30 μmol·mol-1 N supply both had negative impact on the development of seedling cold hardiness in the fall, and led to earlier burst of the terminal bud and greater rate of mortality in the following growing season. While the PNM stimulated seedling growth in the first growing season, the effect was not sustained in the second growing season. Our results suggest that the photoperiod regimes and poor nutrient conditions at higher latitudes will likely constrain the scope of the northward migration or seed transfer of black spruce.