以野生大豆ZYD00006为供体亲本,黑龙江省主栽品种绥农14为轮回亲本,连续多年回交并自交,构建了高世代染色体片段代换系BC3F3代161个株行。该群体经多代回交的遗传背景相对一致,大大提高了QTL定位的准确度。结合单因素方差分析法和独立样...以野生大豆ZYD00006为供体亲本,黑龙江省主栽品种绥农14为轮回亲本,连续多年回交并自交,构建了高世代染色体片段代换系BC3F3代161个株行。该群体经多代回交的遗传背景相对一致,大大提高了QTL定位的准确度。结合单因素方差分析法和独立样本T检验法对群体进行QTL定位,共获得9个单株粒重的QTL,分布于7个连锁群。两种方法中均被检测到的有3个QTL,分别为QSW-J-1、QSW-J-2和QSW-G-1;QSW-G-1和QSW-G-2与已有研究结果相吻合;其余7个QTL为新发现QTL,可能是本材料特有位点;其中QSW-J-1的导入片段长度是7.0 c M,且加性效应值为-2.7 g,可作为继续研究的首选位点。展开更多
Drought stress is an important factor affecting soybean yield.Improving drought tolerance of soybean varieties can increase yield and yield stability when the stress occurs.Identifying QTL related to drought tolerance...Drought stress is an important factor affecting soybean yield.Improving drought tolerance of soybean varieties can increase yield and yield stability when the stress occurs.Identifying QTL related to drought tolerance using molecular marker-assisted selection is able to facilitate the development of drought-tolerant soybean varieties.In this study,we used a high-yielding and drought-sensitive cultivar‘Zhonghuang 35’and a drought-tolerant cultivar‘Jindou 21’to establish F6:9 recombinant inbred lines.We constructed a highdensity genetic map using specific locus amplified fragment sequencing(SLAF-Seq)technology.The genetic map contained 8078 SLAF markers distributing across 20 soybean chromosomes with a total genetic distance of 3780.98 c M and an average genetic distance of0.59 c M between adjacent markers.Two treatments(irrigation and drought)were used in the field tests,the Additive-Inclusive Composite Interval Mapping(ICIM-ADD)was used to call QTL,and plant height and seed weight per plant were used as the indicators of drought tolerance.We identified a total of 23 QTL related to drought tolerance.Among them,seven QTL(q PH2,q PH6,q PH7,q PH17,q PH19-1,q PH19-2,and q PH19-3)on chromosomes 2,6,7,17,and 19 were related to plant height,and five QTL(q SWPP2,q SWPP6,q SWPP13,q SWPP17,and q SWPP19)on chromosomes 2,6,13,17,and 19 were related to seed weight and could be considered as the major QTL.In addition,three common QTL(q PH6/q SWPP6,q PH17/q SWPP17,and q PH19-3/q SWPP19)for both plant height and seed weight per plant were located in the same genomic regions on the same chromosomes.Three(q PH2,q PH17,and q PH19-2)and four novel QTL(q SWPP2,q SWPP13,q SWPP17,and q SWPP19)were identified for plant height and seed weight per plant,respectively.Two pairs of QTL(q PH2/q SWPP2 and q PH17/q SWPP17)were also common for both plant height and seed weight per plant.These QTL and closely linked SLAF markers could be used to accelerate breeding for drought tolerant cultivars via MAS.展开更多
文摘以野生大豆ZYD00006为供体亲本,黑龙江省主栽品种绥农14为轮回亲本,连续多年回交并自交,构建了高世代染色体片段代换系BC3F3代161个株行。该群体经多代回交的遗传背景相对一致,大大提高了QTL定位的准确度。结合单因素方差分析法和独立样本T检验法对群体进行QTL定位,共获得9个单株粒重的QTL,分布于7个连锁群。两种方法中均被检测到的有3个QTL,分别为QSW-J-1、QSW-J-2和QSW-G-1;QSW-G-1和QSW-G-2与已有研究结果相吻合;其余7个QTL为新发现QTL,可能是本材料特有位点;其中QSW-J-1的导入片段长度是7.0 c M,且加性效应值为-2.7 g,可作为继续研究的首选位点。
基金supported by the National Key Research and Development Program of China(2016YFD0100201 and 2016YFD0100304)the National Science and Technological Innovation Program of China。
文摘Drought stress is an important factor affecting soybean yield.Improving drought tolerance of soybean varieties can increase yield and yield stability when the stress occurs.Identifying QTL related to drought tolerance using molecular marker-assisted selection is able to facilitate the development of drought-tolerant soybean varieties.In this study,we used a high-yielding and drought-sensitive cultivar‘Zhonghuang 35’and a drought-tolerant cultivar‘Jindou 21’to establish F6:9 recombinant inbred lines.We constructed a highdensity genetic map using specific locus amplified fragment sequencing(SLAF-Seq)technology.The genetic map contained 8078 SLAF markers distributing across 20 soybean chromosomes with a total genetic distance of 3780.98 c M and an average genetic distance of0.59 c M between adjacent markers.Two treatments(irrigation and drought)were used in the field tests,the Additive-Inclusive Composite Interval Mapping(ICIM-ADD)was used to call QTL,and plant height and seed weight per plant were used as the indicators of drought tolerance.We identified a total of 23 QTL related to drought tolerance.Among them,seven QTL(q PH2,q PH6,q PH7,q PH17,q PH19-1,q PH19-2,and q PH19-3)on chromosomes 2,6,7,17,and 19 were related to plant height,and five QTL(q SWPP2,q SWPP6,q SWPP13,q SWPP17,and q SWPP19)on chromosomes 2,6,13,17,and 19 were related to seed weight and could be considered as the major QTL.In addition,three common QTL(q PH6/q SWPP6,q PH17/q SWPP17,and q PH19-3/q SWPP19)for both plant height and seed weight per plant were located in the same genomic regions on the same chromosomes.Three(q PH2,q PH17,and q PH19-2)and four novel QTL(q SWPP2,q SWPP13,q SWPP17,and q SWPP19)were identified for plant height and seed weight per plant,respectively.Two pairs of QTL(q PH2/q SWPP2 and q PH17/q SWPP17)were also common for both plant height and seed weight per plant.These QTL and closely linked SLAF markers could be used to accelerate breeding for drought tolerant cultivars via MAS.
