Background:This study aims to explore the therapeutic effects of the Qi Teng Mai Ning recipe on ischemic stroke and vascular cognitive impairment through its potential to modulate cellular autophagy,with a focus on id...Background:This study aims to explore the therapeutic effects of the Qi Teng Mai Ning recipe on ischemic stroke and vascular cognitive impairment through its potential to modulate cellular autophagy,with a focus on identifying its active ingredients and their target proteins.Methods:The study began with the identification of active ingredients in the Qi Teng Mai Ning recipe.It proceeded to screen the gene expression omnibus database for ischemic stroke and vascular cognitive impairment-associated differentially expressed mRNAs and to identify cellular autophagy-related proteins via the Human Autophagy Database.These proteins were annotated with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functions and subjected to molecular docking with the recipe’s core active ingredients.In vitro cell experiments were conducted on hypoxic HT22 cells,involving CCK8 assay,lentiviral transfection to silence autophagy related 9B(ATG9B),immunofluorescence staining,and qPCR validation to investigate the effects of the recipe on autophagy.Results:The analysis identified 104 active ingredients targeting 408 proteins and forming a complex ingredient-target network.Intersecting 55 ischemic stroke-related and 909 vascular cognitive impairment-related differentially expressed mRNAs revealed 14 co-expressed mRNAs.Molecular docking showed quercetin,kaempferol,myrcene,and conferone as key ingredients targeting autophagy-related proteins.Cellular experiments indicated that the recipe significantly enhanced cell viability under hypoxic conditions,reduced apoptosis,and modulated the expression of autophagy-related factors,thereby decreasing apoptosis rates in HT22 cells.Conclusion:The Qi Teng Mai Ning recipe offers protective effects against ischemic stroke and vascular cognitive impairment by modulating autophagy-related proteins.Its efficacy highlights the potential of traditional Chinese medicine in treating these conditions,though further research is needed to fully understand its mechanisms and clinical applications.展开更多
The present study examines the thermal distribution of ternary nanofluid flow amid two spinning disks influenced by electric and magnetic fields. Keeping in view the shape of the particles, the electrically conducting...The present study examines the thermal distribution of ternary nanofluid flow amid two spinning disks influenced by electric and magnetic fields. Keeping in view the shape of the particles, the electrically conducting ternary nanofluid is analyzed with variable thermophysical features. Three types of nanoparticles namely Copper, Aluminum Oxide, and Graphene with spherical, cylindrical, and platelet shapes are taken respectively and are immersed in a (50-50)% ratio of water and ethylene glycol mixture which acts as a base fluid. The anticipated problem is addressed by employing a reliable and user-friendly numerical bvp4c built-in collocation scheme. This solution is then showcased through illustrations and tables. Strengthening the radiation results in an enhanced heat transfer rate. Radial and azimuthal velocities once rotation of disks is enhanced. The key findings provide a strong theoretical background in photovoltaic cells, solar collectors, radiators, solar water heaters, and many other applications.展开更多
文摘Background:This study aims to explore the therapeutic effects of the Qi Teng Mai Ning recipe on ischemic stroke and vascular cognitive impairment through its potential to modulate cellular autophagy,with a focus on identifying its active ingredients and their target proteins.Methods:The study began with the identification of active ingredients in the Qi Teng Mai Ning recipe.It proceeded to screen the gene expression omnibus database for ischemic stroke and vascular cognitive impairment-associated differentially expressed mRNAs and to identify cellular autophagy-related proteins via the Human Autophagy Database.These proteins were annotated with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functions and subjected to molecular docking with the recipe’s core active ingredients.In vitro cell experiments were conducted on hypoxic HT22 cells,involving CCK8 assay,lentiviral transfection to silence autophagy related 9B(ATG9B),immunofluorescence staining,and qPCR validation to investigate the effects of the recipe on autophagy.Results:The analysis identified 104 active ingredients targeting 408 proteins and forming a complex ingredient-target network.Intersecting 55 ischemic stroke-related and 909 vascular cognitive impairment-related differentially expressed mRNAs revealed 14 co-expressed mRNAs.Molecular docking showed quercetin,kaempferol,myrcene,and conferone as key ingredients targeting autophagy-related proteins.Cellular experiments indicated that the recipe significantly enhanced cell viability under hypoxic conditions,reduced apoptosis,and modulated the expression of autophagy-related factors,thereby decreasing apoptosis rates in HT22 cells.Conclusion:The Qi Teng Mai Ning recipe offers protective effects against ischemic stroke and vascular cognitive impairment by modulating autophagy-related proteins.Its efficacy highlights the potential of traditional Chinese medicine in treating these conditions,though further research is needed to fully understand its mechanisms and clinical applications.
文摘The present study examines the thermal distribution of ternary nanofluid flow amid two spinning disks influenced by electric and magnetic fields. Keeping in view the shape of the particles, the electrically conducting ternary nanofluid is analyzed with variable thermophysical features. Three types of nanoparticles namely Copper, Aluminum Oxide, and Graphene with spherical, cylindrical, and platelet shapes are taken respectively and are immersed in a (50-50)% ratio of water and ethylene glycol mixture which acts as a base fluid. The anticipated problem is addressed by employing a reliable and user-friendly numerical bvp4c built-in collocation scheme. This solution is then showcased through illustrations and tables. Strengthening the radiation results in an enhanced heat transfer rate. Radial and azimuthal velocities once rotation of disks is enhanced. The key findings provide a strong theoretical background in photovoltaic cells, solar collectors, radiators, solar water heaters, and many other applications.
文摘为了促进区域配电网新能源电力的就地消纳、解决传统储能形式难以满足由新能源间断性发电带来的长时存储需求,考虑氢储能的多能联产联储特性,提出计及共享交易机制的多类型微网共享氢储能系统容量规划方法.考虑异质性微网的多种调节需求,设计由微电网群运营商配置氢储能的多微网能源交易基本框架.考虑氢储能在共享运营时与多微网间复杂的利益交互关系,计及微网内部的电热需求响应,提出基于主从博弈的共享氢储能交易定价机制,以保证共享模式的可持续发展.对共享氢储能的容量规划进行研究,降低微电网群运营商的投资成本,保证微电网群运营商的共享收益.算例分析结果表明,利用所提的容量规划方法,能够缩短微电网群运营商的氢储能投资回收年限2.36 a.
