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Seismic monitoring of sub-seafloor fluid processes in the Haima cold seep area using an Ocean Bottom Seismometer (OBS) 被引量:3
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作者 Bin Liu JianYu Huang +2 位作者 WenBin Jiang WeiWei Wang Li Yang 《Earth and Planetary Physics》 CAS CSCD 2023年第5期582-602,共21页
The use of ocean bottom seismometers provides an effective means of studying the process and the dynamic of cold seeps by continuously recording micro-events produced by sub-seafloor fluid migration.We deployed a four... The use of ocean bottom seismometers provides an effective means of studying the process and the dynamic of cold seeps by continuously recording micro-events produced by sub-seafloor fluid migration.We deployed a four-component Ocean Bottom Seismometer(OBS)at an active site of the Haima cold seep from 6 November to 19 November in 2021.Here,we present the results of this short-term OBS monitoring.We first examine the OBS record manually to distinguish(by their distinctive seismographic signatures)four types of events:shipping noises,vibrations from our remotely operated vehicle(ROV)operations,local earthquakes,and short duration events(SDEs).Only the SDEs are further discussed in this work.Such SDEs are similar to those observed in other sea areas and are interpreted to be correlated with sub-seafloor fluid migration.In the OBS data collected during the 14-day monitoring period.We identify five SDEs.Compared to the SDE occurrence rate observed in other cold seep regions,five events is rather low,from which it could be inferred that fluid migration,and subsequent gas seepage,is not very active at the Haima site.This conclusion agrees with multi-beam and chemical observations at that site.Our observations thus provide further constraint on the seepage activity in this location.This is the first time that cold seep-related SDEs have been identified in the South China Sea,expanding the list of sea areas where SDEs are now linked to cold seep fluid migration. 展开更多
关键词 cold seep OBS(Ocean Bottom Seismometer) SDE(short duration event) Haima South China Sea
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Interactions of Microplastics and Methane Seepage in the Deep-Sea Environment 被引量:1
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作者 Jing-Chun Feng Zhifeng Yang +8 位作者 Wenliang Zhou Xingwei Feng Fuwen Wei Bo Li Chuanxin Ma Si Zhang Linlin Xia Yanpeng Cai Yi Wang 《Engineering》 SCIE EI CAS CSCD 2023年第10期159-167,共9页
Microplastics(MPs)are important exempla of the Anthropocene and are exerting an increasing impact on Earth’s carbon cycle.The huge imbalance between the MPs floating on the marine surface and those that are estimated... Microplastics(MPs)are important exempla of the Anthropocene and are exerting an increasing impact on Earth’s carbon cycle.The huge imbalance between the MPs floating on the marine surface and those that are estimated to have been introduced into the ocean necessitates a detailed assessment of marine MP sinks.Here,we demonstrate that cold seep sediments,which are characterized by methane fluid seepage and a chemosynthetic ecosystem,effectively capture and accommodate small-scale(<100μm)MPs,with 16 types of MPs being detected.The abundance of MPs in the surface of the sediment is higher in methane-seepage locations than in non-seepage areas.Methane seepage is beneficial to the accumulation,fragmentation,increased diversity,and aging of MPs.In turn,the rough surfaces of MPs contribute to the sequestration of the electron acceptor ferric oxide,which is associated with the anaerobic oxidation of methane(AOM).The efficiency of the AOM determines whether the seeping methane(which has a greenhouse effect 83 times greater than that of CO_(2)over a 20-year period)can enter the atmosphere,which is important to the global methane cycle,since the deep-sea environment is regarded as the largest methane reservoir associated with natural gas hydrates. 展开更多
关键词 Microplastics Anaerobic oxidation of methane Cold seeps Diversity index FRAGMENTATION Gas hydrates
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Discovery of prenylated indole alkaloid and natural xanthone from cold-seep sediment derived fungus Talaromyces funiculosus SD-523
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作者 Yanan WANG Xiaoming LI +3 位作者 Fengyi LÜ Suiqun YANG Linghong MENG Bingui WANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第3期1152-1158,共7页
A new prenylated indole alkaloid 11,17-epi-mangrovamide A(1),a new natural occurring product,1,7-dihydroxy-6-methyl-8-hydroxymethyl-xanthone(2),two known alkaloids,mangrovamide A(3)and mangrovamide G(4),and four known... A new prenylated indole alkaloid 11,17-epi-mangrovamide A(1),a new natural occurring product,1,7-dihydroxy-6-methyl-8-hydroxymethyl-xanthone(2),two known alkaloids,mangrovamide A(3)and mangrovamide G(4),and four known polyketide derivatives(5–8)were isolated and identified from the cold-seep sediment derived fungal strain Talaromyces funiculosus SD-523.Their structures were elucidated by combination of nuclear magnetic resonance(NMR),high resolution electrospray ionization mass spectroscopy(HRESIMS),quantum chemical electronic circular dichroism(ECD),and DP4+probability analysis as well as by comparison of the data with literature reports.All isolated compounds were tested for antibacterial activities. 展开更多
关键词 Talaromyces funiculosus cold seep prenylated indole alkaloid antimicrobial activity
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Quantitative estimation of bubble volume fraction of submarine seep plumes by modeling seismic oceanography data
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作者 Tonggang HAN Jiangxin CHEN +3 位作者 Leonardo AZEVEDO Bingshou HE Huaning XU Rui YANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第2期673-686,共14页
Submarine seep plumes are a natural phenomenon in which different types of gases migrate through deep or shallow subsurface sediments and leak into seawater in pressure gradient.When detected using acoustic data,the l... Submarine seep plumes are a natural phenomenon in which different types of gases migrate through deep or shallow subsurface sediments and leak into seawater in pressure gradient.When detected using acoustic data,the leaked gases frequently exhibit a flame-like structure.We numerically modelled the relationship between the seismic response characteristic and bubble volume fraction to establish the bubble volume fraction in the submarine seep plume.Results show that our models are able to invert and predict the bubble volume fraction from field seismic oceanography data,by which synthetic seismic sections in different dominant frequencies could be numerically simulated,seismic attribute sections(e.g.,instantaneous amplitude,instantaneous frequency,and instantaneous phase)extracted,and the correlation between the seismic attributes and bubble volume fraction be quantitatively determined with functional equations.The instantaneous amplitude is positively correlated with bubble volume fraction,while the instantaneous frequency and bubble volume fraction are negatively correlated.In addition,information entropy is introduced as a proxy to quantify the relationship between the instantaneous phase and bubble volume fraction.As the bubble volume fraction increases,the information entropy of the instantaneous phase increases rapidly at the beginning,followed by a slight upward trend,and finally stabilizes.Therefore,under optimal noise conditions,the bubble volume fraction of submarine seep plumes can be inverted and predicted based on seismic response characteristics in terms of seismic attributes. 展开更多
关键词 seismic oceanography submarine seep plumes bubble volume fraction seismic response characteristics seismic attribute analysis quantitative analysis
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Mg/Ca,Ba/Ca,and S/Ca ratios as environmental and growth proxies for bivalve shells from the Haima cold seep,South China Sea
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作者 Jingya CAO Shengxiong YANG +2 位作者 Danling TANG Junxi FENG Jinqiang LIANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第2期660-672,共13页
Bivalve shell fossils,cemented by authigenic carbonates,are widely spread in the Haima cold seep,Qiongdongnan Basin of the South China Sea(SCS).In this study,we examined an element profile of Gigantidas platifrons in ... Bivalve shell fossils,cemented by authigenic carbonates,are widely spread in the Haima cold seep,Qiongdongnan Basin of the South China Sea(SCS).In this study,we examined an element profile of Gigantidas platifrons in the Haima cold seeps at a water depth of 1381 m.Based on the scanning electron microscope(SEM)analyses,the prismatic layer and nacreous layer were identified,which are characterized by prismatic structure and stratified structure,respectively.In addition,the profile can be subdivided into two parts:altered and unaltered zones.Laser inductively coupled plasma mass spectrometry(LA-ICP-MS)mapping shows that the element concentrations of the altered zones were influenced by the authigenic carbonate rocks,whereas the element concentrations of unaltered zones remain stable.In-situ X-ray diffraction(XRD)analyses show that the mineral constituent of the prismatic layer is mainly composed of aragonite.Along with the growth profile,Mg/Ca ratios of unaltered zones have minor variations,ranging 0.72-0.97 mmol/mol(mean=0.87 mmol/mol),with estimated temperatures of 3.8-4.1℃,indicating that the temperature of the surrounding seawater remains constant and agree with the measured data of 3.9℃which was conducted by a conductivity-temperature-depth system(CTD).The minor variations of Ba/Ca ratios(0.01-0.06 mmol/mol;mean=0.04 mmol/mol)indicate a relatively stabilized salinity of the surrounding seawater.S/Ca ratios show large variations of 0.04-4.15 mmol/mol(mean=1.37 mmol/mol).S/Ca ratios have regular variations which generally correspond to the variations of the Mg/Ca ratios,highlighting that the S/Ca ratios of bivalve shells show the potential to reflect the growth rate of the Gigantides.However,further studies should be carried out on the understanding of the links between the S/Ca ratios and seepage intensity of cold-seep fluids. 展开更多
关键词 Mg/Ca Ba/Ca and S/Ca ratios laser inductively coupled plasma mass spectrometry(LA-ICPMS) Gigantidas platifrons Haima cold seep South China Sea
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Seep/w在坝基墙幕结合防渗体系结构优化中的应用 被引量:4
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作者 毕明亮 燕乔 王立彬 《三峡大学学报(自然科学版)》 CAS 2010年第2期11-15,共5页
在墙幕结合的防渗体系中,上部防渗墙和下部帷幕灌浆的结构尺寸大多是凭经验取得的.文章主要从工程防渗效果及安全性、经济性等方面出发,应用渗流有限元数值模拟软件Seep/w,采用图解最大梯度法,对墙接幕防渗体系的结构尺寸进行优化,得出... 在墙幕结合的防渗体系中,上部防渗墙和下部帷幕灌浆的结构尺寸大多是凭经验取得的.文章主要从工程防渗效果及安全性、经济性等方面出发,应用渗流有限元数值模拟软件Seep/w,采用图解最大梯度法,对墙接幕防渗体系的结构尺寸进行优化,得出既能满足防渗要求,又能确保工程安全、经济的最优化深度. 展开更多
关键词 seep/w 墙幕结合 结构尺寸 优化 基础防渗
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基于SEEP/W的吊江岩水电站闸坝渗流稳定分析 被引量:5
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作者 陆美霞 任德记 王刚 《水利科技与经济》 2010年第2期224-226,共3页
针对吊江岩水电站首部枢纽布置和闸址处河床覆盖层复杂的地质条件,采用SEEP/W软件对闸基进行了渗流稳定性分析。结果表明,设计防渗体系是合理可行的,为闸坝稳定性提供科学依据。
关键词 seep/W 渗流 稳定性
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SEEPS基热塑性磁流变弹性体复合材料的制备、结构与性能 被引量:3
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作者 乔秀颖 卢秀首 +4 位作者 龚兴龙 杨涛 李伟 孙康 陈晓东 《磁性材料及器件》 北大核心 2013年第5期1-5,11,共6页
选用性能优良的聚(苯乙烯-b-乙烯-b-乙烯-丙烯-b-苯乙烯)(SEEPS)热塑性弹性体代替传统橡胶为基体、软磁性羰基铁粉为磁性填料,采用熔融共混技术制备得到弹性好、磁流变效应高的新型各向同性和各向异性SEEPS基热塑性磁流变弹性体(MRE)复... 选用性能优良的聚(苯乙烯-b-乙烯-b-乙烯-丙烯-b-苯乙烯)(SEEPS)热塑性弹性体代替传统橡胶为基体、软磁性羰基铁粉为磁性填料,采用熔融共混技术制备得到弹性好、磁流变效应高的新型各向同性和各向异性SEEPS基热塑性磁流变弹性体(MRE)复合材料。详细地介绍了两类材料的制备方法,系统地研究了基体分子量、羰基铁含量和外加磁场强度等因素对材料结构和性能的影响。羰基铁粉在各向同性MRE复合材料中呈现无规均匀分散,在各向异性MRE复合材料中呈现明显链状定向有序结构。高分子量基体吸油性优于低分子量基体,柔软度更高,加工性能更好,强度和韧性受高填充羰基铁的影响小,磁流变效应也更高。各向异性MRE比各向同性MRE具有更高的储能模量和磁流变效应。 展开更多
关键词 磁流变弹性体 热塑性弹性体 羰基铁 seepS 性能
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弹性体SEEPS增韧聚碳酸酯的研究 被引量:1
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作者 张海涛 曹亚 +4 位作者 陈金耀 何玉晖 Hideo Takamatsu Xiangsheng Zeng Noboru Higashida 《高分子材料科学与工程》 EI CAS CSCD 北大核心 2005年第6期209-212,共4页
研究了三种不同分子量三嵌段弹性体SEEPS(po lystyrene-b-po ly(ethy lene/propy lene)-b-po lystyrene)的加入对聚碳酸酯性能的影响。发现三种弹性体都能大大改善基体PC的缺口敏感性高的行为。虽然薄制品的缺口冲击强度随弹性体加入量... 研究了三种不同分子量三嵌段弹性体SEEPS(po lystyrene-b-po ly(ethy lene/propy lene)-b-po lystyrene)的加入对聚碳酸酯性能的影响。发现三种弹性体都能大大改善基体PC的缺口敏感性高的行为。虽然薄制品的缺口冲击强度随弹性体加入量的增加而降低,但厚制品的缺口冲击强度却大幅度提高,即使弹性体含量达到25%时,厚制品仍然具有优良的抗缺口冲击性能。SEM观察发现材料由原来的冲击波状脆性断裂转变为韧性断裂。SEEPS的加入还能明显改善体系的流变性和抗环境应力开裂性能。 展开更多
关键词 聚碳酸酯 增韧 热塑性弹性体seepS
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SEEP/W在基于非饱和土理论的公路排水设计计算中的应用 被引量:13
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作者 周奇 《中外公路》 北大核心 2009年第6期42-46,共5页
该文针对基于非饱和土理论的公路排水设计问题,介绍了基于有限元法的非饱和土水模拟软件SEEP/W,并用SEEP/W对实际道路渗流场进行了模拟。通过对比实测数据,认为SEEP/W可以很好地模拟复杂边界条件下的路基非饱和土水暂态流问题,并且认为... 该文针对基于非饱和土理论的公路排水设计问题,介绍了基于有限元法的非饱和土水模拟软件SEEP/W,并用SEEP/W对实际道路渗流场进行了模拟。通过对比实测数据,认为SEEP/W可以很好地模拟复杂边界条件下的路基非饱和土水暂态流问题,并且认为土水特征曲线、水力传导曲线和几何边界是模拟计算的关键参数。通过路基内部渗沟和排水层对路基水分运动影响的模拟计算,发现渗沟或集水管同排水层的共同使用可以有效地减少路基水分。 展开更多
关键词 seep/W 数值模拟 非饱和土 公路排水 渗沟
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基于渗流原理的既有建筑抗浮修复治理工程研究
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作者 张勇 谢湘平 +1 位作者 闫春岭 王保民 《四川建筑》 2024年第3期35-38,共4页
以河南省安阳工学院教学楼为研究对象,通过试验和数值模拟,并依据相关设计规范,研究了一种将沉沙集水坑和盲沟相结合的排水泄压方法。研究结果表明:针对极限水位和抗浮水位,盲沟渗流通道的存在可以显著降低沿渗流路径到达地下室地板的... 以河南省安阳工学院教学楼为研究对象,通过试验和数值模拟,并依据相关设计规范,研究了一种将沉沙集水坑和盲沟相结合的排水泄压方法。研究结果表明:针对极限水位和抗浮水位,盲沟渗流通道的存在可以显著降低沿渗流路径到达地下室地板的总水头及孔隙水压力;沉沙集水坑可有效观察排水情况,避免排水过程中堵塞,增大排水效率。沉沙集水坑和盲沟相结合的排水设计可有效缓解既有建筑物基底受到浮力的作用,提升既有建筑物抗浮能力。 展开更多
关键词 既有建筑 抗浮修复 沉沙集水坑 盲沟 seep/W
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SEEPS结构、性能及应用研究进展 被引量:7
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作者 贺小进 王雪 邱迎昕 《石化技术》 CAS 2019年第11期1-4,共4页
SEEPS相比于SEBS,有更高的拉伸强度,更好的锁油性,更平滑的表面,与聚乙烯更好的相容性,更好的低温性能。SEEPS相比SEPS有更高的强度。对国内外SEEPS的发展现状、加氢用催化剂、SEEPS的结构、种类及用途进行了介绍,SEEPS可广泛应用于聚... SEEPS相比于SEBS,有更高的拉伸强度,更好的锁油性,更平滑的表面,与聚乙烯更好的相容性,更好的低温性能。SEEPS相比SEPS有更高的强度。对国内外SEEPS的发展现状、加氢用催化剂、SEEPS的结构、种类及用途进行了介绍,SEEPS可广泛应用于聚合物共混、胶粘剂、塑料改性、减震材料及发泡材料等。在我国SEBS及SEPS已实现产业化的基础上建议加大SEEPS开发力度,争取早日工业化,填补我国在该领域的空白。 展开更多
关键词 seepS 结构 性能 应用
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基于SEEP/W分析土石坝渗流稳定 被引量:6
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作者 鲍呈苍 《中国水运(下半月)》 2011年第6期147-148,共2页
文中根据饱和-非饱和渗流遵循的达西定律,以压力水头为基本未知量,从固、液相质量守恒方程出发,推导二维饱和一非饱和渗流控制方程。利用SEEP/W对一些BL坝进行断面剖分,计算了大坝浸润线位置、等势线、渗透坡降等,很好地解决了土坡渗流... 文中根据饱和-非饱和渗流遵循的达西定律,以压力水头为基本未知量,从固、液相质量守恒方程出发,推导二维饱和一非饱和渗流控制方程。利用SEEP/W对一些BL坝进行断面剖分,计算了大坝浸润线位置、等势线、渗透坡降等,很好地解决了土坡渗流和稳定分析结果相结合的问题。 展开更多
关键词 非饱和 seep/W 渗流
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SEEP2D软件在坝堤渗漏计算中的应用 被引量:3
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作者 冯西洲 《地下水》 2010年第3期139-140,共2页
在国际通用的地下水数值模拟软件中,GMS软件以其友好的使用界面,强大的前、后处理功能及其良好的三维可视效果应用越来越广泛。在对GMS软件中SEEP2D模块进行简单介绍的基础上,以坝堤剖面渗漏为例介绍SEEP2D模块在地下水数值模拟计算中... 在国际通用的地下水数值模拟软件中,GMS软件以其友好的使用界面,强大的前、后处理功能及其良好的三维可视效果应用越来越广泛。在对GMS软件中SEEP2D模块进行简单介绍的基础上,以坝堤剖面渗漏为例介绍SEEP2D模块在地下水数值模拟计算中的应用。 展开更多
关键词 GMS 数值模拟 seep2D 坝堤渗漏
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SEEP/W与SLOPE/W耦合的边坡稳定分析 被引量:4
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作者 王和平 陈剑平 刘炜 《人民长江》 北大核心 2016年第S1期120-123,共4页
为更准确地分析降雨条件下边坡的稳定性,以大连变电站拟开挖边坡为实例,运用SEEP/W分析了不同持时和降雨强度条件下边坡内部孔隙水压力的变化规律。结果表明,降雨强度越大,空隙水压力上升越快;相同降雨量条件下,降雨强度越小,空隙水压... 为更准确地分析降雨条件下边坡的稳定性,以大连变电站拟开挖边坡为实例,运用SEEP/W分析了不同持时和降雨强度条件下边坡内部孔隙水压力的变化规律。结果表明,降雨强度越大,空隙水压力上升越快;相同降雨量条件下,降雨强度越小,空隙水压力上升越大。随后将SEEP/W所得结果耦合到SLOPE/W中进行稳定性计算,计算结果表明降雨导致边坡安全系数下降;相同降雨量条件下,持时越长,安全系数越低。相应结果为利用SEEP/W和SLOPE/W耦合分析解决降雨条件下边坡稳定性问题提供了参考案例。 展开更多
关键词 seep/W SLOPE/W 降雨入渗 稳定性分析 边坡工程
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基于SEEPS方法的重庆地区降水数值预报性能分析 被引量:1
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作者 陈良吕 陈法敬 夏宇 《西南大学学报(自然科学版)》 CAS CSCD 北大核心 2019年第7期116-124,共9页
本研究简要介绍了SEEPS方法的具体计算方案,将该方法应用到重庆地区的降水数值预报检验中,对重庆地区常用的3个业务数值模式2017年全年的预报结果进行了检验评估,并对比分析了3个模式降水预报性能的总体差异及时空分布特征.结果表明,综... 本研究简要介绍了SEEPS方法的具体计算方案,将该方法应用到重庆地区的降水数值预报检验中,对重庆地区常用的3个业务数值模式2017年全年的预报结果进行了检验评估,并对比分析了3个模式降水预报性能的总体差异及时空分布特征.结果表明,综合各个预报时效2017年全年区域平均SEEPS技巧评分的结果, EC模式的降水预报性能最优,其次是SWC-WARMS, CQMFS最差;综合各个预报时效2017年1-12月逐月区域平均的SEEPS技巧评分的结果, SWC-WARMS各月的预报性能均优于CQMFS. SWC-WARMS和CQMFS的降水预报性能在7月和8月总体而言优于EC模式,其余各月均差于EC模式;对于同一区域全年平均的降水数值预报性能, EC模式最优,其次是SWC-WARMS, CQMFS最差.各个模式的SEEPS技巧评分在四川盆地东部偏东地区均存在大值中心. EC模式总体表现出在重庆的东北部偏东地区和中西部偏北地区的SEEPS技巧评分优于重庆的其他地区. SWC-WARMS总体表现出在重庆东南部地区的SEEPS技巧评分优于重庆的其他地区. CQMFS总体表现出在重庆的东南部地区和重庆的中西部偏北地区的SEEPS技巧评分优于其他地区. 展开更多
关键词 降水预报 检验方法 seepS方法 概率空间
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基于SEEP/W程序的堤防截渗墙防渗性能研究 被引量:3
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作者 杨扬 徐伟 +1 位作者 李再兴 孙健 《黄河水利职业技术学院学报》 2016年第1期1-5,共5页
以沁河河道现状堤防为研究对象,通过对工程地质条件的分析,建立地质概化模型,采用地下水渗流程序Geo Studio-Seep/W建立二维有限元模型,分析截渗墙的深度和厚度对防渗性能的影响。研究结果表明:截渗墙明显降低了地下水的浸润面,增加了... 以沁河河道现状堤防为研究对象,通过对工程地质条件的分析,建立地质概化模型,采用地下水渗流程序Geo Studio-Seep/W建立二维有限元模型,分析截渗墙的深度和厚度对防渗性能的影响。研究结果表明:截渗墙明显降低了地下水的浸润面,增加了非饱和土的范围,同时减少了高水头的范围,对大堤和堤基土的稳定性有利;截渗墙对断面流量、出逸高度和出逸坡降都有明显的降低,而截渗墙厚度对以上因素的影响不大;全截式截渗墙的防渗效果最好,改变全截式截渗墙的深度,防渗效果没有改变,截渗墙厚度为20 cm、深入相对隔水层1~2 m时,可满足防渗要求。 展开更多
关键词 沁河 堤防 截渗墙 防渗性能 seep/W 有限元分析
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基于SEEP/W的基底地下水赋存形态及孔压分析 被引量:3
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作者 雷波 明世祥 贾立宏 《中南公路工程》 2005年第4期99-103,共5页
依托SEEP/W软件和实测数据模拟地下水压力在某工程基底及侧墙的分布情况,分析在抗浮水位下的渗流物理过程,确定其基底水压力及分布规律。
关键词 seep/W 渗流模拟 基底水压力 抗浮水位
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Microbial diversity in cold seep sediments from the northern South China Sea 被引量:11
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作者 Yong Zhang Xin Su +5 位作者 Fang Chen Yuanyuan Wang Lu Jiao Hailiang Dong Yongyang Huang Hongchen Jiang 《Geoscience Frontiers》 SCIE CAS 2012年第3期301-316,共16页
South China Sea (SCS) is the largest Western Pacific marginal sea. However, microbial studies have never been performed in the cold seep sediments in the SCS. In 2004, "SONNE" 177 cruise found two cold seep areas ... South China Sea (SCS) is the largest Western Pacific marginal sea. However, microbial studies have never been performed in the cold seep sediments in the SCS. In 2004, "SONNE" 177 cruise found two cold seep areas with different water depth in the northern SCS. Haiyang 4 area, where the water depth is around 3000 m, has already been confirmed for active seeping on the seafloor, such as microbial mats, authigenic carbonate crusts and bivalves. We investigated microbial abundance and diver- sity in a 5.55-m sediment core collected from this cold seep area. An integrated approach was employed including geochemistry and 16S rRNA gene phylogenetic analyses. Here, we show that microbial abun- dance and diversity along with geochemistry profiles of the sediment core revealed a coupled reaction between sulphate reduction and methane oxidation. Acridine orange direct count results showed that microbial abundance ranges from 105 to 106 cells/g sediment (wet weight). The depth-related variation of the abundance showed the same trend as the methane concentration profile. Phylogenetic analysis indicated the presence of sulphate-reducing bacteria and anaerobic methane-oxidizing archaea. The diver- sity was much higher at the surface, but decreased sharply with depth in response to changes in the geochemical conditions of the sediments, such as methane, sulphate concentration and total organic carbon. Marine Benthic Group B, Chloroflexi and JS1 were predominant phylotypes of the archaeal and bacterial libraries, respectively. 展开更多
关键词 16S rRNA Microbial diversity Cold seep Marine sediments Northern South China Sea
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An examination by GC×GC-TOFMS of organic molecules present inhighly degraded oils emerging from Caribbean terrestrial seeps of Cretaceous age 被引量:5
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作者 Alan G.Scarlett Ana Ibis Despaigne-Diaz +1 位作者 Simon A.Wilde Kliti Grice 《Geoscience Frontiers》 SCIE CAS CSCD 2019年第1期5-15,共11页
For our ancestors, oil seeps were both a fascination and a resource but as the planet's reserves of high quality low density oil becomes increasingly depleted, so there is now a renewed interest in heavier,biodegr... For our ancestors, oil seeps were both a fascination and a resource but as the planet's reserves of high quality low density oil becomes increasingly depleted, so there is now a renewed interest in heavier,biodegraded oils such as those encountered in terrestrial seeps. One such seep is Pitch Lake in the Caribbean island of Trinidad, which is the largest natural deposit of asphalt in the world. At the northern end of the Caribbean, oil emerges along a tectonic contact on the island on Cuba. The sources of the oils from these seeps are relatively recent and both are subject to intense weathering due to the tropical conditions. When analysed by gas chromatography(GC) both oils appear as unresolved complex mixtures(UCM) and show a very high degree of biodegradation thus presenting an analytical challenge. In this case study, these two Caribbean seep oils were analysed by comprehensive two dimensional GC with time of flight mass spectrometry(GC×GC-TOFMS) to expose many thousands of the individual compounds that comprise the UCM. The high chromatographic resolution of the GC×GC-TOFMS produced good quality mass spectra allowing many compounds including molecular fossil ‘biomarkers' to be identified. Compound classes included diamondoid hydrocarbons, demethylated hopanes and secohopanes, mono-and tri-aromatic steroids. D-ring aromatised structures of the 8,14-seco-hopanes,including demethylated forms were present in both oils but further demethylation, probably at position C-25 during biodegradation, was only observed in the Pitch Lake oil. Many polycyclic aromatic hydrocarbons(PAHs) were absent although the fungal-derived pentacyclic PAH perylene was present in both oils. The presence of the angiosperm biomarker lupane in the Pitch Lake oil constrained the age to the Late Cretaceous. The higher degree of biodegradation observed in the Cuban oil was likely due to relatively slow anaerobic processes whereas oil within Pitch Lake was probably subject to additional more rapid aerobic metabolism within the lake. 展开更多
关键词 Oil seep Biomarker Biodegradation GCxGC DIAMONDOIDS AROMATIC STEROIDS
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