Objective:To evaluate the antibacterial activity of foliar extracts of Rhizophora mucronnata(R. mucronata) against pathogens belonging to human origin and to identify the compound hitherto unprecedented in nature by G...Objective:To evaluate the antibacterial activity of foliar extracts of Rhizophora mucronnata(R. mucronata) against pathogens belonging to human origin and to identify the compound hitherto unprecedented in nature by GC-MS analysis.Methods:Soxhlet extraction method was used to get the corresponding extracts of ethanol,petroleum ether,acetone,methanol and ethyl acetate.The antimicrobial activities of the organic solvent extracts on the various test organisms using agar well diffusion technique were carried out.Ethyl acetate extract exhibited promising antimicrobial activity and hence minimum inhibitory concentration(MIC) was performed for the same.Column chromatography was done for partial purification of crude extract and fractions were analyzed by GC-MS.Results:A column chromatographic fractionation of the extracts and further UV visible and GS-MS analysis suggested the active principle compound were a mixture of squalene(19.19%),n-Hexadecanoic acid(6.59%),phytol(4.74%).2-cyclohexane-l- one, 4-hydroxy-3,5,(4.20%) and oleic acid(2.88%).Conclusions:The results are good enough to serve to transform the practice of research in this sub field across a range of different benefit streams that include drug development.By and large this type of structure analyses are most important as aids to more rational decision taking in safety models versus effectiveness.In general,structural data provide prima facie support for drug hypothesis.展开更多
[ Objective] A method was developed for the analysis of main metabolites in aromatic rice seeds by gas chromatography-mass spectrometry （GC-MS）. [ Method] The main metabolites in seeds of 9 different aromatic rice v...[ Objective] A method was developed for the analysis of main metabolites in aromatic rice seeds by gas chromatography-mass spectrometry （GC-MS）. [ Method] The main metabolites in seeds of 9 different aromatic rice varieties were compared and analyzed with two methods. Method 1 ： the compound was extracted by the mixture of anhydrous ethanol and methylene chloride （ WV = 1：1 ） at 60 ℃ for 3 h in water bath; the extract was isolated and detected by gas chromatography-mass spectrometry using HP-5 capillary column with scan monitoring mode. Method 2： the main metabolites in aromatic rice seeds were detected using headspaee solid phase micro-extraction and gas chromatography mass spectrometry （GC-MS）. [ Result ] Fifteen types of common metabolites were found in 9 different aromatic rice varieties. Method 1 was more effective than method 2 in detecting the main metabolites in aromatic rice seeds. [ Conclusion] This study provides an important reference for clarifying the metabolic pathway of main metabolites in aromatic rice and lays the foundation for further rice breeding.展开更多
An analytical method was developed to quantitatively determine selected monohydroxy metabolites of polycyclic aromatic hydrocarbons(PAHs) in human urine. The procedure included enzymatic hydrolysis to cleave the conju...An analytical method was developed to quantitatively determine selected monohydroxy metabolites of polycyclic aromatic hydrocarbons(PAHs) in human urine. The procedure included enzymatic hydrolysis to cleave the conjugated metabolites, solid-phase microextraction enrichment, and gas chromatography-mass spectrometry analysis. The method proved to be sensitive enough to detect the selected PAH metabolites in human urine.展开更多
Soybean[Glycine max（L.） Merr.]is one of the world＇s major crops,and soybean seeds are a rich and important resource for proteins and oils.While ＂omics＂ studies,such as genomics,transcriptomics,and proteomics,have...Soybean[Glycine max（L.） Merr.]is one of the world＇s major crops,and soybean seeds are a rich and important resource for proteins and oils.While ＂omics＂ studies,such as genomics,transcriptomics,and proteomics,have been widely applied in soybean molecular research,fewer metabolomic studies have been conducted for largescale detection of low molecular weight metabolites,especially in soybean seeds.In this study,we investigated the seed metabolomes of 29 common soybean cultivars through combined gas chromatography-mass spectrometry and ultra-performance liquid chromatography-tandem mass spectrometry.One hundred sixty-nine named metabolites were identified and subsequently used to construct a metabolic network of mature soybean seed.Among the 169 detected metabolites,104 were found to be significantly variable in their levels across tested cultivars.Metabolite markers that could be used to distinguish genetically related soybean cultivars were also identified,and metabolitemetabolite correlation analysis revealed some significant associations within the same or among different metabolite groups.Findings from this work may potentially provide the basis for further studies on both soybean seed metabolism and metabolic engineering to improve soybean seed quality and yield.展开更多
Growth and health responses of pigs fed fermented liquid diet are not always consistent and causes for this issue are still not very clear. Metabolites produced at different fermentation time points should be one of t...Growth and health responses of pigs fed fermented liquid diet are not always consistent and causes for this issue are still not very clear. Metabolites produced at different fermentation time points should be one of the most important contributors. However, currently no literatures about differential metabolites of fermented liquid diet are reported. The aim of this experiment was to explore the difference of metabolites in a fermented liquid diet between different fermentation time intervals. A total of eighteen samples that collected from Bacillus subtilis fermented liquid diet on days 7, 21 and 35 respectively were used for the identification of metabolites by gas chromatography time of flight mass spectrometry(GCTOF-MS). Fifteen differential metabolites including melibiose, sortitol, ribose, cellobiose, maltotriose,sorbose, iso maltose, maltose, fructose, D-glycerol-1-phosphate, 4-aminobutyric acid, beta-alanine, tyrosine, pyruvic acid and pantothenic acid were identified between 7-d samples and 21-d samples. The relative level of melibiose, ribose, maltotriose, D-glycerol-1-phosphate, tyrosine and pyruvic acid in samples collected on day 21 was significantly higher than that in samples collected on day 7(P < 0.01),respectively. Eight differential metabolites including ribose, sorbose, galactinol, cellobiose, pyruvic acid,galactonic acid, pantothenic acid and guanosine were found between 21-d samples and 35-d samples.Samples collected on day 35 had a higher relative level of ribose than that in samples collected on day 21(P < 0.01), In conclusion, many differential metabolites which have important effects on the growth and health of pigs are identified and findings contribute to explain the difference in feeding response of fermented liquid diet.展开更多
文摘Objective:To evaluate the antibacterial activity of foliar extracts of Rhizophora mucronnata(R. mucronata) against pathogens belonging to human origin and to identify the compound hitherto unprecedented in nature by GC-MS analysis.Methods:Soxhlet extraction method was used to get the corresponding extracts of ethanol,petroleum ether,acetone,methanol and ethyl acetate.The antimicrobial activities of the organic solvent extracts on the various test organisms using agar well diffusion technique were carried out.Ethyl acetate extract exhibited promising antimicrobial activity and hence minimum inhibitory concentration(MIC) was performed for the same.Column chromatography was done for partial purification of crude extract and fractions were analyzed by GC-MS.Results:A column chromatographic fractionation of the extracts and further UV visible and GS-MS analysis suggested the active principle compound were a mixture of squalene(19.19%),n-Hexadecanoic acid(6.59%),phytol(4.74%).2-cyclohexane-l- one, 4-hydroxy-3,5,(4.20%) and oleic acid(2.88%).Conclusions:The results are good enough to serve to transform the practice of research in this sub field across a range of different benefit streams that include drug development.By and large this type of structure analyses are most important as aids to more rational decision taking in safety models versus effectiveness.In general,structural data provide prima facie support for drug hypothesis.
基金Supported by National Natural Science Foundation of China(U1604110,31600992,U1404319,31270727)Key Science and Technology Program of Henan Province(152102110100,152102110036)+6 种基金Major Project of Science and Technology of Henan Province(121100110200)Project of Scientific and Technological Innovation Team for Colleges and Universities in Henan Province(14IRTSTHN012)Students Innovation and Training Program of Institution of Higher Learning in Henan Province(201710477018Y)Key Research Projects of Henan Higher Education Institutions(15A180059,16B180006)Students Innovation and Training Program of XYNU(201710477032)Nanhu Scholars Program for Young Scholars of XYNY(2016054)Open Project of the Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains
文摘[ Objective] A method was developed for the analysis of main metabolites in aromatic rice seeds by gas chromatography-mass spectrometry （GC-MS）. [ Method] The main metabolites in seeds of 9 different aromatic rice varieties were compared and analyzed with two methods. Method 1 ： the compound was extracted by the mixture of anhydrous ethanol and methylene chloride （ WV = 1：1 ） at 60 ℃ for 3 h in water bath; the extract was isolated and detected by gas chromatography-mass spectrometry using HP-5 capillary column with scan monitoring mode. Method 2： the main metabolites in aromatic rice seeds were detected using headspaee solid phase micro-extraction and gas chromatography mass spectrometry （GC-MS）. [ Result ] Fifteen types of common metabolites were found in 9 different aromatic rice varieties. Method 1 was more effective than method 2 in detecting the main metabolites in aromatic rice seeds. [ Conclusion] This study provides an important reference for clarifying the metabolic pathway of main metabolites in aromatic rice and lays the foundation for further rice breeding.
基金supported by the National Natural Science Foundation of China(21227006,21275088)the China Equipment and Education Resources System(CERS-1-75)
文摘An analytical method was developed to quantitatively determine selected monohydroxy metabolites of polycyclic aromatic hydrocarbons(PAHs) in human urine. The procedure included enzymatic hydrolysis to cleave the conjugated metabolites, solid-phase microextraction enrichment, and gas chromatography-mass spectrometry analysis. The method proved to be sensitive enough to detect the selected PAH metabolites in human urine.
基金supported by the China National Transgenic Plant Special Fund(2011ZX08012-002 and 2013ZX08012-002)Innovative Research Team Fund(Ministry of Education,China)111 Project Fund(B14016)
文摘Soybean[Glycine max（L.） Merr.]is one of the world＇s major crops,and soybean seeds are a rich and important resource for proteins and oils.While ＂omics＂ studies,such as genomics,transcriptomics,and proteomics,have been widely applied in soybean molecular research,fewer metabolomic studies have been conducted for largescale detection of low molecular weight metabolites,especially in soybean seeds.In this study,we investigated the seed metabolomes of 29 common soybean cultivars through combined gas chromatography-mass spectrometry and ultra-performance liquid chromatography-tandem mass spectrometry.One hundred sixty-nine named metabolites were identified and subsequently used to construct a metabolic network of mature soybean seed.Among the 169 detected metabolites,104 were found to be significantly variable in their levels across tested cultivars.Metabolite markers that could be used to distinguish genetically related soybean cultivars were also identified,and metabolitemetabolite correlation analysis revealed some significant associations within the same or among different metabolite groups.Findings from this work may potentially provide the basis for further studies on both soybean seed metabolism and metabolic engineering to improve soybean seed quality and yield.
基金supported by Jiangxi Provincial Key Technology R&D Program (20121BBF60032 and 20132BBF60039)
文摘Growth and health responses of pigs fed fermented liquid diet are not always consistent and causes for this issue are still not very clear. Metabolites produced at different fermentation time points should be one of the most important contributors. However, currently no literatures about differential metabolites of fermented liquid diet are reported. The aim of this experiment was to explore the difference of metabolites in a fermented liquid diet between different fermentation time intervals. A total of eighteen samples that collected from Bacillus subtilis fermented liquid diet on days 7, 21 and 35 respectively were used for the identification of metabolites by gas chromatography time of flight mass spectrometry(GCTOF-MS). Fifteen differential metabolites including melibiose, sortitol, ribose, cellobiose, maltotriose,sorbose, iso maltose, maltose, fructose, D-glycerol-1-phosphate, 4-aminobutyric acid, beta-alanine, tyrosine, pyruvic acid and pantothenic acid were identified between 7-d samples and 21-d samples. The relative level of melibiose, ribose, maltotriose, D-glycerol-1-phosphate, tyrosine and pyruvic acid in samples collected on day 21 was significantly higher than that in samples collected on day 7(P < 0.01),respectively. Eight differential metabolites including ribose, sorbose, galactinol, cellobiose, pyruvic acid,galactonic acid, pantothenic acid and guanosine were found between 21-d samples and 35-d samples.Samples collected on day 35 had a higher relative level of ribose than that in samples collected on day 21(P < 0.01), In conclusion, many differential metabolites which have important effects on the growth and health of pigs are identified and findings contribute to explain the difference in feeding response of fermented liquid diet.
