Emulsion and polyphase liposome (PL) of ricinoleic acid (RA) and RA containing selenium (Se-RA) were prepared by tween-80 treatment and injection-ultrasonic method. Their antitumor effects were tested in vitro and in ...Emulsion and polyphase liposome (PL) of ricinoleic acid (RA) and RA containing selenium (Se-RA) were prepared by tween-80 treatment and injection-ultrasonic method. Their antitumor effects were tested in vitro and in vivo. As a result, the emulsion of RA (91 ) and Sc-RA (92) can completely kill the mouse ascites tumor cells of S180 in vitro (500 μg/ml). While. 97% cells of the control group still survive. When tested in vivo, 91 and 92 were injected into mice peritoneally (200 mg/Kg/day) for 7 days, their inhibit rate on S180 are 30. 6% (P<0. 05) and 33. 3% (P<0. 05) respectively. For the PL of RA (113) and Se-RA (114), the inhibit rate are 58% (P< 0.001) and 61.4% (P<0. 001) respectively (400 mg/kg/day). The result of 3H-TdR incorporation experiment indicates that the possible antitumor mechanism of Se-Ra is that it can inhibit the incorporation of 3H- TdR into tumor cells and hense inhibit the DNA synthesis of the tumor cells.展开更多
In recent years, close attention has been paid to the study of liposomes as the carrier of the anticancerous drug, which has many advantages in the therapeutics, such as increasing the toxicity of the drug. alleviatin...In recent years, close attention has been paid to the study of liposomes as the carrier of the anticancerous drug, which has many advantages in the therapeutics, such as increasing the toxicity of the drug. alleviating metamorphosis and immunity reaction; improving the biological utilization ratio of the drug; selectively killing and inhibiting the cancer cells or checking the reproduction of the cancer cells, and so on. At present, peo-展开更多
文摘Emulsion and polyphase liposome (PL) of ricinoleic acid (RA) and RA containing selenium (Se-RA) were prepared by tween-80 treatment and injection-ultrasonic method. Their antitumor effects were tested in vitro and in vivo. As a result, the emulsion of RA (91 ) and Sc-RA (92) can completely kill the mouse ascites tumor cells of S180 in vitro (500 μg/ml). While. 97% cells of the control group still survive. When tested in vivo, 91 and 92 were injected into mice peritoneally (200 mg/Kg/day) for 7 days, their inhibit rate on S180 are 30. 6% (P<0. 05) and 33. 3% (P<0. 05) respectively. For the PL of RA (113) and Se-RA (114), the inhibit rate are 58% (P< 0.001) and 61.4% (P<0. 001) respectively (400 mg/kg/day). The result of 3H-TdR incorporation experiment indicates that the possible antitumor mechanism of Se-Ra is that it can inhibit the incorporation of 3H- TdR into tumor cells and hense inhibit the DNA synthesis of the tumor cells.
文摘In recent years, close attention has been paid to the study of liposomes as the carrier of the anticancerous drug, which has many advantages in the therapeutics, such as increasing the toxicity of the drug. alleviating metamorphosis and immunity reaction; improving the biological utilization ratio of the drug; selectively killing and inhibiting the cancer cells or checking the reproduction of the cancer cells, and so on. At present, peo-
