Selenium nanoparticles(SeNPs)have been demonstrated potential for use in diseases associated with oxidative stress.Functionalized SeNPs with lower toxicity and higher biocompatibility could bring better therapeutic ac...Selenium nanoparticles(SeNPs)have been demonstrated potential for use in diseases associated with oxidative stress.Functionalized SeNPs with lower toxicity and higher biocompatibility could bring better therapeutic activity and clinical application value.Herein,this work was conducted to investigate the protective effect of Pleurotus tuber-regium polysaccharide-protein complex funtionnalized SeNPs(PTR-SeNPs)against acetaminophen(APAP)-induced oxidative injure in HepG2 cells and C57BL/6J mouse liver.Further elucidation of the underlying molecular mechanism,in particular their modulation of Nrf2 signaling pathway was also performed.The results showed that PTR-SeNPs could significantly ameliorate APAP-induced oxidative injury as evidenced by a range of biochemical analysis,histopathological examination and immunoblotting study.PTR-SeNPs could hosphorylate and activate PKCδ,depress Keap1,and increase nuclear accumulation of Nrf2,resulting in upregulation of GCLC,GCLM,HO-1 and NQO-1 expression.Besides,PTR-SeNPs suppressed the biotransformation of APAP to generate intracellular ROS through CYP 2E1 inhibition,restoring the mitochondrial morphology.Furthermore,the protective effect of PTR-SeNPs against APAP induced hepatotoxicity was weakened as Nrf2 was depleted in vivo,indicating the pivotal role of Nrf2 signaling pathway in PTR-SeNPs mediated hepatoprotective efficacy.Being a potential hepatic protectant,PTR-SeNPs could serve as a new source of selenium supplement for health-promoting and biomedical applications.展开更多
Nowadays,doctors and nutritionists recommend individuals incorporate selenium-rich foods such as nuts,cereals,and mushrooms into their regular diet to maintain fitness and overall health.Selenium nanoparticles(SeNPs)e...Nowadays,doctors and nutritionists recommend individuals incorporate selenium-rich foods such as nuts,cereals,and mushrooms into their regular diet to maintain fitness and overall health.Selenium nanoparticles(SeNPs)exhibit strong chemopreventive capabilities.The anticipations for SeNPs with enhanced and tunable bioactive activities have led to a keen interest in phytofabrication.In this study,the aqueous extract of Clerodendron phlomidis plant leaves was utilized for the synthesis of SeNPs.In traditional Indian medicine,this plant extract is recognized as a significant anti-diabetic agent.The flavonoids tetrahydroxylflavone,7-hydroxyflavanone,and 6,4’-dimethyl-7-acetoxy-scutellarein present in this plant leaf extract demonstrate excellent anticancer activity.These secondary metabolites exhibit the ability to reduce sodium selenite into SeNPs.At a concentration of 13μg/mL,the synthesized SeNPs effectively inhibited the proliferation of the HepG2 cell line.The results suggest that the SeNPs possess promising anti-cancer potential against liver cancer and can be considered as a therapeutic agent for liver cancer treatment.Additionally,the cell cycle arrest induced by SeNPs was further confirmed by the fluorescence-activated cell sorting(FACS)method,indicating that SeNPs could efficiently differentiate cancer cells from normal cells.Notably,it showed a significant improvement in diethylnitrosamine(DEN)-induced Swiss Wistar rat groups.This scientific investigation highlights the high anti-cancer potential of SeNPs,positioning them as a promising therapeutic agent for liver cancer treatment.展开更多
AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activit...AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress.A safe and effective nano-drug system of functionalized selenium-containing nanoparticles(SeNPs)was developed with lentinan(LNT)(SeNPs@LNT).Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT(1,2,4,8,16,32μM)for 24-72 h to evaluate the cytotoxicity of selenium.GC2-spg cells were randomly divided into the following groups:control,hydrogen peroxide(H_(2)O_(2)),SeNPs@LNT,and H_(2)O_(2)+SeNPs@LNT groups.H_(2)O_(2)+SeNPs@LNT group was pretreated with SeNPs@LNT 4μM for 12 h,followed by H_(2)O_(2)600μM for 8 h.The cell viability decreased in the H_(2)O_(2) group and increased significantly in the SeNPs@LNT group.Compared with the H_(2)O_(2) group,the SeNPs@LNT+H_(2)O_(2) group exhibited obvious red fluorescence,indicating a higher level of mitochondrial membrane potential.The content of intracellular reactive oxygen species(ROS)in the SeNPs@LNT group reduced significantly,and the intensity of green fluorescence in the SeNPs@LNT+H_(2)O_(2) group decreased significantly compared with the H_(2)O_(2) group,indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress.The activity of GPx and SOD increased significantly in the SeNPs@LNT group.The expression of p53 decreased significantly under the intervention of nano-selenium,and GPx1 expression increased.In the oxidative stress group,the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups.Thus,SeNPs@LNT can promote GC2-spg cell proliferation,improve GPx and SOD activities,remove intracellular ROS,and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels.SeNPs@LNT has good biological activity and antioxidant effect,which can be used to protect the male reproductive system from oxidative stress.展开更多
Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to invest...Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to investigate its antioxidant effect.This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide(H_(2)O_(2))-induced Leydig cell line,TM3.Methods:The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed.The concentration of 4μM could significantly promote the growth of TM3 cells.Oxidative stress damage was caused using an 800μM concentration of hydrogen peroxide.The cells were divided into four groups:normal control group,oxidative stress treatment group,H_(2)O_(2)+SeNPs@LNT group,and SeNPs@LNT group.The H_(2)O_(2)+SeNPs@LNT group was pretreated with 4μM of SeNPs@LNT for 12 h,followed by 800μM of H_(2)O_(2)for 8 h.Results:Nano-selenium could significantly promote the proliferation and viability of TM3 cells.SeNPs@LNT treatment increased the level of mitochondrial membrane potential in normal cells and slowed down the decline in mitochondrial membrane potential level caused by oxidative stress injury.In addition,the increase in reactive oxygen species caused by oxidative stress was inhibited by SeNPs@LNT treatment.The apoptosis of TM3 cells was detected,and SeNPs@LNT alleviated the necrosis and apoptosis of TM3 cells induced by H_(2)O_(2).Nano-selenium plays a protective role against oxidative H_(2)O_(2)-induced stress injury in TM3 cells through the changes in the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and P53 pathway,and the expression levels of other related proteins,protein kinase B(AKT)and C3.Conclusion:SeNPs@LNT exhibited good biological activity and antioxidant effect and can thus be used to protect the male reproductive system from oxidative stress.展开更多
In the present study,we investigated the synergistic effects of targeted methotrexate-selenium nanostructure containing Myc decoy oligodeoxynucleotides along with X-irradiation exposure as a combination therapy on LNC...In the present study,we investigated the synergistic effects of targeted methotrexate-selenium nanostructure containing Myc decoy oligodeoxynucleotides along with X-irradiation exposure as a combination therapy on LNCaP prostate cancer cells.Myc decoy ODNs were designed based on the promoter of Bcl-2 gene and analyzed by molecular docking and molecular dynamics assays.ODNs were loaded on the synthesized Se@BSA@Chi-MTX nanostructure.The physicochemical characteristics of nanostructures were determined by FTIR,DLS,UV-vis,TEM,EDX,in vitro release,and hemolysis tests.Subsequently,the cytotoxicity properties of them with and without X-irradiation were investigated by uptake,MTT,cell cycle,apoptosis,and scratch assays on the LNCaP cell line.The results of DLS and TEM showed negative charge(−9 mV)and nanometer size(40 nm)for Se@BSA@Chi-DEC-MTX NPs,respectively.The results of FTIR,UV-vis,and EDX showed the proper interaction of different parts and the correct synthesis of nanoparticles.The results of hemolysis showed the hemocompatibility of this nanoparticle in concentrations less than 6 mg/mL.The ODNs release from the nanostructures showed a pH-dependent manner,and the release rate was 15%higher in acidic pH.The targeted Se@BSA@Chi-labeled ODN-MTX NPs were efficiently taken up by LNCaP cells by targeting the prostate-specific membrane antigen(PSMA).The significant synergistic effects of nanostructure(containing MTX drug)treatment along with X-irradiation showed cell growth inhibition,apoptosis induction(~57%),cell cycle arrest(G2/M phase),and migration inhibition(up to 90%)compared to the control.The results suggested that the Se@BSA@Chi-DEC-MTX NPs can potentially suppress the cell growth of LNCaP cells.This nanostructure system can be a promising approach for targeted drug delivery and chemoradiotherapy in prostate cancer treatment.展开更多
The well-distributed, stable selenium nanoparticles (10 nm) with good adhesive ability and biocompatibility were successfully synthesized by using the template of chitosan cross-linked with glutaradehyde. The resulti...The well-distributed, stable selenium nanoparticles (10 nm) with good adhesive ability and biocompatibility were successfully synthesized by using the template of chitosan cross-linked with glutaradehyde. The resulting selenium nanoparticles were used as a new carrier for horseradish peroxidase to construct H2O2 biosensors with good performances.展开更多
<span style="font-family:Verdana;">A simple method for assessment of the toxicity and antidote effect of selenium nanoparticles with </span><i><i><span style="font-family:Verd...<span style="font-family:Verdana;">A simple method for assessment of the toxicity and antidote effect of selenium nanoparticles with </span><i><i><span style="font-family:Verdana;">Paramecium</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;"> is presented. Light microscopy in combination with computerized video tracking is employed for </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">determination of </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">survival time of <i></i></span><i><i><span style="font-family:Verdana;">P.</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i></span><span style="font-family:Verdana;">. Up to 800 mg/L, selenium nanoparticles are not acutely toxic. </span><span style="font-family:Verdana;">With</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> respect to a potential antidote effect, the lethality of silver nanoparticles, silver nitrate, sodium hydrogen selenite, and sodium selenite to <i></i></span><i><i><span style="font-family:Verdana;">P.</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i></span><span style="font-family:Verdana;"> was decreased and survival time was extend</span><span style="font-family:Verdana;">ed upon pre-treatment with selenium nanoparticles. Taken together, these findings suggest that administration of selenium nanoparticles attenuates</span><span style="font-family:Verdana;"> exposure </span><span style="font-family:Verdana;">to</span><span style="font-family:Verdana;"> toxicants. Selenium nanoparticles could be a good functional additive for food management in animals.</span>展开更多
The association of gut microflora and human health is being increasingly recognized,and the impact of gut microflora on the host is well characterized,including the body’s energy metabolism and immune system maintena...The association of gut microflora and human health is being increasingly recognized,and the impact of gut microflora on the host is well characterized,including the body’s energy metabolism and immune system maintenance.Several human diseases,including metabolic,autoimmune,obesity,hypothyroidism,and intestinal disorders,are closely associated with gut dysbiosis.Inorganic nanoparticles(NPs)are extensively utilized in numerous fields due to their distinctive,attractive physicochemical properties.Estimation of the potential impacts of NPs,with a high number of microorganisms inside the human body(microbiota)and its genomes(microbiome),represents one of the most important aspects of nano-toxicology.This review article aims to provide information on the association of gut microflora alterations to diseases and describe the impacts of various inorganic NPs,including silver,zinc,selenium,titania,silicon,and copper,on gut microflora.Research on the effect of inorganic NPs on gut microflora of animal models and the poultry industry is reviewed.The response of pathogenic Enterobacter species to inorganic NPs has been expounded in detail.This review also highlights the need to focus on the ancillary effects of various inorganic NPs on gut microflora to expedite the suitable advancement of these particles for future use.Finally,the key opportunistic areas for the application of nanotechnology are underlined to manipulate the microbiome of gut dysbiosis,provide an overview,and address potential challenges and our perspective on this evolving field.展开更多
The productivity and sustainability of livestock production systems are heavily influenced by animal nutrition.To maintain homeostatic balance in the body of the animal at different phases of life,the percentage of or...The productivity and sustainability of livestock production systems are heavily influenced by animal nutrition.To maintain homeostatic balance in the body of the animal at different phases of life,the percentage of organically active minerals in livestock feed must be optimized.Selenium(Se)is a crucial trace mineral that is required for the maintenance of many functions of the body.Se nanoparticles(SeNPs)attracted considerable interest from researchers for a variety of applications a decade ago,owing to their extraordinary properties.SeNPs offer significant advantages over larger-sized materials,by having a comparatively wider surface area,increased surface energy,and high volume.Despite its benefits,SeNP also has toxic effects,therefore safety concerns must be taken for a successful application.The toxicological effects of SeNPs in animals are characterized by weight loss,and increased mortality rate.A safe-by-strategy to certify animal,human and environmental safety will contribute to an early diagnosis of all risks associated with SeNPs.This review is aimed at describing the beneficial uses and potential toxicity of SeNPs in various animals.It will also serve as a summary of different levels of SeNPs which should be added in the feed of animals for better performance.展开更多
Selenium nanoparticles(SeNPs)are proposed as a safer and more effective selenium delivery system than sodium selenite(Na_(2)SeO_(3)).Here,we investigated the effects of replacing dietary Na_(2)SeO_(3)with SeNPs synthe...Selenium nanoparticles(SeNPs)are proposed as a safer and more effective selenium delivery system than sodium selenite(Na_(2)SeO_(3)).Here,we investigated the effects of replacing dietary Na_(2)SeO_(3)with SeNPs synthesized by Lactobacillus casei ATCC 393 on the growth performance and gut health of earlyweaned piglets.Seventy-two piglets(Duroc×Landrace×Large Yorkshire)weaned at 21 d of age were divided into the control group(basal diet containing 0.3 mg Se/kg from Na_(2)SeO_(3))and SeNPs group(basal diet containing 0.3 mg Se/kg from SeNPs)during a 14-d feeding period.The results revealed that SeNPs supplementation increased the average daily gain(P=0.022)and average daily feed intake(P=0.033),reduced(P=0.056)the diarrhea incidence,and improved(P=0.013)the feed conversion ratio compared with Na_(2)SeO_(3).Additionally,SeNPs increased jejunal microvilli height(P=0.006)and alleviated the intestinal barrier dysfunction by upregulating(P<0.05)the expression levels of mucin 2 and tight junction proteins,increasing(P<0.05)Se availability,and maintaining mitochondrial structure and function,thereby improving antioxidant capacity and immunity.Furthermore,metabolomics showed that SeNPs can regulate lipid metabolism and participate in the synthesis,secretion and action of parathyroid hormone,proximal tubule bicarbonate reclamation and tricarboxylic acid cycle.Moreover,SeNPs increased(P<0.05)the abundance of Holdemanella and the levels of acetate and propionate.Correlation analysis suggested that Holdemanella was closely associated with the regulatory effects of SeNPs on early-weaned piglets through participating in lipid metabolism.Overall,replacing dietary Na_(2)SeO_(3)with biogenic SeNPs could be a potential nutritional intervention strategy to prevent earlyweaning syndrome in piglets.展开更多
基金financially supported by National Natural Science Foundation of China(81700524)Natural Science Foundation of Fujian Province(2022J01866)from Fujian Provincial Department of Science and Technology+1 种基金Key Project of Fujian University of Traditional Chinese Medicine(X2021019)Collaborative Innovation and Platform Establishment Project of Department of Science and Technology of Guangdong Province(2019A050520003)。
文摘Selenium nanoparticles(SeNPs)have been demonstrated potential for use in diseases associated with oxidative stress.Functionalized SeNPs with lower toxicity and higher biocompatibility could bring better therapeutic activity and clinical application value.Herein,this work was conducted to investigate the protective effect of Pleurotus tuber-regium polysaccharide-protein complex funtionnalized SeNPs(PTR-SeNPs)against acetaminophen(APAP)-induced oxidative injure in HepG2 cells and C57BL/6J mouse liver.Further elucidation of the underlying molecular mechanism,in particular their modulation of Nrf2 signaling pathway was also performed.The results showed that PTR-SeNPs could significantly ameliorate APAP-induced oxidative injury as evidenced by a range of biochemical analysis,histopathological examination and immunoblotting study.PTR-SeNPs could hosphorylate and activate PKCδ,depress Keap1,and increase nuclear accumulation of Nrf2,resulting in upregulation of GCLC,GCLM,HO-1 and NQO-1 expression.Besides,PTR-SeNPs suppressed the biotransformation of APAP to generate intracellular ROS through CYP 2E1 inhibition,restoring the mitochondrial morphology.Furthermore,the protective effect of PTR-SeNPs against APAP induced hepatotoxicity was weakened as Nrf2 was depleted in vivo,indicating the pivotal role of Nrf2 signaling pathway in PTR-SeNPs mediated hepatoprotective efficacy.Being a potential hepatic protectant,PTR-SeNPs could serve as a new source of selenium supplement for health-promoting and biomedical applications.
文摘Nowadays,doctors and nutritionists recommend individuals incorporate selenium-rich foods such as nuts,cereals,and mushrooms into their regular diet to maintain fitness and overall health.Selenium nanoparticles(SeNPs)exhibit strong chemopreventive capabilities.The anticipations for SeNPs with enhanced and tunable bioactive activities have led to a keen interest in phytofabrication.In this study,the aqueous extract of Clerodendron phlomidis plant leaves was utilized for the synthesis of SeNPs.In traditional Indian medicine,this plant extract is recognized as a significant anti-diabetic agent.The flavonoids tetrahydroxylflavone,7-hydroxyflavanone,and 6,4’-dimethyl-7-acetoxy-scutellarein present in this plant leaf extract demonstrate excellent anticancer activity.These secondary metabolites exhibit the ability to reduce sodium selenite into SeNPs.At a concentration of 13μg/mL,the synthesized SeNPs effectively inhibited the proliferation of the HepG2 cell line.The results suggest that the SeNPs possess promising anti-cancer potential against liver cancer and can be considered as a therapeutic agent for liver cancer treatment.Additionally,the cell cycle arrest induced by SeNPs was further confirmed by the fluorescence-activated cell sorting(FACS)method,indicating that SeNPs could efficiently differentiate cancer cells from normal cells.Notably,it showed a significant improvement in diethylnitrosamine(DEN)-induced Swiss Wistar rat groups.This scientific investigation highlights the high anti-cancer potential of SeNPs,positioning them as a promising therapeutic agent for liver cancer treatment.
基金funded by the open Project of Guangdong Key Laboratory of Marine Materia(LMM2020-7)the science and technology projects in Guangzhou(202201020632,202201020638,202206010100,202201020655 and 202102010202)+2 种基金the Guangdong Natural Science Foundation(2020A1515110648)the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03)The Guangzhou Medical University Students’Science and Technology Innovation Project(02-408-2203-2079,2021AEK119).
文摘AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress.A safe and effective nano-drug system of functionalized selenium-containing nanoparticles(SeNPs)was developed with lentinan(LNT)(SeNPs@LNT).Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT(1,2,4,8,16,32μM)for 24-72 h to evaluate the cytotoxicity of selenium.GC2-spg cells were randomly divided into the following groups:control,hydrogen peroxide(H_(2)O_(2)),SeNPs@LNT,and H_(2)O_(2)+SeNPs@LNT groups.H_(2)O_(2)+SeNPs@LNT group was pretreated with SeNPs@LNT 4μM for 12 h,followed by H_(2)O_(2)600μM for 8 h.The cell viability decreased in the H_(2)O_(2) group and increased significantly in the SeNPs@LNT group.Compared with the H_(2)O_(2) group,the SeNPs@LNT+H_(2)O_(2) group exhibited obvious red fluorescence,indicating a higher level of mitochondrial membrane potential.The content of intracellular reactive oxygen species(ROS)in the SeNPs@LNT group reduced significantly,and the intensity of green fluorescence in the SeNPs@LNT+H_(2)O_(2) group decreased significantly compared with the H_(2)O_(2) group,indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress.The activity of GPx and SOD increased significantly in the SeNPs@LNT group.The expression of p53 decreased significantly under the intervention of nano-selenium,and GPx1 expression increased.In the oxidative stress group,the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups.Thus,SeNPs@LNT can promote GC2-spg cell proliferation,improve GPx and SOD activities,remove intracellular ROS,and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels.SeNPs@LNT has good biological activity and antioxidant effect,which can be used to protect the male reproductive system from oxidative stress.
基金Science and Technology Projects in Guangzhou(202201020632,202201020638,202206010100,202201020655,and 202102010202)the Guangdong Natural Science Foundation(2020A1515110648)+2 种基金the Open Project of Guangdong Key Laboratory of Marine Materia(LMM2020-7)the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03)the Guangzhou Medical University Students’Science and Technology Innovation Project(2021AEK119 and 02-408-2203-2079).
文摘Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to investigate its antioxidant effect.This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide(H_(2)O_(2))-induced Leydig cell line,TM3.Methods:The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed.The concentration of 4μM could significantly promote the growth of TM3 cells.Oxidative stress damage was caused using an 800μM concentration of hydrogen peroxide.The cells were divided into four groups:normal control group,oxidative stress treatment group,H_(2)O_(2)+SeNPs@LNT group,and SeNPs@LNT group.The H_(2)O_(2)+SeNPs@LNT group was pretreated with 4μM of SeNPs@LNT for 12 h,followed by 800μM of H_(2)O_(2)for 8 h.Results:Nano-selenium could significantly promote the proliferation and viability of TM3 cells.SeNPs@LNT treatment increased the level of mitochondrial membrane potential in normal cells and slowed down the decline in mitochondrial membrane potential level caused by oxidative stress injury.In addition,the increase in reactive oxygen species caused by oxidative stress was inhibited by SeNPs@LNT treatment.The apoptosis of TM3 cells was detected,and SeNPs@LNT alleviated the necrosis and apoptosis of TM3 cells induced by H_(2)O_(2).Nano-selenium plays a protective role against oxidative H_(2)O_(2)-induced stress injury in TM3 cells through the changes in the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and P53 pathway,and the expression levels of other related proteins,protein kinase B(AKT)and C3.Conclusion:SeNPs@LNT exhibited good biological activity and antioxidant effect and can thus be used to protect the male reproductive system from oxidative stress.
基金Zanjan University of Medical Sciences supported the present study(Grant Number:A-12-1244-18).
文摘In the present study,we investigated the synergistic effects of targeted methotrexate-selenium nanostructure containing Myc decoy oligodeoxynucleotides along with X-irradiation exposure as a combination therapy on LNCaP prostate cancer cells.Myc decoy ODNs were designed based on the promoter of Bcl-2 gene and analyzed by molecular docking and molecular dynamics assays.ODNs were loaded on the synthesized Se@BSA@Chi-MTX nanostructure.The physicochemical characteristics of nanostructures were determined by FTIR,DLS,UV-vis,TEM,EDX,in vitro release,and hemolysis tests.Subsequently,the cytotoxicity properties of them with and without X-irradiation were investigated by uptake,MTT,cell cycle,apoptosis,and scratch assays on the LNCaP cell line.The results of DLS and TEM showed negative charge(−9 mV)and nanometer size(40 nm)for Se@BSA@Chi-DEC-MTX NPs,respectively.The results of FTIR,UV-vis,and EDX showed the proper interaction of different parts and the correct synthesis of nanoparticles.The results of hemolysis showed the hemocompatibility of this nanoparticle in concentrations less than 6 mg/mL.The ODNs release from the nanostructures showed a pH-dependent manner,and the release rate was 15%higher in acidic pH.The targeted Se@BSA@Chi-labeled ODN-MTX NPs were efficiently taken up by LNCaP cells by targeting the prostate-specific membrane antigen(PSMA).The significant synergistic effects of nanostructure(containing MTX drug)treatment along with X-irradiation showed cell growth inhibition,apoptosis induction(~57%),cell cycle arrest(G2/M phase),and migration inhibition(up to 90%)compared to the control.The results suggested that the Se@BSA@Chi-DEC-MTX NPs can potentially suppress the cell growth of LNCaP cells.This nanostructure system can be a promising approach for targeted drug delivery and chemoradiotherapy in prostate cancer treatment.
文摘The well-distributed, stable selenium nanoparticles (10 nm) with good adhesive ability and biocompatibility were successfully synthesized by using the template of chitosan cross-linked with glutaradehyde. The resulting selenium nanoparticles were used as a new carrier for horseradish peroxidase to construct H2O2 biosensors with good performances.
文摘<span style="font-family:Verdana;">A simple method for assessment of the toxicity and antidote effect of selenium nanoparticles with </span><i><i><span style="font-family:Verdana;">Paramecium</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;"> is presented. Light microscopy in combination with computerized video tracking is employed for </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">determination of </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">survival time of <i></i></span><i><i><span style="font-family:Verdana;">P.</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i></span><span style="font-family:Verdana;">. Up to 800 mg/L, selenium nanoparticles are not acutely toxic. </span><span style="font-family:Verdana;">With</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> respect to a potential antidote effect, the lethality of silver nanoparticles, silver nitrate, sodium hydrogen selenite, and sodium selenite to <i></i></span><i><i><span style="font-family:Verdana;">P.</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i></span><span style="font-family:Verdana;"> was decreased and survival time was extend</span><span style="font-family:Verdana;">ed upon pre-treatment with selenium nanoparticles. Taken together, these findings suggest that administration of selenium nanoparticles attenuates</span><span style="font-family:Verdana;"> exposure </span><span style="font-family:Verdana;">to</span><span style="font-family:Verdana;"> toxicants. Selenium nanoparticles could be a good functional additive for food management in animals.</span>
文摘The association of gut microflora and human health is being increasingly recognized,and the impact of gut microflora on the host is well characterized,including the body’s energy metabolism and immune system maintenance.Several human diseases,including metabolic,autoimmune,obesity,hypothyroidism,and intestinal disorders,are closely associated with gut dysbiosis.Inorganic nanoparticles(NPs)are extensively utilized in numerous fields due to their distinctive,attractive physicochemical properties.Estimation of the potential impacts of NPs,with a high number of microorganisms inside the human body(microbiota)and its genomes(microbiome),represents one of the most important aspects of nano-toxicology.This review article aims to provide information on the association of gut microflora alterations to diseases and describe the impacts of various inorganic NPs,including silver,zinc,selenium,titania,silicon,and copper,on gut microflora.Research on the effect of inorganic NPs on gut microflora of animal models and the poultry industry is reviewed.The response of pathogenic Enterobacter species to inorganic NPs has been expounded in detail.This review also highlights the need to focus on the ancillary effects of various inorganic NPs on gut microflora to expedite the suitable advancement of these particles for future use.Finally,the key opportunistic areas for the application of nanotechnology are underlined to manipulate the microbiome of gut dysbiosis,provide an overview,and address potential challenges and our perspective on this evolving field.
基金funded by TJ04000198:Influence of selenium and vitamin E in wagyu hybrids on reproductive properties and meat performance.
文摘The productivity and sustainability of livestock production systems are heavily influenced by animal nutrition.To maintain homeostatic balance in the body of the animal at different phases of life,the percentage of organically active minerals in livestock feed must be optimized.Selenium(Se)is a crucial trace mineral that is required for the maintenance of many functions of the body.Se nanoparticles(SeNPs)attracted considerable interest from researchers for a variety of applications a decade ago,owing to their extraordinary properties.SeNPs offer significant advantages over larger-sized materials,by having a comparatively wider surface area,increased surface energy,and high volume.Despite its benefits,SeNP also has toxic effects,therefore safety concerns must be taken for a successful application.The toxicological effects of SeNPs in animals are characterized by weight loss,and increased mortality rate.A safe-by-strategy to certify animal,human and environmental safety will contribute to an early diagnosis of all risks associated with SeNPs.This review is aimed at describing the beneficial uses and potential toxicity of SeNPs in various animals.It will also serve as a summary of different levels of SeNPs which should be added in the feed of animals for better performance.
基金supported by the National Natural Science Foundation of China(No.32072746)the Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University(No.CX2021029,No.CX2022062).
文摘Selenium nanoparticles(SeNPs)are proposed as a safer and more effective selenium delivery system than sodium selenite(Na_(2)SeO_(3)).Here,we investigated the effects of replacing dietary Na_(2)SeO_(3)with SeNPs synthesized by Lactobacillus casei ATCC 393 on the growth performance and gut health of earlyweaned piglets.Seventy-two piglets(Duroc×Landrace×Large Yorkshire)weaned at 21 d of age were divided into the control group(basal diet containing 0.3 mg Se/kg from Na_(2)SeO_(3))and SeNPs group(basal diet containing 0.3 mg Se/kg from SeNPs)during a 14-d feeding period.The results revealed that SeNPs supplementation increased the average daily gain(P=0.022)and average daily feed intake(P=0.033),reduced(P=0.056)the diarrhea incidence,and improved(P=0.013)the feed conversion ratio compared with Na_(2)SeO_(3).Additionally,SeNPs increased jejunal microvilli height(P=0.006)and alleviated the intestinal barrier dysfunction by upregulating(P<0.05)the expression levels of mucin 2 and tight junction proteins,increasing(P<0.05)Se availability,and maintaining mitochondrial structure and function,thereby improving antioxidant capacity and immunity.Furthermore,metabolomics showed that SeNPs can regulate lipid metabolism and participate in the synthesis,secretion and action of parathyroid hormone,proximal tubule bicarbonate reclamation and tricarboxylic acid cycle.Moreover,SeNPs increased(P<0.05)the abundance of Holdemanella and the levels of acetate and propionate.Correlation analysis suggested that Holdemanella was closely associated with the regulatory effects of SeNPs on early-weaned piglets through participating in lipid metabolism.Overall,replacing dietary Na_(2)SeO_(3)with biogenic SeNPs could be a potential nutritional intervention strategy to prevent earlyweaning syndrome in piglets.