The pathogenesis of myelodysplastic syndrome(MDS)may be related to the abnormal expression of microRNAs(miRNAs),which could influence the differentiation capacity of mesenchymal stem cells(MSCs)towards adipogenic and ...The pathogenesis of myelodysplastic syndrome(MDS)may be related to the abnormal expression of microRNAs(miRNAs),which could influence the differentiation capacity of mesenchymal stem cells(MSCs)towards adipogenic and osteogenic lineages.In this study,exosomes from bone marrow plasma were successfully extracted and identified.Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls(NOR)and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs.Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro,while inhibition of miR-103-3p showed the opposite results in NOR-MSCs.Thus,the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs,significantly impacting MDS-MSCs differentiation.The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation,thereby providing possible target for the treatment of MDS pathogenesis.展开更多
BACKGROUND The hypoxic environment during bone healing is important in regulating the differentiation of periosteal stem cells(PSCs)into osteoblasts or chondrocytes;however,the underlying mechanisms remain unclear.AIM...BACKGROUND The hypoxic environment during bone healing is important in regulating the differentiation of periosteal stem cells(PSCs)into osteoblasts or chondrocytes;however,the underlying mechanisms remain unclear.AIM To determine the effect of hypoxia on PSCs,and the expression of microRNA-584-5p(miR-584-5p)and RUNX family transcription factor 2(RUNX2)in PSCs was modulated to explore the impact of the miR-584-5p/RUNX2 axis on hypoxiainduced osteogenic differentiation of PSCs.METHODS In this study,we isolated primary mouse PSCs and stimulated them with hypoxia,and the characteristics and functional genes related to PSC osteogenic differentiation were assessed.Constructs expressing miR-584-5p and RUNX2 were established to determine PSC osteogenic differentiation.RESULTS Hypoxic stimulation induced PSC osteogenic differentiation and significantly increased calcified nodules,intracellular calcium ion levels,and alkaline phosphatase(ALP)activity in PSCs.Osteogenic differentiation-related factors such as RUNX2,bone morphogenetic protein 2,hypoxia-inducible factor 1-alpha,and ALP were upregulated;in contrast,miR-584-5p was downregulated in these cells.Furthermore,upregulation of miR-584-5p significantly inhibited RUNX2 expression and hypoxia-induced PSC osteogenic differentiation.RUNX2 was the target gene of miR-584-5p,antagonizing miR-584-5p inhibition in hypoxia-induced PSC osteogenic differentiation.CONCLUSION Our study showed that the interaction of miR-584-5p and RUNX2 could mediate PSC osteogenic differentiation induced by hypoxia.展开更多
BACKGROUND Advanced glycation end products(AGEs)are diabetic metabolic toxic products that cannot be ignored.Nε-(carboxymethyl)lysine(CML),a component of AGEs,could increase macrophage lipid uptake,promote foam cell ...BACKGROUND Advanced glycation end products(AGEs)are diabetic metabolic toxic products that cannot be ignored.Nε-(carboxymethyl)lysine(CML),a component of AGEs,could increase macrophage lipid uptake,promote foam cell formation,and thereby accelerate atherosclerosis.The receptor for AGEs(RAGE)and cluster of differentiation 36(CD36)were the receptors of CML.However,it is still unknown whether RAGE and CD36 play key roles in CML-promoted lipid uptake.AIM Our study aimed to explore the role of RAGE and CD36 in CML-induced macrophage lipid uptake.METHODS In this study,we examined the effect of CML on lipid uptake by Raw264.7 macrophages.After adding 10 mmol/L CML,the lipid accumulation in macrophages was confirmed by oil red O staining.Expression changes of CD36 and RAGE were detected with immunoblotting and quantitative real-time polymerase chain reaction.The interaction between CML with CD36 and RAGE was verified by immunoprecipitation.We synthesized a novel N-succinimidyl-4-18Ffluorobenzoate-CML radioactive probe.Radioactive receptor-ligand binding assays were performed to test the binding affinity between CML with CD36 and RAGE.The effects of blocking CD36 or RAGE on CML-promoting lipid uptake were also detected.RESULTS The study revealed that CML significantly promoted lipid uptake by macrophages.Immunoprecipitation and radioactive receptor-ligand binding assays indicated that CML could specifically bind to both CD36 and RAGE.CML had a higher affinity for CD36 than RAGE.ARG82,ASN71,and THR70 were the potential interacting amino acids that CD36 binds to CML Anti-CD36 and anti-RAGE could block the uptake of CML by macrophages.The lipid uptake promotion effect of CML was significantly attenuated after blocking CD36 or RAGE.CONCLUSION Our results suggest that the binding of CML with CD36 and RAGE promotes macrophage lipid uptake.展开更多
<p style="text-align:justify;margin-left:10pt;"> <span style="font-family:;" "=""><span>This study examined the validity of the differentiation-of-self hypothesis f...<p style="text-align:justify;margin-left:10pt;"> <span style="font-family:;" "=""><span>This study examined the validity of the differentiation-of-self hypothesis for Japanese university students, and its mechanism in relation to negative life event aversion. Although </span><span>the differentiation-of-self hypothesis is accepted as one of the basic hypothes</span><span>es in the clinical field of family therapy in Japan</span><span><span>, empirical studies on the subject are scarce. Further, there are few studies, internationally, on its mechanism. A questionnaire survey was conducted with university students (</span><i><span>n </span></i><span>= 250) to measure their differentiation of self, maladaptive states, and negative life event aversion. </span><span>Compared to the better-differentiated group, the less-differentiated group had higher scores both for maladaptive </span></span><span><span>states—somatic symptoms (</span><i><span>t</span></i><span>(223) = -</span></span></span><span><span style="font-family:;" "=""><span><span>2.70, </span><i><span>p</span></i><span> = </span></span></span></span><span><span>0</span></span><span><span style="font-family:;" "=""><span>.008), social dysfunction</span><span><span> (</span><i><span>t</span></i><span>(211.00) = -</span></span></span></span><span><span style="font-family:;" "=""><span><span>3.30, </span><i><span>p</span></i><span> = </span></span></span></span><span><span>0</span></span><span><span style="font-family:;" "=""><span>.001), and severe depression (</span><i><span>t</span></i><span>(208.78) = -</span></span></span><span><span style="font-family:;" "=""><span><span>4.08, </span><i><span>p</span></i><span> <</span></span></span></span><span><span> 0</span></span><span><span style="font-family:;" "=""><span>.001)—and negative life event aversion—interpersonal domain (</span><i><span>t</span></i><span>(223) = -</span></span></span><span><span style="font-family:;" "=""><span><span>3.96, </span><i><span>p</span></i><span> <</span></span></span></span><span><span> 0</span></span><span><span style="font-family:;" "=""><span>.001) and achievement domain (</span><i><span>t</span></i><span>(223) = -</span></span></span><span><span style="font-family:;" "=""><span><span>3.83, </span><i><span>p</span></i><span> <</span></span></span></span><span><span> 0</span></span><span><span>.001). </span></span><span>The results supported the validity of the differentiation-of-self hypothesis for Japanese university students, and that cognitive tendencies toward negative life events may be part of the mechanism for less-differentiated individuals’ subsequent vulnerability. This suggests the effectiveness of cognitive interventions.</span> </p>展开更多
The regulation of adult neural stem cells(NSCs) is critical for lifelong neurogenesis. MicroRNAs(miRNAs) are a type of small, endogenous RNAs that regulate gene expression post-transcriptionally and influence signalin...The regulation of adult neural stem cells(NSCs) is critical for lifelong neurogenesis. MicroRNAs(miRNAs) are a type of small, endogenous RNAs that regulate gene expression post-transcriptionally and influence signaling networks responsible for several cellular processes. In this study, mi R-103-3 p was transfected into neural stem cells derived from embryonic hippocampal neural stem cells. The results showed that mi R-103-3 p suppressed neural stem cell proliferation and differentiation, and promoted apoptosis. In addition, mi R-103-3 p negatively regulated Nud E neurodevelopment protein 1-like 1(Ndel1) expression by binding to the 3′ untranslated region of Ndel1. Transduction of neural stem cells with a lentiviral vector overexpressing Ndel1 significantly increased cell proliferation and differentiation, decreased neural stem cell apoptosis, and decreased protein expression levels of Wnt3 a, β-catenin, phosphor-GSK-3β, LEF1, c-myc, c-Jun, and cyclin D1, all members of the Wnt/β-catenin signaling pathway. These findings suggest that Ndel1 is a novel mi R-103-3 p target and that mi R-103-3 p acts by suppressing neural stem cell proliferation and promoting apoptosis and differentiation. This study was approved by the Animal Ethics Committee of Nantong University, China(approval No. 20200826-003) on August 26, 2020.展开更多
Control parameters of original differential evolution (DE) are kept fixed throughout the entire evolutionary process. However, it is not an easy task to properly set control parameters in DE for different optiinizat...Control parameters of original differential evolution (DE) are kept fixed throughout the entire evolutionary process. However, it is not an easy task to properly set control parameters in DE for different optiinization problems. According to the relative position of two different individual vectors selected to generate a difference vector in the searching place, a self-adapting strategy for the scale factor F of the difference vector is proposed. In terms of the convergence status of the target vector in the current population, a self-adapting crossover probability constant CR strategy is proposed. Therefore, good target vectors have a lower CFI while worse target vectors have a large CFI. At the same time, the mutation operator is modified to improve the convergence speed. The performance of these proposed approaches are studied with the use of some benchmark problems and applied to the trajectory planning of a three-joint redundant manipulator. Finally, the experiment results show that the proposed approaches can greatly improve robustness and convergence speed.展开更多
For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the ef...For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the effect of gastric carcinoma cell differentiation by chemical inducers.Under light microscope, MGc80-3 cells, after treated with 1 mM dBcAMP, tended to be flat and disperse, and their volume gradually enlarged, with their uncleus relatively smaller and their shape rather regular. Morphological changes, like norma differentiated epithelial cells, were observed. The cells attached firmly, grew slowly, their growth curve showed inhibitory rate amounted to 52.87%, and cellular division exponent displayed their peak value 1.5 times less than that of MGc80-3 cells. It was clear that dBcAMP could effectively inhibit the multiplication activity of MGc80-3 cells. After dBcAMP treatment, remarkable changes of cell surface charges was indicated by cell electrophoresis, the ratio dropped to 3.043 from 3.988, and their re-tardant ratio reached up to 31.2%. cAMP content in cells after this treatment, detected by cAMP and cGMP radioimmunoassay, was enhanced by 2.42 times, and cAMP/cGMP ratio, by 1.73 times. Thus, cAMP level within MGc80-3 cells was raised obviously by dBcAMP. Heterotransplantation experiments showed that tuntorigenic rate of MGc80-5 cells (transplanted subcutaneously to BALB/c mice) amounted to 100%, and that of the cells after this treatment was only 5.6%. Their tumorigenic ability was extremely reduced.These results confirmed that dBcAMP was able to change malignant phenotypic characteristics of MGc80-3 cells and produce a reversed alteration: Thus, it has a remarkable inductive effect in differentiating gastric carcinoma cells. All these characteristics were also considered as the reference indexes in appraising reversed effect for the homologous cancer cells.展开更多
Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in me...Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects.展开更多
MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the potential function of the miR-181-5p family in chicken myogenesis remains largely unknown.Here,our study identified the two...MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the potential function of the miR-181-5p family in chicken myogenesis remains largely unknown.Here,our study identified the two chicken(Gallus gallus;Gga)miR-181-5p family members widely expressed in various tissues,specifically miR-181a-5p and miR-181b-5p.Besides,the breast muscles of fast-growing broilers expressed higher levels of miR-181a-5p and miR-181b-5p than those of slow-growing layers.Functionally,miR-181a-5p and miR-181b-5p both promote the expression level of myogenic factors including myogenin(MyoG),myogenic differentiation 1(MyoD1),and myosin heavy chain(MyHC),meanwhile accelerating the myotube formation of skeletal muscle satellite cells(SMSCs).Mechanistically,miR-181a-5p and miR-181b-5p directly bind to the 3′untranslated region(UTR)of the transforming growth factor beta receptor 1(TGFBR1)mRNA,further reducing the expression of TGFBR1.TGFBR1 is a key Transforming growth factor beta(TGF-β)signaling transduction receptor and had a negative function in muscle cell differentiation.Furthermore,knockdown of TGFBR1 facilitated the expression of chicken myogenic factors,boosted myotube formation,and decreased the SMAD family member 2/3(SMAD2/3)phosphorylation in chicken SMSCs.SMAD2/3 are downstream of TGF-βsignaling,and miR-181a-5p and miR-181b-5p could reduce the expression of TGFBR1 to further diminish the SMAD2/3 phosphorylation.Our findings revealed that the miR-181-5p family targets TGFBR1 to break the TGF-βsignaling transduction,which resulted in promoting chicken skeletal muscle development.展开更多
The current study examined the relationships between satisfaction with life and differentiation of self among Jewish and Arab women living inIsrael. The sample included 268 participants: 114 Jewish women and 154 Arab ...The current study examined the relationships between satisfaction with life and differentiation of self among Jewish and Arab women living inIsrael. The sample included 268 participants: 114 Jewish women and 154 Arab women. For both ethnic groups, satisfaction with life was negatively correlated with emotional cutoff. Among Arab women, satisfaction with life was found positively associated with I-position, marriage duration and education. The examination of cross-cultural differences yielded several findings: Arab women reported higher levels of emotional reactivity and I-position than Jewish women. No cultural differences were found in the levels of fusion with others, emotional cutoff and satisfaction with life. The current findings show that working women, who are highly differentiated, are satisfied with life to a greater extent than non-working women who are poorly differentiated. In general, the research findings provide support to the universality of the Family Systems Theory, and to the argument that differentiation of self is an important factor which may influence emotional wellbeing in all cultures.展开更多
The rise in the use of global polyester fiber contributed to strong demand of the Terephthalic acid(TPA).The liquid-phase catalytic oxidation of p-xylene(PX)to TPA is regarded as a critical and efficient chemical proc...The rise in the use of global polyester fiber contributed to strong demand of the Terephthalic acid(TPA).The liquid-phase catalytic oxidation of p-xylene(PX)to TPA is regarded as a critical and efficient chemical process in industry[1].PX oxidation reaction involves many complex side reactions,among which acetic acid combustion and PX combustion are the most important.As the target product of this oxidation process,the quality and yield of TPA are of great concern.However,the improvement of the qualified product yield can bring about the high energy consumption,which means that the economic objectives of this process cannot be achieved simultaneously because the two objectives are in conflict with each other.In this paper,an improved self-adaptive multi-objective differential evolution algorithm was proposed to handle the multi-objective optimization problems.The immune concept is introduced to the self-adaptive multi-objective differential evolution algorithm(SADE)to strengthen the local search ability and optimization accuracy.The proposed algorithm is successfully tested on several benchmark test problems,and the performance measures such as convergence and divergence metrics are calculated.Subsequently,the multi-objective optimization of an industrial PX oxidation process is carried out using the proposed immune self-adaptive multi-objective differential evolution algorithm(ISADE).Optimization results indicate that application of ISADE can greatly improve the yield of TPA with low combustion loss without degenerating TA quality.展开更多
A new admission control algorithm considering the network self-similar access characteristics is proposed. Taking advantage of the mathematical model of the network traffic admission control which can effectively over...A new admission control algorithm considering the network self-similar access characteristics is proposed. Taking advantage of the mathematical model of the network traffic admission control which can effectively overcome the self-similar characteristics of the network requests, through the scheduling of the differential service qucue based on priority while at the same time taking into account various factors including access characteristics of requests, load information, etc, smoothness of the admission control is ensured by the algorithm proposed in this paper. We design a non-linear self-adapting control algorithm by introducing an exponential admission function, thus overcomes the negative aspects introduced by static threshold parameters. Simulation results show that the scheme proposed in this paper can effectively improve the resource utilization of the clusters, while at the same time protecting the service with high priority. Our simulation results also show that this algorithm can improve system stability and reliability too. Key words Web cluster - admission control - differential service - self-similar - self-adapting CLC number TP 393 Foundation item: Supported by the National Natural Science Foundation of China (10375024) and the Hunan Natural Science Foundation of China(03JJY4054)Biography: LIU An-feng(1971-), male, Ph. D candidate, majoring in network computing, Web QoS.展开更多
Differential evolution(DE) demonstrates good convergence performance,but it is difficult to choose trial vector generation strategies and associated control parameter values.An improved method,self-adapting scalable D...Differential evolution(DE) demonstrates good convergence performance,but it is difficult to choose trial vector generation strategies and associated control parameter values.An improved method,self-adapting scalable DE(SSDE) algorithm,is proposed.Trial vector generation strategies and crossover probability are respectively self-adapted by two operators in this algorithm.Meanwhile,to enhance the convergence rate,vectors selected randomly with the optimal fitness values are introduced to guide searching direction.Benchmark problems are used to verify this algorithm.Compared with other well-known DE algorithms,experiment results indicate that this algorithm is better than other DE algorithms in terms of convergence rate and quality of optimization.展开更多
Yellowhorn (Xanthoceras sorbifolium Bunge) is a drought-tolerant tree in the family Sapindaceae that is native to northeastern China. Its remarkably oil-rich seeds are a reliable biodiesel source. An inter simple se...Yellowhorn (Xanthoceras sorbifolium Bunge) is a drought-tolerant tree in the family Sapindaceae that is native to northeastern China. Its remarkably oil-rich seeds are a reliable biodiesel source. An inter simple sequence repeat (ISSR) analysis showed genetic variation among four artificial populations in China: two in Inner Mongolia (IM), one in Liaoning (LN), and one in Shandong (SD). The average percentage of polymorphic loci was 81.25 % for these four populations. The Forest Farm in SD showed the highest number of effective alleles (Ne), Shannon index (I), and expected heterozygosity (He), i.e., 1.598, 0.470, and 0.325, respectively, but the lowest number of alleles (Na) as 1.600. Based on an analysis of molecular variance, 23 % of the total genetic variation was found among populations, and 77 % within populations. A principal coordinate analysis revealed two groups (Group 1: Lindonglinchang, Jianping Agriculture Research Station and Forest Farm; Group 2: Jinjilinchang). Understanding the genetic diversity among artificial yellowhorn populations in China, detected using ISSRs, will be useful for yellowhorn conservation and improvement. Additional arti-ficial and natural populations need to be included in the future for a country-wide perspective.展开更多
Fractional differential constitutive relationships are introduced to depict the history of dynamic stress inten- sity factors (DSIFs) for a semi-infinite crack in infinite viscoelastic material subjected to anti-pla...Fractional differential constitutive relationships are introduced to depict the history of dynamic stress inten- sity factors (DSIFs) for a semi-infinite crack in infinite viscoelastic material subjected to anti-plane shear impact load. The basic equations which govern the anti-plane deformation behavior are converted to a fractional wave-like equation. By utilizing Laplace and Fourier integral transforms, the fractional wave-like equation is cast into an ordinary differential equation (ODE). The unknown function in the solution of ODE is obtained by applying Fourier transform directly to the boundary conditions of fractional wave-like equation in Laplace domain instead of solving dual integral equations. Analytical solutions of DSIFs in Laplace domain are derived by Wiener-Hopf technique and the numerical solutions of DSIFs in time domain are obtained by Talbot algorithm. The effects of four parameters α, β, b1, b2 of the fractional dif- ferential constitutive model on DSIFs are discussed. The numerical results show that the present fractional differential constitutive model can well describe the behavior of DSIFs of anti-plane fracture in viscoelastic materials, and the model is also compatible with solutions of DSIFs of anti-plane fracture in elastic materials.展开更多
In the dry-sliding process of the woven self-lubricating liner which is used in the self-lubricating spherical plain bearing, the friction heat plays an important role in the tribological performances of the liner. It...In the dry-sliding process of the woven self-lubricating liner which is used in the self-lubricating spherical plain bearing, the friction heat plays an important role in the tribological performances of the liner. It has important value to study on the relationship between tribological performances of the liner and the friction heat. Unforttmately, up to now, published work on this relationship is quite scarce. Therefore, the effect of friction heat on the tribological performances of the liner was investigated in the present work. The tribological behaviors of the liner were evaluated by using the high temperature end surface wear tester. Scanning electron microscopy (SEM) was utilized to examine the morphologies of worn surfaces of the liner and study the failure modes. Differential scanning calorimetry (DSC) measurement and X-ray diffraction (XRD) analysis were performed to study the behaviors of the wear debris. The temperature rise on the worn surface was calculated according to classical models. SEM observation shows that the dominating wear mechanism for the liner is mainly affected by friction shear force, contact pressure and friction heat. Higher fusion heat for the wear debris than that for the pure polytetrafluroethylene (PTFE) indicates that the PTFE is the main portion of the wear debris, and, the PTFE in the wear debris shows a higher crystallisation degree owing to the effects of friction shear force and the friction heat. Combining the calculated temperature rise results with the wear rate of the liner, it can be concluded that the effects of temperature rise o n the tribological performances of the liner become more obvious when the temperature rise exceeds the glass transition temperature (Tg) of the PTFE. The wear resistance of the liner deteriorates dramatically when the temperature rise approaches to the melting point (Ton) of the PTFE. The tribological performances of the liner can be improved when the temperature rise exceeds Tg but is far lower than Ton- The present study on the relationship between the temperature rise and the tribological performances of the liner may provide the basis for further understanding of the wear mechanisms of the liner as well as the relationship between the formation of the PTFE transfer film and the friction heat during the dry-sliding of the Finer.展开更多
The element energy projection (EEP) method for computation of super- convergent resulting in a one-dimensional finite element method (FEM) is successfully used to self-adaptive FEM analysis of various linear probl...The element energy projection (EEP) method for computation of super- convergent resulting in a one-dimensional finite element method (FEM) is successfully used to self-adaptive FEM analysis of various linear problems, based on which this paper presents a substantial extension of the whole set of technology to nonlinear problems. The main idea behind the technology transfer from linear analysis to nonlinear analysis is to use Newton's method to linearize nonlinear problems into a series of linear problems so that the EEP formulation and the corresponding adaptive strategy can be directly used without the need for specific super-convergence formulation for nonlinear FEM. As a re- sult, a unified and general self-adaptive algorithm for nonlinear FEM analysis is formed. The proposed algorithm is found to be able to produce satisfactory finite element results with accuracy satisfying the user-preset error tolerances by maximum norm anywhere on the mesh. Taking the nonlinear ordinary differential equation (ODE) of second-order as the model problem, this paper describes the related fundamental idea, the imple- mentation strategy, and the computational algorithm. Representative numerical exam- ples are given to show the efficiency, stability, versatility, and reliability of the proposed approach.展开更多
基金This work was supported by The Nature Science Foundation of China(Nos.82070176,82070128,81900132)the Medical Science and Technology Research Fund of Guangdong Province(No.A2020585).
文摘The pathogenesis of myelodysplastic syndrome(MDS)may be related to the abnormal expression of microRNAs(miRNAs),which could influence the differentiation capacity of mesenchymal stem cells(MSCs)towards adipogenic and osteogenic lineages.In this study,exosomes from bone marrow plasma were successfully extracted and identified.Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls(NOR)and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs.Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro,while inhibition of miR-103-3p showed the opposite results in NOR-MSCs.Thus,the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs,significantly impacting MDS-MSCs differentiation.The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation,thereby providing possible target for the treatment of MDS pathogenesis.
基金Supported by Sailing Program of Naval Medical University,Program of Shanghai Hongkou District Health Commission,No.2202-27Special Funds for Activating Scientific Research of Shanghai Fourth People’s Hospital,No.sykyqd05801.
文摘BACKGROUND The hypoxic environment during bone healing is important in regulating the differentiation of periosteal stem cells(PSCs)into osteoblasts or chondrocytes;however,the underlying mechanisms remain unclear.AIM To determine the effect of hypoxia on PSCs,and the expression of microRNA-584-5p(miR-584-5p)and RUNX family transcription factor 2(RUNX2)in PSCs was modulated to explore the impact of the miR-584-5p/RUNX2 axis on hypoxiainduced osteogenic differentiation of PSCs.METHODS In this study,we isolated primary mouse PSCs and stimulated them with hypoxia,and the characteristics and functional genes related to PSC osteogenic differentiation were assessed.Constructs expressing miR-584-5p and RUNX2 were established to determine PSC osteogenic differentiation.RESULTS Hypoxic stimulation induced PSC osteogenic differentiation and significantly increased calcified nodules,intracellular calcium ion levels,and alkaline phosphatase(ALP)activity in PSCs.Osteogenic differentiation-related factors such as RUNX2,bone morphogenetic protein 2,hypoxia-inducible factor 1-alpha,and ALP were upregulated;in contrast,miR-584-5p was downregulated in these cells.Furthermore,upregulation of miR-584-5p significantly inhibited RUNX2 expression and hypoxia-induced PSC osteogenic differentiation.RUNX2 was the target gene of miR-584-5p,antagonizing miR-584-5p inhibition in hypoxia-induced PSC osteogenic differentiation.CONCLUSION Our study showed that the interaction of miR-584-5p and RUNX2 could mediate PSC osteogenic differentiation induced by hypoxia.
基金Supported by The National Natural Science Foundation of China,No.82070455Natural Science Foundation of Jiangsu Province,No.BK20201225Medical Innovation Team Project of Jiangsu Province,No.CXTDA2017010。
文摘BACKGROUND Advanced glycation end products(AGEs)are diabetic metabolic toxic products that cannot be ignored.Nε-(carboxymethyl)lysine(CML),a component of AGEs,could increase macrophage lipid uptake,promote foam cell formation,and thereby accelerate atherosclerosis.The receptor for AGEs(RAGE)and cluster of differentiation 36(CD36)were the receptors of CML.However,it is still unknown whether RAGE and CD36 play key roles in CML-promoted lipid uptake.AIM Our study aimed to explore the role of RAGE and CD36 in CML-induced macrophage lipid uptake.METHODS In this study,we examined the effect of CML on lipid uptake by Raw264.7 macrophages.After adding 10 mmol/L CML,the lipid accumulation in macrophages was confirmed by oil red O staining.Expression changes of CD36 and RAGE were detected with immunoblotting and quantitative real-time polymerase chain reaction.The interaction between CML with CD36 and RAGE was verified by immunoprecipitation.We synthesized a novel N-succinimidyl-4-18Ffluorobenzoate-CML radioactive probe.Radioactive receptor-ligand binding assays were performed to test the binding affinity between CML with CD36 and RAGE.The effects of blocking CD36 or RAGE on CML-promoting lipid uptake were also detected.RESULTS The study revealed that CML significantly promoted lipid uptake by macrophages.Immunoprecipitation and radioactive receptor-ligand binding assays indicated that CML could specifically bind to both CD36 and RAGE.CML had a higher affinity for CD36 than RAGE.ARG82,ASN71,and THR70 were the potential interacting amino acids that CD36 binds to CML Anti-CD36 and anti-RAGE could block the uptake of CML by macrophages.The lipid uptake promotion effect of CML was significantly attenuated after blocking CD36 or RAGE.CONCLUSION Our results suggest that the binding of CML with CD36 and RAGE promotes macrophage lipid uptake.
文摘<p style="text-align:justify;margin-left:10pt;"> <span style="font-family:;" "=""><span>This study examined the validity of the differentiation-of-self hypothesis for Japanese university students, and its mechanism in relation to negative life event aversion. Although </span><span>the differentiation-of-self hypothesis is accepted as one of the basic hypothes</span><span>es in the clinical field of family therapy in Japan</span><span><span>, empirical studies on the subject are scarce. Further, there are few studies, internationally, on its mechanism. A questionnaire survey was conducted with university students (</span><i><span>n </span></i><span>= 250) to measure their differentiation of self, maladaptive states, and negative life event aversion. </span><span>Compared to the better-differentiated group, the less-differentiated group had higher scores both for maladaptive </span></span><span><span>states—somatic symptoms (</span><i><span>t</span></i><span>(223) = -</span></span></span><span><span style="font-family:;" "=""><span><span>2.70, </span><i><span>p</span></i><span> = </span></span></span></span><span><span>0</span></span><span><span style="font-family:;" "=""><span>.008), social dysfunction</span><span><span> (</span><i><span>t</span></i><span>(211.00) = -</span></span></span></span><span><span style="font-family:;" "=""><span><span>3.30, </span><i><span>p</span></i><span> = </span></span></span></span><span><span>0</span></span><span><span style="font-family:;" "=""><span>.001), and severe depression (</span><i><span>t</span></i><span>(208.78) = -</span></span></span><span><span style="font-family:;" "=""><span><span>4.08, </span><i><span>p</span></i><span> <</span></span></span></span><span><span> 0</span></span><span><span style="font-family:;" "=""><span>.001)—and negative life event aversion—interpersonal domain (</span><i><span>t</span></i><span>(223) = -</span></span></span><span><span style="font-family:;" "=""><span><span>3.96, </span><i><span>p</span></i><span> <</span></span></span></span><span><span> 0</span></span><span><span style="font-family:;" "=""><span>.001) and achievement domain (</span><i><span>t</span></i><span>(223) = -</span></span></span><span><span style="font-family:;" "=""><span><span>3.83, </span><i><span>p</span></i><span> <</span></span></span></span><span><span> 0</span></span><span><span>.001). </span></span><span>The results supported the validity of the differentiation-of-self hypothesis for Japanese university students, and that cognitive tendencies toward negative life events may be part of the mechanism for less-differentiated individuals’ subsequent vulnerability. This suggests the effectiveness of cognitive interventions.</span> </p>
基金supported by Graduate Scientific Research Innovation Program of Jiangsu Province of China,No.KYCX192066(to WL)Project Funded by the Priority Academic Program Development(PAPD)of Jiangsu Higher Education institutions China,No.03081023(to GHJ)。
文摘The regulation of adult neural stem cells(NSCs) is critical for lifelong neurogenesis. MicroRNAs(miRNAs) are a type of small, endogenous RNAs that regulate gene expression post-transcriptionally and influence signaling networks responsible for several cellular processes. In this study, mi R-103-3 p was transfected into neural stem cells derived from embryonic hippocampal neural stem cells. The results showed that mi R-103-3 p suppressed neural stem cell proliferation and differentiation, and promoted apoptosis. In addition, mi R-103-3 p negatively regulated Nud E neurodevelopment protein 1-like 1(Ndel1) expression by binding to the 3′ untranslated region of Ndel1. Transduction of neural stem cells with a lentiviral vector overexpressing Ndel1 significantly increased cell proliferation and differentiation, decreased neural stem cell apoptosis, and decreased protein expression levels of Wnt3 a, β-catenin, phosphor-GSK-3β, LEF1, c-myc, c-Jun, and cyclin D1, all members of the Wnt/β-catenin signaling pathway. These findings suggest that Ndel1 is a novel mi R-103-3 p target and that mi R-103-3 p acts by suppressing neural stem cell proliferation and promoting apoptosis and differentiation. This study was approved by the Animal Ethics Committee of Nantong University, China(approval No. 20200826-003) on August 26, 2020.
基金This work was supported by the National Natural Science Foundation of China(No.60375001)the High School Doctoral Foundation of China(NO.20030532004).
文摘Control parameters of original differential evolution (DE) are kept fixed throughout the entire evolutionary process. However, it is not an easy task to properly set control parameters in DE for different optiinization problems. According to the relative position of two different individual vectors selected to generate a difference vector in the searching place, a self-adapting strategy for the scale factor F of the difference vector is proposed. In terms of the convergence status of the target vector in the current population, a self-adapting crossover probability constant CR strategy is proposed. Therefore, good target vectors have a lower CFI while worse target vectors have a large CFI. At the same time, the mutation operator is modified to improve the convergence speed. The performance of these proposed approaches are studied with the use of some benchmark problems and applied to the trajectory planning of a three-joint redundant manipulator. Finally, the experiment results show that the proposed approaches can greatly improve robustness and convergence speed.
文摘For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the effect of gastric carcinoma cell differentiation by chemical inducers.Under light microscope, MGc80-3 cells, after treated with 1 mM dBcAMP, tended to be flat and disperse, and their volume gradually enlarged, with their uncleus relatively smaller and their shape rather regular. Morphological changes, like norma differentiated epithelial cells, were observed. The cells attached firmly, grew slowly, their growth curve showed inhibitory rate amounted to 52.87%, and cellular division exponent displayed their peak value 1.5 times less than that of MGc80-3 cells. It was clear that dBcAMP could effectively inhibit the multiplication activity of MGc80-3 cells. After dBcAMP treatment, remarkable changes of cell surface charges was indicated by cell electrophoresis, the ratio dropped to 3.043 from 3.988, and their re-tardant ratio reached up to 31.2%. cAMP content in cells after this treatment, detected by cAMP and cGMP radioimmunoassay, was enhanced by 2.42 times, and cAMP/cGMP ratio, by 1.73 times. Thus, cAMP level within MGc80-3 cells was raised obviously by dBcAMP. Heterotransplantation experiments showed that tuntorigenic rate of MGc80-5 cells (transplanted subcutaneously to BALB/c mice) amounted to 100%, and that of the cells after this treatment was only 5.6%. Their tumorigenic ability was extremely reduced.These results confirmed that dBcAMP was able to change malignant phenotypic characteristics of MGc80-3 cells and produce a reversed alteration: Thus, it has a remarkable inductive effect in differentiating gastric carcinoma cells. All these characteristics were also considered as the reference indexes in appraising reversed effect for the homologous cancer cells.
基金supported by grants from the National Natural Science Foundation of China(82071150,82170934,81870743,8190104 and 82171001)。
文摘Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects.
基金supported by the National Key Research and Development Program of China(2022YFF10002020)Sichuan Science and Technology Program,China(2021YFYZ0007 and 2021YFYZ0031).
文摘MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the potential function of the miR-181-5p family in chicken myogenesis remains largely unknown.Here,our study identified the two chicken(Gallus gallus;Gga)miR-181-5p family members widely expressed in various tissues,specifically miR-181a-5p and miR-181b-5p.Besides,the breast muscles of fast-growing broilers expressed higher levels of miR-181a-5p and miR-181b-5p than those of slow-growing layers.Functionally,miR-181a-5p and miR-181b-5p both promote the expression level of myogenic factors including myogenin(MyoG),myogenic differentiation 1(MyoD1),and myosin heavy chain(MyHC),meanwhile accelerating the myotube formation of skeletal muscle satellite cells(SMSCs).Mechanistically,miR-181a-5p and miR-181b-5p directly bind to the 3′untranslated region(UTR)of the transforming growth factor beta receptor 1(TGFBR1)mRNA,further reducing the expression of TGFBR1.TGFBR1 is a key Transforming growth factor beta(TGF-β)signaling transduction receptor and had a negative function in muscle cell differentiation.Furthermore,knockdown of TGFBR1 facilitated the expression of chicken myogenic factors,boosted myotube formation,and decreased the SMAD family member 2/3(SMAD2/3)phosphorylation in chicken SMSCs.SMAD2/3 are downstream of TGF-βsignaling,and miR-181a-5p and miR-181b-5p could reduce the expression of TGFBR1 to further diminish the SMAD2/3 phosphorylation.Our findings revealed that the miR-181-5p family targets TGFBR1 to break the TGF-βsignaling transduction,which resulted in promoting chicken skeletal muscle development.
文摘The current study examined the relationships between satisfaction with life and differentiation of self among Jewish and Arab women living inIsrael. The sample included 268 participants: 114 Jewish women and 154 Arab women. For both ethnic groups, satisfaction with life was negatively correlated with emotional cutoff. Among Arab women, satisfaction with life was found positively associated with I-position, marriage duration and education. The examination of cross-cultural differences yielded several findings: Arab women reported higher levels of emotional reactivity and I-position than Jewish women. No cultural differences were found in the levels of fusion with others, emotional cutoff and satisfaction with life. The current findings show that working women, who are highly differentiated, are satisfied with life to a greater extent than non-working women who are poorly differentiated. In general, the research findings provide support to the universality of the Family Systems Theory, and to the argument that differentiation of self is an important factor which may influence emotional wellbeing in all cultures.
基金Supported by the Shanghai Second Polytechnic University Key Discipline Construction-Control Theory & Control Engineering(No.XXKPY1609)the National Natural Science Foundation of China(61422303)+1 种基金Shanghai Talent Development Funding(H200-2R-15111)2017 Shanghai Second Polytechnic University Cultivation Research Program of Young Teachers(02)
文摘The rise in the use of global polyester fiber contributed to strong demand of the Terephthalic acid(TPA).The liquid-phase catalytic oxidation of p-xylene(PX)to TPA is regarded as a critical and efficient chemical process in industry[1].PX oxidation reaction involves many complex side reactions,among which acetic acid combustion and PX combustion are the most important.As the target product of this oxidation process,the quality and yield of TPA are of great concern.However,the improvement of the qualified product yield can bring about the high energy consumption,which means that the economic objectives of this process cannot be achieved simultaneously because the two objectives are in conflict with each other.In this paper,an improved self-adaptive multi-objective differential evolution algorithm was proposed to handle the multi-objective optimization problems.The immune concept is introduced to the self-adaptive multi-objective differential evolution algorithm(SADE)to strengthen the local search ability and optimization accuracy.The proposed algorithm is successfully tested on several benchmark test problems,and the performance measures such as convergence and divergence metrics are calculated.Subsequently,the multi-objective optimization of an industrial PX oxidation process is carried out using the proposed immune self-adaptive multi-objective differential evolution algorithm(ISADE).Optimization results indicate that application of ISADE can greatly improve the yield of TPA with low combustion loss without degenerating TA quality.
文摘A new admission control algorithm considering the network self-similar access characteristics is proposed. Taking advantage of the mathematical model of the network traffic admission control which can effectively overcome the self-similar characteristics of the network requests, through the scheduling of the differential service qucue based on priority while at the same time taking into account various factors including access characteristics of requests, load information, etc, smoothness of the admission control is ensured by the algorithm proposed in this paper. We design a non-linear self-adapting control algorithm by introducing an exponential admission function, thus overcomes the negative aspects introduced by static threshold parameters. Simulation results show that the scheme proposed in this paper can effectively improve the resource utilization of the clusters, while at the same time protecting the service with high priority. Our simulation results also show that this algorithm can improve system stability and reliability too. Key words Web cluster - admission control - differential service - self-similar - self-adapting CLC number TP 393 Foundation item: Supported by the National Natural Science Foundation of China (10375024) and the Hunan Natural Science Foundation of China(03JJY4054)Biography: LIU An-feng(1971-), male, Ph. D candidate, majoring in network computing, Web QoS.
基金National Natural Science Foundation of China (No. 70971020)
文摘Differential evolution(DE) demonstrates good convergence performance,but it is difficult to choose trial vector generation strategies and associated control parameter values.An improved method,self-adapting scalable DE(SSDE) algorithm,is proposed.Trial vector generation strategies and crossover probability are respectively self-adapted by two operators in this algorithm.Meanwhile,to enhance the convergence rate,vectors selected randomly with the optimal fitness values are introduced to guide searching direction.Benchmark problems are used to verify this algorithm.Compared with other well-known DE algorithms,experiment results indicate that this algorithm is better than other DE algorithms in terms of convergence rate and quality of optimization.
基金supported by the Cooperative Research Program for Agriculture Science & Technology Development(Project no.PJ01117902) of the National Institute of Horticultural and Herbal Science,RDA,Republic of Koreathe Ministry of Education,People’s Republic of China(Program No.MS2012DBLY017 for Foreign Distinguished Scientists of the Ministry of Education)the Research Foundation of Kangwon National University,Republic of Korea
文摘Yellowhorn (Xanthoceras sorbifolium Bunge) is a drought-tolerant tree in the family Sapindaceae that is native to northeastern China. Its remarkably oil-rich seeds are a reliable biodiesel source. An inter simple sequence repeat (ISSR) analysis showed genetic variation among four artificial populations in China: two in Inner Mongolia (IM), one in Liaoning (LN), and one in Shandong (SD). The average percentage of polymorphic loci was 81.25 % for these four populations. The Forest Farm in SD showed the highest number of effective alleles (Ne), Shannon index (I), and expected heterozygosity (He), i.e., 1.598, 0.470, and 0.325, respectively, but the lowest number of alleles (Na) as 1.600. Based on an analysis of molecular variance, 23 % of the total genetic variation was found among populations, and 77 % within populations. A principal coordinate analysis revealed two groups (Group 1: Lindonglinchang, Jianping Agriculture Research Station and Forest Farm; Group 2: Jinjilinchang). Understanding the genetic diversity among artificial yellowhorn populations in China, detected using ISSRs, will be useful for yellowhorn conservation and improvement. Additional arti-ficial and natural populations need to be included in the future for a country-wide perspective.
基金supported by the National Natural Science Foundation of China(11072060)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Fractional differential constitutive relationships are introduced to depict the history of dynamic stress inten- sity factors (DSIFs) for a semi-infinite crack in infinite viscoelastic material subjected to anti-plane shear impact load. The basic equations which govern the anti-plane deformation behavior are converted to a fractional wave-like equation. By utilizing Laplace and Fourier integral transforms, the fractional wave-like equation is cast into an ordinary differential equation (ODE). The unknown function in the solution of ODE is obtained by applying Fourier transform directly to the boundary conditions of fractional wave-like equation in Laplace domain instead of solving dual integral equations. Analytical solutions of DSIFs in Laplace domain are derived by Wiener-Hopf technique and the numerical solutions of DSIFs in time domain are obtained by Talbot algorithm. The effects of four parameters α, β, b1, b2 of the fractional dif- ferential constitutive model on DSIFs are discussed. The numerical results show that the present fractional differential constitutive model can well describe the behavior of DSIFs of anti-plane fracture in viscoelastic materials, and the model is also compatible with solutions of DSIFs of anti-plane fracture in elastic materials.
文摘In the dry-sliding process of the woven self-lubricating liner which is used in the self-lubricating spherical plain bearing, the friction heat plays an important role in the tribological performances of the liner. It has important value to study on the relationship between tribological performances of the liner and the friction heat. Unforttmately, up to now, published work on this relationship is quite scarce. Therefore, the effect of friction heat on the tribological performances of the liner was investigated in the present work. The tribological behaviors of the liner were evaluated by using the high temperature end surface wear tester. Scanning electron microscopy (SEM) was utilized to examine the morphologies of worn surfaces of the liner and study the failure modes. Differential scanning calorimetry (DSC) measurement and X-ray diffraction (XRD) analysis were performed to study the behaviors of the wear debris. The temperature rise on the worn surface was calculated according to classical models. SEM observation shows that the dominating wear mechanism for the liner is mainly affected by friction shear force, contact pressure and friction heat. Higher fusion heat for the wear debris than that for the pure polytetrafluroethylene (PTFE) indicates that the PTFE is the main portion of the wear debris, and, the PTFE in the wear debris shows a higher crystallisation degree owing to the effects of friction shear force and the friction heat. Combining the calculated temperature rise results with the wear rate of the liner, it can be concluded that the effects of temperature rise o n the tribological performances of the liner become more obvious when the temperature rise exceeds the glass transition temperature (Tg) of the PTFE. The wear resistance of the liner deteriorates dramatically when the temperature rise approaches to the melting point (Ton) of the PTFE. The tribological performances of the liner can be improved when the temperature rise exceeds Tg but is far lower than Ton- The present study on the relationship between the temperature rise and the tribological performances of the liner may provide the basis for further understanding of the wear mechanisms of the liner as well as the relationship between the formation of the PTFE transfer film and the friction heat during the dry-sliding of the Finer.
基金supported by the National Natural Science Foundation of China(Nos.51378293,51078199,50678093,and 50278046)the Program for Changjiang Scholars and the Innovative Research Team in University of China(No.IRT00736)
文摘The element energy projection (EEP) method for computation of super- convergent resulting in a one-dimensional finite element method (FEM) is successfully used to self-adaptive FEM analysis of various linear problems, based on which this paper presents a substantial extension of the whole set of technology to nonlinear problems. The main idea behind the technology transfer from linear analysis to nonlinear analysis is to use Newton's method to linearize nonlinear problems into a series of linear problems so that the EEP formulation and the corresponding adaptive strategy can be directly used without the need for specific super-convergence formulation for nonlinear FEM. As a re- sult, a unified and general self-adaptive algorithm for nonlinear FEM analysis is formed. The proposed algorithm is found to be able to produce satisfactory finite element results with accuracy satisfying the user-preset error tolerances by maximum norm anywhere on the mesh. Taking the nonlinear ordinary differential equation (ODE) of second-order as the model problem, this paper describes the related fundamental idea, the imple- mentation strategy, and the computational algorithm. Representative numerical exam- ples are given to show the efficiency, stability, versatility, and reliability of the proposed approach.