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A SELF-ADJUST GROWTH OF CLUSTER——RESEARCH ON CLUSTER GROWTH MORPHOLOGY
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作者 王泽新 陈宗淇 《Chinese Science Bulletin》 SCIE EI CAS 1991年第9期725-728,共4页
Ⅰ. INTRODUCTIONAn extensive investigation has been conducted on cluster growth in experiment and theoretical simulation since 1980. In particular, the mutual advancement between theory and experiment results in the d... Ⅰ. INTRODUCTIONAn extensive investigation has been conducted on cluster growth in experiment and theoretical simulation since 1980. In particular, the mutual advancement between theory and experiment results in the deep understanding of cluster growth morphology and its kinetics. 展开更多
关键词 MONTE Carlo SIMULATION cluster growth simulation.
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IMPACTS ON THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN ISCHEMIC BRAIN TISSUE TREATED WITH CLUSTER PUNC-TURE ON SCALP ACUPOINTS IN RATS 被引量:5
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作者 李宝栋 王志勇 +5 位作者 白晶 白海娟 公维志 闫瑞萍 刘振江 王利春 《World Journal of Acupuncture-Moxibustion》 2008年第1期38-44,共7页
Objective To probe into the impacts on the expression of vascular endothelial growth factor (VEGF) in ischemic brain tissue treated with cluster puncture on scalp acupoints in rats, Methods 128 rats were randomized ... Objective To probe into the impacts on the expression of vascular endothelial growth factor (VEGF) in ischemic brain tissue treated with cluster puncture on scalp acupoints in rats, Methods 128 rats were randomized into pseudo-operation group, model group, scalp-needling group and cluster-needling group, In scalp-needling group, penetration method was used on the focal side from Bǎihuì (百会 GV20) to Qǔbīn (曲鬓 GB7), and in cluster-needling group, penetration method was used on both sides from GV20 to GB7, and a common needling on GV 20. Needles were punctured 2 mm in depth, constantly rotated for 10min, retained for 2 h. Immunohistrochemical method was applied to determine VEGF expression and microvessel density (MVD). Results With the intervention of cluster puncture on scalp acupoints, VEGF: expressions on every time-spot after ischemia were enhanced apparently, superior to those in scalp-needling group. On the three time-spots of the 7^th, 14^th and 21^st days, MVD was increased after cluster puncture on scale acupoints, superior to those in scalp-needling group. Conclusion Cluster puncture on scalp acupoints up-regulated VEGF expression and promoted regeneration of microvessel. 展开更多
关键词 cluster puncture on scalp acupoints Rat Ischemia Vascular endothelial growth factor (VEGF)
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Cluster planting impact on cotton growth, yield and biomass accumulation in an arid region oasis
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作者 Ting Ting Xie Pei Xi Su +1 位作者 Wen Zhi Zhao Li Shan Shan 《Research in Cold and Arid Regions》 CSCD 2018年第3期271-278,共8页
The cluster planting pattern (3 plants per hole) for cotton (Gossypium hirsutum L.) may increase economic yield over those of the traditional planting pattern (1 plant per hole) in arid regions of China. This in... The cluster planting pattern (3 plants per hole) for cotton (Gossypium hirsutum L.) may increase economic yield over those of the traditional planting pattern (1 plant per hole) in arid regions of China. This increase in yield depends on either increased biomass production or greater partitioning to fruit. This study was conducted to determine whether differences in biomass accumulation or partitioning to reproductive growth contributed to higher yield in the cluster planting pattern compared with the traditional one. Growth parameters, biomass accumulation, crop growth rate and partitioning between cluster planting pattern and traditional planting pattern was compared in northwest of China. The biomass production and partitioning in cluster planting plot was higher than in traditional planting one. Biomass accumulation was faster early in the clustered treatment, and it was also higher at harvest time. Total dry matter production per unit area was significantly higher than in the traditional planting. On a per plant basis, dry matter accumulation was faster and total biomass production was significantly higher in the cluster planting pattern. Numbers of sympodia and boll sizes were also larger, indicating that facilitation among plants was promoting crop yield. The increase in yield in the cluster planting treatment occurred through increased partitioning of dry matter to fruits than in the traditional planting pattern, resulting in more bolls and increased lint yield in arid regions. 展开更多
关键词 cluster planting growth parameter BIOMASS crop growth rate distribution ratio
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The Analysis of Growth and Reproduction of Industrial Clusters Based on View of Ecological Coexistence
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作者 LIU Man-feng Song Ying 《Journal of Mathematics and System Science》 2014年第3期186-193,共8页
The behavior of industrial cluster is similar to the behavior of biological population, that is, the industrial cluster is seemed to be an ecological symbiotic system. The relationship between economic organizations i... The behavior of industrial cluster is similar to the behavior of biological population, that is, the industrial cluster is seemed to be an ecological symbiotic system. The relationship between economic organizations in the industrial cluster is similar to the relationship of species in ecological system, and symbiotic reciprocity, competition and cooperation are the basic. The ecological development is mainstream and direction of industrial cluster. In this paper, firstly, we studied the ecological symbiosis growth model of industry cluster, and tbund it being according to logistic growth model; secondly, we studied the growth and reproduction of economic organizations in industrial cluster in three different cases, which with only competition relations, only beneficial relations, and both competition and beneficial cooperation relationship between economic organizations, and finally, we determined the number of economical organizations when the industrial cluster attained ecological equilibrium. 展开更多
关键词 Ecological coexistence industrial clusters growth and reproduction.
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基于分子束外延技术可控制备Bi原子团簇的研究
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作者 马玉麟 郭祥 丁召 《原子与分子物理学报》 CAS 北大核心 2025年第2期79-84,共6页
本研究基于分子束外延(MBE)技术在Si(111)衬底表面成功制备金属Bi原子团簇.首先,分别在100℃、125℃、150℃、175℃、200℃的生长温度下,制备了大小均一、密度不同的Bi原子团簇.实验结果表明,可以通过改变生长温度来精细控制Bi原子团簇... 本研究基于分子束外延(MBE)技术在Si(111)衬底表面成功制备金属Bi原子团簇.首先,分别在100℃、125℃、150℃、175℃、200℃的生长温度下,制备了大小均一、密度不同的Bi原子团簇.实验结果表明,可以通过改变生长温度来精细控制Bi原子团簇的密度,当温度升高100℃,密度从1.05×10^(11)cm^(-2)降低至2.5×10^(7)cm^(-2),实现对团簇密度4个数量级的可控调节,并且发现Bi原子团簇密度对生长温度的依赖性符合经典成核理论.其次,分别在10 s、15 s、20 s的沉积时长下,制备了密度相同、尺寸各异的Bi原子团簇.实验结果表明,可以通过改变沉积时长来精细控制Bi原子团簇的尺寸:当沉积时长增加10 s,高度和直径分别从8.5 nm和65 nm增大到13.7 nm和100 nm,实现对团簇尺寸在10 nm高度、80 nm直径范围的可控调节,并且发现Bi原子团簇尺寸对沉积时长的依赖性符合晶体生长动力学.与分子束外延制备传统的Ⅲ族(Al,Ga,In)原子团簇做对比,这些结果可以为制备Ⅴ族原子团簇提供实验参考和指导,从而促进纳米级含Bi材料的制备. 展开更多
关键词 分子束外延 Bi原子团簇 生长温度 沉积时长 经典成核理论 晶体生长动力学
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集群系统中的FP-Growth并行算法 被引量:8
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作者 陈敏 李徽翡 《计算机工程》 CAS CSCD 北大核心 2009年第20期71-72,75,共3页
针对FP-Growth算法面临大规模数据库时空效率不高的问题,提出一种面向计算机集群的并行算法。采用投影方法直接寻找频繁项的条件数据库,将挖掘条件数据库的工作分化成若干独立的子任务,分配到集群中的节点上并行实现,由中央节点汇总结... 针对FP-Growth算法面临大规模数据库时空效率不高的问题,提出一种面向计算机集群的并行算法。采用投影方法直接寻找频繁项的条件数据库,将挖掘条件数据库的工作分化成若干独立的子任务,分配到集群中的节点上并行实现,由中央节点汇总结果并输出。结果证明,该算法不仅能够提高计算速度,解决数据库规模过大时内存溢出的情况,且具有良好的延展性。 展开更多
关键词 FP-growth算法 计算机集群 并行算法
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胶体粒子絮凝、沉降的计算机模拟 Ⅰ 格子模型对Cluster生长形态的影响
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作者 王泽新 徐政龄 +3 位作者 赵长贵 张文霞 张积树 陈宗淇 《青岛化工学院学报(自然科学版)》 1991年第4期70-74,共5页
用Cluster生长自调整模型(SAGC),考察了不同格子模型条件下,Cluster的生长形态,研究了不同格子模型模拟得到的Cluster的分式维数D的变化,据此说明格子模型对Cluster生长形态的影响。结果得出,格子模型对Cluster的生形态有影响,呈现的明... 用Cluster生长自调整模型(SAGC),考察了不同格子模型条件下,Cluster的生长形态,研究了不同格子模型模拟得到的Cluster的分式维数D的变化,据此说明格子模型对Cluster生长形态的影响。结果得出,格子模型对Cluster的生形态有影响,呈现的明显规律是:D无格子<D四方格子<D三角格子,但其数值相差较小,可认为无影响。 展开更多
关键词 格子模型 cluster 生长形态 模拟
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面向计算机集群系统的FP-Growth算法的并行计算 被引量:1
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作者 陈敏 《中国管理信息化》 2009年第15期36-38,共3页
FP-Growth是频繁模式挖掘的经典算法,能够在不产生候选集的情况下生成所有的频繁模式,效率与Apri-ori算法相比有巨大提高,然而FP-Growth算法在挖掘频繁模式过程中需要递归构建大量的条件FP-tree,并分别针对这些条件FP-tree进行挖掘,时... FP-Growth是频繁模式挖掘的经典算法,能够在不产生候选集的情况下生成所有的频繁模式,效率与Apri-ori算法相比有巨大提高,然而FP-Growth算法在挖掘频繁模式过程中需要递归构建大量的条件FP-tree,并分别针对这些条件FP-tree进行挖掘,时间及空间效率不高,在实际应用中存在很大局限性。计算机集群是由多台普通计算机设备通过特定方式结合在一起构成的并行处理系统,属于分布式计算环境,具有计算能力强大、性价比高、灵活等优势。本文提出一种面向计算机集群的并行挖掘算法Gridify FP-Growth,该算法以FP-Growth为基础,通过任务划分的形式,将计算任务分配到计算机集群中各个计算节点上执行,充分利用各个节点的计算资源,最后汇总各节点的计算结果。实验证明Gridify FP-Growth算法不会牺牲计算的准确性,并可以大幅度缩短计算时间,有效缓解计算大规模数据库时的内存压力。 展开更多
关键词 频繁模式 FP—growth 并行计算 计算机集群
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Cluster生长的理论研究——Ⅰ分式维数和格子格型
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作者 王泽新 张积树 +3 位作者 张文霞 郝策 韩恩山 陈宗淇 《青岛化工学院学报(自然科学版)》 1991年第2期26-30,共5页
本文分析了Cluster生长的几种典型模型。并着重对这些模型中的重要概念——分式几何、分式维数和格子模型给予详细的论述。
关键词 cluster 生长动力学 胶体
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Cluster生长动力学的研究
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作者 王泽新 张积树 +3 位作者 张文霞 韩恩山 郝策 陈宗淇 《青岛化工学院学报(自然科学版)》 1991年第1期44-48,共5页
在Clutser生长自调整模型(SAG)的基础上,运用化学反应动力学的思想进行了Cluster生长动力学简单分析。用计算模拟的方法给出了生长控制、扩散控制的动力学曲线。在定性上模拟结果和实验结果符合得相当好。
关键词 cluster 生长动力学 胶体
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胶体粒子絮凝、沉降的计算机模拟 Ⅱ 粘附系数对Cluster生长形态的影响
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作者 王泽新 徐政龄 +2 位作者 张积树 陈宗淇 朱令之 《青岛化工学院学报(自然科学版)》 1992年第1期42-45,共4页
用Cluster生长的自调整模型,考查了不同粘附系数下的Cluster生长形态.结果表明,随着粘附系数的逐渐减小,Cluster生长形态逐渐由开放的树枝形结构过渡到致密的结晶型结构.并且在粘附系数很小时,Cluster的形态将显示出格子模型的影响.
关键词 胶体 粘附系数 计算机 自调整模型
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Analytical results for the cluster size distribution in controlled deposition processes
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作者 柯见洪 陈效双 林振权 《Chinese Physics B》 SCIE EI CAS CSCD 2010年第2期401-406,共6页
This paper proposes a controlled particle deposition model for cluster growth on the substrate surface and then presents exact results for the cluster (island) size distribution. In the system, at every time step a ... This paper proposes a controlled particle deposition model for cluster growth on the substrate surface and then presents exact results for the cluster (island) size distribution. In the system, at every time step a fixed number of particles are injected into the system and immediately deposited onto the substrate surface. It investigates the cluster size distribution by employing the generalized rate equation approach. The results exhibit that the evolution behaviour of the system depends crucially on the details of the adsorption rate kernel. The cluster size distribution can take the Poisson distribution or the conventional scaling form in some cases, while it is of a quite complex form in other cases. 展开更多
关键词 cluster growth DEPOSITION kinetic behaviour
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A MATHEMATICAL EXPRESSION OF CRYSTAL GROWTH RATE DURING CRYSTALLIZATION OF AMORPHOUS ALLOYS
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作者 LU Ke WANG Jingtang, National Laboratory of RSA, Institute of Metal Research, Academia Sinica, Shenyang, China LU Ke, Associate Professor, Institute of Metal Research, Academia Sinica, Shenyang 110015, China 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 1992年第7期33-37,共5页
A mathematical expression of the crystal growth rate during crystallization of the amorphous alloys was derived from the micromechanism of crystallization newly developed by the authors. Thus, the satisfactory explana... A mathematical expression of the crystal growth rate during crystallization of the amorphous alloys was derived from the micromechanism of crystallization newly developed by the authors. Thus, the satisfactory explanation of the experimental results obtained by Nunogaki et al., Heimendahl et al. and the authors might be found. It seems also to be modelled with the expression for the crystal growth and the crystal size influenced by time during the crystallization of amorphous Ni-P alloy foil at in situ heating. Based on the expression, the factors influencing the crystal growth rate, such as temperature, time and microstructure of amorphous alloys have been discussed. 展开更多
关键词 amorphous alloy crystal growth rate ordered cluster shearing deposition atomic diffusion
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Otolith Microstructure Analysis Reveals Different Growth Histories of Japanese Sardine (Sardinops melanostictus) in the Oyashio Waters
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作者 LIU Chunlin ZHANG Chi +4 位作者 TIAN Yongjun WANG Liangming LIN Longshan LI Yuan WATANABE Yoshiro 《Journal of Ocean University of China》 SCIE CAS CSCD 2022年第1期236-242,共7页
After decades of low year classes,the stock of Japanese sardine(Sardinops melanostictus)has begun to recover since the mid-2000s.The hatch dates and otolith growth rates of age-0 juvenile sardine,which were collected ... After decades of low year classes,the stock of Japanese sardine(Sardinops melanostictus)has begun to recover since the mid-2000s.The hatch dates and otolith growth rates of age-0 juvenile sardine,which were collected in the subarctic Oyashio waters in autumn 2018,were determined from an otolith microstructure analysis.The sardines were hatched from late January to late April,while mostly in February and March.The otolith growth rate increased continuously up to 60 d after hatching and thereafter de-creased.The revealed growth rate in a crucial growth period is faster than that reported for juvenile sardines collected in the 1990s,which is coincided with the recent recovery trend of the sardine stock.Two groups with different hatch dates,growth histories,and migration routes were identified using unsupervised random forest clustering analysis.They were considered inshore and offshore migration individuals in accordance with recent researches.In the offshore group,a high proportion of sardine juveniles hatched late and grew faster in the Kuroshio-Oyashio transitional waters,a finding consistent with the hypothesis of growth-rate-dependent re-cruitment.This finding on the population composition and growth rate of juvenile sardine in the Oyashio waters can be a basis for an improved prediction of their survival and provides us with valuable information on the recruitment processes of this stock during the period of stock recovery. 展开更多
关键词 Sardinops melanostictus otolith microstructure growth history unsupervised random forest clustering RECRUITMENT
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改进的FP-Growth算法在个性化推荐中的应用研究 被引量:1
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作者 刘玥波 徐田翔 徐国庆 《软件》 2021年第8期45-47,共3页
文章研究了聚类算法K-means和关联规则算法FP-Growth,针对两种算法存在的不足,提出了对原始事务数据库采用K-means凝聚层次算法按购物用户的属性特征进行聚类,并针对聚类压缩后事务数据库,利用矩阵存储数据并按支持度计数排序,并将其应... 文章研究了聚类算法K-means和关联规则算法FP-Growth,针对两种算法存在的不足,提出了对原始事务数据库采用K-means凝聚层次算法按购物用户的属性特征进行聚类,并针对聚类压缩后事务数据库,利用矩阵存储数据并按支持度计数排序,并将其应用于在线购物系统的个性化推荐中。算法的提出压缩了事务数据库,降低了生成频繁1项集的时间,减少了FP-Growth算法扫描数据库的次数,提高了算法的执行效率。 展开更多
关键词 K-MEANS算法 凝聚层次聚类 FP-growth算法
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Atomic mobility in energetic cluster deposition
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作者 PANZheng-Ying WANGYue-Xia WEIQi LIZhi-Jie ZHOULiang ZHANGLiang-Kun 《Nuclear Science and Techniques》 SCIE CAS CSCD 2004年第6期321-327,共7页
This paper tries to outline the influence of atomic mobility on the initial fabrication of thin films formed by LECBD. Based on our recent studies on low-energy cluster beam deposition (LECBD) by molecular dynamics si... This paper tries to outline the influence of atomic mobility on the initial fabrication of thin films formed by LECBD. Based on our recent studies on low-energy cluster beam deposition (LECBD) by molecular dynamics simulation, two examples, the deposition of small carbon clusters on Si and diamond surfaces and Al clusters on Ni substrate, were mainly discussed. The impact energy of the cluster ranges from 0.1 eV to 100 eV. In the former case, the mobility and the lateral migration of surface atoms, especially the recoil atoms, are enhanced with increasing the impact energy, which promote the film to be smoother and denser. For the latter case, the transverse kinetic energy of cluster atoms, caused mainly by the collision between moving cluster atoms, dominates the lateral spread of cluster atoms on the surface, which is contributive to layer-by-layer growth of thin films. Our result is consistent with the experimental observations that the film structure is strongly dependent on the impact energy. In addition, it elucidates that the atomic mobility takes a leading role in the structure characteristic of films formed by LECBD. 展开更多
关键词 薄膜生长 纳米结构 原子迁移率 能量丛聚 分子力学仿真
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NONSTABLE GROWTH OF Al-Si EUTECTIC IN CONSTRAINED CONDITION
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作者 LIU Junming ZHOU Yaohe SHANG Baolu Northwestern Polytechnical University,Xi’an,China LIU Junming P.O.Box No.219,Northwestern Polytechnical University,Xi’an 710072,China 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 1990年第9期166-171,共6页
Nonstable growth of Al-Si eutectic in constrained condition has been investigated by means of change of the withdrawing rate in an unidirectional solidification system,In constant acceleration growth,a retarded effect... Nonstable growth of Al-Si eutectic in constrained condition has been investigated by means of change of the withdrawing rate in an unidirectional solidification system,In constant acceleration growth,a retarded effect of response of eutectic interflake spacing on the growth rate has been observed.The response mechanisms are cluster-branching and local termination extension of Si-phase.In constant deceleration growth,the cluster-branching and termina- tion mechanisms of silicon still control adjustment of the interflake spacing,but the spacing is determined by the initial growth rate and does not respond to the decreasing growth rate. 展开更多
关键词 Al-Si eutectic nonstable growth Si-phase cluster-branching
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Prediction of Criminal Suspects Based on Association Rules and Tag Clustering
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作者 Bo Cheng Weihong Li Haoxin Tong 《Journal of Software Engineering and Applications》 2019年第3期35-50,共16页
To date, not many studies have been conducted on criminal prediction. In this study, the criminal data related to city S is divided into a training data set and a validation data set at a 1:1 ratio in light of the per... To date, not many studies have been conducted on criminal prediction. In this study, the criminal data related to city S is divided into a training data set and a validation data set at a 1:1 ratio in light of the personal tag data and the travel and accommodation data of criminals and ordinary people in city S. Firstly, the FP-growth algorithm is adopted to calculate association rules between the criminals and the ordinary people in their travel and hotel accommodation data, in order to discover criminal suspects based on association rules. Secondly, the DBSCAN algorithm is employed for clustering of the tag data of the criminals and the ordinary people, followed by similarity calculation, in order to discover criminal suspects based on tag clustering. Lastly, intersection operation is performed on the above two sets of criminal suspects, and the resulting intersection is verified against the criminal validation set for elimination of criminals who appear in the intersection so as to obtain final criminal suspects. Results show that a set of 648 criminal suspects is retrieved based on the association rules calculated by the FP-growth algorithm, while a set of 973 criminal suspects is retrieved based on DBSCAN clustering and cosine similarity of the personal tags;the number of criminal suspects is narrowed down to 567 after the intersection operation of the two sets, and 419 of the 567 criminal suspects are further verified to be criminals using the validation set, thereby leaving the other 148 to be the final criminal suspects and giving a prediction accuracy of 73.9%. The data mining method of criminal suspects based on association rules and tag clustering in this study has been successfully applied to the police system of city S, and the experiment proves the effectiveness of this method in detecting criminal suspects. 展开更多
关键词 FP-growth Association Rule DBSCAN TAG clusterING CRIMINAL Suspects
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25个奇楠沉香无性系生长与光合生理特征分析 被引量:2
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作者 张启雷 刘小金 +5 位作者 李小飞 方小英 徐大平 朱士铭 钟锦祥 熊咏梅 《广西林业科学》 2024年第3期295-301,共7页
为筛选适合广东惠州地区推广和种植的奇楠沉香(Aquilaria sinensis'Qi Nan')优良无性系,以25个奇楠沉香无性系为研究材料,分析不同无性系间生长性状和光合生理差异及其相关性,并进行聚类分析。结果表明,不同奇楠沉香无性系的保... 为筛选适合广东惠州地区推广和种植的奇楠沉香(Aquilaria sinensis'Qi Nan')优良无性系,以25个奇楠沉香无性系为研究材料,分析不同无性系间生长性状和光合生理差异及其相关性,并进行聚类分析。结果表明,不同奇楠沉香无性系的保存率、苗高、地径、冠幅和光合生理参数均差异显著;其中,保存率为22.7%~95.0%,苗高为117~210 cm,地径为16.8~34.3 mm。苗高与地径呈极显著正相关,与保存率呈显著正相关。聚类分析将25个无性系分为6类;第1类长势较好,保存率、苗高和地径分别为91.7%~95.0%、192~194 cm和28.1~30.9 mm,包括无性系Diao YW、Ao S和Lan BS;第2类苗高较高(199~210 cm),包括无性系Jin SZ、Qi NYH、Jin SY和Zhi TJ;第3类保存率较高(80.1%~93.7%),包括无性系Jin ShaY、Pu TT、Tu YW、Xiang SYH、Da YP和Hei ZZ;其他类群的保存率、苗高和地径均较低。无性系Diao YW、Ao S和Lan BS在惠州地区生长速度快且保存率高,适宜作为优良无性系进行推广。 展开更多
关键词 无性系 生长 光合生理 聚类分析 奇楠沉香
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过表达lncRNA HAGLR促胫骨骨折大鼠骨髓间充质干细胞成骨分化的机制研究
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作者 王文 陈新宇 +2 位作者 黄兹艺 邓杨柳 崔红旺 《局解手术学杂志》 2024年第6期472-478,共7页
目的研究骨质疏松(OP)-胫骨骨折(TF)大鼠长链非编码RNA同源盒D基因簇反义生长相关长链非编码RNA(lncRNA HAGLR)与其下游靶基因的表达情况,并探讨lncRNA HAGLR对大鼠骨髓间充质干细胞(MSC)成骨分化的作用与机制。方法30只SD雌性大鼠随机... 目的研究骨质疏松(OP)-胫骨骨折(TF)大鼠长链非编码RNA同源盒D基因簇反义生长相关长链非编码RNA(lncRNA HAGLR)与其下游靶基因的表达情况,并探讨lncRNA HAGLR对大鼠骨髓间充质干细胞(MSC)成骨分化的作用与机制。方法30只SD雌性大鼠随机分为sham组、OP组、OP-TF组,每组10只。ELISA法检测大鼠血清碱性磷酸酶(ALP)和抗酒石酸酸性磷酸酶(TRAP)的水平。对大鼠MSC细胞系R7500使用成骨分化诱导培养基进行诱导,并分为MSC组和成骨诱导组(Osteogenic-MSC组)。分别转染pcDNA-HAGLR、pcDNA-NC、miR-19a-3p的模拟物(miR-19a-3p mimic)、mimic的阴性对照(NC mimic)、miR-19a-3p的抑制剂(miR-19a-3p inhibitor)、miR-19a-3p inhibitor的阴性对照(NC inhibitor)至R7500后进行相应分组。双荧光素酶报告基因实验验证lncRNA HAGLR和miR-19a-3p以及骨形态发生蛋白2(BMP2)和miR-19a-3p的靶向关系。qRT-PCR检测各组lncRNA HAGLR和miR-19a-3p的表达。Western blot检测BMP2、ALP、胶原蛋白I(COL-I)、骨钙素(OCN)、骨桥蛋白(OPN)的表达。ALP染色和AR染色检测MSC的成骨分化能力。结果OP组和OP-TF组的血清ALP和TRAP水平均高于sham组,差异有统计学意义(P<0.05)。而OP组与sham组胫骨组织中lncRNA HAGLR、miR-19a-3p、BMP2的表达水平比较差异均无统计学意义(P>0.05),而OP-TF组胫骨组织中lncRNA HAGLR、BMP2的表达水平均明显低于sham组和OP组(P<0.05),OP-TF组胫骨组织中miR-19a-3p的表达水平高于sham组和OP组(P<0.05)。与MSC组相比,Osteogenic-MSC组的lncRNA HAGLR表达水平明显升高(P<0.05),而miR-19a-3p的表达降低(P<0.05)。双荧光素酶报告基因实验表明lncRNA HAGLR与miR-19a-3p具有靶向关系,miR-19a-3p与BMP2具有靶向关系。pcDNA-HAGLR组的miR-19a-3p的表达水平低于pcDNA-NC组(P<0.05)。miR-19a-3p mimic组与NC mimic组的lncRNA HAGLR的表达水平比较,差异无统计学意义(P>0.05)。与NC mimic组相比,miR-19a-3p mimic组BMP2的表达水平降低(P<0.05),miR-19a-3p表达水平升高(P<0.05)。pcDNA-HAGLR组细胞较pcDNA-NC组具有更强的成骨分化能力和更高的ALP活性(P<0.05)。miR-19a-3p inhibitor组细胞较NC inhibitor组具有更强的成骨分化能力和更高的ALP活性(P<0.05)。结论胫骨骨折大鼠lncRNA HAGLR和BMP2表达降低,miR-19a-3p表达增高。过表达lncRNA HAGLR通过靶向调控miR-19a-3p/BMP2轴促进大鼠MSC的成骨分化。 展开更多
关键词 骨质疏松 胫骨骨折 长链非编码RNA同源盒D基因簇反义生长相关长链非编码RNA miR-19a-3p 骨形态发生蛋白2 成骨分化
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