芝麻过敏原Ses i 2核酸适体的筛选与鉴定研究

芝麻是八大类食物过敏原之一,快速准确识别芝麻过敏原对预防其过敏有重要意义。核酸适配体可以高效识别靶标过敏原,在过敏原检测中有良好的应用前景。为了获得芝麻主要过敏原Ses i 2的特异性核酸适体,本研究以Ses i 2为靶标,通过磁珠筛选法(磁珠-SELEX)开展10轮筛选,经由高通量测序获得6条候补序列(S1~S6),并进行家族性、同源性分析及二级结构预测。结果表明,6条候选核酸适体的重复率可达46.38%,其自由能在-9.02到-2.47 kcal·moL^(-1)之间,根据自由能能量稳定原则,S1和S5吉布斯自由能最低最稳定,分别为-6.70和-9.02 kcal·moL^(-1)。利用ELISA试验进行亲和力测试,结果表明核酸适体S1和S2的亲和能力较强,S1:KD=67.02 nmol·L^(-1),R2=0.925 8,S2:KD=97.65 nmol·L^(-1),R2=0.795 1。核酸适体S1与过敏原Ses i 2的结合力和其他过敏原蛋白相比有显著差异,可视为具有特异性。本研究最终获得一条兼具良好亲和力和特异性的核酸适体S1,为芝麻过敏原快速检测提供了技术支撑。

S空位及非金属掺杂单层Bi_(2)S_(2)Se的第一性原理研究

Bi_(2)S_(2)Se是一种新兴的具有优异光电性能的材料,因此受到了广泛的关注.本研究采用第一性原理方法和开源程序,研究了本征单层Bi_(2)S_(2)Se以及掺杂非金属元素X(X=P,Cl,Br和I)和S空位的体系的电子结构和光学特性.计算结果表明,改性后出现了晶格缺陷.含S空位和P、Cl、Br掺杂体系的带隙减少,而I掺杂体系的带隙增加.同时,含S空位和Cl、Br、I掺杂体系的费米能级穿过导带底,而P掺杂体系的费米能级穿过价带顶.所有改性体系在费米能级附近都出现了缺陷能级或杂质能级,表现出较高的可见光吸收能力和散射能力,改性体系的对可见光的吸收能力和散射能力有所增加.

Mediating Effect of Mindfulness,Self-Esteem and Psychological Resilience in the Relation between Childhood Maltreatment and Life Satisfaction

Childhood maltreatment,as a typical early adverse environment,is known to have a negative impact on one’s life satisfaction.Mindfulness,on the other hand,may serve as a protective factor.This study explored the mediating role of mindfulness and its related variables–positive thoughts,psychological resilience and self-esteem.In order to testify the mechanism,we administered Childhood Trauma Questionnaire(CTQ),Satisfaction with Life Scale(SWLS),Mindful Attention Awareness Scale(MAAS),Connor–Davidson Resilience Scale(CD-RISC)and Rosenberg Self-Esteem Scale(RSES)to a non-clinical sample of Chinese university students(N=1021).The results indicated that positive thoughts did not mediate the relationship between childhood maltreatment and life satisfaction,but self-esteem(β=−0.194,95%CI=[−0.090,−0.040])and psychological resilience(β=−0.063,95%CI=[−0.059,−0.020])mediated the relationship,as well as the“mindfulness-selfesteem”(β=−0.061,95%CI=[−0.287,−0.126])and“mindfulness-psychological resilience”(β=−0.035,95%CI=[−0.115,−0.034]).The results of this study were helpful to understand the relationship between childhood maltreatment and life satisfaction and provided a theoretical basis for the development of mindfulness intervention programs from the perspective of positive psychology.

The Influence of Vulnerable Narcissism on Social Anxiety among Adolescents:The Mediating Role of Self-Concept Clarity and Self-Esteem

Social anxiety (SA) is a prevalent mental health issue among adolescents, and vulnerable narcissism (VN) can exacerbate thiscondition. This study aims to investigate the impact of vulnerable narcissism on social anxiety in adolescents, specificallyfocusing on the mediating effects of self-concept clarity (SCC) and self-esteem (SE) in the relationship between vulnerablenarcissism and social anxiety. Through cluster sampling, a questionnaire survey was conducted among 982 students from threesecondary schools in two provinces. The data was analyzed using structural equation modeling (SEM). The results revealedthat there was a significant negative correlation between vulnerable narcissism and both self-concept clarity and self-esteem,while there was a significant positive correlation between vulnerable narcissism and social anxiety. Additionally, self-conceptclarity showed a significant positive correlation with self-esteem but had a negative correlation with social anxiety. Both selfconceptclarity and self-esteem played an intermediary role in the chain linking vulnerable narcissism to social anxiety. Thisstudy confirms the mediating role of both self-concept clarity and self-esteem in explaining how vulnerable narcissisminfluences social anxiety, providing valuable insights into its underlying mechanism.

Self-Compassion Moderates the Effect of Contingent Self-Esteem on Well-Being:Evidence from Cross-Sectional Survey and Experiment

Contingent self-esteem captures the fragile nature of self-esteem and is often regarded as suboptimal to psychological functioning.Self-compassion is another important self-related concept assumed to promote mental health and well-being.However,research on the relation of self-compassion to contingent self-esteem is lacking.Two studies were conducted to explore the role of selfcompassion,either as a personal characteristic or an induced mindset,in influencing the effects of contingent self-esteem on well-being.Study 1 recruited 256 Chinese college students(30.4%male,mean age=21.72 years)who filled out measures of contingent self-esteem,self-compassion,and well-being.The results found that self-compassion moderated the effect of contingent self-esteem on well-being.In Study 2,a sample of 90 Chinese college students(34%male,mean age=18.39 years)were randomly assigned to either a control or self-compassion group.They completed baseline trait measures of contingent self-esteem,self-compassion,and self-esteem.Then,they were led to have a 12-min break(control group)or listen to a 12-min self-compassion audio(self-compassion group),followed by a social stress task and outcome measures.The results demonstrated the effectiveness of the brief self-compassion training and its moderating role in influencing the effects of contingent self-esteem on negative affects after the social stress task.This research provides implications that to equip with a self-compassionate mindset could lower the risk of the impairment of well-being associated with elements of contingent selfesteem,which involves a fragile sense of self-worth.It may also provide insights into the development of an“optimal selfesteem”and the improvement of well-being.

Disparities in the impact of economic well-being on self-esteem in adulthood:Race and ethnicity

BACKGROUND Most studies have defined economic well-being as socioeconomic status,with little attention given to whether other indicators influence self-esteem.Little is known about racial/ethnic disparities in the relationship between economic wellbeing and self-esteem during adulthood.AIM To explore the impact of economic well-being on self-esteem in adulthood and differences in the association across race/ethnicity.METHODS The current study used data from the National Longitudinal Survey of Youth 1979.The final sample consisted of 2267 African Americans,1425 Hispanics,and 3678 non-Hispanic Whites.Ordinary linear regression analyses and logistic regression analyses were conducted.RESULTS African Americans and Hispanics were more likely to be in poverty in comparison with non-Hispanic Whites.More African Americans were unemployed than Whites.Those who received fringe benefits,were more satisfied with jobs,and were employed were more likely to have higher levels of self-esteem.Poverty was negatively associated with self-esteem.Interaction effects were found between African Americans and job satisfaction predicting self-esteem.CONCLUSION The role of employers is important in cultivating employees’self-esteem.Satisfactory outcomes or feelings of happiness from the workplace may be more important to non-Hispanic Whites compared to African Americans and Hispanics.

The Relationship between Self-Esteem and Social Phobia among College Students:The Mediating Effect of Fear of Negative Evaluation and the Moderating Effect of Perfectionism

Objectives:To explore the relationship between college students’self-esteem(SE)and their social phobia(SP),as well as the mediating role of fear of negative evaluation(FNE)and the moderating effect of perfectionism.Methods:A convenience sampling survey was carried out for 1020 college students from Shandong Province of China,utilizing measures of college students’self-esteem,fear of negative evaluation,perfectionism,and social phobia.Data analysis was performed using the SPSS PROCESS macro.Results:(1)college students’self-esteem significantly and negatively predicts their social phobia(β=−0.31,t=−10.10,p<0.001);(2)fear of negative evaluation partially mediates the relation between self-esteem and social phobia among college students,with the mediating effect accounting for 48.97%of the total effect(TE);(3)the mediating role of fear of negative evaluation is moderated by perfectionism(β=0.18,t=7.75,p<0.001),where higher levels of perfectionism strengthen the mediating effect of fear of negative evaluation.Conclusions:Perfectionism moderates the mediating effect that fear of negative evaluation plays,establishing a moderated mediating model.

Leadership Virtues, Professional Self-Esteem, and Teaching Management of Chinese University Professors: Basis for Professional Development Plan

This study used a descriptive-quantitative research method as it describes the profile of Chinese university professors in terms of age,gender,and educational background.It also described the leadership virtues in terms of prudence,fortitude,temperance,and justice;professional self-esteem in terms of career choice and adaptability,career satisfaction and values,career impact and fulfillment,and career competence and evaluation;and teaching management in terms of teaching management and evaluation,the area of teaching and research activities,and the area of collective lesson planning activities.Diversity leadership training and instructional management training are provided to meet the needs of different groups of faculty and staff of different gender,age,and education levels.Individualized professional development plans are based on the personal characteristics and needs of professors,particularly in the areas of leadership,teaching management,and career satisfaction.To address the relationship among leadership virtues,professional self-esteem,and teaching management,long-term,in-depth research is encouraged to better understand the dynamics between them.Based on the results,this paper recommends the implementation of the proposed professional development plan.

中国文化的自尊与西方文化的self-esteem之比较

self-esteem从西方心理学界引进而来,中国心理学界一直将之翻译成"自尊"并在此基础上进行研究,但中国文化中的"自尊"与self-esteem在多个维度上存在着不同。Self-esteem指的是个体对自己的认识、评价或者情感,具体表现为自我胜任感、自我价值感或者这二者的结合。中国文化中的"自尊"包括个体自尊、民族自尊和文化自尊。"个体自尊"指的是尊重自己,不向别人卑躬屈膝,也不容许别人歧视、侮辱;"民族自尊"指的是捍卫自己民族的尊严,抵御外侮;"文化自尊"指的是尊重和维护自己民族的文化,保持自己民族的特色,不可以盲目崇拜其他民族文化,要发扬自己民族文化的优秀传统。中国本土文化中的"自尊"在涉及的内容、情境、应对方式等方面都和self-esteem存在不同。

基于CNN‑LSTM‑SE的心电图分类算法研究

心血管疾病是我国死亡率较高的疾病之一,通过观察心电图来判断心电信号是否出现异常能够对心血管疾病进行预防和筛查。由于心电图数据规模大且繁杂,临床医护人员在心电图筛查时,工作负担大且容易出现误诊或漏诊的情况。为了提高心电图的筛查效率、减少医护人员的压力,提出了一种基于卷积神经网络、长短期记忆神经网络和SE网络的心电图分类算法模型(CNN-LSTM-SE),该模型将心电图分成5种不同的类别。主要研究内容包括:选用MIT-BIH心律失常数据集作为心电信号的数据来源,使用巴特沃斯带通滤波器对心电信号进行去噪处理,通过Z-score方法对心电信号进行标准化处理,利用独热编码方法对心电信号标签进行编码,最后使用处理后的心电数据对所提算法模型进行训练和测试。实验结果表明:所提模型相较于其它模型,能够有效提高心电图分类的准确性,在实验数据集上的分类准确率达到99.1%。

重金属地质高背景区土壤Se的地球化学特征及富Se耕地综合评价

为了合理保护和利用富Se耕地资源,为重金属地质高背景区富Se农产品的安全开发提供科学依据,以重庆市黔江区白土乡为例,采集74份耕地土壤(0~20 cm)及水稻籽实样品,分析稻谷及土壤中Se和重金属(Cd、Hg、Pb、As、Cr、Cu、Zn和Ni)含量,同时分析土壤中养分元素及氧化物含量,研究土壤及稻谷中各元素的地球化学特征,并开展富Se耕地综合评价。结果显示,研究区土壤环境综合质量等级以三等(轻度污染)和四等(中度污染)为主,面积占比分别为41.65%和50.71%,污染因子为Cd。土壤Se平均含量为0.91 mg·kg^(-1),含量介于0.4~3.0 mg·kg^(-1)之间的点位占比为97.29%,土壤Se资源丰富。研究区耕地土壤总体养分条件良好,但部分区域存在单元素缺乏问题,以K缺乏最为显著。耕地土壤重金属和Se含量主要与灰岩、炭质页岩(夹煤线)的风化成土有关。此外,农业活动会造成耕地土壤Cd和As的输入。研究区糙米富Se率为100%,糙米Cd超标率为7.4%,土壤pH值是影响稻谷吸收土壤Cd和Se的主控因素,中碱性条件有利于水稻富集Se而抑制其对Cd的吸收。研究区富Se耕地综合等级以二等为主,面积占比为95.68%,适宜开展富Se资源开发利用。可根据各耕地地块单项指标得分情况,通过养分元素补充、土壤酸碱度调节等措施,趋利避害利用土壤富Se资源,对部分不适宜开展富Se资源开发利用的耕地,可进行种植结构调整。

A Study of the Relationship between Advanced Vocational College Students' Self-esteem and Their English Achievements

Self-esteem is one of personality traits in the process of language learning.There is a close relationship between the level of self-esteem and language learning achievements.The purpose of the study is to discuss the relationship between EFL self-esteem and English achievements in the process of their English learning.The study also shows that EFL self-esteem can influence English achievements.On the other hand,English achievements can influence EFL self-esteem too.Based on the research,some methods are proposed to help strengthen students' EFL self-esteem and improve their English achievement.

Implication of Self-esteem for Language Teaching from Neurobiological Perspective

In the past several decades,the cognitive skills in language learning have been put more attention to than emo-tions.While it should be noted that language learning often involves strong positive or negative emotions.Lack of positive emotionin language learning will lead to severe discount of the cognitive competence.This article is intended to discuss the influence ofemotion,especially self-esteem,on motivation in language learning from Schumann’s neurobiological perspective,and the implica-tion of self-esteem for language teaching—how to enhance learners’self-esteem,to promote their motivation,hence to guidethem to step into their next zone of proximal development in both academic performance and social skills and become whole per-sons responsible for our society.

基于改进SE-Net和深度可分离残差的高光谱图像分类

针对目前常见的用于高光谱图像分类的卷积神经网络参数数量多,训练时间长,对样本数量依赖性大的问题,提出一种适用于有限训练样本条件下基于改进压缩激活网络和深度可分离残差的分类网络MDSR&SE-Net.首先使用主成分分析对原始高光谱图像进行通道降维,然后通过三维卷积神经网络连接多特征残差结构,同时嵌入改进的SE模块提取高光谱图像的空间和光谱细节特征,最后将提取到的特征数据输入Softmax分类器激活分类.为了使网络更加轻量,通过在残差结构中使用深度可分离卷积和引入全局平均池化减少参数数量.实验结果显示,使用有限训练样本在三种常见高光谱数据集上总体分类精度均达到99%以上.

The relationships among perceived organizational support,intention to remain,career success and self-esteem in Chinese male nurses

Aim:The purpose of this study was to investigate the relationships among perceived organizational support,intention to remain,career success and self-esteem in male nurses in China.Background:Recently,turnover is considered increasingly more normal.Male nurses could be stabilized by improving their perceived organizational support,career success and selfesteem.Design:A cross-sectional design was used in the present study.Method:405 full-time male Registered Nurses,from Grade A hospitals in Tianjin,were surveyed.Pearson correlation,Cronbach's alpha coefficient and structural equation modelling were conducted in the study.Results:The results illustrated that self-esteem mediated the relationship between perceived organizational support and career success.Career success mediated the relationship between self-esteem and intention to remain.Conclusion:Hospitals should pay more attention to the benefits of positive personality trait on working motivation.It is meaningful to enhance perceived organizational support,career success and self-esteem in the working process so that nurses are willing to stay in the current employment.

基于SE注意力CycleGAN的蓝印花布单纹样自动生成

根据蓝印花布纹样的风格特征,文章提出一种端到端的蓝印花布纹样自动生成方法,实现简笔画图像向蓝印花布单纹样的自动迁移。针对蓝印花布的抽象风格和小数据集问题,重新构造CycleGAN生成网络中的编码器和解码器,使用SE(squeeze and excitation)注意力模块和残差模块与原始的卷积模块串联,提高特征提取能力和网络学习能力。同时减少生成网络中转换器的残差块层数,降低过拟合。实验结果表明,基于SE注意力CycleGAN网络方法自动生成的蓝印花布新纹样主观性上更贴合原始风格,与原图更加接近,有助于蓝印花布的数字化传承和创新。

A research regarding the relationship among intensive care nurses' self-esteem, job satisfaction and subjective well-being

Objective:The paper aims to explore the status of intensive care unit(ICU)nurses'self-esteem,job satisfaction,and subjective well-being and to investigate the relationship among the three elements.Methods:A cross-sectional survey of 224 nurses from five teaching hospitals in Tianjin,China,was conducted.Self-esteem scale,job satisfaction scale,and subjective well-being scale were used to explore the status of ICU nurses'self-esteem,job satisfaction,and subjective well-being.Correlation among the three elements was calculated by SPSS 17.0.Results:The scores of three scales(i.e.,ICU nurses'self-esteem,job satisfaction,and subjective well-being)were 27.67±3.41,43.57±9.04,and 69.73±11.60,respectively.The subjective well-being was significantly positively correlated with self-esteem and job satisfaction(r=0.454,P<0.01;r=0.584,P<0.01,respectively).Conclusion:The study shows that the prevalence of subjective well-being and self-esteem with ICU nurses are at a low level,and job satisfaction is at the median level.Associations among self-esteem,job satisfaction,and subjective well-being in ICUs have been confirmed.Self-esteem and job satisfaction positively correlated with nurse subjective well-being.The higher the self-esteem and job satisfaction levels are,the stronger the subjective well-being is.The working pressure in ICUs cannot be alleviated temporarily;hence,to promote a high subjective well-being,managers and ICU nurses increase their self-esteem and job satisfaction through taking strategies that strengthen psychological construction.

基于DEMATEL和SE-DEA的民航监察员培训效能评价

为优化培训模式,提高监察员的安全监管能力,降低民航各种不安全事件发生的概率,以“岗位胜任能力导向”为核心的培训模式逐步形成。首先基于“一个素质,三个能力”构建初始岗位胜任力模型,然后结合SPSS和AMOS进行指标筛选和模型验证,利用DEMATEL法探究各指标之间的影响关系并改进权重,最后运用SE-DEA法检验目前的培训课程设置是否合理并进行评价。结果表明:构建的岗位胜任力共15项指标,具有良好的效度和信度;政治素质和管理能力对法治能力和业务能力的影响更大,对胜任力模型影响最大的是业务能力;经过培训,75%的监察员的岗位能力都得到有效提升,达到强化基层安全能力建设的预期,同时说明目前的培训课程设置仍需进一步改进。

The Effects of a Single Bout of Exercise on Mood and Self-Esteem in Clinically Diagnosed Mental Health Patients

Objectives: Research has highlighted the importance of regular exercise within the general population and mental health groups in regard to mood and self-esteem, as well as single bout exercise within the general population. However, research into single bout exercise in mental health population is lacking. This study investigated the impact of a single bout of exercise, on mood and self-esteem, in patients with a wider clinical mental health diagnosis. Design: A quantitative questionnaire was completed immediately pre and post a single, 45 minute bout of moderate intensity exercise, consisting of the Brunel Universal Mood States (BRUMS) questionnaire and the Rosenberg Self Esteem Scale (RSE). Methods: Participants attending a mental health hospital with a clinical mental health diagnosis (N = 54) completed the questionnaire. Information regarding physical activity levels, mental health diagnosis and length of hospital stay were collated. Results: A significant improvement was identified on the RSE as well as the BRUMS (depression, anger, confusion, anxious tension and vigour) over time. Conclusion: The significant findings highlight the importance of exercise promotion within this population group, and the potentially beneficial role that a single bout of exercise can have on mood and self-esteem in patients experiencing mental health problems.

Meaning in life and well-being of older stroke survivors in Chinese communities: Mediating effects of mastery and self-esteem

The study aims to examine the role of mastery and self-esteem as a potential mediator of the relationship of meaning in life and well-being among older Chinese stroke survivors. A cross-sectional study was conducted in 214 community-dwelling older stroke survivors (128 men and 86 women), ranging from 60 years to 88 years old. The meaning in life, mastery, self-esteem and subjective well-being were measured. The results indicated that: 1) The meaning in life and subjective well-being of stroke survivors were significantly positive correlated;2) Mastery and self-esteem played partial mediating roles between existential vacuum and subjective well-being;Self-esteem played full a mediating role between suffer acceptance and subjective well-being;Mastery played a full mediating role between life control and subjective well-being;Mastery played a partial mediating role between death acceptance and subjective well-being;Self-esteem also played a partial mediating role between mastery and subjective well-being.