Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45S5 bioactive glass(45S5 BG)is a promising material because...Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45S5 bioactive glass(45S5 BG)is a promising material because of its osteoconductive and bioactive properties.As research in this field continues to advance,keeping up-to-date on the latest and most successful applications of this material is imperative.To achieve this,we conducted a comprehensive search on Pub-Med/MEDLINE,focusing on English articles published in the last decade.Our search used the keywords“bioglass 45S5 AND bone defect”in combination.We found 27 articles,and after applying the inclusion criteria,we selected 15 studies for detailed examination.Most of these studies compared 45S5 BG with other cement or scaffold materials.These comparisons demonstrate that the addition of various composites enhances cellular biocompatibility,as evidenced by the cells and their osteogenic potential.Moreover,the use of 45S5 BG is enhanced by its antimicrobial properties,opening avenues for additional investigations and applications of this biomaterial.展开更多
目的(1)探究锂基生物玻璃水凝胶,赋予介孔生物玻璃材料促成骨、促血管生成和抑制脂肪生成等多重生物医学功能。(2)利用3D打印技术制作导航模板,精确定位股骨头坏死病灶区域和清除病灶,将锂基生物玻璃水凝胶精确注入,观察其修复股骨头坏...目的(1)探究锂基生物玻璃水凝胶,赋予介孔生物玻璃材料促成骨、促血管生成和抑制脂肪生成等多重生物医学功能。(2)利用3D打印技术制作导航模板,精确定位股骨头坏死病灶区域和清除病灶,将锂基生物玻璃水凝胶精确注入,观察其修复股骨头坏死的作用。方法利用明胶和甲基丙烯酸酐制备了甲基丙烯酰化明胶(gelatin methacryloyl,GelMA)及锂基生物玻璃水凝胶(GelMA and Li-mesoporous bioglass,GM/M-Li)。扫描电子显微镜(scanning electron microscope,SEM)、透射电子显微镜(transmission electron microscope,TEM)对材料进行表征分析;同时,通过细胞活/死染色、细胞增殖实验(cell counting kit-8,CCK-8)及细胞形态观察评估材料的生物相容性;另外,使用免疫荧光染色、碱性磷酸酯酶染色、茜素红染色、成血管实验、油红O染色评估材料的成骨分化能力、成血管能力和抑制脂肪生成的能力。体内成功建立了兔股骨头坏死模型,利用3D导航模板联合髓芯减压术将坏死骨去除后填充GM/M-Li水凝胶。通过苏木素伊红(hematoxylin eosin,HE)染色、油红O染色、免疫组化染色验证其成骨、成血管和抑制脂肪生成能力。结果SEM结果显示GM/M-Li水凝胶材料为三维多孔的结构;TEM结果表明,M-Li材料为纳米级别;离子释放实验证明了GM/M-Li水凝胶中的锂元素,同时具有缓释作用;细胞活/死染色、CCK-8证明了GM/M-Li水凝胶材料具有良好的生物相容性;体外成骨、成血管、抑制脂肪生成实验证实,材料中锂离子的释放有利于骨髓间充质干细胞的成骨分化、人脐静脉血管内皮细胞的小管形成,抑制了脂肪前体细胞的脂肪形成。体内HE染色和免疫荧光验证了GM/M-Li水凝胶有效促进了骨组织、血管再生;通过油红O染色验证了GM/M-Li水凝胶的抑制脂肪能力。结论利用3D打印技术制作导航模板,将GM/M-Li精确注入股骨头坏死病灶区域和清除病灶。因GM/M-Li水凝胶具有良好的生物相容性,其可通过调节骨髓间充质干细胞成骨方向分化,促进股骨头坏死中骨缺损的重建,同时促进了血管的生成,减少脂肪细胞的浸润。展开更多
Tooth bleaching agents may weaken the tooth structure. Therefore, it is important to minimize any risks of tooth hard tissue damage caused by bleaching agents. The aim of this study was to evaluate the effects of appl...Tooth bleaching agents may weaken the tooth structure. Therefore, it is important to minimize any risks of tooth hard tissue damage caused by bleaching agents. The aim of this study was to evaluate the effects of applying 45S5 bioglass （BG） before, after, and during 35% hydrogen peroxide （HP） bleaching on whitening efficacy, physicochemical properties and microstructures of bovine enamel. Seventy-two bovine enamel blocks were prepared and randomly divided into six groups： distilled deionized water （DDW）, BG, HP, BG before HP, BG after HP and BG during HP. Colorimetric and microhardness tests were performed before and after the treatment procedure. Representative specimens from each group were selected for morphology investigation after the final tests. A significant color change was observed in group HP, BG before HP, BG after HP and BG during HP. The microhardness loss was in the following order： group HP〉 BG before HP, BG after HP〉 BG during HP〉DDW, BG. The most obvious morphological alteration of was observed on enamel surfaces in group HP, and a slight morphological alteration was also detected in group BG before HP and BG after HP. Our findings suggest that the combination use of BG and HP could not impede the tooth whitening efficacy. Using BG during HP brought better protective effect than pre/post-bleaching use of BG, as it could more effectively reduce the mineral loss as well as retain the surface integrity of enamel. BG may serve as a promising biomimetic adjunct for bleaching therapy to prevent/restore the enamel damage induced by bleaching agents.展开更多
Mechanical alloying and annealing at 1150 °C for 2 h under an argon atmosphere were used to prepare Ti-45S5 bioglass nanocomposites. Ti-45S5 bioglass material was chemically modified by silver. The antibacterial ...Mechanical alloying and annealing at 1150 °C for 2 h under an argon atmosphere were used to prepare Ti-45S5 bioglass nanocomposites. Ti-45S5 bioglass material was chemically modified by silver. The antibacterial activity of Ti-10% 45S5 bioglass nanocomposite containing silver against Streptococcus mutans and Staphylococcus aureus was studied. Nanocomposites were characterized by X-ray diffraction, scanning electron microscopy equipped with an electron energy dispersive spectrometer and transmission electron microscopy to evaluate phase composition, crystal structure and grain size. In vitro bacterial adhesion study indicated a significantly reduced number of Streptococcus mutans and Staphylococcus aureus on the bulk nanostructured Ti-45S5 bioglass-Ag plate surface in comparison to that on microcrystalline Ti plate surface. Nanostructured Ti-based biomaterials can be considered to be the future generation of dental implants.展开更多
Objective: To study the regulating effect of borosilicate bioglass extract on the osteoblast proliferation activity and osteogenesis signaling pathway function. Methods: Osteoblasts MG-63 were cultured and divided int...Objective: To study the regulating effect of borosilicate bioglass extract on the osteoblast proliferation activity and osteogenesis signaling pathway function. Methods: Osteoblasts MG-63 were cultured and divided into borosilicate group and control group that were treated with the culture medium containing borosilicate bioglass extract and the culture medium without extract respectively. After 24 h of treatment, the cell proliferation activity as well as the expression of proliferation activity markers, Wnt signaling pathway molecules and PI3K/AKT signaling pathway molecules was measured. Results: After 24 h of treatment, MTT cell viability of borosilicate group was significantly higher than that of control group, and ALP, OC, OPN, COL-I, Runx2, Wnt1, Wnt3a, β-catenin, LRP5, LRP6, p-PI3K, p-AKT, Bcl-2 and BMP protein expression in cells were significantly higher than those of control group. Conclusion: Borosilicate bioglass extract can enhance the proliferation activity of osteoblasts by activating Wnt pathway and PI3K/AKT pathway.展开更多
Biodegradable magnesium(Mg) and its alloy show huge potential as temporary bone substitute due to the favorable biocompatibility and mechanical compatibility. However, one issue deserves attention is the too fast degr...Biodegradable magnesium(Mg) and its alloy show huge potential as temporary bone substitute due to the favorable biocompatibility and mechanical compatibility. However, one issue deserves attention is the too fast degradation. In this work, mesoporous bioglass(MBG)with high pore volume(0.59 cc/g) and huge specific surface area(110.78 m^(2)/g) was synthesized using improved sol-gel method, and introduced into Mg-based composite via laser additive manufacturing. Immersion tests showed that the incorporated MBG served as powerful adsorption sites, which promoted the in-situ deposition of apatite by successively adsorbing Ca2+and HPO42-. Such dense apatite film acted as an efficient protection layer and enhanced the corrosion resistance of Mg matrix, which was proved by the electrochemical impedance spectroscopy measurements. Thereby, Mg based composite showed a significantly decreased degradation rate of 0.31 mm/year. Furthermore,MBG also improved the mechanical properties as well as cell behavior. This work highlighted the advantages of MBG in the fabrication of Mg-based implant with enhanced overall performance for orthopedic application.展开更多
文摘Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45S5 bioactive glass(45S5 BG)is a promising material because of its osteoconductive and bioactive properties.As research in this field continues to advance,keeping up-to-date on the latest and most successful applications of this material is imperative.To achieve this,we conducted a comprehensive search on Pub-Med/MEDLINE,focusing on English articles published in the last decade.Our search used the keywords“bioglass 45S5 AND bone defect”in combination.We found 27 articles,and after applying the inclusion criteria,we selected 15 studies for detailed examination.Most of these studies compared 45S5 BG with other cement or scaffold materials.These comparisons demonstrate that the addition of various composites enhances cellular biocompatibility,as evidenced by the cells and their osteogenic potential.Moreover,the use of 45S5 BG is enhanced by its antimicrobial properties,opening avenues for additional investigations and applications of this biomaterial.
文摘目的(1)探究锂基生物玻璃水凝胶,赋予介孔生物玻璃材料促成骨、促血管生成和抑制脂肪生成等多重生物医学功能。(2)利用3D打印技术制作导航模板,精确定位股骨头坏死病灶区域和清除病灶,将锂基生物玻璃水凝胶精确注入,观察其修复股骨头坏死的作用。方法利用明胶和甲基丙烯酸酐制备了甲基丙烯酰化明胶(gelatin methacryloyl,GelMA)及锂基生物玻璃水凝胶(GelMA and Li-mesoporous bioglass,GM/M-Li)。扫描电子显微镜(scanning electron microscope,SEM)、透射电子显微镜(transmission electron microscope,TEM)对材料进行表征分析;同时,通过细胞活/死染色、细胞增殖实验(cell counting kit-8,CCK-8)及细胞形态观察评估材料的生物相容性;另外,使用免疫荧光染色、碱性磷酸酯酶染色、茜素红染色、成血管实验、油红O染色评估材料的成骨分化能力、成血管能力和抑制脂肪生成的能力。体内成功建立了兔股骨头坏死模型,利用3D导航模板联合髓芯减压术将坏死骨去除后填充GM/M-Li水凝胶。通过苏木素伊红(hematoxylin eosin,HE)染色、油红O染色、免疫组化染色验证其成骨、成血管和抑制脂肪生成能力。结果SEM结果显示GM/M-Li水凝胶材料为三维多孔的结构;TEM结果表明,M-Li材料为纳米级别;离子释放实验证明了GM/M-Li水凝胶中的锂元素,同时具有缓释作用;细胞活/死染色、CCK-8证明了GM/M-Li水凝胶材料具有良好的生物相容性;体外成骨、成血管、抑制脂肪生成实验证实,材料中锂离子的释放有利于骨髓间充质干细胞的成骨分化、人脐静脉血管内皮细胞的小管形成,抑制了脂肪前体细胞的脂肪形成。体内HE染色和免疫荧光验证了GM/M-Li水凝胶有效促进了骨组织、血管再生;通过油红O染色验证了GM/M-Li水凝胶的抑制脂肪能力。结论利用3D打印技术制作导航模板,将GM/M-Li精确注入股骨头坏死病灶区域和清除病灶。因GM/M-Li水凝胶具有良好的生物相容性,其可通过调节骨髓间充质干细胞成骨方向分化,促进股骨头坏死中骨缺损的重建,同时促进了血管的生成,减少脂肪细胞的浸润。
基金supported by the Research Fund from Science and Technology Department of Sichuan Province (No. 2009FZ0065)Key Project of the Science and Technology Department of Sichuan Province (No. 2011SZ0101)+1 种基金Doctoral Fund of Ministry of Education of China (No. 20120181120002)supported by Open Fund of State Key Laboratory of Oral Diseases, Sichuan University
文摘Tooth bleaching agents may weaken the tooth structure. Therefore, it is important to minimize any risks of tooth hard tissue damage caused by bleaching agents. The aim of this study was to evaluate the effects of applying 45S5 bioglass （BG） before, after, and during 35% hydrogen peroxide （HP） bleaching on whitening efficacy, physicochemical properties and microstructures of bovine enamel. Seventy-two bovine enamel blocks were prepared and randomly divided into six groups： distilled deionized water （DDW）, BG, HP, BG before HP, BG after HP and BG during HP. Colorimetric and microhardness tests were performed before and after the treatment procedure. Representative specimens from each group were selected for morphology investigation after the final tests. A significant color change was observed in group HP, BG before HP, BG after HP and BG during HP. The microhardness loss was in the following order： group HP〉 BG before HP, BG after HP〉 BG during HP〉DDW, BG. The most obvious morphological alteration of was observed on enamel surfaces in group HP, and a slight morphological alteration was also detected in group BG before HP and BG after HP. Our findings suggest that the combination use of BG and HP could not impede the tooth whitening efficacy. Using BG during HP brought better protective effect than pre/post-bleaching use of BG, as it could more effectively reduce the mineral loss as well as retain the surface integrity of enamel. BG may serve as a promising biomimetic adjunct for bleaching therapy to prevent/restore the enamel damage induced by bleaching agents.
文摘Mechanical alloying and annealing at 1150 °C for 2 h under an argon atmosphere were used to prepare Ti-45S5 bioglass nanocomposites. Ti-45S5 bioglass material was chemically modified by silver. The antibacterial activity of Ti-10% 45S5 bioglass nanocomposite containing silver against Streptococcus mutans and Staphylococcus aureus was studied. Nanocomposites were characterized by X-ray diffraction, scanning electron microscopy equipped with an electron energy dispersive spectrometer and transmission electron microscopy to evaluate phase composition, crystal structure and grain size. In vitro bacterial adhesion study indicated a significantly reduced number of Streptococcus mutans and Staphylococcus aureus on the bulk nanostructured Ti-45S5 bioglass-Ag plate surface in comparison to that on microcrystalline Ti plate surface. Nanostructured Ti-based biomaterials can be considered to be the future generation of dental implants.
文摘Objective: To study the regulating effect of borosilicate bioglass extract on the osteoblast proliferation activity and osteogenesis signaling pathway function. Methods: Osteoblasts MG-63 were cultured and divided into borosilicate group and control group that were treated with the culture medium containing borosilicate bioglass extract and the culture medium without extract respectively. After 24 h of treatment, the cell proliferation activity as well as the expression of proliferation activity markers, Wnt signaling pathway molecules and PI3K/AKT signaling pathway molecules was measured. Results: After 24 h of treatment, MTT cell viability of borosilicate group was significantly higher than that of control group, and ALP, OC, OPN, COL-I, Runx2, Wnt1, Wnt3a, β-catenin, LRP5, LRP6, p-PI3K, p-AKT, Bcl-2 and BMP protein expression in cells were significantly higher than those of control group. Conclusion: Borosilicate bioglass extract can enhance the proliferation activity of osteoblasts by activating Wnt pathway and PI3K/AKT pathway.
基金National Natural Science Foundation of China (51935014,52165043, 82072084, 81871498)Jiang Xi Provincial Natural Science Foundation of China (20192ACB20005,2020ACB214004)+6 种基金The Provincial Key R&D Projects of Jiangxi (20201BBE51012)Guangdong Province Higher Vocational Colleges&Schools Pearl River Scholar Funded Scheme (2018)Shenzhen Science and Technology Plan Project (JCYJ20170817112445033)Innovation Team Project on University of Guangdong Province(2018GKCXTD001)Technology Innovation Platform Project of Shenzhen Institute of Information Technology 2020(PT2020E002)China Postdoctoral Science Foundation(2020M682114)Open Research Fund of Jiangsu Key Laboratory of Precision and Micro-Manufacturing Technology。
文摘Biodegradable magnesium(Mg) and its alloy show huge potential as temporary bone substitute due to the favorable biocompatibility and mechanical compatibility. However, one issue deserves attention is the too fast degradation. In this work, mesoporous bioglass(MBG)with high pore volume(0.59 cc/g) and huge specific surface area(110.78 m^(2)/g) was synthesized using improved sol-gel method, and introduced into Mg-based composite via laser additive manufacturing. Immersion tests showed that the incorporated MBG served as powerful adsorption sites, which promoted the in-situ deposition of apatite by successively adsorbing Ca2+and HPO42-. Such dense apatite film acted as an efficient protection layer and enhanced the corrosion resistance of Mg matrix, which was proved by the electrochemical impedance spectroscopy measurements. Thereby, Mg based composite showed a significantly decreased degradation rate of 0.31 mm/year. Furthermore,MBG also improved the mechanical properties as well as cell behavior. This work highlighted the advantages of MBG in the fabrication of Mg-based implant with enhanced overall performance for orthopedic application.
