Membrane proteins are vital components of the cell membrane and play crucial roles in various cellular activities.Analysis of membrane proteins is of paramount importance for studying molecular events inside cells and...Membrane proteins are vital components of the cell membrane and play crucial roles in various cellular activities.Analysis of membrane proteins is of paramount importance for studying molecular events inside cells and organisms and holds promising prospects for early disease diagnosis and treatment assessment.Benefiting from obvious merits including high affinity,high specificity and ease of modification,aptamers have been regarded as ideal molecular recognition elements in membrane protein analysis and molecular diagnostics strategies.This review summarised recent advances in membrane protein-specific aptamer screening,aptamer-based static and dynamic membrane protein analysis,and aptamer-based molecular diagnostic techniques.Prospects and challenges were also discussed.展开更多
Recent developments in DNA nanotechnology have brought various nanoscale structures,devices and functional systems for different applications.As biological barriers with significant functions,cell membranes proide dir...Recent developments in DNA nanotechnology have brought various nanoscale structures,devices and functional systems for different applications.As biological barriers with significant functions,cell membranes proide direct interfaces for studying cellular environment and states.So far,DNA nanotechnology engineered on live cell membranes has advanced our fundamental understandings of DNA nanomaterials and facilitated the designs of novel sensing,imaging and therapeutic platforms.In this review,we highlighted strategies and outcomes of using DNA nanotechnology on cell membranes towards various biomedical applications,including biosensing,imaging,cellular function regulations and targeted cancer therapy.Furthermore,we also discussed the challenges and opportunities of DNA nanotechnology on cell membranes towards broader applications.展开更多
The invasion of etched dentinal tubules(DTs)by external substances induces dentin hypersensitivity(DH).The deep and compact occlusion of DTs is highly desirable for treating DH but still challenging due to the limited...The invasion of etched dentinal tubules(DTs)by external substances induces dentin hypersensitivity(DH).The deep and compact occlusion of DTs is highly desirable for treating DH but still challenging due to the limited penetrability and mineralization capacities of most current desensitizers.Matrix vesicles(MVs)participate in the regulation of ectopic mineralization.Herein,ectopic MV analogs are prepared by employing natural cell membranes to endow mineral precursors with natural biointerfaces and integrated biofunctions for stimulating dentin remineralization.The analogs quickly access DTs(>20μm)in only 5 min and further penetrate deep into the interior of DTs(an extraordinary~200μm)in 7 days.Both in vitro and in vivo studies confirm that the DTs are efficiently sealed by the newly formed minerals(>50μm)with excellent resistance to wear and acid erosion,which is significantly deeper than most reported values.After repair,the microhardness of the damaged dentin can be recovered to those of healthy dentin.For the first time,cell membrane coating nanotechnology is used as a facile and efficient therapy for in-depth remineralization of DTs in treating DH with thorough and long-term effects,which provides insights into their potential for hard tissue repair.展开更多
Filtration and membrane separation are popular methods in gas separation since they are cost and energy efficient. Despite to air filters, there are comparatively few studies on dry gas filters, particularly at indust...Filtration and membrane separation are popular methods in gas separation since they are cost and energy efficient. Despite to air filters, there are comparatively few studies on dry gas filters, particularly at industrial scale. In fact, major unsolved challenges such as high efficiency, low pressure drop, long-term stability, high-thermal and chemical stability and advanced physiochemical properties, are still remained. The aim of this review is to scrutinize the advanced scientific and technological practices (such as selection of appropriate polymeric materials and additives, nanotechnology, modification techniques and preparation methods) towards design and fabrication of an efficient filter media for solid particles removal from the natural gas flow. Recent progresses in solid particle separation mechanisms, modeling and simulation techniques and the effect of membrane fabrication methods on its performance, strategies for modification of filter media, current challenges and future perspective are discussed.展开更多
Bacterial outer membrane vesicles(OMVs)have shown great potential in cancer immunotherapy.The isolation of OMVs from complex media with high purity and high bioactivity is the prerequisite of therapeutic applications,...Bacterial outer membrane vesicles(OMVs)have shown great potential in cancer immunotherapy.The isolation of OMVs from complex media with high purity and high bioactivity is the prerequisite of therapeutic applications,which remains highly challenging.Herein,we report a smart DNA hydrogel for the efficient isolation of OMVs from bacterial culture medium,which is further applied for localized cancer immunotherapy.The DNA hydrogel is constructed through the cross-linking of two ultralong DNA chains generated via rolling circle amplification(RCA).One chain contains polyvalent GN6 aptamer for the specific capture of OMVs,and the other contains polyvalent programmed death-1(PD-1)aptamer for the blocking of PD-1 immune checkpoint on the surface of T lymphocytes.The OMVs capsulated by DNA hydrogel maintain high immunostimulatory bioactivity.In the mouse model of melanoma,this OMVs-containing DNA hydrogel shows a remarkable tumor inhibition rate of∼95%.This smart DNA hydrogel represents a promising biomedical platform for the efficient isolation of bacterial-derived OMVs,and provides a powerful strategy for cancer immunotherapy.展开更多
基金supported by the National Key Research and Development Project,China(No.2020YFA0909000)the National Natural Science Foundation of China(No.22107027)+1 种基金the Natural Science Foundation of Hunan Province,China(No.2023JJ20003)the Scientific Research Program of Furong Laboratory,China(No.2023SK2088).
文摘Membrane proteins are vital components of the cell membrane and play crucial roles in various cellular activities.Analysis of membrane proteins is of paramount importance for studying molecular events inside cells and organisms and holds promising prospects for early disease diagnosis and treatment assessment.Benefiting from obvious merits including high affinity,high specificity and ease of modification,aptamers have been regarded as ideal molecular recognition elements in membrane protein analysis and molecular diagnostics strategies.This review summarised recent advances in membrane protein-specific aptamer screening,aptamer-based static and dynamic membrane protein analysis,and aptamer-based molecular diagnostic techniques.Prospects and challenges were also discussed.
基金Supported by the National Natural Science Foundataion of China(No.21974087)the Faculty Start-up Funding Support from the Institute of Molecular Medicine of Shanghai Jiao Tong University,Chinathe Recruitment Program of Global Youth Experts of China.
文摘Recent developments in DNA nanotechnology have brought various nanoscale structures,devices and functional systems for different applications.As biological barriers with significant functions,cell membranes proide direct interfaces for studying cellular environment and states.So far,DNA nanotechnology engineered on live cell membranes has advanced our fundamental understandings of DNA nanomaterials and facilitated the designs of novel sensing,imaging and therapeutic platforms.In this review,we highlighted strategies and outcomes of using DNA nanotechnology on cell membranes towards various biomedical applications,including biosensing,imaging,cellular function regulations and targeted cancer therapy.Furthermore,we also discussed the challenges and opportunities of DNA nanotechnology on cell membranes towards broader applications.
基金the National Natural Science Foundation of China(Nos.51925304,51903175,and 51973133).
文摘The invasion of etched dentinal tubules(DTs)by external substances induces dentin hypersensitivity(DH).The deep and compact occlusion of DTs is highly desirable for treating DH but still challenging due to the limited penetrability and mineralization capacities of most current desensitizers.Matrix vesicles(MVs)participate in the regulation of ectopic mineralization.Herein,ectopic MV analogs are prepared by employing natural cell membranes to endow mineral precursors with natural biointerfaces and integrated biofunctions for stimulating dentin remineralization.The analogs quickly access DTs(>20μm)in only 5 min and further penetrate deep into the interior of DTs(an extraordinary~200μm)in 7 days.Both in vitro and in vivo studies confirm that the DTs are efficiently sealed by the newly formed minerals(>50μm)with excellent resistance to wear and acid erosion,which is significantly deeper than most reported values.After repair,the microhardness of the damaged dentin can be recovered to those of healthy dentin.For the first time,cell membrane coating nanotechnology is used as a facile and efficient therapy for in-depth remineralization of DTs in treating DH with thorough and long-term effects,which provides insights into their potential for hard tissue repair.
文摘Filtration and membrane separation are popular methods in gas separation since they are cost and energy efficient. Despite to air filters, there are comparatively few studies on dry gas filters, particularly at industrial scale. In fact, major unsolved challenges such as high efficiency, low pressure drop, long-term stability, high-thermal and chemical stability and advanced physiochemical properties, are still remained. The aim of this review is to scrutinize the advanced scientific and technological practices (such as selection of appropriate polymeric materials and additives, nanotechnology, modification techniques and preparation methods) towards design and fabrication of an efficient filter media for solid particles removal from the natural gas flow. Recent progresses in solid particle separation mechanisms, modeling and simulation techniques and the effect of membrane fabrication methods on its performance, strategies for modification of filter media, current challenges and future perspective are discussed.
基金supported by National Natural Science Foundation of China(22225505,22322407,and 22174097)Fudan University Ruiqing Education Funding。
文摘Bacterial outer membrane vesicles(OMVs)have shown great potential in cancer immunotherapy.The isolation of OMVs from complex media with high purity and high bioactivity is the prerequisite of therapeutic applications,which remains highly challenging.Herein,we report a smart DNA hydrogel for the efficient isolation of OMVs from bacterial culture medium,which is further applied for localized cancer immunotherapy.The DNA hydrogel is constructed through the cross-linking of two ultralong DNA chains generated via rolling circle amplification(RCA).One chain contains polyvalent GN6 aptamer for the specific capture of OMVs,and the other contains polyvalent programmed death-1(PD-1)aptamer for the blocking of PD-1 immune checkpoint on the surface of T lymphocytes.The OMVs capsulated by DNA hydrogel maintain high immunostimulatory bioactivity.In the mouse model of melanoma,this OMVs-containing DNA hydrogel shows a remarkable tumor inhibition rate of∼95%.This smart DNA hydrogel represents a promising biomedical platform for the efficient isolation of bacterial-derived OMVs,and provides a powerful strategy for cancer immunotherapy.
