The coal strength and deformation properties are key factors affecting safe coal mining and highefficiency coalbed methane(CBM)development.In this paper,reconstituted coal samples are chosen to investigate the weakeni...The coal strength and deformation properties are key factors affecting safe coal mining and highefficiency coalbed methane(CBM)development.In this paper,reconstituted coal samples are chosen to investigate the weakening behavior of gas on coal strength,meanwhile,its effects on coal deformation are quantitatively evaluated.The results indicate that the weakening degree of gas on coal strength is closely related to the confining stress and gas pressure.Compared with non-gas-saturated coals,the maximum weakening ratios of adsorbed gas to coal strength are 10.58%,18.12%,8.55%and 14.65%under the conditions of confining stress CS=3 MPa and gas pressure GP=1 MPa,CS=3 MPa and GP=2 MPa,CS=4 MPa and GP=1 MPa,and CS=4 MPa and GP=2 MPa,respectively.Furthermore,the maximum weakening ratios of free gas to coal strength are 18.27%,36.54%,14.79%and 29.58%,respectively,under above four conditions.The maximum coal bulk strain decreases as particle sizes of coal powders increase,and it has a maximum value of 0.0227 and a minimum value of 0.0191 in particle size ranges of 0.01–0.041 and 0.5–1 mm.Under the same conditions,the coal bulk strain increases with increasing gas pressure,revealing that coal deformation properties can be enhanced by gas.展开更多
Assessment system of implicit environmental impacts was established including environmental impact indicator, resources consumption indicator and energy consumption indicator. The quantification of environmental impac...Assessment system of implicit environmental impacts was established including environmental impact indicator, resources consumption indicator and energy consumption indicator. The quantification of environmental impact indicators is based on the life cycle assessment system of the Society of Environmental Toxicology and Chemistry (SETAC) and the evaluation software BEES. Normalization reference values and weights of 12 categories of environmental impacts were identified, and the environmental impact indicators in the phases of raw material extraction, transportation, manufacturing, use and end of life were analyzed. By analyzing the environmental performance of a university refectory as a case study, it is demonstrated that human health, global wanning and acidification are the first three environmental impacts in 12 categories. The total implicit environmental impact load per square meter of this project is 18.448 × 10 ^-2 standard human equivalent weight. Moreover, 97.3% of total environmental impacts occur in the phase of raw material extraction.展开更多
Given the lack of defining features in the clinical manifestations and radiographic findings for children with mycoplasma pneumoniae pneumonia(MPP),quantitative polymerase chain reaction(qPCR)has become a useful diagn...Given the lack of defining features in the clinical manifestations and radiographic findings for children with mycoplasma pneumoniae pneumonia(MPP),quantitative polymerase chain reaction(qPCR)has become a useful diagnostic method.This study was performed to explore the relationship between the qPCR findings,clinical symptoms,and inflammatory markers in children with MPP.Four hundred children with MPP have been enrolled in this retrospective analysis.All clinical and analytical information,including mycoplasma pneumoniae(MP)PCR results,has been collected.Based on the PCR results,the patients were divided into groups with load values(copy number)<105(54 cases),2105 and<106(71 cases),2106 and<107(112 cases),>107 and<108(114 cases),and>108(49 cases).The clinical features(including symptoms and signs)and inflammatory indicators were compared among the groups.The incidence of high fever(above 39℃),thermal peak during the entire hospitalization period,fever duration,days of hospitalization,and plasma lactate dehydrogenase(LDH)levels were statistically correlated with the MP PCR load value in children with MPP.The analysis of relevance degree showed the correlative order as a thermal peak of hospitalization>duration of fever>period of hospitalization>LDH value>C-reactive protein value.The host immune response was significantly greater in the complication group than in the non-complication group.展开更多
Plant-associated microbes are critical for plant growth and survival under natural environmental conditions.To date,most plant microbiome studies involving high-throughput amplicon sequencing have focused on the relat...Plant-associated microbes are critical for plant growth and survival under natural environmental conditions.To date,most plant microbiome studies involving high-throughput amplicon sequencing have focused on the relative abundance of microbial taxa.However,this technique does not assess the total microbial load and the abundance of individual microbes relative to the amount of host plant tissues.Here,we report the development of a host-associated quantitative abundance profiling(HA-QAP)method that can accurately examine total microbial load and colonization of individual root microbiome members relative to host plants by the copy-number ratio of microbial marker gene to plant genome.We validate the HAQAP method using mock experiments,perturbation experiments,and metagenomic sequencing.The HA-QAP method eliminates the generation of spurious outputs in the classical method based on microbial relative abundance,and reveals the load of root microbiome to host plants.Using the HA-QAP method,we found that the copy-number ratios of microbial marker genes to plant genome range from 1.07 to 6.61 for bacterial 16S rRNA genes and from 0.40 to 2.26 for fungal internal transcribed spacers in the root microbiome samples from healthy rice and wheat.Furthermore,using HA-QAP we found that an increase in total microbial load represents a key feature of changes in root microbiome of rice plants exposed to drought stress and of wheat plants with root rot disease,which significantly influences patterns of differential taxa and species interaction networks.Given its accuracy and technical feasibility,HA-QAP would facilitate our understanding of genuine interactions between root microbiome and plants.展开更多
目的:利用T-Scan定量分析单颗后牙种植修复体在牙尖交错位(intercuspal position,ICP)时咬合接触情况,并探讨其对种植体周围垂直骨吸收的影响。方法:选取第一磨牙常规种植修复的40例无咬合高点等不良修复现象和磨牙症等不良习惯的患者。...目的:利用T-Scan定量分析单颗后牙种植修复体在牙尖交错位(intercuspal position,ICP)时咬合接触情况,并探讨其对种植体周围垂直骨吸收的影响。方法:选取第一磨牙常规种植修复的40例无咬合高点等不良修复现象和磨牙症等不良习惯的患者。T-Scan记录种植修复体及对侧同名牙咬合接触情况的差异,分别按照咬合延迟时间、牙合力百分率比、咬合力峰值及咬合接触面积比进行分组,用影像学测量技术评估种植体周围骨组织的变化,从而分析咬合因素对种植体周围垂直骨吸收(vertical bone loss,VBL)的影响,并建立多元线性回归模型。结果:在咬合延迟时间和咬合力峰值分组比较中,平均VBL均存在显著性差异(P<0.05)。同时得出预测方程:Y=1.060-0.734X1+0.248X2-0.589X3,其中Y为平均VBL、X1为咬合延迟时间、X2为咬合力峰值、X3为咬合接触面积比。结论:在单颗后牙缺失常规种植修复时除考虑适当的咬合设计外,在调整种植修复体的咬合接触或定期咬合检查时,推荐应用T-Scan进行定量分析以确保ICP时咬合力峰值尽可能降低(即轻咬合接触),和适当延迟种植修复体首次咬合接触时间,对维持种植体周围骨的稳定性极为有益。展开更多
基金financial support from the National Natural Science Foundation of China(Nos.51904310and 51874314)the Fundamental Research Funds for the Central Universities(No.2020YQAQ03)+2 种基金the Open Funds of Key Laboratory of Safety and High-efficiency Coal MiningMinistry of Education(Anhui University of Science and Technology)(No.JYBSYS2019204)the Open Funds of State Key Laboratory Cultivation Base for Gas Geology and Gas Control(Henan Polytechnic University)(No.WS2018B06)。
文摘The coal strength and deformation properties are key factors affecting safe coal mining and highefficiency coalbed methane(CBM)development.In this paper,reconstituted coal samples are chosen to investigate the weakening behavior of gas on coal strength,meanwhile,its effects on coal deformation are quantitatively evaluated.The results indicate that the weakening degree of gas on coal strength is closely related to the confining stress and gas pressure.Compared with non-gas-saturated coals,the maximum weakening ratios of adsorbed gas to coal strength are 10.58%,18.12%,8.55%and 14.65%under the conditions of confining stress CS=3 MPa and gas pressure GP=1 MPa,CS=3 MPa and GP=2 MPa,CS=4 MPa and GP=1 MPa,and CS=4 MPa and GP=2 MPa,respectively.Furthermore,the maximum weakening ratios of free gas to coal strength are 18.27%,36.54%,14.79%and 29.58%,respectively,under above four conditions.The maximum coal bulk strain decreases as particle sizes of coal powders increase,and it has a maximum value of 0.0227 and a minimum value of 0.0191 in particle size ranges of 0.01–0.041 and 0.5–1 mm.Under the same conditions,the coal bulk strain increases with increasing gas pressure,revealing that coal deformation properties can be enhanced by gas.
基金The Research Fund for the Doctoral Pro-gram of Higher Education of China(No.20050487017)
文摘Assessment system of implicit environmental impacts was established including environmental impact indicator, resources consumption indicator and energy consumption indicator. The quantification of environmental impact indicators is based on the life cycle assessment system of the Society of Environmental Toxicology and Chemistry (SETAC) and the evaluation software BEES. Normalization reference values and weights of 12 categories of environmental impacts were identified, and the environmental impact indicators in the phases of raw material extraction, transportation, manufacturing, use and end of life were analyzed. By analyzing the environmental performance of a university refectory as a case study, it is demonstrated that human health, global wanning and acidification are the first three environmental impacts in 12 categories. The total implicit environmental impact load per square meter of this project is 18.448 × 10 ^-2 standard human equivalent weight. Moreover, 97.3% of total environmental impacts occur in the phase of raw material extraction.
基金This study was supported by the Chongqing Science and Health Joint Medical Research Project(No.8187011078)。
文摘Given the lack of defining features in the clinical manifestations and radiographic findings for children with mycoplasma pneumoniae pneumonia(MPP),quantitative polymerase chain reaction(qPCR)has become a useful diagnostic method.This study was performed to explore the relationship between the qPCR findings,clinical symptoms,and inflammatory markers in children with MPP.Four hundred children with MPP have been enrolled in this retrospective analysis.All clinical and analytical information,including mycoplasma pneumoniae(MP)PCR results,has been collected.Based on the PCR results,the patients were divided into groups with load values(copy number)<105(54 cases),2105 and<106(71 cases),2106 and<107(112 cases),>107 and<108(114 cases),and>108(49 cases).The clinical features(including symptoms and signs)and inflammatory indicators were compared among the groups.The incidence of high fever(above 39℃),thermal peak during the entire hospitalization period,fever duration,days of hospitalization,and plasma lactate dehydrogenase(LDH)levels were statistically correlated with the MP PCR load value in children with MPP.The analysis of relevance degree showed the correlative order as a thermal peak of hospitalization>duration of fever>period of hospitalization>LDH value>C-reactive protein value.The host immune response was significantly greater in the complication group than in the non-complication group.
基金This work is financially supported by the National Natural Science Foundation of China(grant nos.31772400,31761143017)the National Natural Science Foundation for Young Scientists of China(grant no.31701997)+1 种基金the Key Research Program of the Chinese Academy of Sciences(grant nos.KFZD-SW-112-02-02 and KFZD-SW-219)the Key Research Program of Frontier Sciences,CAS(grant no.QYZDB-SSW-SMC021).
文摘Plant-associated microbes are critical for plant growth and survival under natural environmental conditions.To date,most plant microbiome studies involving high-throughput amplicon sequencing have focused on the relative abundance of microbial taxa.However,this technique does not assess the total microbial load and the abundance of individual microbes relative to the amount of host plant tissues.Here,we report the development of a host-associated quantitative abundance profiling(HA-QAP)method that can accurately examine total microbial load and colonization of individual root microbiome members relative to host plants by the copy-number ratio of microbial marker gene to plant genome.We validate the HAQAP method using mock experiments,perturbation experiments,and metagenomic sequencing.The HA-QAP method eliminates the generation of spurious outputs in the classical method based on microbial relative abundance,and reveals the load of root microbiome to host plants.Using the HA-QAP method,we found that the copy-number ratios of microbial marker genes to plant genome range from 1.07 to 6.61 for bacterial 16S rRNA genes and from 0.40 to 2.26 for fungal internal transcribed spacers in the root microbiome samples from healthy rice and wheat.Furthermore,using HA-QAP we found that an increase in total microbial load represents a key feature of changes in root microbiome of rice plants exposed to drought stress and of wheat plants with root rot disease,which significantly influences patterns of differential taxa and species interaction networks.Given its accuracy and technical feasibility,HA-QAP would facilitate our understanding of genuine interactions between root microbiome and plants.
文摘目的:利用T-Scan定量分析单颗后牙种植修复体在牙尖交错位(intercuspal position,ICP)时咬合接触情况,并探讨其对种植体周围垂直骨吸收的影响。方法:选取第一磨牙常规种植修复的40例无咬合高点等不良修复现象和磨牙症等不良习惯的患者。T-Scan记录种植修复体及对侧同名牙咬合接触情况的差异,分别按照咬合延迟时间、牙合力百分率比、咬合力峰值及咬合接触面积比进行分组,用影像学测量技术评估种植体周围骨组织的变化,从而分析咬合因素对种植体周围垂直骨吸收(vertical bone loss,VBL)的影响,并建立多元线性回归模型。结果:在咬合延迟时间和咬合力峰值分组比较中,平均VBL均存在显著性差异(P<0.05)。同时得出预测方程:Y=1.060-0.734X1+0.248X2-0.589X3,其中Y为平均VBL、X1为咬合延迟时间、X2为咬合力峰值、X3为咬合接触面积比。结论:在单颗后牙缺失常规种植修复时除考虑适当的咬合设计外,在调整种植修复体的咬合接触或定期咬合检查时,推荐应用T-Scan进行定量分析以确保ICP时咬合力峰值尽可能降低(即轻咬合接触),和适当延迟种植修复体首次咬合接触时间,对维持种植体周围骨的稳定性极为有益。