3维荧光光谱测定白酒年份酒中乙酸的体积分数

为了快速测定白酒年份酒中乙酸的体积分数,采用3维荧光光谱结合交替拟合残差算法,首先将不同体积分数的乙酸乙醇水溶液的3维荧光光谱作为校正集,然后将白酒的3维荧光光谱作为预测集,利用交替拟合残差算法进行解析分辨,采用标准添加法来验证结果的准确性。结果表明,预测体积分数与真实体积分数的相关系数为0.9926,平均回收率为101.97%;3维荧光光谱结合交替拟合残差算法可以快速有效地测定白酒年份酒中乙酸的体积分数。这一结果对白酒年份酒中单体体积分数的检测是有帮助的。

激发-发射荧光矩阵结合二阶校正方法检测湖水中多环芳烃

多环芳烃广泛存在于大气、土壤和水环境中,对动植物和人类有着严重危害。为了快速检测水环境中的痕量多环芳烃(Polycyclic Aromatic Hydrocarbons,PAHs),本文提出利用激发-发射荧光矩阵结合自加权交替归一残差拟合算法(Self-Weighted Alternating Normalized Residual Fitting Algorithm,SWANRF)检测湖水中的菲、蒽和荧蒽。与自加权交替三线性分解方法相比,SWANRF能够给出更满意的浓度预测结果,菲、蒽和荧蒽的平均回收率分别为(99.2±7.2)%,(101.7±7.7)%和(97.9±5.1)%;菲、蒽和荧蒽的预测均方根误差值分别为0.240,0.249和0.247μg/L。实验结果表明,文章提出的方法能够实现未知干扰物共存的湖水中痕量多环芳烃的快速检测,且方法可靠。

Second-order calibration applied to quantification of two active components of Schisandra chinensis in complex matrix

The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use. The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry. The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices. Although the spectra of the analytes are similar and a large number of interferences also exist, second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its ‘second-order advantage’. Moreover, the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents.