Objective: To explore a new way for developing the human umbilical cord blood (CB) engraftment with high efficiency and in further understand the homing potentiality of hematopoietic stein/progenitor cells (HSC/HPC). ...Objective: To explore a new way for developing the human umbilical cord blood (CB) engraftment with high efficiency and in further understand the homing potentiality of hematopoietic stein/progenitor cells (HSC/HPC). Method: Sub-lethally irradiated severe combined immunodeficient (SCID) mice were transplanted i. v. with CB which was cryopre- served at - 80℃. Human cells in recipient mice were detected by flow cytometry and CFU-GM assay for each host organ. Results: In contrast with the single transplantation, scheduled engraftment of the identical numbers of CB cells resulted in an obvious increase of CD45+ and CD34+ cells produced in SCID mouse bone marrow (BM). While the donor cells were reduced by 5 times, the similar reconstitution of both hematopoietic fumctions and part of immunologic fumctions was ob- tained.Conclusion:Scheduled engraftment can improve repopulating of the HSC, which provides a novel way for clinical cord blood engraftment into adults.展开更多
基金upported by National Natural Science Foundation of China, No. 39770329
文摘Objective: To explore a new way for developing the human umbilical cord blood (CB) engraftment with high efficiency and in further understand the homing potentiality of hematopoietic stein/progenitor cells (HSC/HPC). Method: Sub-lethally irradiated severe combined immunodeficient (SCID) mice were transplanted i. v. with CB which was cryopre- served at - 80℃. Human cells in recipient mice were detected by flow cytometry and CFU-GM assay for each host organ. Results: In contrast with the single transplantation, scheduled engraftment of the identical numbers of CB cells resulted in an obvious increase of CD45+ and CD34+ cells produced in SCID mouse bone marrow (BM). While the donor cells were reduced by 5 times, the similar reconstitution of both hematopoietic fumctions and part of immunologic fumctions was ob- tained.Conclusion:Scheduled engraftment can improve repopulating of the HSC, which provides a novel way for clinical cord blood engraftment into adults.
