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Separation of non-denatured proteins using semi-crosslinked polyacrylamide capillary gel electrophoresis
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作者 XU Jian-dong HU Ding-yu +2 位作者 BAKTUR Rena GENG Li-na DENG Yu-lin 《Journal of Beijing Institute of Technology》 EI CAS 2014年第1期131-137,共7页
This work presents an approach to build a high-performance, low-viscous and replaceable separation matrix, semi-crosslinked polyacrylamide (semi-CPA) capillary gel electrophoresis. Non- denatured basic proteins, suc... This work presents an approach to build a high-performance, low-viscous and replaceable separation matrix, semi-crosslinked polyacrylamide (semi-CPA) capillary gel electrophoresis. Non- denatured basic proteins, such as lysozyme, cytochrome C, ribonuclease A and trypsin were separa- ted. The impacts of monomer and cross-linker concentrations on protein separation were studied, and the ability of dynamic capillary inner wall coating was demonstrated. The UV absorption interfer- ence by semi-CPA gel matrix was successfully overcome by a partial filling technique, which results in sensitivity 20 times higher than other protein separation method. The excellent separation ability, reproducibility and dynamic coating ability made semi-CPA an ideal separation media in both capillar- y electrophoresis and microfluidic chip separation scheme. 展开更多
关键词 capillary gel electrophoresis semi-crosslinked polyacrylamide (semi-CPA) non-dena-tured protein separation
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