Estimation-free spatial-domain image reconstruction of structured illumination microscopy

Structured illumination microscopy(SIM)achieves super-resolution(SR)by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction.The traditional Wiener-filtering-based reconstruction algorithm operates in the Fourier domain,it requires prior knowledge of the sinusoidal illumination patterns which makes the time-consuming procedure of parameter estimation to raw datasets necessary,besides,the parameter estimation is sensitive to noise or aberration-induced pattern distortion which leads to reconstruction artifacts.Here,we propose a spatial-domain image reconstruction method that does not require parameter estimation but calculates patterns from raw datasets,and a reconstructed image can be obtained just by calculating the spatial covariance of differential calculated patterns and differential filtered datasets(the notch filtering operation is performed to the raw datasets for attenuating and compensating the optical transfer function(OTF)).Experiments on reconstructing raw datasets including nonbiological,biological,and simulated samples demonstrate that our method has SR capability,high reconstruction speed,and high robustness to aberration and noise.

Efficacy and safety of Usights UC100 illuminated microcatheter in microcatheter-assisted trabeculotomy

AIM:To evaluate the efficacy and safety of Usights UC100 illuminated microcatheter in microcatheter-assisted trabeculotomy(MAT).METHODS:Totally 10 eyes of 10 patients with primary open angle glaucoma(POAG)who underwent MAT facilitated by Usights UC100(5 eyes)or iTrack(5 eyes)were reviewed.The success of this surgery was defined as intraocular pressure(IOP)<22 mm Hg with>30%reduction,without oral glaucoma medications,or additional glaucoma surgery.RESULTS:The mean pre-operative IOP was 25.38±10.22 mm Hg in the Usights UC100 group and 19.98±3.87 mm Hg in the iTrack group.MAT was achieved in all eyes in both groups.The success rates for the Usights UC100 group and iTrack groups were in all and 4 eyes,respectively.Both microcatheters produced a statistically significant reduction in IOP,and eyes using Usights UC100 achieved a lower IOP than the iTrack group at 3mo followup(12.58±1.52 and 14.84±1.89 mm Hg,respectively),but no statistical significance was there.No severe side effects were observed in either group.CONCLUSION:MAT using Usights UC100 or iTrack both achieve significant pressure reduction in cases of POAG,and Usights UC100 is as safe as iTrack.

Numerical Simulation of Super-Resolution Structured Illumination Microscopy (SIM) Using Heintzmann-Cremer Algorithm with Non-Continuous Spatial Frequency Support

We report a comprehensive numerical study of super resolution (SR) structured illumination microscopy (SIM) utilizing the classic Heintzmann-Cremer SIM process and algorithm. In particular, we investigated the impact of the diffraction limit of the underlying imaging system on the optimal SIM grating frequency that can be used to obtain the highest SR enhancement with non-continuous spatial frequency support. Besides confirming the previous theoretical and experimental work that SR-SIM can achieve an enhancement close to 3 times the diffraction limit with grating pattern illuminations, we also observe and report a series of more subtle effects of SR-SIM with non-continuous spatial frequency support. Our simulations show that when the SIM grating frequency exceeds twice that of the diffraction limit, the higher SIM grating frequency can help achieve a higher SR enhancement for the underlying imaging systems whose diffraction limit is low, though this enhancement is obtained at the cost of losing resolution at some lower resolution targets. Our simulations also show that, for underlying imaging systems with high diffraction limits, however, SR-SIM grating frequencies above twice the diffraction limits tend to bring no significant extra enhancement. Furthermore, we observed that there exists a limit grating frequency above which the SR enhancement effect is lost, and the reconstructed images essentially have the same resolution as the one obtained directly from the underlying imaging system without using the SIM process.

Antiplane response of two scalene triangular hills and a semi-cylindrical canyon by incident SH-waves

Antiplane response of two scalene triangular hills and a semi-cylindrical canyon by SH-waves is studied using wave function expansion and complex function method. Firstly, the analytical model is divided into three parts, and the displacement solutions of wave fields are constructed based on boundary conditions in the three regions. Three domains are then conjoined to satisfy the ＂conjunction＂ condition at shared boundary. In addition, combined with the zero-stress condition of semi-cylindrical canyon, a series of infinite algebraic equations for the problem are derived. Finally, numerical examples are provided and the influence of different parameters on ground motion is discussed.

Speckle structured illumination endoscopy with enhanced resolution at wide field of view and depth of field

Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically limited to two times of the diffraction limit and the depth of field is small.In this work,we propose and experimentally demonstrate a low cost,easy to implement,novel technique called speckle structured illumination endoscopy(SSIE)to enhance the resolution of a wide field endoscope with large depth of field.Here,speckle patterns are used to excite objects on the sample which is then followed by a blind-SIM algorithm for super resolution image reconstruction.Our approach is insensitive to the 3D morphology of the specimen,or the deformation of illuminations used.It greatly simplifies the experimental setup as there are no calibration protocols and no stringent control of illumination patterns nor focusing optics.We demonstrate that the SSIE can enhance the resolution 2–4.5 times that of a standard white light endoscopic(WLE)system.The SSIE presents a unique route to super resolution in endoscopic imaging at wide field of view and depth of field,which might be beneficial to the practice of clinical endoscopy.

Reflector Design Method for Rotational Uniform Illuminance System with LED

Based on nonimaging design method, uniform illuminance systems with LED source were developed to create a uniform illuminated circular region with a desired size in a screen at a prescribed place. By using ray-tracing software based on Monte-Carlo method, the simulation results show that in the illuminated region the luminous uniformity is higher than 90%.

Scattering of SH-waves on triangular hill joined bysemi-cylindrical canyon

Scattering of SH-waves on the triangular hill joined by semi-cylindrical canyon in half-space is studied using the method of complex function and moving coordinates. The model being studied is divided into two domains. The wave functions satisfying the required condition at each wedge are constructed in each equation. The equations are solved with Fourier expansion. Numerical results are provided to discuss the influence of scattering of SH-waves.

Complex-amplitude Fourier single-pixel imaging via coherent structured illumination

We propose a method of complex-amplitude Fourier single-pixel imaging(CFSI)with coherent structured illumination to acquire both the amplitude and phase of an object.In the proposed method,an object is illustrated by a series of coherent structured light fields,which are generated by a phase-only spatial light modulator,the complex Fourier spectrum of the object can be acquired sequentially by a single-pixel photodetector.Then the desired complex-amplitude image can be retrieved directly by applying an inverse Fourier transform.We experimentally implemented this CFSI with several different types of objects.The experimental results show that the proposed method provides a promising complex-amplitude imaging approach with high quality and a stable configuration.Thus,it might find broad applications in optical metrology and biomedical science.

Distributed Intelligent Lighting System by Performing New Model for Illuminance and Color Temperature in the Workplace

A new approach has been proposed to improve the performance of the in-telligent lighting system by estimating personal illuminance and desired color temperature at the workplace. We are considering the problem of using the sensing devices manually for the intelligent lighting system. The lighting control system has not become useful without sensing devices to measure the provided illuminance and color temperature. In this paper, we have used the property of light for the color temperature to estimate the level of color temperature for each user at the workplace. The new method will give personal illuminance for each user at the workplace and decrease the power consumption of the environment as well. As a result, the proposed method of the intelligent lighting system has realized the target of illuminance and color temperature for each user at the workplace by adapting dimming levels using illuminance sensing information for each user. Thus, the energy of the workplace has reduced by using a distributed luminance to realize the target for each user.

Comparison of point detection and area detection for point-scanning structured illumination microscopy

Structured illumination microscopy(SIM)is suitable for biological samples because of its relatively low-peak illumination intensity requirement and high imaging speed.The system resolution is affected by two typical detection modes:Point detection and area detection.However,a systematic analysis of the imaging performance of the different detection modes of the system has rarely been conducted.In this study,we compared laser point scanning point detection(PS-PD)and point scanning area detection(PS-AD)imaging in nonconfocal microscopy through theoretical analysis and simulated imaging.The results revealed that the imaging resolutions of PSPD and PS-AD depend on excitation and emission point spread functions(PSFs),respectively.Especially,we combined the second harmonic generation(SHG)of point detection(P-SHG)and area detection(A-SHG)with SIM to realize a nonlinear SIM-imaging technique that improves the imaging resolution.Moreover,we analytically and experimentally compared the nonlinear SIM performance of P-SHG with that of A-SHG.

Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging

The miniaturized femtosecond laser in near infrared-Ⅱregion is the core equipment of threephoton microscopy.In this paper,we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy.Based on an all-polarizationmaintaining passive mode-locked fiber laser,we shift the center wavelength of the pulses to the 1.7m band utilizing cascade Raman effect,thereby generate dual-wavelength pulses.To enhance clarity,the two wavelengths are separated through the graded-index multimode fiber.Then we obtain the dual-pulse sequences with 1639.4 nm and 1683.7 nm wavelengths,920 fs pulse duration,and 23.75 MHz pulse repetition rate.The average power of the signal is 53.64mW,corresponding to a single pulse energy of 2.25 nJ.This illumination source can be further amplified and compressed for three-photon fluorescence imaging,especially dual-color three-photon fluorescence imaging,making it an ideal option for biomedical applications.

Comparison of Illuminance in Different Environments: A Case Study on Subway Stations

With urbanization and the rapid development of social economy,China’s rail transit industry has developed rapidly in recent years.In order to alleviate the pressure of road network,subways provide convenience as they are fast and space-saving.Subway stations are major energy consumers of urban power grid due to their large traffic volume and long operation time.On the premise of ensuring operation safety,reducing the energy consumption of subway helps in energy conservation and emission reduction as proposed in the 13th Five-Year Plan.According to the statistics of the energy-saving evaluation report of rail transit engineering,the lighting system accounts for 20%-30%of the total power consumption of the subway station.Due to the single lighting control mode of the lighting system in the subway station,the actual station illumination cannot be reported and adjusted in time,resulting in the waste of lighting energy and high power consumption of the system.Through in-depth research on the intelligent lighting system of subway station,this paper improves the system control,and finally summarizes the optimization scheme of subway station lighting design which can effectively save the power consumption of lighting system.The main contents of this paper are as follows:The research results of this paper can provide effective measures for energy saving of electric lighting in subway stations and reduce electric energy consumption;on the other hand,as an integral part of building lighting energy-saving system,it also has certain guiding significance for the research of building lighting energy-saving.

Radial Basis Approximations Based BEMD for Enhancement of Non-Uniform Illumination Images

An image can be degraded due to many environmental factors like foggy or hazy weather,low light conditions,extra light conditions etc.Image captured under the poor light conditions is generally known as non-uniform illumination image.Non-uniform illumination hides some important information present in an image during the image capture Also,it degrades the visual quality of image which generates the need for enhancement of such images.Various techniques have been present in literature for the enhancement of such type of images.In this paper,a novel architecture has been proposed for enhancement of poor illumination images which uses radial basis approximations based BEMD(Bi-dimensional Empirical Mode Decomposition).The enhancement algorithm is applied on intensity and saturation components of image.Firstly,intensity component has been decomposed into various bi-dimensional intrinsic mode function and residue by using sifting algorithm.Secondly,some linear transformations techniques have been applied on various bidimensional intrinsic modes obtained and residue and further on joining the transformed modes with residue,enhanced intensity component is obtained.Saturation part of an image is then enhanced in accordance to the enhanced intensity component.Final enhanced image can be obtained by joining the hue,enhanced intensity and enhanced saturation parts of the given image.The proposed algorithm will not only give the visual pleasant image but maintains the naturalness of image also.

我国24家检验检测机构洁净环境照度检测能力验证

目的评价我国检验检测机构对洁净环境照度的检测能力。方法回顾性分析中国食品药品检定研究院于2022年在全国范围内首次设计并组织实施的洁净环境照度能力验证项目。基于洁净环境检测的行业特点,该项目分为笔试和实际操作(简称实操)两部分。笔试部分,共3题(限时40 min完成),考察参加测试(简称参测)实验室对检测标准正确理解和掌握的程度,根据专家商议确定的评分标准评分(满分50分)。实操部分,以该院药用辅料和包装材料检定所的2台洁净工作台为现场考核样品,以参测实验室的现场测试结果考察参测实验室的仪器操作和实验水平(限时5 min完成),以Z值进行评价(︱Z︱≤2,>2~<3,≥3分别为满意、有问题、不满意)并打分(满分50分)。以笔试和实操评分之和评价参测实验室的检测能力(≥60分为合格)。结果共24家实验室参加了能力验证并按要求提交了结果报告。笔试,评分为45~50分,满分21家(87.50%)。实操,样品A,满意21家(87.50%),有问题3家(12.50%);样品B,满意23家(95.83%),有问题1家(4.17%),2个样品均无不满意的实验室。24家实验室总评分均合格。结论该次能力验证结果显示,各实验室的检测能力维持良好,但部分实验室存在对标准的理解和掌握仍有欠缺或未按校准周期对照度计进行计量等问题,建议检测人员加深对洁净环境检测标准的理解,相应机构重视仪器的校准,持续提高检测能力。

长三角地区集中式露地农业光伏方阵夏季光热环境试验研究

基于太阳照度和环境温度逐时变化,以长三角地区的集中式露地农业光伏方阵为研究对象,在跨度为6.8 m的单跨方阵中沿从南到北的跨度方向取0.65、3.40、6.15 m处的3个和长度方向共面的测试断面,即南区、中区、北区。基于此,进行夏季方阵内外的光热环境测试,分析并明确光伏组件下方区域的光热环境变化规律,为其太阳能资源合理利用、结构改进和夏季农业生产管理提供参考。结果表明,该地区光伏方阵夏季内部3个区域光热环境呈现规律性差异,整体表现为北区略优于南区,中区优于南、北两区。综合考虑作物生长的光热环境需求,一方面可通过结构改进、种植方式优化等方式改善或利用好内部光环境;另一方面基于热环境规律,分类做好内部3个区的夏季灌溉等田间管理,从而推动光伏农业发展升级。

基于LMIENet图像增强的矿井下低光环境目标检测方法

煤矿井下工作环境复杂,存在人造光源亮度低、粉尘多和水气密度大等不利因素,导致现有的目标检测算法在应用到煤矿井下时,存在提取特征困难、目标识别和定位精度低等问题。提出一种煤矿井下低照度环境目标检测算法,由矿井低光图像增强模块LMIENet和目标检测模块组成,使用图像增强模块对原始图像进行画质提升,恢复各类图像信息,再使用目标检测网络对增强图像进行特定目标检测,有效提高检测的精确度。在图像增强模块中,改进Zero-DCE算法设计轻量级增强参数预测网络,计算像素级增强参数矩阵,用于低光照图像的亮度调整和画质增强,该网络通过设计的非参考损失函数隐性衡量图像的增强效果,引导网络进行无监督学习,使网络能够不依赖配对数据集对原始图像进行自适应的画质增强。目标检测模块中,采用YOLO v8n目标检测模型,其轻量化的模型尺寸和高灵活性可避免模型整体复杂度过高;采用Focal-EIoU Loss改进回归损失函数,有效加速模型收敛并提升模型检测精度。实验结果显示:与经典目标检测算法Faster R–CNN,SSD,RetinaNet,FCOS等相比,提出算法在自建矿井人员数据集上表现出色,低光照环境下目标检测的mAP@0.5达到98.0%,mAP@0.5∶0.95达64.8%,在实验环境中单帧图像推理时间仅11 ms,优于其他对比方法,证明提出算法能够有效实现在煤矿井下低照度复杂环境下的目标检测,且耗时短、计算效率高。

一种基于暗亮通道分割融合的低照度环境图像去尘雾及增强方法

受煤矿井下粉尘、水雾和低照度环境影响,对皮带运输系统的监测图像精准识别极为困难。针对现有去尘雾方法的图像处理结果和效率欠佳的问题,提出一种基于暗亮通道分割融合的低照度环境图像去尘雾及增强方法。首先利用阈值分割结合伽马变换修正通道差,解决因低照度环境影响导致的尘雾浓度较大区域与其他区域间像素值差异不明显的问题,修正后通过引导尘雾图像做引导滤波得到更加符合实际情况的全局大气光强;然后为解决暗通道先验在尘雾浓度较大区域失效问题,引入亮通道先验进行补充,使用通道分量来辅助暗通道及亮通道透射率融合,避免因多次分割而导致的边缘像素归属问题;最后将去雾后RGB图像转至HSV空间,对亮度分量进行直方图均衡化并将均衡化前后的亮度分量进行加权融合,采用客观指标评价,选择最优聚合权值进行聚合,同时考虑去雾过程中饱和度损失和亮度分量与饱和度分量间的相关性提出饱和度自适应矫正函数,对图像饱和度进行矫正,色调分量保持不变,随后将图像转回至RGB空间,得到亮度适中、信息保留丰富和色彩鲜艳的图像;为验证所提方法的有效性,采用主观视觉、客观指标和目标检测精度及置信度进行算法对比,实验结果表明所提方法在上述4个指标上均优于被对比算法,其图像细节保留丰富,图像视觉观感更佳。

基于超视距的外辐射源信号提取在被动探测中的应用分析

目前外辐射源雷达主要以接收辐射源的直达波信号为主,该方式提取的直达波信号信噪比高,利于后续目标信号检测和处理,但需要收发站通视,对直达波天线架高有要求,这极大地限制了该体制的应用。基于此,文中提出在沿海低海拔条件下,利用超视距侦察截获辐射源信号成为非合作探测的基准信号的思路,分析了其探测机理,和理论误差模型,并结合工程实际,提出了可实现的工程样机,分析了性能指标,给出了仿真结果。结果表明该思路可以应用在后续的工程实践上。

基于CLAHE-PCA的矿井低照度图像增强研究

地下矿山巷道环境往往面临光线不足,难以通过获取其暗通道图像判断岩体剥落等异常情况。针对矿井巷道暗通道图像对比度低的问题,提出了一种基于CLAHE-PCA的图像增强算法。首先使用CLAHE算法将获取的矿井巷道原始暗通道图像做对比度增强处理,然后使用自适应Gamma算法对亮度低的图像予以增加对比度矫正;将矫正后获得的灰度图转为RGB图像,通过PCA对其进行平滑处理,以便更多地还原暗通道图像的细节。以峰值信噪比、结构相似性、平均梯度和信息熵等作为评价指标,对试验结果进行验证。结果表明:该方法能够有效处理低对比度的矿井巷道图像,处理后的图像结构相似性达到93%,鲁棒性强,同时能够更多地还原图像的细节。

一种低照度场景下的视觉定位技术

针对在低照度环境中由于光照不足或光照不均导致的图像噪声过大、特征提取不均匀等问题,提出了一种低照度场景下的单目视觉定位技术。首先,利用微光传感器采集低照度图像信息,对图像噪声设计了一种基于深度学习的图像去噪网络,利用该网络进行图像噪声处理。然后,利用四叉树改进特征均匀提取策略以提高特征跟踪效果,采用对极几何、三角测量等技术估计图像帧间位姿。最后,构建视觉重投影误差方程,利用光束平差法进行位姿估计和优化。实验结果表明,所提定位技术在光照强度为0.01 lx的低照度环境中,轨迹闭环情况下的平均定位均方根误差小于1.47 m,轨迹无闭环情况下的平均定位均方根误差小于4.26 m。