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Dynamic change of xylanase activity and gene expression during wheat germination on As(III) stress
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作者 LI Chun-xi LI Dan-dan SHAO Yun FENG Shu-li ZHANG Dai-jing ZHANG Bei-bei JIANG Li-na 《Journal of Life Sciences》 2008年第5期45-50,共6页
Through water cultivating method, the dynamic changes of xylanase activity in seed, root and plumule of wheat with different As (III) concentration treatment were studied. The results indicated that the order of ave... Through water cultivating method, the dynamic changes of xylanase activity in seed, root and plumule of wheat with different As (III) concentration treatment were studied. The results indicated that the order of average xylanase activity was seed〉plumule〉root. With the increasing concentration of As (III), the xylanase activity elevated first then dropped in seed, but it descended first then ascended in root and plumule. As the sampling time prolonged, the xylanase activity of seeds climbed first then dropped on the four as (III) concentration, the same trend also appeared in pulume, as the as (Ill) concentration went up, the xylanase activity moved up simultaneity. Semi-quantity Reverse Transcription Polymerase Chain Reaction was used in the study, the results indicated that, the xylanase gene began to express at 132 h on 0 mg/L As (III) concentration and at 120h on other concentration in the leaves of wheat. 展开更多
关键词 As(III) stress wheat xylanase xylanase gene actingene semi-quantity reverse transcription polymerase chainreaction
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