Diagnosis related group grouping study of senile cataract patients based on E-CHAID algorithm

AIM： To figure out the contributed factors of the hospitalization expenses of senile cataract patients（HECP） and build up an area-specified senile cataract diagnosis related group（DRG） of Shanghai thereby formulating the reference range of HECP and providing scientific basis for the fair use and supervision of the health care insurance fund.METHODS： The data was collected from the first page of the medical records of 22 097 hospitalized patients from tertiary hospitals in Shanghai from 2010 to 2012 whose major diagnosis were senile cataract. Firstly, we analyzed the influence factors of HECP using univariate and multivariate analysis. DRG grouping was conducted according to the exhaustive Chi-squared automatic interaction detector（E-CHAID） model, using HECP as target variable. Finally we evaluated the grouping results using non-parametric test such as Kruskal-Wallis H test, RIV, CV, etc.RESULTS： The 6 DRGs were established as well as criterion of HECP, using age, sex, type of surgery and whether complications/comorbidities occurred as the key variables of classification node of senile cataract cases.CONCLUSION： The grouping of senile cataract cases based on E-CHAID algorithm is reasonable. And the criterion of HECP based on DRG can provide a feasible way of management in the fair use and supervision of medical insurance fund.

The Study of G6PD in Erythrocyte and Lens in Senile and Presenile Cataract

The G6PD activity of erythrocytes in 113 male patients with senile and presenile cataract and 86 controls, and G6PD activity of lens in 30 patients with senile cataract and 42 controls were reported. The cataractous group had higher frequency of G6PD deficiency and lower average G6PD level in erythrocytes and lenses, but with out statistical significance. The frequency of G6PD deficiency of erythrocytes in presenile cataractous group was higher than that of senile cataractous group but with no statistic...

Mitochondrial proteomic analysis of ecdysterone protection against oxidative damage in human lens epithelial cells

AIM: To investigate the protective effects of the natural medicinal monomer ecdysterone(ECR) with estrogenic activity against oxidative damage in human lens epithelial cells B3(HLE-B3) caused by hydrogen peroxide 21(H2 O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects. · METHODS: HLE-B3 cells were treated with H2O2(300μmol/L),β-estuarial(E2; 10-8mol/L) and H2 O2,ECR(10-6mol/L) and H2 O2,or left untreated. Altered expression of all mitochondrial proteins was analyzed by protein array and surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS). The mass/charge(M/Z) ratios of each peak were tested by the Kruskal-Wallis rank sum test,and the protein peak value of the M/Z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. ·RESULTS: H2O2 up-regulated expression of two protein spots(with M/Z of 6 532 and 6 809). When E2 mitigated the oxidative damage,the expression of one protein spot(M/Z 6 532) was down-regulated. In contrast,ECR downregulated both of protein spots(M/Z 6 532 and 6 809). · CONCLUSION: ECR could effectively inhibite H2O2 induced oxidative damage in HLE-B3 cells. The protein spot at M/Z of 6 532 might be the target spot of ECR against oxidative damage induced by H2 O2.

Clinical Study on Early Senile Cataract Treated by Shezhu Mingmu Powder(麝珠明目散)

Objective: To evaluate the clinical effect of Shezhu Mingmu Powder (SZMMP) in treatment of early senile cataract. Methods: A comparison was made between the treatment group, 162 eyes of 92 cases of early senile cataract treated by SZMMP, and the control group, 57 eyes of 30 cases treated by drops baineitini.The treatment periods were one to three months. Results: The total effective rate for the treatment group was79. 01 % and the rate of obvious improvement was 25. 31 %. In the treatment group both rateswere clearly higher than the rates (36. 84 % and 7. 02 % ) of the control group. After 4 to 8 months' following up, treatment of 42 cases showed that most of them reached the best curative effect within 1 to 3 months. Conclusion: SZMMP is obviously effective in improving visual acuity of early senile cataract, but the lens opacity was not changed.

Mitochondrial Proteomic Analysis of Isopsoralen Protection against Oxidative Damage in Human Lens Epithelial Cells

Objective： To investigate the protective effects of the natural medicinal monomer isopsoralen （ISR） with estrogenic activity against oxidative damage in human lens epithelial cells B3 （HLE-B3） caused by hydrogen peroxide （H2O2） and to pursue the possible mitochondrial proteomic regularity of the protective effects. Methods： HLE-B3 cells were treated with H2O2 （300 μMol/L）, β-estradiol （E2：10^-8mol/L） and H2O2, ISR （10^-5 mol/L） and H2O2, or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surface- enhanced laser desorpUon ionization time of flight mass spectrometry （SELDI-TOF-MS）. The mass/charge （m/z） ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. Results： H2O2 up-regulated the expressions of two protein spots （with m/z of 6532 and 6809）. E2 mitigated the oxidative damage, and the expression of one protein spot （m/z 6532） was down-regulated. In contrast, ISR down-regulated both of protein spots （m/z 6532 and 6809）. Conclusions： ISR could effectively inhibit H2O2-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H2O2.