Preliminary observations on the result of infection by parvovirus of primary cultured cells of human hepatoma and parahepatoma tissue were reported. Human hepatoma and parahepatoma tissue were obtained by the culture ...Preliminary observations on the result of infection by parvovirus of primary cultured cells of human hepatoma and parahepatoma tissue were reported. Human hepatoma and parahepatoma tissue were obtained by the culture method of tissue fragment plating. It was observed that their respective sensitivity to parvovirus H-1 was different. H-1 had inhibitory effect on epithelial cells of hepatoma tissue but without any significant effect on fibroblasts simultaneously, H-l had no effect on epithelial cells and fibroblasts of parahepatoma tissue. Further investigation of the relationship between parvovirus and hepatoma would be helpful not only in elucidating the mechanism of carcinogenesis and its suppression but also in the search of new approach for the treatment of hepatoma.展开更多
One mM KCN fully inhibited the activity of cytochrome oxidase and the succinateoxidation in corn mitochondris, while the oxygen uptake of α-ketoglutarate was not affectedat all. In the latter case, a very strong inhi...One mM KCN fully inhibited the activity of cytochrome oxidase and the succinateoxidation in corn mitochondris, while the oxygen uptake of α-ketoglutarate was not affectedat all. In the latter case, a very strong inhibition could occur when both KCN and KSCNwere simultaneously present. After cyanide inhibition of succinate oxidation, the oxygen uptake could subsequentlyreappear by addition of α-ketoglutarate, but at that time the cytochromes c and a stillremained in their reduced state. In contrast to the cyanide-resistance,α-ketoglutarate oxidation was inhibited by antimycin A. It has been suggested that cytochromes a and c are not involved in the cyanide-insensi-tive alternative pathway in corn mitochondria and the branching point is located on the mainrespiratory chain after cytochrome b instead of before it.展开更多
文摘Preliminary observations on the result of infection by parvovirus of primary cultured cells of human hepatoma and parahepatoma tissue were reported. Human hepatoma and parahepatoma tissue were obtained by the culture method of tissue fragment plating. It was observed that their respective sensitivity to parvovirus H-1 was different. H-1 had inhibitory effect on epithelial cells of hepatoma tissue but without any significant effect on fibroblasts simultaneously, H-l had no effect on epithelial cells and fibroblasts of parahepatoma tissue. Further investigation of the relationship between parvovirus and hepatoma would be helpful not only in elucidating the mechanism of carcinogenesis and its suppression but also in the search of new approach for the treatment of hepatoma.
文摘One mM KCN fully inhibited the activity of cytochrome oxidase and the succinateoxidation in corn mitochondris, while the oxygen uptake of α-ketoglutarate was not affectedat all. In the latter case, a very strong inhibition could occur when both KCN and KSCNwere simultaneously present. After cyanide inhibition of succinate oxidation, the oxygen uptake could subsequentlyreappear by addition of α-ketoglutarate, but at that time the cytochromes c and a stillremained in their reduced state. In contrast to the cyanide-resistance,α-ketoglutarate oxidation was inhibited by antimycin A. It has been suggested that cytochromes a and c are not involved in the cyanide-insensi-tive alternative pathway in corn mitochondria and the branching point is located on the mainrespiratory chain after cytochrome b instead of before it.
