Application of the extended Fourier amplitude sensitivity testing(FAST)method to inflated,axial stretched,and residually stressed cylinders

This paper is dedicated to applying the Fourier amplitude sensitivity test(FAST)method to the problem of mixed extension and inflation of a circular cylindrical tube in the presence of residual stresses.The metafunctions and the Ishigami function are considered in the sensitivity analysis(SA).The effects of the input variables on the output variables are investigated,and the most important parameters of the system under the applied pressure and axial force such as the axial stretch and the azimuthal stretch are determined.

An Improvement of the Mass Flux Convection Parameterization Scheme and its Sensitivity Tests for Seasonal Prediction over China

A modified cumulus parameterization scheme, suitable for use in a seasonal forecast model, is presented. This parameterization scheme is an improvement of the mass flux convection scheme developed by Gregory and Rowntree (1989; 1990). This convection scheme uses a 'bulk' cloud model to present an ensemble of convective clouds, and aims to represent shallow, deep, and mid-level convection. At present, this convection scheme is employed in the NCC T63L20 model (National Climate Center, China Meteorological Administration). Simulation results with this scheme have revealed some deficiencies in the scheme, although to some extent, it improves the accuracy of the simulation. In order to alleviate the deficiencies and reflect the effect of cumulus convection in the actual atmosphere, the scheme is modified and improved. The improvements include (i) the full estimation of the effects of the large-scale convergence in the lower layer upon cumulus convection, (ii) the revision of the initial convective mass flux, and (iii) the regulation of convective-scale downdrafts. A comparison of the results obtained by using the original model and the modified one shows that the improvement and modification of the original convection scheme is successful in simulating the precipitation and general circulation field, because the modified scheme provides a good simulation of the main features of seasonal precipitation in China, and an analysis of the anomaly correlation coefficient between the simulation and the observations confirms the improved results.

Isolation and Identification of Pseudomonas solanacearum and Its Drug Sensitivity Test

Ten pathogenic strains were isolated fi-om gingers infected by blast, and were identified by substrate utilization test and biochemical test. The identifica- tion results showed that these ten strains accorded with the basic characteristics of Pseudomonas solanacearum. Drug sensitivity test of ten strains was carried out, and prevention agents were screened to provide an experimental basis for the control of ginger blast.

Drug Sensitivity Test and Regression Verification of Escherichia coli from Rex Rabbit

[ Objectives] The paper aimed to select drugs reasonably for treatment of rex rabbit colibacillosis, and to isolate the pathogenicity of Escherichia coll. [ Methods ] Pathogen isolation, drug sensitivity test and pathogen regression test were performed with rex rabbits killed by E. coli in clinic. [ Results] The isolate was E. coli 0-23, susceptible to amikacin and cefotaxime sodium; when the challenge dose was 1.0 mL/rabbit （about one billion E. coli）, the test animal would discharge mucous feces. [ Conclusions] The results provided model support for clinical medicine selection against rex rabbit colibacillosis.

Isolation, Identification and Drug Sensitivity Test of a Pathogenic Escherichia coli Strain from Minks with Diarrhea

[Objectives] The study aimed to identify the pathogen that causes diarrhea in minks. [Methods] Liver tissues were aseptically collected from dead minks with diarrhea. By bacterial isolation and culture,morphological observation,biochemical test and pathogenicity test,the isolated strain was identified as pathogenic E. coli. [Results]The pathogen causing diarrhea in minks was confirmed as a pathogenic E. coli strain. Drug sensitivity test indicated that the isolated pathogenic E. coli strain was highly sensitive to ceftazidime,cefotaxime,enrofloxacin,florfenicol and cephradine,moderately sensitive to ampicillin,ciprofloxacin,amikacin,doxycycline,lincomycin and gentamycin,and resistant to amoxycillin,neomycin,spectinomycin,polymyxin and penicillin. [Conclusions] This study provided reference for the prevention and control of abortion in female minks in Qinhuangdao region.

The Effect of the Atmospheric and Cloud Properties on the Retrieval of Fog Microphysics at Night for MSG/SEVIRI: A Sensitivity Test Using LibRadtran

The simplest way of building a look-up table (LUT) for the retrieval of cloud microphysical properties is to use a standard atmospheric profile and vertically uniform cloud microphysics. Such an assumption has been demonstrated to be incoherent with in-cloud observations. This paper aims to show the effect of some atmospheric conditions associated with fog as well as its macro-and microstructure on brightness temperature (BT) for the MSG/ SEVIRI satellite using libRadtran. The sensitivity tests were performed by gradually changing some features from the initial data, such as cloud cover, total water vapor column, thermal inversion intensity, fog depth, fog microstructure, and others. The results revealed that some variables can cause significant variations on BT and, consequently, discrepancies in the retrieval of fog microphysical properties. Also, a variation as high as 0.5°C was found on BT just by switching uniform to the non-uniform profile of fog microphysics depending on the channel, the droplet size, and optical thickness.

Distribution of pathogenic bacteria and antimicrobial sensitivity of eye infections in Suzhou

AIM:To investigate the types of bacteria in patients with eye infections in Suzhou and their drug resistance to commonly used antibacterial drugs.METHODS:The clinical data of 155 patients were retrospectively collected in this study,and the pathogenic bacteria species and drug resistance of each pathogenic bacteria were analyzed.RESULTS:Among the 155 patients(age from 12 to 87 years old,with an average age of 57,99 males and 56 females)with eye infections(160 eyes:74 in the left eye,76 in the right eye and 5 in both eyes,all of which were exogenous),71(45.81%)strains were gram-positive bacteria,23(14.84%)strains were gram-negative bacteria and 61(39.35%)strains were fungi.Gram-positive bacteria were highly resistant to penicillin and erythromycin(78.87%and 46.48%respectively),but least resistant to vancomycin at 0.Gram-negative bacteria were highly resistant to cefoxitin and compound sulfamethoxazole(100%and 95.65%respectively),but least resistant to meropenem at 0.Comparison of the resistance of gram-positive and gram-negative bacteria to some drugs revealed statistically significant differences(P<0.05)in the resistance of both to cefoxitin,cotrimoxazole,levofloxacin,cefuroxime,ceftriaxone and ceftazidime,and both had higher rates of resistance to gram-negative bacteria than to gram-positive bacteria.The distribution of bacterial infection strains showed that Staphylococcus epidermidis was the most common strain in the conjunctiva,cornea,aqueous humor or vitreous body and other eye parts.Besides,Fusarium and Pseudomonas aeruginosa were also among the most common strains of conjunctival and corneal infections.CONCLUSION:Gram-positive bacteria are the dominant bacteria in eye infections,followed by gram-negative bacteria and fungi.Considering the resistance of gramnegative bacteria to multiple drugs,monitoring of bacteria should be strengthened in eye bacterial infections for effective prevention and control to reduce complications caused by eye infections.

Six-year analysis of key monitoring for bacterial strain distribution and antibiotic sensitivity in a hospital

BACKGROUND With the widespread use of antimicrobial drugs,bacterial resistance has become a significant problem,posing a serious threat to public health.The prevalence of clinical infection strains in hospitals and their drug sensitivities are key to the appropriate use of antibiotics in clinical practice.AIM To identify prevalent bacteria and their antibiotic resistance profiles in a hospital setting,thereby guiding effective antibiotic usage by clinicians.METHODS Specimens from across the institution were collected by the microbiology laboratory.The VITEK 2 compact fully automatic analyzer was used for bacterial identification and antibiotic sensitivity testing,and the WHONET5.6 software was utilized for statistical analysis.RESULTS A total of 12062 bacterial strains of key monitoring significance were detected.Staphylococcus aureus demonstrated widespread resistance to penicillin,but none of the strains were resistant to vancomycin or linezolid.Moreover,219 strains of methicillin-resistant coagulase-negative staphylococci and 110 strains of methicillin-resistant Staphylococcus aureus were detected.Enterococcus faecalis showed moderate resistance to the third-generation quinolones ciprofloxacin and levofloxacin,but its resistance to nitrofurantoin and tetracycline was low.Enterococcus faecium displayed significantly lower resistance to third-and fourthgeneration quinolones than Enterococcus faecalis.The resistance of two key monitoring strains,Escherichia coli and Klebsiella pneumoniae,to piperacillin/tazobactam was 5%-8%.However,none of the Escherichia coli and Klebsiella pneumoniae strains were resistant to meropenem.The resistance of Acinetobacter baumannii to piperacillin/sulbactam was nearly 90%.Nonetheless,the resistance to tigecycline was low,and Pseudomonas aeruginosa demonstrated minimal resistance in the antibiotic sensitivity test,maintaining a resistance of<10%to the cephalosporin antibiotics cefotetan and cefoperazone over the last 6 years.The resistance to amikacin remained at 0.2%over the past 3 years.CONCLUSION Our hospital’s overall antibiotic resistance rate was relatively stable from 2017 to 2022.The detection rates of key monitoring strains are reported quarterly and their resistance dynamics are monitored and communicated to the entire hospital,which can guide clinical antibiotic selection.

Age norms for grating acuity and contrast sensitivity measured by Lea tests in the first three years of life

AIM： To determine age norms in the first three years of life for grating visual acuity and contrast sensitivity obtained with Lea grating test and Hiding Heidi low contrast face test. METHODS： Lea grating test was used to estimate binocular grating acuity and Hiding Heidi low contrast face test was used to estimate contrast sensitivity in 600 healthy infants and children. Age ranged from 3 to 36 mo subdivided into 12 groups subjected for full ophthalmologic and pediatric examinations.RESULTS： The grating acuity developed along the first three years of life. It ranged from 1.88±0.32 c/d at 3mo to 30.95±0.77 c/d at 36 mo. The most rapid development was during the first 12 mo and the slowest development was from 30 to 36 mo. The contrast sensitivity showed rapid development in the first two years of life. Its mean value ranged from 4.23±1.17 at 3mo to 78.26±8.21 at 24 mo. It was constant at the highest score（80） thereafter.CONCLUSION： Age norms for grating acuity along with contrast sensitivity offer a more comprehensive measure of spatial vision and should be incorporated in clinical practice for better visual assessment in preverbal and nonverbal children.

The Changes of Baroreflex Sensitivity During Head-up Tilt Test and Its Clinical Significance in the Patients with Vasovagal Syncope

Objective To study thechanges of baroreflex sensitivity (BRS) during head - up tilt test (HUT) in patients with vasovagal syncope (VS), and to examine the relationship between baroreflex sensitivity and neurohormonal factors. Furthermore, to investigate the effects of the changes of BRS on VS. Methods Forty - two patients with unexplained syncope (Among the 42 patients, there were 22 patients with positive HUT and 20 patients with negative HUT respectively) and 20 healthy volunteers (with negative HUT) underwent passive head - up tilt testing, Ante-cubital vein blood samples were taken before and after HUT, or at syncope. The fasting plasma endothelin , serum nitric oxide (NO), serum NE were measured, the BRS was assessed on the basis of the linear regression slope the RR interval versus systolic arterial blood pressure during the increment in blood pressure after intravenous administration of phenylephrine. Results (1) During the syncope, the BRS significantly reduced in HUT(+) group than baseline. At the end of tilt, the level of plasma ET, serum NO in patients with positive HUT significantly increased compared with baseline or normal controls, and the plasma concentration of NE also had the trend of increase. (2) By multiple regression analysis, a significant negative correlation was found between baroreceptor sensitivity and the plasma ET, NO at the end of HUT in patients with positive HUT, but there was no relationship between BRS and NE. Conclusions During the syncope occure, the BRS in patients with VS decreased significantly compared with normal controls. The abnormal plasma ET, NO concen-tration might contribute to the mechanism of VS.

The Coordinated Influence of Indian Ocean Sea Surface Temperature and Arctic Sea Ice on Anomalous Northeast China Cold Vortex Activities with Different Paths during Late Summer

The Northeast China cold vortex(NCCV)during late summer(from July to August)is identified and classified into three types in terms of its movement path using machine learning.The relationships of the three types of NCCV intensity with atmospheric circulations in late summer,the sea surface temperature(SST),and Arctic sea ice concentration(SIC)in the preceding months,are analyzed.The sensitivity tests by the Community Atmosphere Model version 5.3(CAM5.3)are used to verify the statistical results.The results show that the coordination pattern of East Asia-Pacific(EAP)and Lake Baikal high pressure forced by SST anomalies in the North Indian Ocean dipole mode(NIOD)during the preceding April and SIC anomalies in the Nansen Basin during the preceding June results in an intensity anomaly for the first type of NCCV.While the pattern of high pressure over the Urals and Okhotsk Sea and low pressure over Lake Baikal during late summer-which is forced by SST anomalies in the South Indian Ocean dipole mode(SIOD)in the preceding June and SIC anomalies in the Barents Sea in the preceding April-causes the intensity anomaly of the second type.The third type is atypical and is not analyzed in detail.Sensitivity tests,jointly forced by the SST and SIC in the preceding period,can well reproduce the observations.In contrast,the results forced separately by the SST and SIC are poor,indicating that the NCCV during late summer is likely influenced by the coordinated effects of both SST and SIC in the preceding months.

Clinical Distribution and Drug Resistance of Acinetobacter baumannii in a Hospital from 2019 to 2021

Objective:To analyze the clinical distribution and drug resistance of Acinetobacter baumannii(AB)and provide reference for the treatment of AB infection.Methods:AB isolated from clinical specimens of Huaihua First People’s Hospital from 2019 to 2021 were collected and identified by VITEK 2 Compact,an automated microbial identification and susceptibility testing system,in which drug sensitivity test was also performed.Excel was used for statistical analysis.Results:Among the 1,311 AB strains,81.16%(1,064 strains)were from sputum samples,and the departments with the highest detections rates of AB were neurosurgery(24.33%),intensive care(15.48%)and infectious disease(11.44%).The drug sensitivity test showed that the resistance rate of 1,311 AB strains to compound sulfamethoxazole and amikacin was 28.38%and 20.54%,respectively,and the resistance rate to 10 other kinds of common antibiotics was more than 40%.Conclusion:The 1,311 AB strains isolated were widely distributed in clinical settings and had strong resistance to commonly used antibiotics.Therefore,it is necessary to strengthen the monitoring of pathogens and drug resistance,formulate reasonable and effective infection control measures,and ensure that antibiotics are used in a reasonable manner.

克拉-2异常高压气藏岩石应力敏感性测定及其对产能的影响(英文)

KeLa-2 gas reservoir is the largest uncompartimentalized gas field so far discovered in China, with a reserve of hundreds of billions of cubic meters of dry gas. It has such features as extremely long interval (550m), high pressure (74.5MPa) and pressure coefficient (2.022). Gas reservoirs with a pressure coefficient of over 2.0 are not commonly found. The abnormal high-pressure reservoirs are quite different in characteristic and performance during the process of depletion exploitation. Therefore, it is necessary to know the property of pressure sensitivity for this abnormal high-pressure reservoir. The aim of this paper is to test the reservoir pressure sensitivity and to analyze its effect on the deliverability of gas. Through some experiments, the permeability change with the confining pressure of rock samples from KeLa-2 abnormal high-pressure gas reservoir is measured. A power function is used to match the measured data, and to derive an empirical equation to describe the change of permeability through the change of the reservoir pressure or effective overburden pressure. Considering the permeability change during the development of reservoirs, a conventional deliverability equation is modified, and the deliverability curve for KeLa-2 gas reservoir is predicted. The research indicates that the extent of the pressure sensitivity of rock samples from KeLa-2 is higher than that from the Daqing oilfield. KeLa-2 reservoir rock has the feature of an undercompaction state. The pressure sensitivity of a reservoir may decrease the well deliverability. It is concluded that for KeLa-2 reservoir the predicted absolute open flow (AOF), when the pressure sensitivity is taken into account, is approximately 70% of the AOF when permeability is constant and does not change with pressure.

Numerical simulation based on two-directional freeze and thaw algorithm for thermal diffusion model

Freeze-thaw processes significantly modulate hydraulic and thermal char- acteristics of soil. The changes in the frost and thaw fronts （FTFs） affect the water and energy cycles between the land surface and the atmosphere. Thus, the frozen soil com- prising permafrost and seasonally frozen soil has important effects on the land surface hydrology in cold regions. In this study, a two-directional freeze and thaw algorithm is incorporated into a thermal diffusion equation for simulating FTFs. A local adaptive variable-grid method is used to discretize the model. Sensitivity tests demonstrate that the method is stable and FTFs can be tracked continuously. The FTFs and soil tempera- ture at the Qinghai-Tibet Plateau D66 site are simulated hourly from September 1, 1997 to September 22, 1998. The results show that the incorporated model performs much better in the soil temperature simulation than the original thermal diffusion equation, showing potential applications of the method in land-surface process modeling.

Relationship between Mutation of IR in the mtr System of Neisseria Gonorrhoeae and Multiple Antibiotic Resistance

To study the relationship between mutation of the inverted repeat sequence （IR） in the multiple transferable resistant system （mtr） of Neisseria gonorrhoeae （NG） and its multiple antibiotic resistance, minimal inhibitory concentrations （MICs） for the clinically isolated strains were tested by agar-dilution-method. The mtr system＇s IR gene of NG was sequenced after amplification by polymerase chain reaction （PCR）. Either two susceptive or five penicillin-resistant strains had no base mutation in IR gene, while all of the 13 strains with multiple-antibiotic-resistance had a singlebase deletion （A/T）. The result suggests that a single-base deletion of the thirteen-base IR sequence in mtr system of NG might result in multiple antibiotic resistance but is not associated with single antibiotic resistance.

Study on Possible Mechanism of Terrain Influence on Cold-flow Snowstorm

[Objective] The research aimed to study the possible mechanism of terrain effect on cold-flow snowstorm.[Method] By using the meso-scale numerical model(WRF),a cold-flow snowstorm weather process in Shandong Peninsula was carried out numerical simulation and terrain sensitivity contrast test.The possible reason of terrain effect on falling zone and strength of snowstorm was deeply analyzed from water vapor,thermodynamic field and so on.[Result] The mountain terrain in Shandong Peninsula had great influences on falling zone and strength of cold-flow snowstorm.The strength of snowstorm obviously increased,and the snowfall center obviously moved northward.The main reason was that terrain caused the low-level wind field convergence and vertical movement in the troposphere strengthened.Then,the spatial distribution of water vapor and snow water content in the cold-flow snowstorm process obviously changed.So,the whole snowstorm process was affected.[Conclusion] The mountain terrain in Shandong Peninsula was the important element which needed to be focused on considering in the forecast analysis of cold-flow snowstorm weather process.

Diagnostic Analyses and Numerical Modeling of Explosive Cyclone over Northwestern Pacific on January 11-13,2012

In this paper,we use FNL grid data obtained from the National Centers for Environmental Prediction(NCEP)to analyze an explosive cyclone(EC)that occurred over the northwestern Pacific Ocean from January 11 to 13,2012.To simulate the EC,we used the Weather Research and Forecasting model(WRFV3.5).The cyclone outbreak occurred east of Japan from January 11 to 12 and weakened near the Kamchatka Peninsula on January 13.The analysis results show a distinct frontal structure,in which the high potential vorticity(PV)of the upper troposphere extends downward to the surface,which can facilitate EC development.A low-level jet stream develops with the EC,which can lead to more distinct convergence.The results of sea surface temperature(SST)sensitivity tests suggest that changes in the SST can affect cyclone intensity,but have little effect on its path.When small changes are made to the SST,the air pressure at the cyclonic center responds more distinctly to an increased SST than a decreased SST.The results of our latent heat release test suggest that diabatic heating processes lead to maximum PV values in the lower troposphere.Latent heat is also one of the important factors influencing EC development.

Isolation and Characterization of E. Coli O157 : H7 from Infected Newborn Calves in Northeast China

Escherichia coli O157 : H7 is a foodborne pathogen that poses a major threat to public health. Epidemiologic investigations have identified dairy cows, especially calves, are the principal reservoir of E. coli O157 : H7. In this study, based on the results, E. coli O157 : H7 was the main cause of E. coli disease outbreak in late October, 2015, and more than 90% of newborn calves died of serious diarrhea. Through further experiments, the drug sensitivity and resistance of the strain, the expression of the virulence gene and virulence pathogenicity were studied. E. coli O157 : H7 isolates were resistant to 12 antibiotics including penicillin, tetracycline and ampicillin, and were sensitive to eight antibiotics including cefoperazone, ceftazidime and amikacin. Resistance genes included tetB, strB, aadB, aphA, floR, TEM and virulence genes included stx1, eaeA and hlyA. Using specific pathogen free mice, the result showed that the isolate was pathogenic with a median lethal dose of 7.9×107 CFU · mL-1. This study described the pathogenesis and clinical manifestations of E. coli O157 : H7 infection. These results guided the use of antibiotics in prevent and control of bacterial infections in the future.

Monitoring and Analysis on Multi Drug Resistance of Escherichia coli from Captive Population Amur Tiger

In order to investigate the multi drug resistance to Escherichia coli from captive population Amur tiger,E. coli strains were isolated from the fecal samples of tiger in Heilongjiang Amur Tiger Park in Harbin. The sensitivity of E. coli isolates to 14 antibiotics was determined by scrip diffusion method. The results indicated that all the isolates varied in drug resistance to different antibiotics; the isolates gave high resistance to ampicillin,with a drug fast rate of 100%; over80% of the isolates were resistant to tetracycline and Paediatric Compound Sulfamethoxazole Tablets(SMZ- TMP),and over 70% of the isolates were sensitive to aztreonam,amoxicillin /potassium clavulanate. Most of the isolates had high sensitive to aztreonam and amoxicillin / clavulanate acid.

Isolation,Identification and Drug Tolerance Analysis of Pathogenic Staphylococcus aureus from Broilers

White feather broilers in some broiler farm in Chengde area developed arthritis and died acutely. In order to identify the pathogen inducing the disease and death of the white feather broilers, samples such as livers and joint pus were collected from dead broilers under sterile condition, and one pathogenic strain was isolated. The isolate was identified to be Staphylococcus aureus through isolated culture, morphologic observation, inspection of biochemical property and animal test. The drug sensitivity test showed that the isolate was sensitive to ceftazidime, enrofloxacin, ceftriaxone, lincomycin and amikacin, but resistant to other drugs to different degrees.