目的为青牛胆的物种鉴定和遗传图谱的构建寻找一种新的途径。方法采用序列相关扩增多态性(Sequence related amplified polymorphism,SRAP)技术对青牛胆DNA进行PCR扩增,逐级优化反应参数。结果优化得到稳定重复性好的青牛胆SRAP反应体...目的为青牛胆的物种鉴定和遗传图谱的构建寻找一种新的途径。方法采用序列相关扩增多态性(Sequence related amplified polymorphism,SRAP)技术对青牛胆DNA进行PCR扩增,逐级优化反应参数。结果优化得到稳定重复性好的青牛胆SRAP反应体系。结论SRAP技术在分子水平上对青牛胆进行鉴定是一种行之有效的手段,为今后进一步的青牛胆物种鉴定、遗传图谱的构建等研究奠定基础。展开更多
A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific...A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers.展开更多
文摘目的为青牛胆的物种鉴定和遗传图谱的构建寻找一种新的途径。方法采用序列相关扩增多态性(Sequence related amplified polymorphism,SRAP)技术对青牛胆DNA进行PCR扩增,逐级优化反应参数。结果优化得到稳定重复性好的青牛胆SRAP反应体系。结论SRAP技术在分子水平上对青牛胆进行鉴定是一种行之有效的手段,为今后进一步的青牛胆物种鉴定、遗传图谱的构建等研究奠定基础。
基金This work was supported by the National"863"High Technology Foundation(Grant Nos.2001AA211121 and 2002AA-211031)the National"948"Foundation(Grant No.201012).
文摘A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers.
