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The Application of Nicotiana benthamiana as a Transient Expression Host to Clone the Coding Sequences of Plant Genes
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作者 Jianzhong Huang Peng Jia +3 位作者 Xiaoju Zhong Xiuying Guan Hongbin Zhang Honglei Ruan 《American Journal of Molecular Biology》 CAS 2024年第2期54-65,共12页
Coding sequences (CDS) are commonly used for transient gene expression, in yeast two-hybrid screening, to verify protein interactions and in prokaryotic gene expression studies. CDS are most commonly obtained using co... Coding sequences (CDS) are commonly used for transient gene expression, in yeast two-hybrid screening, to verify protein interactions and in prokaryotic gene expression studies. CDS are most commonly obtained using complementary DNA (cDNA) derived from messenger RNA (mRNA) extracted from plant tissues and generated by reverse transcription. However, some CDS are difficult to acquire through this process as they are expressed at extremely low levels or have specific spatial and/or temporal expression patterns in vivo. These challenges require the development of alternative CDS cloning technologies. In this study, we found that the genomic intron-containing gene coding sequences (gDNA) from Arabidopsis thaliana, Oryza sativa, Brassica napus, and Glycine max can be correctly transcribed and spliced into mRNA in Nicotiana benthamiana. In contrast, gDNAs from Triticum aestivum and Sorghum bicolor did not function correctly. In transient expression experiments, the target DNA sequence is driven by a constitutive promoter. Theoretically, a sufficient amount of mRNA can be extracted from the N. benthamiana leaves, making it conducive to the cloning of CDS target genes. Our data demonstrate that N. benthamiana can be used as an effective host for the cloning CDS of plant genes. 展开更多
关键词 Coding Sequence Genomic Sequence Nicotiana benthamiana Plant Genes
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Pig pangenome graph reveals functional features of non‑reference sequences
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作者 Jian Miao Xingyu Wei +6 位作者 Caiyun Cao Jiabao Sun Yuejin Xu Zhe Zhang Qishan Wang Yuchun Pan Zhen Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第3期956-970,共15页
Background The reliance on a solitary linear reference genome has imposed a significant constraint on our compre-hensive understanding of genetic variation in animals.This constraint is particularly pronounced for non... Background The reliance on a solitary linear reference genome has imposed a significant constraint on our compre-hensive understanding of genetic variation in animals.This constraint is particularly pronounced for non-reference sequences(NRSs),which have not been extensively studied.Results In this study,we constructed a pig pangenome graph using 21 pig assemblies and identified 23,831 NRSs with a total length of 105 Mb.Our findings revealed that NRSs were more prevalent in breeds exhibiting greater genetic divergence from the reference genome.Furthermore,we observed that NRSs were rarely found within coding sequences,while NRS insertions were enriched in immune-related Gene Ontology terms.Notably,our investigation also unveiled a close association between novel genes and the immune capacity of pigs.We observed substantial differences in terms of frequencies of NRSs between Eastern and Western pigs,and the heat-resistant pigs exhibited a substantial number of NRS insertions in an 11.6 Mb interval on chromosome X.Additionally,we discovered a 665 bp insertion in the fourth intron of the TNFRSF19 gene that may be associated with the ability of heat tolerance in South-ern Chinese pigs.Conclusions Our findings demonstrate the potential of a graph genome approach to reveal important functional features of NRSs in pig populations. 展开更多
关键词 Heat tolerance Immune ability Non-reference sequences Pig pangenome
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Phylogenetic, phylogeographic and divergence time analysis of Anopheles subpictus species complex using ITS2 and COI sequences
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作者 Lihini Sandaleka Muthukumarana Methsala Madurangi Wedage +1 位作者 Samanthika Rathnayake Nissanka Kolitha De Silva 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2024年第5期214-225,I0004-I0038,共47页
Objective:To address the phylogenetic and phylogeographic relationship between different lineages of Anopheles(An.)subpictus species complex in most parts of the Asian continent by maximum utilization of Internal Tran... Objective:To address the phylogenetic and phylogeographic relationship between different lineages of Anopheles(An.)subpictus species complex in most parts of the Asian continent by maximum utilization of Internal Transcriber Spacer 2(ITS2)and cytochrome C oxidase I(COI)sequences deposited at the GenBank.Methods:Seventy-five ITS2,210 COI and 26 concatenated sequences available in the NCBI database were used.Phylogenetic analysis was performed using Bayesian likelihood trees,whereas median-joining haplotype networks and time-scale divergence trees were generated for phylogeographic analysis.Genetic diversity indices and genetic differentiation were also calculated.Results:Two genetically divergent molecular forms of An.subpictus species complex corresponding to sibling species A and B are established.Species A evolved around 37-82 million years ago in Sri Lanka,India,and the Netherlands,and species B evolved around 22-79 million years ago in Sri Lanka,India,and Myanmar.Vietnam,Thailand,and Cambodia have two molecular forms:one is phylogenetically similar to species B.Other forms differ from species A and B and evolved recently in the above mentioned countries,Indonesia and the Philippines.Genetic subdivision among Sri Lanka,India,and the Netherlands is almost absent.A substantial genetic differentiation was obtained for some populations due to isolation by large geographical distances.Genetic diversity indices reveal the presence of a long-established stable mosquito population,at mutation-drift equilibrium,regardless of population fluctuations.Conclusions:An.subpictus species complex consists of more than two genetically divergent molecular forms.Species A is highly divergent from the rest.Sri Lanka and India contain only species A and B. 展开更多
关键词 Molecular systematics ITS2 COI DNA sequences Phylogeny PHYLOGEOGRAPHY
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On power series statistical convergence and new uniform integrability of double sequences
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作者 Sevda Y■ld■z Kamil Demirci 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2024年第3期519-532,共14页
In the present paper,we mostly focus on P_(p)^(2)-statistical convergence.We will look into the uniform integrability via the power series method and its characterizations for double sequences.Also,the notions of P_(p... In the present paper,we mostly focus on P_(p)^(2)-statistical convergence.We will look into the uniform integrability via the power series method and its characterizations for double sequences.Also,the notions of P_(p)^(2)-statistically Cauchy sequence,P_(p)^(2)-statistical boundedness and core for double sequences will be described in addition to these findings. 展开更多
关键词 power series methods statistical convergence uniform integrability double sequences
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Adaptive Successive POI Recommendation via Trajectory Sequences Processing and Long Short-Term Preference Learning
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作者 Yali Si Feng Li +3 位作者 Shan Zhong Chenghang Huo Jing Chen Jinglian Liu 《Computers, Materials & Continua》 SCIE EI 2024年第10期685-706,共22页
Point-of-interest(POI)recommendations in location-based social networks(LBSNs)have developed rapidly by incorporating feature information and deep learning methods.However,most studies have failed to accurately reflec... Point-of-interest(POI)recommendations in location-based social networks(LBSNs)have developed rapidly by incorporating feature information and deep learning methods.However,most studies have failed to accurately reflect different users’preferences,in particular,the short-term preferences of inactive users.To better learn user preferences,in this study,we propose a long-short-term-preference-based adaptive successive POI recommendation(LSTP-ASR)method by combining trajectory sequence processing,long short-term preference learning,and spatiotemporal context.First,the check-in trajectory sequences are adaptively divided into recent and historical sequences according to a dynamic time window.Subsequently,an adaptive filling strategy is used to expand the recent check-in sequences of users with inactive check-in behavior using those of similar active users.We further propose an adaptive learning model to accurately extract long short-term preferences of users to establish an efficient successive POI recommendation system.A spatiotemporal-context-based recurrent neural network and temporal-context-based long short-term memory network are used to model the users’recent and historical checkin trajectory sequences,respectively.Extensive experiments on the Foursquare and Gowalla datasets reveal that the proposed method outperforms several other baseline methods in terms of three evaluation metrics.More specifically,LSTP-ASR outperforms the previously best baseline method(RTPM)with a 17.15%and 20.62%average improvement on the Foursquare and Gowalla datasets in terms of the Fβmetric,respectively. 展开更多
关键词 Location-based social networks adaptive successive point-of-interest recommendation long short-term preference trajectory sequences
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Secure Transmission of Compressed Medical Image Sequences on Communication Networks Using Motion Vector Watermarking
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作者 Rafi Ullah Mohd Hilmi bin Hasan +1 位作者 Sultan Daud Khan Mussadiq Abdul Rahim 《Computers, Materials & Continua》 SCIE EI 2024年第3期3283-3301,共19页
Medical imaging plays a key role within modern hospital management systems for diagnostic purposes.Compression methodologies are extensively employed to mitigate storage demands and enhance transmission speed,all whil... Medical imaging plays a key role within modern hospital management systems for diagnostic purposes.Compression methodologies are extensively employed to mitigate storage demands and enhance transmission speed,all while upholding image quality.Moreover,an increasing number of hospitals are embracing cloud computing for patient data storage,necessitating meticulous scrutiny of server security and privacy protocols.Nevertheless,considering the widespread availability of multimedia tools,the preservation of digital data integrity surpasses the significance of compression alone.In response to this concern,we propose a secure storage and transmission solution for compressed medical image sequences,such as ultrasound images,utilizing a motion vector watermarking scheme.The watermark is generated employing an error-correcting code known as Bose-Chaudhuri-Hocquenghem(BCH)and is subsequently embedded into the compressed sequence via block-based motion vectors.In the process of watermark embedding,motion vectors are selected based on their magnitude and phase angle.When embedding watermarks,no specific spatial area,such as a region of interest(ROI),is used in the images.The embedding of watermark bits is dependent on motion vectors.Although reversible watermarking allows the restoration of the original image sequences,we use the irreversible watermarking method.The reason for this is that the use of reversible watermarks may impede the claims of ownership and legal rights.The restoration of original data or images may call into question ownership or other legal claims.The peak signal-to-noise ratio(PSNR)and structural similarity index(SSIM)serve as metrics for evaluating the watermarked image quality.Across all images,the PSNR value exceeds 46 dB,and the SSIM value exceeds 0.92.Experimental results substantiate the efficacy of the proposed technique in preserving data integrity. 展开更多
关键词 Block matching algorithm(BMA) compression full-search algorithm motion vectors ultrasound image sequence WATERMARKING
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A Novel Method of Deinterleaving Radar Pulse Sequences Based on a Modified DBSCAN Algorithm 被引量:2
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作者 Abolfazl Dadgarnia Mohammad Taghi Sadeghi 《China Communications》 SCIE CSCD 2023年第2期198-215,共18页
A modified DBSCAN algorithm is presented for deinterleaving of radar pulses in modern EW environments.A main characteristic of the proposed method is that using only time of arrival of pulses,the method can sort the p... A modified DBSCAN algorithm is presented for deinterleaving of radar pulses in modern EW environments.A main characteristic of the proposed method is that using only time of arrival of pulses,the method can sort the pulses efficiently.Other PDW information such as rise time,carrier frequency,pulse width,modulation on pulse,fall time and direction of arrival are not required.To identify the valid PRIs in a set of interleaved pulses,an innovative modification of the DBSCAN algorithm is introduced which is accurate and easy to implement.The proposed method determines valid PRIs more accurately and neglects the spurious ones more efficiently as compared to the classical histogram based algorithms such as SDIF.Furthermore,without specifying any input parameter,the proposed method can deinterleave radar pulses while up to 30%jitter is present in the associated PRI.The accuracy and efficiency of the proposed method are verified by computer simulations and real data results.Experimental simulations are based on different real and operational scenarios where the presence of missing and spurious pulses are also considered.So,the simulation results can be of practical significance. 展开更多
关键词 DEINTERLEAVING radar pulse sequences density based clustering pulse descriptor word
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Complicated expansion trajectories of insertion sequences and potential association with horizontal transfer of Wolbachia DNA
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作者 Yun-Heng Miao Da-Wei Huang Jin-Hua Xiao 《Zoological Research》 SCIE CAS CSCD 2023年第2期273-275,共3页
DEAR EDITOR,Insertion sequences(ISs) are the simplest structural transposable elements(TEs) in prokaryotes, consisting only of a transposase coding sequence and its bilateral short terminal inverted repeats. Due to th... DEAR EDITOR,Insertion sequences(ISs) are the simplest structural transposable elements(TEs) in prokaryotes, consisting only of a transposase coding sequence and its bilateral short terminal inverted repeats. Due to their gradually streamlined genomic construction, TEs rarely exist in the genomes of obligate endosymbionts. However, TE content, especially ISs. 展开更多
关键词 sequences INSERTION WOLBACHIA
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Identification of clade-wide putative cis-regulatory elements from conserved non-coding sequences in Cucurbitaceae genomes
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作者 Hongtao Song Qi Wang +2 位作者 Zhonghua Zhang Kui Lin Erli Pang 《Horticulture Research》 SCIE CSCD 2023年第4期238-248,共11页
Cis-regulatory elements regulate gene expression and play an essential role in the development and physiology of organisms.Many conserved non-coding sequences(CNSs)function as cis-regulatory elements.They control the ... Cis-regulatory elements regulate gene expression and play an essential role in the development and physiology of organisms.Many conserved non-coding sequences(CNSs)function as cis-regulatory elements.They control the development of various lineages.How-ever,predicting clade-wide cis-regulatory elements across several closely related species remains challenging.Based on the relationship between CNSs and cis-regulatory elements,we present a computational approach that predicts the clade-wide putative cis-regulatory elements in 12 Cucurbitaceae genomes.Using 12-way whole-genome alignment,we first obtained 632112 CNSs in Cucurbitaceae.Next,we identified 16552 Cucurbitaceae-wide cis-regulatory elements based on collinearity among all 12 Cucurbitaceae plants.Furthermore,we predicted 3271 potential regulatory pairs in the cucumber genome,of which 98 were verified using integrative RNA sequencing and ChIP sequencing datasets from samples collected during various fruit development stages.The CNSs,Cucurbitaceae-wide cis-regulatory elements,and their target genes are accessible at http://cmb.bnu.edu.cn/cisRCNEs_cucurbit/.These elements are valuable resources for functionally annotating CNSs and their regulatory roles in Cucurbitaceae genomes. 展开更多
关键词 elements CONSERVED sequences
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Leachate Lithium Characteristics of Loess-paleosol Sequences on the Chinese Loess Plateau and their Paleoclimatic Significance
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作者 JIA Peng FU Chaofeng +4 位作者 HE Maoyong LIU Junfeng LIU Na LI Yulong YANG Kaiyuan 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2023年第6期1814-1824,共11页
The geochemical components of the leachate from loess-paleosol deposits can provide information about climaterelated post-depositional processes.For example,leachate lithium([Li]_(leachate))is a potential paleoclimate... The geochemical components of the leachate from loess-paleosol deposits can provide information about climaterelated post-depositional processes.For example,leachate lithium([Li]_(leachate))is a potential paleoclimate proxy because lithium is a typical lithophile element that is readily adsorbed by clay minerals during weathering and pedogenesis,and thus stratigraphic variations in[Li]leachatecan reflect these processes.We investigated the[Li]leachatevalues of two loess-paleosols profiles(the Luochuan and Weinan sections),on a north-south climatic gradient on the Chinese Loess Plateau.Independent paleoclimate information was provided by measurements of magnetic susceptibility,grain size,Rb/Sr ratios,and clay mineral content.During the last glacial-interglacial period,[Li]leachateincreased from 0.39 to 1.97μg/g at Luochuan and from 0.67 to 2.45μg/g at Weinan,mainly due to increasing pedogenesis.Based on these results we developed a conceptual model to explain the variations in[Li]leachate,Li^(+)within loess layers is mainly derived from dust input and the decomposition of primary minerals,influenced by the East Asian winter monsoon,while in paleosol layers Li is mainly derived from clay mineral adsorption during pedogenic processes,influenced by the East Asian summer monsoon. 展开更多
关键词 leachate lithium concentration loess-paleosol sequences last glacial-interglacial cycle paleoclimate record
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About Upper and Lower Strong Fractional Weighted Mean Convergence by Moduli for Triple Sequences
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作者 Amelia Bucur 《Journal of Applied Mathematics and Physics》 2023年第5期1304-1318,共15页
In article, I present a study on upper and lower statistical convergence, and upper and lower strong fractional weighted mean convergence by moduli for triple sequences. One of the generalizations of the discrete oper... In article, I present a study on upper and lower statistical convergence, and upper and lower strong fractional weighted mean convergence by moduli for triple sequences. One of the generalizations of the discrete operator Cesàro, was weighted mean operators, which are linear operators, too. Given a modulus function f, I established that a triple sequence that is f-upper or lower strong fractional weighted mean convergent, in some supplementary conditions, is also f-lower or upper statistically convergent. The results of this paper adapt the results obtained in [1] and [2] to upper and lower strong fractional weighted mean convergence and to triple sequence concept. Furthermore, new concepts can be applied to the approximation theory, topology, Fourier analysis, analysis interdisciplinary with applications electrical engineering, robotics and other domains. 展开更多
关键词 Upper and Lower Statistical Convergence Triple sequences Unbounded Modulus Function Numerical Analysis
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The asymptotic relation between the first crossing point and the last exit time of Gaussian order statistics sequences
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作者 NING Zi-jun TAN Zhong-quan 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2023年第4期545-561,共17页
In this paper,we study the asymptotic relation between the first crossing point and the last exit time for Gaussian order statistics which are generated by stationary weakly and strongly dependent Gaussian sequences.I... In this paper,we study the asymptotic relation between the first crossing point and the last exit time for Gaussian order statistics which are generated by stationary weakly and strongly dependent Gaussian sequences.It is shown that the first crossing point and the last exit time are asymptotically independent and dependent for weakly and strongly dependent cases,respectively.The asymptotic relations between the first crossing point and the last exit time for stationary weakly and strongly dependent Gaussian sequences are also obtained. 展开更多
关键词 rst crossing point last exit time stationary Gaussian sequences Gaussian order statistic se-quences
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改进灰狼算法优化GBDT在PM_(2.5)预测中的应用 被引量:2
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作者 江雨燕 傅杰 +2 位作者 甘如美江 孙雨辰 王付宇 《安全与环境学报》 CAS CSCD 北大核心 2024年第4期1569-1580,共12页
针对灰狼算法易陷入局部最优解和全局搜索能力不足的问题,通过霍尔顿序列(Halton Sequence)搜索算法初始化狼群位置,避免灰狼算法陷入局部最优解和重复运算;引入莱维飞行和随机游动策略对灰狼算法的寻优过程进行优化,以增加算法的全局... 针对灰狼算法易陷入局部最优解和全局搜索能力不足的问题,通过霍尔顿序列(Halton Sequence)搜索算法初始化狼群位置,避免灰狼算法陷入局部最优解和重复运算;引入莱维飞行和随机游动策略对灰狼算法的寻优过程进行优化,以增加算法的全局搜索能力;利用粒子群算法模拟灰狼种群得出的最佳适应度以用于惩罚项改进灰狼算法中的头狼更新策略。使用改进算法优化的梯度提升树(Gradient Boosting Decision Trees,GBDT)模型对北京市大气污染物监测数据中PM_(2.5)质量浓度进行预测,采用3种评估函数对各模型以及混合模型预测效果得分进行评估。结果显示,本文改进的灰狼算法对梯度提升树的优化效果优于其他算法,均方根误差E RMS为6.65μg/m^(3),平均绝对值误差E MA为3.20μg/m^(3),拟合优度(R^(2))为99%,比传统灰狼算法优化结果的均方根误差减少了19.19μg/m^(3),平均绝对值误差降低了10.03μg/m^(3),拟合优度增加了9百分点;与霍尔顿序列和莱维飞行改进的(Levy Flight-Halton Sequence,LHGWO)相比,改进的灰狼算法预测得分的均方根误差降低了10.39μg/m^(3),平均绝对值误差减小了6.71μg/m^(3),拟合优度提高了5百分点。研究表明了预测模型优化的有效性,为未来城市改善空气质量提供了科学依据和技术支持。 展开更多
关键词 环境学 PM_(2.5)质量浓度预测 改进灰狼算法(GWO) 梯度提升树算法(GBDT) 莱维(Levy)飞行 霍尔顿序列(Halton Sequence) 粒子群算法(PSO)
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高通量测序技术在低频突变检测中的应用 被引量:2
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作者 栾洋 尤馨悦 杨劲 《遗传》 CAS CSCD 北大核心 2024年第2期126-139,共14页
体细胞突变的累积与衰老、肿瘤及多种疾病的发生密切相关。在正常组织细胞中,基因组中自发突变和诱发突变的变异等位基因频率极低,对这类低频突变的检测一直面临挑战。第二代和第三代高通量测序(next-generation sequencing,NGS)技术的... 体细胞突变的累积与衰老、肿瘤及多种疾病的发生密切相关。在正常组织细胞中,基因组中自发突变和诱发突变的变异等位基因频率极低,对这类低频突变的检测一直面临挑战。第二代和第三代高通量测序(next-generation sequencing,NGS)技术的出现,可以实现任意物种全基因组上变异的直接检测,克服传统突变检测技术的诸多局限性。但是常规NGS由于测序错误率较高从而限制了其在低频突变检测上的应用,基于分子一致性测序策略进行错误矫正的高准确性NGS测序技术作为有效的低频突变检测工具,有望在环境诱变剂的评价与研究、细胞与基因治疗药物风险评估、人群健康风险监测和生命科学基础研究领域发挥重要作用。本文对比经典突变检测方法,对基于NGS的低频突变检测技术研究进展进行综述,并对应用前景进行展望,以期为该技术的进一步开发、研究和在相关领域的应用提供参考。 展开更多
关键词 高通量测序技术 低频突变 分子一致性测序 致突变 风险评估
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ASE-ERNIE:一种基于ERNIE的中文指令动作序列生成方法 被引量:1
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作者 赵逢达 郭凡 +2 位作者 李贤善 门旭静 王彬 《计算机集成制造系统》 EI CSCD 北大核心 2024年第5期1745-1753,共9页
中文结构灵活多样,同样的语义有多种表达方式,在中文指令过长或存在多组动作的情况下,家庭服务机器人很难正确理解并做出响应。此外,家庭环境的中文指令解析研究在数据方面仍然稀缺。现有的方法不能准确提取出指令中包含的全部动作序列... 中文结构灵活多样,同样的语义有多种表达方式,在中文指令过长或存在多组动作的情况下,家庭服务机器人很难正确理解并做出响应。此外,家庭环境的中文指令解析研究在数据方面仍然稀缺。现有的方法不能准确提取出指令中包含的全部动作序列。由此提出一种基于ERNIE方法的动作序列提取(ASE-ERNIE)模型来提取中文指令中的可执行动作序列,该模型通过动作类型和参数之间的关系提取指令信息,将动作序列的提取工作转化为序列标注任务。在指令中存在多组动作并且存在多个参数的情况下,依然能够提取出全部动作序列。通过实验验证了所提方法的有效性,动作序列提取任务的F 1值达到了80.37%。 展开更多
关键词 指令解析 动作序列 ERNIE方法 序列标注
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抗虫转基因水稻及其杂交水稻对土壤微生物群落多样性与组成的影响 被引量:3
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作者 宋亚娜 陈在杰 +3 位作者 林艳 胡太蛟 吴明基 王锋 《中国生态农业学报(中英文)》 CSCD 北大核心 2024年第1期15-29,共15页
微生物是土壤物质循环与肥力演变的驱动者,其群落组成关系到土壤微生态系统的稳定与可持续性。对抗虫转基因水稻土壤微生物群落变化的研究是其环境安全性评价的重要内容。本研究基于细菌16S rRNA基因和真菌ITS基因的高通量测序,分析了... 微生物是土壤物质循环与肥力演变的驱动者,其群落组成关系到土壤微生态系统的稳定与可持续性。对抗虫转基因水稻土壤微生物群落变化的研究是其环境安全性评价的重要内容。本研究基于细菌16S rRNA基因和真菌ITS基因的高通量测序,分析了田间试验中抗虫转基因水稻‘MFB’及其转基因杂交水稻‘闽丰A/MFB’‘天丰A/MFB’和‘谷丰A/MFB’与非转基因常规水稻‘闽恢3301’及杂交水稻‘天优华占’的土壤微生物群落多样性与组成的差异,并得出以下结果。首先,与两个非转基因水稻品种相比,抗虫转基因水稻及转基因杂交水稻均能显著增产(P<0.05)。同时,高通量测序结果表明,除水稻成熟期的土壤真菌群落外,与‘闽恢3301’相比‘MFB’的土壤细菌或真菌群落的α-多样性指数Chao1、Observed_species和Shannon均有所提高,且分别在水稻成熟期或分蘖期达到显著性水平(P<0.05);水稻齐穗期转基因杂交水稻‘闽丰A/MFB’‘天丰A/MFB’和‘谷丰A/MFB’的土壤细菌及真菌群落的多样性指数Shannon均介于‘MFB’与‘天优华占’之间;微生物群落β-多样性分析结果表明,本田间试验中不同品种水稻土壤细菌或真菌的群落组成均没有显著差异。但与‘闽恢3301’相比,稻田土壤细菌中丰度最高的变形菌门的相对丰度在‘MFB’土壤中明显增加,且在水稻分蘖期及成熟期达显著水平(P<0.05),而土壤真菌中丰度最高的子囊菌门的相对丰度在‘MFB’土壤中明显减少,且在水稻分蘖期及齐穗期差异显著(P<0.05);水稻齐穗期‘闽丰A/MFB’‘天丰A/MFB’和‘谷丰A/MFB’的土壤变形菌门或子囊菌门的相对丰度也均介于‘MFB’与‘天优华占’之间。此外,通过对微生物群落的功能组成预测可见,随水稻生长,‘MFB’与‘闽恢3301’的土壤细菌群落功能组成间差异存在增大的趋势。综上所述,本研究田间试验中,抗虫转基因水稻及其转基因杂交水稻在增产的同时提高了稻田土壤细菌或真菌群落的多样性,改变了主要细菌或真菌种类的相对丰度,但对细菌或真菌的群落及功能组成的影响不显著。 展开更多
关键词 抗虫转基因水稻 细菌群落 真菌群落 高通量测序
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无创产前检测:迈向下一个十年 被引量:2
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作者 王彦林 《临床儿科杂志》 CAS CSCD 北大核心 2024年第1期15-19,共5页
无创产前基因检测(NIPT)是过去10余年来产前筛查领域的重大突破,是围产医学领域的引领技术。通过低深度全基因组测序分析孕妇外周血胎儿游离DNA(cffDNA),实现了高效的唐氏综合征产前筛查,同时可扩展应用于其他染色体非整倍体、拷贝数变... 无创产前基因检测(NIPT)是过去10余年来产前筛查领域的重大突破,是围产医学领域的引领技术。通过低深度全基因组测序分析孕妇外周血胎儿游离DNA(cffDNA),实现了高效的唐氏综合征产前筛查,同时可扩展应用于其他染色体非整倍体、拷贝数变异等常见胎儿基因组问题的筛查,实现胎儿疾病的早期无创检测。但是,NIPT依然需要更多循证医学证据支持,以迈向更为广阔的应用空间。文章从NIPT适应证选择、NIPT异常结果分析、NIPT结果报道与解读中的伦理学问题、NIPT在胎儿基因变异筛查中的应用以及非cffDNA来源NIPT技术开发等角度,探讨了NIPT目前面临的问题与未来的发展趋势。 展开更多
关键词 无创产前筛查 游离胎儿DNA 基因组测序 外显组测序
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蒙古扁桃叶绿体遗传特征分析 被引量:2
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作者 段春燕 王晓凌 《北方园艺》 CAS 北大核心 2024年第2期31-37,共7页
以古老孑遗植物蒙古扁桃为试材,基于Illumina HiSeq X^(Ten)平台测序,采用生物信息学分析方法,研究了蒙古扁桃叶绿体基因组特征和系统发育情况,以期为蒙古扁桃和近缘种物种鉴定、系统发育地位以及早春观赏植物和西北荒漠草地植物的育种... 以古老孑遗植物蒙古扁桃为试材,基于Illumina HiSeq X^(Ten)平台测序,采用生物信息学分析方法,研究了蒙古扁桃叶绿体基因组特征和系统发育情况,以期为蒙古扁桃和近缘种物种鉴定、系统发育地位以及早春观赏植物和西北荒漠草地植物的育种培育提供参考依据。结果表明:蒙古扁桃叶绿体基因组为四分体式结构。其蛋白编码基因共编码26428个密码子,有29种是偏好密码子。密码子UUU数目最多,达1034个;密码子GCG数目最少,为137个。编码亮氨酸的密码子数目最多(2637个),占总量的9.98%;编码色氨酸的密码子数目最少(531个)仅占到1.94%。根据设置参数,发现蒙古扁桃叶绿体基因组中串联重复有16个,SSR位点有31个。SSR大多位于基因间区IGS和LSC区域。SSR中未检测到三核苷酸重复。基于叶绿体全基因组序列数据发现蒙古扁桃与桃亚属的桃和甘肃桃亲缘关系较近。 展开更多
关键词 蒙古扁桃 叶绿体 密码子 重复序列 系统发育
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基于高通量测序技术分析不同窖龄窖泥真菌群落多样性与空间异质性 被引量:3
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作者 任海伟 李志娟 +7 位作者 刘美琪 蔡早宁 孙一帆 郭晓鹏 范文广 张丙云 李彦涛 尉军强 《食品科学》 EI CAS CSCD 北大核心 2024年第2期178-187,共10页
利用Illumina NovaSeq高通量测序、冗余分析和FUNGuild等方法对甘肃金徽酒10a和50a窖龄窖池及其不同空间位置窖泥的真菌群落结构、真菌菌群与理化因子之间的关系以及真菌功能预测等进行研究。结果表明,随着窖池深度的增加,10 a窖池窖泥... 利用Illumina NovaSeq高通量测序、冗余分析和FUNGuild等方法对甘肃金徽酒10a和50a窖龄窖池及其不同空间位置窖泥的真菌群落结构、真菌菌群与理化因子之间的关系以及真菌功能预测等进行研究。结果表明,随着窖池深度的增加,10 a窖池窖泥的多样性和丰富度呈现降低趋势,50 a窖池窖泥的多样性整体呈现升高趋势,而丰富度则呈现先降低后升高趋势,其中10a窖池窖壁上层的多样性和丰富度均显著高于其他位置(P<0.05),而50a窖池窖底层的多样性和丰富度均显著高于其他位置(P<0.05)。10a窖池窖壁层的多样性和丰富度显著高于50a窖池(P<0.05),而50a窖底层的多样性和丰富度明显高于10a窖池(P<0.05)。所有窖泥样品共检测到21个真菌门和520个真菌属,其中子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、被孢霉门(Mortierellomycota)和罗兹菌门(Rozellomycota)4个优势真菌门以及曲霉属(Aspergillus)和哈萨克斯坦酵母属(Kazachstania)等多数优势真菌属的相对丰度随窖池窖龄和空间位置的变化差异显著(P<0.05)。镰刀霉属(Fusarium)、Aspergillus、Kazachstania和红曲霉属(Monascus)与水分、腐殖质、K^(+)和Ca^(2+)含量呈正相关,枝孢菌属(Cladosporium)、维希尼克氏酵母属(Vishniacozyma)与pH值呈正相关。窖泥真菌营养类型主要有腐生营养型和病理-腐生-共生营养型等7种,功能类群包括4个单一功能群和7个混合功能群。通过系统分析不同窖龄窖池和空间位置的窖泥真菌群落多样性,为明确甘肃金徽酒窖泥的真菌群落结构及空间分布规律奠定了理论基础。 展开更多
关键词 窖泥 高通量测序 真菌群落 冗余分析 功能预测
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煤系中关键金属元素的成矿作用研究进展与展望 被引量:7
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作者 代世峰 赵蕾 +2 位作者 王宁 魏强 刘晶晶 《矿物岩石地球化学通报》 CAS CSCD 北大核心 2024年第1期49-63,共15页
煤系中关键金属元素的赋存状态、分布规律、成矿机制以及有效提取和综合利用的研究是近10年来煤地质学等相关领域的研究前沿和热点问题,国内外学者在煤系中关键金属矿床的发现,关键金属元素赋存状态,成矿物质来源、迁移、富集和保存,开... 煤系中关键金属元素的赋存状态、分布规律、成矿机制以及有效提取和综合利用的研究是近10年来煤地质学等相关领域的研究前沿和热点问题,国内外学者在煤系中关键金属矿床的发现,关键金属元素赋存状态,成矿物质来源、迁移、富集和保存,开发利用潜力评价,精细勘查技术研发,关键元素的分离提取等方面取得了重要研究进展,为煤系关键金属矿产资源的开发利用奠定了坚实的理论和技术基础,同时在揭示与煤系关键金属相关重大地球科学问题方面取得了长足进展。本文结合国内外近些年来的进展,对煤系中关键金属元素(主要是锗、镓、锂、铌、锆等)的成矿作用进行了总结和凝练,指出了存在的问题及其解决方案和思路。 展开更多
关键词 煤系 关键金属 赋存状态 分布规律 成矿机制
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