To improve the performance of the short interleaved serial concatenated convolutional code(SCCC) with low decoding iterative times, the structure of Log MAP algorithm is introduced into the conventional SOVA decoder...To improve the performance of the short interleaved serial concatenated convolutional code(SCCC) with low decoding iterative times, the structure of Log MAP algorithm is introduced into the conventional SOVA decoder to improve its performance at short interleaving delay. The combination of Log MAP and SOVA avoids updating the matrices of the maximum path, and also makes a contribution to the requirement of short delay. The simulation results of several SCCCs show that the improved decoder can obtain satisfied performance with short frame interleaver and it is suitable to the high bit rate low delay communication systems.展开更多
A mine-used multi-function serial signal converter is introduced.This converter is based on Ethernet.The core of this design is embedded microprocessor STM32F107VCT6.Embedded operation system μC/OS-Ⅱ is transplanted...A mine-used multi-function serial signal converter is introduced.This converter is based on Ethernet.The core of this design is embedded microprocessor STM32F107VCT6.Embedded operation system μC/OS-Ⅱ is transplanted into this converter,and light-weight Internet protocal (LwIP) stack is also embedded to realize mutual conversion of serial signals such as meter bus (M-Bus) signal,RS485 signal,RS232 signal and Ethernet signal.Interconnection between all kinds of monitoring system interfaces under coal mine can be formed easily,which can solve compatibility problem between monitoring system and communication system and improve overall performance of safety monitoring system.The designed multi-function serial signal converter is of great value for application,which is worthy to be popularized in coal mine safety production.展开更多
The women of the 1930s in the Chinese TV serials deduct inspiring stories.They play different roles in the big wealthy families such as mothers-in-law,daughters-in-law,and maidservants.There are unceasing conflicts be...The women of the 1930s in the Chinese TV serials deduct inspiring stories.They play different roles in the big wealthy families such as mothers-in-law,daughters-in-law,and maidservants.There are unceasing conflicts between these women,because of their struggle for men's love,wealth or social position.The theme of their stories is always about fertility,love,kindness and evil,with the ending being either comic or tragic.The stories of various kinds of women of the 1930s in the Chinese TV serials decorate and enrich the lives of many of us latecomers and their names are passed on from generation to generation.展开更多
Empty categories,which lack phonological form and hence are not visible in the phonetic representation of the sentence,have both syntactic and semantic properties.Null subjects PRO and pro are base-generated empty cat...Empty categories,which lack phonological form and hence are not visible in the phonetic representation of the sentence,have both syntactic and semantic properties.Null subjects PRO and pro are base-generated empty categories.This thesis,based on Chomsky's theory about empty categories and research achievements made by Chinese scholars,attempts to analyze the liandong(Serial Verb) construction from Figure and Ground perspective.展开更多
The present study aimed to compare the complications and clinical outcomes of serial lumbar puncture(LP) and lumbar cerebrospinal fluid(CSF) drainage(LD) of patients with aneurysmal subarachnoid hemorrhage and p...The present study aimed to compare the complications and clinical outcomes of serial lumbar puncture(LP) and lumbar cerebrospinal fluid(CSF) drainage(LD) of patients with aneurysmal subarachnoid hemorrhage and provide more evidence to guide clinical management.In this retrospective study,41 and 39 aneurysmal subarachnoid hemorrhage patients were enrolled in the LP and LD group,respectively.Clinical outcomes,including CSF infection,intracerebral hemorrhage,vasospasm,hydrocephalus,death,length of stay,duration of drainage and the Glasgow Outcome Scale score were compared between the two groups.By comparing with the LP group,the LD group showed a significantly higher rate of CSF infection(P= 0.029) and shorter duration of drainage(P〈 0.001).Both groups displayed similar rates of vasospasm,hydrocephalus,intracerebral hemorrhage,the Glasgow Outcome Scale score one month after endovascular coiling and length of stay(P〉 0.05,respectively).In conclusion,both LD and serial LP are effective methods in the treatment of aneurysmal subarachnoid hemorrhage; besides,serial LP can reduce the incidence of CSF infection in draining hemorrhagic CSF in aneurysmal subarachnoid hemorrhage after endovascular coiling.展开更多
This paper presents a serial synchronous scanning mode in fiat panel display (FPD) by adding a latch buffer between the serializer and the driving buffer. Comparing with conventional techniques, the proposed structu...This paper presents a serial synchronous scanning mode in fiat panel display (FPD) by adding a latch buffer between the serializer and the driving buffer. Comparing with conventional techniques, the proposed structure can efficiently reduce the brightness loss and improve the transmission performance. Theoretical analysis and experimental results show that the ratio between the lightest weight display time and the relative transmission time is a tradeoff between brightness loss and transmission efficiency.展开更多
This paper introduces the usage of these functions. It presents the technique of how to program with them to perform the serial communications between a computer and other digital devices by the serial porst in Window...This paper introduces the usage of these functions. It presents the technique of how to program with them to perform the serial communications between a computer and other digital devices by the serial porst in Windows95. The related sample codes of the applications of this technique written in C++ programming language are also given. This technique is applied successfully to receive the data of the radio noise from a frequency monitor.展开更多
In order to guarantee safety and stability during physical human-robot-interaction(p HRI) in the occasion of service or industrial operation, a serial integrated rotary joint with the characteristics of passive and ac...In order to guarantee safety and stability during physical human-robot-interaction(p HRI) in the occasion of service or industrial operation, a serial integrated rotary joint with the characteristics of passive and active compliance is proposed. Passive compliance is achieved by a designed elastic element, such that the compliant joint may minimize large force which occurs during accidental impacts and, further, may offer more accurate and stable force control and a capacity for energy storage. Meanwhile, the modeling of the compliant joint is comprehensively analyzed, including the effect of the motor model on the overall control system. In order to realize the active compliance, a new method of impedance control is proposed. On the basis of PD control, a more compliant impedance controller is introduced. Experimental results show that the serial integrated rotary joint can provide more effective safety compliance during physical interaction, which has also been well applied in our designed massage robot and rehabilitation robot.展开更多
Present a kind of method which is used to communicate between serial serial port and peripheral equipment dynamicly and real-time using multithreading technique based on the basic principle of communication and multit...Present a kind of method which is used to communicate between serial serial port and peripheral equipment dynamicly and real-time using multithreading technique based on the basic principle of communication and multitasking mechanism in the circumstance of Windows. This method resolves the question of Real-time answering in the serial communication validly, reduces losing rate of data and improves reliability of system. This article presents a general method used in the serial communication which is practical.展开更多
In an industrial computer control system, if a communication with a single chip computer built with the aid of serial port I/O ports can be got easily to connect with equipment. Meanwhile, the system reliability would...In an industrial computer control system, if a communication with a single chip computer built with the aid of serial port I/O ports can be got easily to connect with equipment. Meanwhile, the system reliability would be improved and the system cost would be reduced.To save ±15 V power supply and electrical level convert circuit,the author provide a simple interface circuit and operational procedure between PC and the single chip computer in this paper. The application of multithread is introduced and the advantages of data acquisition and processing with multithread are also analyzed.展开更多
Differed from the existed applications of generalized stochastic Petri net(GSPN) theory in machine-tool manufacturing system, reliability computation of FMS, testabilityparameters determination and fault analysis, a n...Differed from the existed applications of generalized stochastic Petri net(GSPN) theory in machine-tool manufacturing system, reliability computation of FMS, testabilityparameters determination and fault analysis, a new idea of applying GSPN to model and performanceanalysis for the serial and parallel production system is proposed. And one typical discrete eventdynamic system (DEDS), turner-unit of palletizing system, is taken as a real case to research. Basedupon the established GSPN models, the working performances of serial and parallel layout arecompared. Furthermore, their differences of working mechanisms including feeding mechanism,coordinating mechanism and monitoring mechanism are discussed. Thus the theoretical basis which ishelpful to appraise layout plan and its reasonableness is provided. Meanwhile, the research resultsshow that parallel layout is more advantageous to greatly improve the operational speed ofproduction system than serial one.展开更多
Objective. To observe the clinical efficacy and mechanism of Zhuyu Tongfu (逐瘀通腑, ZYTF) Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage (HCH). Methods: One...Objective. To observe the clinical efficacy and mechanism of Zhuyu Tongfu (逐瘀通腑, ZYTF) Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage (HCH). Methods: One hundred and eighteen patients with hypertensive cerebral hemorrhage, on the basis of conventional Western medicine treatment, were randomly divided into ZYTF combined with acupuncture and massotherapy group (treated group) and simple Western medicine group (control group) ; the clinical efficacy, neurofunction deficit scoring (NDS) alterations and hematoma absorption rate of both groups were observed, and also the plasma superoxide dismutase (SOD) activity, plasma lipid peroxidase (LPO) content, erythrocyte glutathion peroxidase (GSH-Px) activity, hematocrit (Ht) and the whole blood viscosity (Va) change were also observed. Results: In the treated group, the clinical efficacy, NDS improvement and hematoma absorption rate were superior to that of the control group; comparison between the two groups after treatment showed that plasma SOD activity and GSH-Px activity got more elevated and plasma LPO content, Ht and Va more lowered in the the treated group than those in the control group. Cenclusien: ZYTF combined with acupuncture and massotherapy has better effect, its therapeutic mechanism was possibly correlated to the elevation of plasma SOD activity, GSH-Px activity and lowering of plasma LPO content, Ht and Va.展开更多
Data is a key asset for digital platforms,and mergers and acquisitions(M&As)are an important way for platform enterprises to acquire it.The types of data obtained from intra-industry and cross-sector M&As diff...Data is a key asset for digital platforms,and mergers and acquisitions(M&As)are an important way for platform enterprises to acquire it.The types of data obtained from intra-industry and cross-sector M&As differ,as does the extent to which they interact within or between platforms.The impact of such data on corporate market performance is an important question to consider when selecting strategies for digital platform M&As.Based on our research on advertising-driven platforms,we developed a two-stage Hotelling game model for comparing the market performance effects of intra-industry M&As and cross-sector M&As for digital platforms.We carried out an empirical test using relevant data from advertising-driven digital platforms between 2009 and 2021,as well as a case study on Baidu’s M&A activities.Our research discovered that intra-industry M&As driven by“data economies of scale”and cross-sector M&As driven by“data economies of scope”are both beneficial to the market performance of platform enterprises.Intra-industry M&As have a more significant positive effect on the market performance of platform enterprises because the same types of data are easier to integrate and develop the“network effect of data scale”.From a data factor perspective,this paper reveals the inherent economic logic by which different types of M&As influence the market performance of digital platforms,as well as policymaking recommendations for all digital platforms to select M&A strategies based on data scale,data scope,and the network effect of data.展开更多
BACKGROUND: It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution...BACKGROUND: It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE: To explore a semi-automatic computerized three-dimensional (3D) reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING: An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING: Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS: A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS: After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with "Curves", "Autocontrast", "Gray scale 8 bit", "Invert", "Image resize to 50%" steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES: The reconstructed human lumbar spinal cord segment. RESULTS: Compared with serial images obtained from other image modalities, such as CT, MRI, and macrosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION: A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord.展开更多
An optimal measurement pose number searching method was designed to improve the pose selection method.Several optimal robot measurement configurations were added to an initial pre-selected optimal configuration set to...An optimal measurement pose number searching method was designed to improve the pose selection method.Several optimal robot measurement configurations were added to an initial pre-selected optimal configuration set to establish a new configuration set for robot calibration one by one.The root mean squares (RMS) of the errors of each end-effector poses after being calibrated by these configuration sets were calculated.The optimal number of the configuration set corresponding to the least RMS of pose error was then obtained.Calibration based on those poses selected by this algorithm can get higher end-effector accuracy,meanwhile consumes less time.An optimal pose set including optimal 25 measurement configurations is found during the simulation.Tracking errors after calibration by using these poses are 1.54,1.61 and 0.86 mm,and better than those before calibration which are 7.79,7.62 and 8.29 mm,even better than those calibrated by the random method which are 2.22,2.35 and 1.69 mm in directions X,Y and Z,respectively.展开更多
A new microreactor with continuous serially connected micromixers(CSCM)was tailored for the coprecipitation process to synthesize Fe_(3)O_(4) nanoparticles.Numerical simulation reveals that the two types of CSCM micro...A new microreactor with continuous serially connected micromixers(CSCM)was tailored for the coprecipitation process to synthesize Fe_(3)O_(4) nanoparticles.Numerical simulation reveals that the two types of CSCM microchannels(V-typed and U-typed)proposed in this work exhibited markedly better mixing performances than the Zigzag and capillary microchannels due to the promotion of Dean vortices.Complete mixing was achieved in the V-typed microchannel in 2.7 s at an inlet Reynolds number of 27.Fe_(3)O_(4) nanoparticles synthesized in a planar glass microreactor with the V-typed microchannel,possessing an average size of 9.3 nm and exhibiting superparamagnetism,had obviously better dispersity and uniformity and higher crystallinity than those obtained in the capillary microreactor.The new CSCM microreactor developed in this work can act as a potent device to intensify the synthesis of similar inorganic nanoparticles via multistep chemical precipitation processes.展开更多
文摘To improve the performance of the short interleaved serial concatenated convolutional code(SCCC) with low decoding iterative times, the structure of Log MAP algorithm is introduced into the conventional SOVA decoder to improve its performance at short interleaving delay. The combination of Log MAP and SOVA avoids updating the matrices of the maximum path, and also makes a contribution to the requirement of short delay. The simulation results of several SCCCs show that the improved decoder can obtain satisfied performance with short frame interleaver and it is suitable to the high bit rate low delay communication systems.
文摘A mine-used multi-function serial signal converter is introduced.This converter is based on Ethernet.The core of this design is embedded microprocessor STM32F107VCT6.Embedded operation system μC/OS-Ⅱ is transplanted into this converter,and light-weight Internet protocal (LwIP) stack is also embedded to realize mutual conversion of serial signals such as meter bus (M-Bus) signal,RS485 signal,RS232 signal and Ethernet signal.Interconnection between all kinds of monitoring system interfaces under coal mine can be formed easily,which can solve compatibility problem between monitoring system and communication system and improve overall performance of safety monitoring system.The designed multi-function serial signal converter is of great value for application,which is worthy to be popularized in coal mine safety production.
文摘The women of the 1930s in the Chinese TV serials deduct inspiring stories.They play different roles in the big wealthy families such as mothers-in-law,daughters-in-law,and maidservants.There are unceasing conflicts between these women,because of their struggle for men's love,wealth or social position.The theme of their stories is always about fertility,love,kindness and evil,with the ending being either comic or tragic.The stories of various kinds of women of the 1930s in the Chinese TV serials decorate and enrich the lives of many of us latecomers and their names are passed on from generation to generation.
文摘Empty categories,which lack phonological form and hence are not visible in the phonetic representation of the sentence,have both syntactic and semantic properties.Null subjects PRO and pro are base-generated empty categories.This thesis,based on Chomsky's theory about empty categories and research achievements made by Chinese scholars,attempts to analyze the liandong(Serial Verb) construction from Figure and Ground perspective.
文摘The present study aimed to compare the complications and clinical outcomes of serial lumbar puncture(LP) and lumbar cerebrospinal fluid(CSF) drainage(LD) of patients with aneurysmal subarachnoid hemorrhage and provide more evidence to guide clinical management.In this retrospective study,41 and 39 aneurysmal subarachnoid hemorrhage patients were enrolled in the LP and LD group,respectively.Clinical outcomes,including CSF infection,intracerebral hemorrhage,vasospasm,hydrocephalus,death,length of stay,duration of drainage and the Glasgow Outcome Scale score were compared between the two groups.By comparing with the LP group,the LD group showed a significantly higher rate of CSF infection(P= 0.029) and shorter duration of drainage(P〈 0.001).Both groups displayed similar rates of vasospasm,hydrocephalus,intracerebral hemorrhage,the Glasgow Outcome Scale score one month after endovascular coiling and length of stay(P〉 0.05,respectively).In conclusion,both LD and serial LP are effective methods in the treatment of aneurysmal subarachnoid hemorrhage; besides,serial LP can reduce the incidence of CSF infection in draining hemorrhagic CSF in aneurysmal subarachnoid hemorrhage after endovascular coiling.
基金Project supported by the Science Foundation of Shanghai Municipal Commission of Science and Technology (Grant Nos.055207041, 047062012)
文摘This paper presents a serial synchronous scanning mode in fiat panel display (FPD) by adding a latch buffer between the serializer and the driving buffer. Comparing with conventional techniques, the proposed structure can efficiently reduce the brightness loss and improve the transmission performance. Theoretical analysis and experimental results show that the ratio between the lightest weight display time and the relative transmission time is a tradeoff between brightness loss and transmission efficiency.
基金Supported by the 86 3 High Technology Project of China (86 3-818-0 1-0 2 )
文摘This paper introduces the usage of these functions. It presents the technique of how to program with them to perform the serial communications between a computer and other digital devices by the serial porst in Windows95. The related sample codes of the applications of this technique written in C++ programming language are also given. This technique is applied successfully to receive the data of the radio noise from a frequency monitor.
基金Project(81473694)supported by the National Natural Science Foundation of ChinaProject(2016A1027)supported by the major Project of Zhongshan City,ChinaProject(2016FZFC007)supported by the Intelligent Equipment and Technology of Automation Research and Development Platform,China
文摘In order to guarantee safety and stability during physical human-robot-interaction(p HRI) in the occasion of service or industrial operation, a serial integrated rotary joint with the characteristics of passive and active compliance is proposed. Passive compliance is achieved by a designed elastic element, such that the compliant joint may minimize large force which occurs during accidental impacts and, further, may offer more accurate and stable force control and a capacity for energy storage. Meanwhile, the modeling of the compliant joint is comprehensively analyzed, including the effect of the motor model on the overall control system. In order to realize the active compliance, a new method of impedance control is proposed. On the basis of PD control, a more compliant impedance controller is introduced. Experimental results show that the serial integrated rotary joint can provide more effective safety compliance during physical interaction, which has also been well applied in our designed massage robot and rehabilitation robot.
文摘Present a kind of method which is used to communicate between serial serial port and peripheral equipment dynamicly and real-time using multithreading technique based on the basic principle of communication and multitasking mechanism in the circumstance of Windows. This method resolves the question of Real-time answering in the serial communication validly, reduces losing rate of data and improves reliability of system. This article presents a general method used in the serial communication which is practical.
文摘In an industrial computer control system, if a communication with a single chip computer built with the aid of serial port I/O ports can be got easily to connect with equipment. Meanwhile, the system reliability would be improved and the system cost would be reduced.To save ±15 V power supply and electrical level convert circuit,the author provide a simple interface circuit and operational procedure between PC and the single chip computer in this paper. The application of multithread is introduced and the advantages of data acquisition and processing with multithread are also analyzed.
基金This project is supported by National 863 Hi-Tech Project of China (No.512980106) Provincical Natural Science Foundation of Zhejiang (No.ZD0107).
文摘Differed from the existed applications of generalized stochastic Petri net(GSPN) theory in machine-tool manufacturing system, reliability computation of FMS, testabilityparameters determination and fault analysis, a new idea of applying GSPN to model and performanceanalysis for the serial and parallel production system is proposed. And one typical discrete eventdynamic system (DEDS), turner-unit of palletizing system, is taken as a real case to research. Basedupon the established GSPN models, the working performances of serial and parallel layout arecompared. Furthermore, their differences of working mechanisms including feeding mechanism,coordinating mechanism and monitoring mechanism are discussed. Thus the theoretical basis which ishelpful to appraise layout plan and its reasonableness is provided. Meanwhile, the research resultsshow that parallel layout is more advantageous to greatly improve the operational speed ofproduction system than serial one.
文摘Objective. To observe the clinical efficacy and mechanism of Zhuyu Tongfu (逐瘀通腑, ZYTF) Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage (HCH). Methods: One hundred and eighteen patients with hypertensive cerebral hemorrhage, on the basis of conventional Western medicine treatment, were randomly divided into ZYTF combined with acupuncture and massotherapy group (treated group) and simple Western medicine group (control group) ; the clinical efficacy, neurofunction deficit scoring (NDS) alterations and hematoma absorption rate of both groups were observed, and also the plasma superoxide dismutase (SOD) activity, plasma lipid peroxidase (LPO) content, erythrocyte glutathion peroxidase (GSH-Px) activity, hematocrit (Ht) and the whole blood viscosity (Va) change were also observed. Results: In the treated group, the clinical efficacy, NDS improvement and hematoma absorption rate were superior to that of the control group; comparison between the two groups after treatment showed that plasma SOD activity and GSH-Px activity got more elevated and plasma LPO content, Ht and Va more lowered in the the treated group than those in the control group. Cenclusien: ZYTF combined with acupuncture and massotherapy has better effect, its therapeutic mechanism was possibly correlated to the elevation of plasma SOD activity, GSH-Px activity and lowering of plasma LPO content, Ht and Va.
基金supported by the National Natural Science Foundation of China“Research on Cross-sector Competition Effect and Regulatory Policy of Digital Platforms Based on Inter-platform Network Externalities”(Grant No.72103085).
文摘Data is a key asset for digital platforms,and mergers and acquisitions(M&As)are an important way for platform enterprises to acquire it.The types of data obtained from intra-industry and cross-sector M&As differ,as does the extent to which they interact within or between platforms.The impact of such data on corporate market performance is an important question to consider when selecting strategies for digital platform M&As.Based on our research on advertising-driven platforms,we developed a two-stage Hotelling game model for comparing the market performance effects of intra-industry M&As and cross-sector M&As for digital platforms.We carried out an empirical test using relevant data from advertising-driven digital platforms between 2009 and 2021,as well as a case study on Baidu’s M&A activities.Our research discovered that intra-industry M&As driven by“data economies of scale”and cross-sector M&As driven by“data economies of scope”are both beneficial to the market performance of platform enterprises.Intra-industry M&As have a more significant positive effect on the market performance of platform enterprises because the same types of data are easier to integrate and develop the“network effect of data scale”.From a data factor perspective,this paper reveals the inherent economic logic by which different types of M&As influence the market performance of digital platforms,as well as policymaking recommendations for all digital platforms to select M&A strategies based on data scale,data scope,and the network effect of data.
基金Natural Science Research Plan forJiangsu Colleges, No.05KJB180105 Postgraduate Innovation Cultivating Projectin Jiangsu Province, No.CX07s_035z
文摘BACKGROUND: It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE: To explore a semi-automatic computerized three-dimensional (3D) reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING: An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING: Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS: A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS: After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with "Curves", "Autocontrast", "Gray scale 8 bit", "Invert", "Image resize to 50%" steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES: The reconstructed human lumbar spinal cord segment. RESULTS: Compared with serial images obtained from other image modalities, such as CT, MRI, and macrosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION: A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord.
基金Project(2008AA04Z203) supported by the National High Technology Research and Development Program of China
文摘An optimal measurement pose number searching method was designed to improve the pose selection method.Several optimal robot measurement configurations were added to an initial pre-selected optimal configuration set to establish a new configuration set for robot calibration one by one.The root mean squares (RMS) of the errors of each end-effector poses after being calibrated by these configuration sets were calculated.The optimal number of the configuration set corresponding to the least RMS of pose error was then obtained.Calibration based on those poses selected by this algorithm can get higher end-effector accuracy,meanwhile consumes less time.An optimal pose set including optimal 25 measurement configurations is found during the simulation.Tracking errors after calibration by using these poses are 1.54,1.61 and 0.86 mm,and better than those before calibration which are 7.79,7.62 and 8.29 mm,even better than those calibrated by the random method which are 2.22,2.35 and 1.69 mm in directions X,Y and Z,respectively.
基金the financial support from the National Natural Science Foundation of China(21808059)the Fundamental Research Funds for the Central Universities(JKA01221712).
文摘A new microreactor with continuous serially connected micromixers(CSCM)was tailored for the coprecipitation process to synthesize Fe_(3)O_(4) nanoparticles.Numerical simulation reveals that the two types of CSCM microchannels(V-typed and U-typed)proposed in this work exhibited markedly better mixing performances than the Zigzag and capillary microchannels due to the promotion of Dean vortices.Complete mixing was achieved in the V-typed microchannel in 2.7 s at an inlet Reynolds number of 27.Fe_(3)O_(4) nanoparticles synthesized in a planar glass microreactor with the V-typed microchannel,possessing an average size of 9.3 nm and exhibiting superparamagnetism,had obviously better dispersity and uniformity and higher crystallinity than those obtained in the capillary microreactor.The new CSCM microreactor developed in this work can act as a potent device to intensify the synthesis of similar inorganic nanoparticles via multistep chemical precipitation processes.