Objective:To assess the effect of sericin against pentylenetetrazole(PTZ)kindling epilepsy and its associated comorbidities.Methods:Epilepsy was induced with PTZ at the dose of 30 mg/kg i.p.on alternative days for 25 ...Objective:To assess the effect of sericin against pentylenetetrazole(PTZ)kindling epilepsy and its associated comorbidities.Methods:Epilepsy was induced with PTZ at the dose of 30 mg/kg i.p.on alternative days for 25 days in rats.Sericin was administered orally at the doses of 250,500,and 1000 mg/kg for 35 days.The behavioral activities were performed using an elevated plus maze,forced swim test,and Morris water maze test.A PTZ challenge test was conducted on day 32.On day 35,rats were sacrificed to perform oxidative stress,mitochondrial dysfunction,neuroinflammation,neurotransmitters,GABA-T activity,and histopathological analyses.Results:Sericin at 500 and 1000 mg/kg significantly reduced behavioral changes and neuroinflammatory cytokines,as well as improved oxidative stress,mitochondrial enzyme complex activity,neurotransmitter level,and GABA-T enzymatic activity(P<0.05).Moreover,sericin improved the neuronal survival altered by PTZ kindling in rat hippocampus.Conclusions:Sericin mitigates epilepsy-associated secondary complications possibly by the modulation of mitochondrial enzyme complexes and GABA-T enzymatic activity.展开更多
Cells are surrounded by a double-layered phospholipid cell membrane responsible for the isolation of intracellular contents, active regulation of uptake from the extracellular environment, and intercellular connection...Cells are surrounded by a double-layered phospholipid cell membrane responsible for the isolation of intracellular contents, active regulation of uptake from the extracellular environment, and intercellular connection and communication. These cell membranes must be intact and functionally active for cell survival and biological functioning. Compromised damage repair mechanisms usually result in impaired cellular homeostasis, leading to early or late problems. Chronic myopathies, certain myocardial diseases, aging, and acute or chronic neurodegenerative diseases (like Parkinson and Alzheimer) are directly related to cell membrane damage. This study examined the effect of a cholesterol-loaded nanoparticle (methyl-beta cyclodextrin) or the silk protein sericin on cell membrane and DNA integrity and cell viability in an in vitro cell damage model (frozen-thawed rabbit sperm cells). The cells were stored in liquid nitrogen (-196°C), thawed in small batches, and treated with cholesterol-loaded cyclodextrin or sericin before incubation at 35°C for 4 hours. Cell membrane integrity, DNA damage, and viability rates were assessed immediately after thawing and after the incubation period. The administration of sericin and cholesterol in a cell damage model increased cell survival and reduced DNA damage over a 4-hour post-thaw incubation period, suggesting their potential use as a “first aid” intervention at the cellular level.展开更多
针对SS304不锈钢水槽件在拉深过程中出现的零件起皱问题,采用正交试验方法研究凹模圆角半径、压边力和摩擦因数对起皱问题的影响。通过有限元分析软件Dynaform对正交试验方案进行仿真验证,考察了最大增厚率和凹模圆角半径、压边力和摩...针对SS304不锈钢水槽件在拉深过程中出现的零件起皱问题,采用正交试验方法研究凹模圆角半径、压边力和摩擦因数对起皱问题的影响。通过有限元分析软件Dynaform对正交试验方案进行仿真验证,考察了最大增厚率和凹模圆角半径、压边力和摩擦因数等3个成型影响因素之间的关系。试验结果表明,该SS304不锈钢盒型件的最佳成型工艺组合为R1C4F4,即:凹模圆角半径为5 m m,摩擦因数为0.2和压边力为50 kN。依据优化结果,改进了模具结构设计,从而较好地解决了SS304不锈钢盒型件的成型问题。展开更多
Previous studies have demonstrated that sericin effectively reduces blood glucose, and protects islet cells, as well as the gonads and kidneys. However, whether sericin improves diabetes mellitus-induced structural an...Previous studies have demonstrated that sericin effectively reduces blood glucose, and protects islet cells, as well as the gonads and kidneys. However, whether sericin improves diabetes mellitus-induced structural and functional problems in the central nervous system remains poorly understood. Rat models of type 2 diabetes mellitus were established by intraperitoneal injection of streptozotocin. The present study observed histological changes in the hippocampus and cerebral cortex, as well as heme oxygenase-1 expression, and explored sericin effects on the central nervous system in diabetic rats. Pathological damage to neural cells in the rat hippocampus and cerebral cortex was relieved following intragastric administration of sericin at a dose of 2.4 g/kg for 35 consecutive days. Heme oxygenase-1 protein and mRNA expressions were decreased in the hippocampus and cerebral cortex of diabetes mellitus rats after sericin treatment. The results suggest that sericin plays a protective effect on the nervous system by decreasing the high expression of heme oxygenase-1 following diabetes mellitus.展开更多
社会与情感能力是人生成功和幸福的奠基石。2021年SSES(Study on Social and Emotion-al Skills)全球首测结果发布,为开展青少年社会与情感能力的国际比较研究提供了坚实的数据基础。本文运用DEA(Data Envelope Analysis)方法,将班级规...社会与情感能力是人生成功和幸福的奠基石。2021年SSES(Study on Social and Emotion-al Skills)全球首测结果发布,为开展青少年社会与情感能力的国际比较研究提供了坚实的数据基础。本文运用DEA(Data Envelope Analysis)方法,将班级规模、教师学历、SSES培训、SSES措施、校园活动作为投入变量,将五大能力作为产出变量,对参加本次国际测评的10座城市的1171所中小学进行培养成效评估,结果发现:(1)各城市中小学的社会与情感能力培养成效普遍较高,小学阶段的均值在0.8以上,中学阶段的均值在0.9以上;(2)在小学阶段,苏州学校的培养成效较为突出,不仅均值最高,而且整体属于高水平均衡型;在中学阶段,培养成效较为突出的城市则是伊斯坦布尔,属于高水平均衡型;(3)各城市共有82所高绩效小学和87所高绩效中学,其中伊斯坦布尔和波哥大的占比较大;(4)学校归属感对各城市中小学的社会与情感能力培养成效提升具有重要促进作用。根据以上研究结果,本文建议应该加强社会与情感能力的国际合作与实证研究,在总结苏州成功经验的同时寻找差距补齐短板,将优质均衡作为我国中小学社会与情感能力培养工作的重要目标。展开更多
文摘Objective:To assess the effect of sericin against pentylenetetrazole(PTZ)kindling epilepsy and its associated comorbidities.Methods:Epilepsy was induced with PTZ at the dose of 30 mg/kg i.p.on alternative days for 25 days in rats.Sericin was administered orally at the doses of 250,500,and 1000 mg/kg for 35 days.The behavioral activities were performed using an elevated plus maze,forced swim test,and Morris water maze test.A PTZ challenge test was conducted on day 32.On day 35,rats were sacrificed to perform oxidative stress,mitochondrial dysfunction,neuroinflammation,neurotransmitters,GABA-T activity,and histopathological analyses.Results:Sericin at 500 and 1000 mg/kg significantly reduced behavioral changes and neuroinflammatory cytokines,as well as improved oxidative stress,mitochondrial enzyme complex activity,neurotransmitter level,and GABA-T enzymatic activity(P<0.05).Moreover,sericin improved the neuronal survival altered by PTZ kindling in rat hippocampus.Conclusions:Sericin mitigates epilepsy-associated secondary complications possibly by the modulation of mitochondrial enzyme complexes and GABA-T enzymatic activity.
文摘Cells are surrounded by a double-layered phospholipid cell membrane responsible for the isolation of intracellular contents, active regulation of uptake from the extracellular environment, and intercellular connection and communication. These cell membranes must be intact and functionally active for cell survival and biological functioning. Compromised damage repair mechanisms usually result in impaired cellular homeostasis, leading to early or late problems. Chronic myopathies, certain myocardial diseases, aging, and acute or chronic neurodegenerative diseases (like Parkinson and Alzheimer) are directly related to cell membrane damage. This study examined the effect of a cholesterol-loaded nanoparticle (methyl-beta cyclodextrin) or the silk protein sericin on cell membrane and DNA integrity and cell viability in an in vitro cell damage model (frozen-thawed rabbit sperm cells). The cells were stored in liquid nitrogen (-196°C), thawed in small batches, and treated with cholesterol-loaded cyclodextrin or sericin before incubation at 35°C for 4 hours. Cell membrane integrity, DNA damage, and viability rates were assessed immediately after thawing and after the incubation period. The administration of sericin and cholesterol in a cell damage model increased cell survival and reduced DNA damage over a 4-hour post-thaw incubation period, suggesting their potential use as a “first aid” intervention at the cellular level.
文摘针对SS304不锈钢水槽件在拉深过程中出现的零件起皱问题,采用正交试验方法研究凹模圆角半径、压边力和摩擦因数对起皱问题的影响。通过有限元分析软件Dynaform对正交试验方案进行仿真验证,考察了最大增厚率和凹模圆角半径、压边力和摩擦因数等3个成型影响因素之间的关系。试验结果表明,该SS304不锈钢盒型件的最佳成型工艺组合为R1C4F4,即:凹模圆角半径为5 m m,摩擦因数为0.2和压边力为50 kN。依据优化结果,改进了模具结构设计,从而较好地解决了SS304不锈钢盒型件的成型问题。
基金supported by the Grant of Department of Education of Hebei Province (GH/IGF-1 action mechanism in diabetes mellitus-induced gonadal axis injury and protective effects of sericin),No.2006301the Grant of the Department of Technology of Hebei Province (Protective effects of sericin on testicular dysfunction following diabetes mellitus),No.08276101D-19
文摘Previous studies have demonstrated that sericin effectively reduces blood glucose, and protects islet cells, as well as the gonads and kidneys. However, whether sericin improves diabetes mellitus-induced structural and functional problems in the central nervous system remains poorly understood. Rat models of type 2 diabetes mellitus were established by intraperitoneal injection of streptozotocin. The present study observed histological changes in the hippocampus and cerebral cortex, as well as heme oxygenase-1 expression, and explored sericin effects on the central nervous system in diabetic rats. Pathological damage to neural cells in the rat hippocampus and cerebral cortex was relieved following intragastric administration of sericin at a dose of 2.4 g/kg for 35 consecutive days. Heme oxygenase-1 protein and mRNA expressions were decreased in the hippocampus and cerebral cortex of diabetes mellitus rats after sericin treatment. The results suggest that sericin plays a protective effect on the nervous system by decreasing the high expression of heme oxygenase-1 following diabetes mellitus.
文摘社会与情感能力是人生成功和幸福的奠基石。2021年SSES(Study on Social and Emotion-al Skills)全球首测结果发布,为开展青少年社会与情感能力的国际比较研究提供了坚实的数据基础。本文运用DEA(Data Envelope Analysis)方法,将班级规模、教师学历、SSES培训、SSES措施、校园活动作为投入变量,将五大能力作为产出变量,对参加本次国际测评的10座城市的1171所中小学进行培养成效评估,结果发现:(1)各城市中小学的社会与情感能力培养成效普遍较高,小学阶段的均值在0.8以上,中学阶段的均值在0.9以上;(2)在小学阶段,苏州学校的培养成效较为突出,不仅均值最高,而且整体属于高水平均衡型;在中学阶段,培养成效较为突出的城市则是伊斯坦布尔,属于高水平均衡型;(3)各城市共有82所高绩效小学和87所高绩效中学,其中伊斯坦布尔和波哥大的占比较大;(4)学校归属感对各城市中小学的社会与情感能力培养成效提升具有重要促进作用。根据以上研究结果,本文建议应该加强社会与情感能力的国际合作与实证研究,在总结苏州成功经验的同时寻找差距补齐短板,将优质均衡作为我国中小学社会与情感能力培养工作的重要目标。