Genome-wide analysis of the SCPL gene family in grape(Vitis vinifera L.)

Serine carboxypeptidase-like(SCPL)proteins are a group of acyltransferase enzymes that have important roles in plant growth,development,and stress responses.Although SCPL proteins have been studied in many plants,the biological functions of SCPL genes in grape are still unknown.In this study,59 putative SCPL proteins were identified from the grape genome.A bioinformatics analysis,including chromosomal locations,exon/intron structures,phylogeny,cis-elements,and conserved motifs,was performed for the gene family.The phylogenetic analysis revealed that VvSCPL proteins could be classified into three groups,with the gene motifs in each group showing high similarity levels.The number of exons in the VvSCPL genes ranged from 1 to 19,suggesting significant variations among grape SCPL genes.The expression of the VvSCPL genes,as assessed by RNA sequencing(RNA-seq)and quantitative real-time PCR,showed that most VvSCPL genes responded to drought-and waterlogging-stress treatments,which indicated their roles in abiotic stress responses.The results provide useful information for further study of SCPL genes in grape.

植物丝氨酸羧肽酶样酰基转移酶的结构、功能和应用

植物丝氨酸羧肽酶样酰基转移酶与丝氨酸羧肽酶相比具有相似的结构特点和很高的同源性,能够催化酰基葡萄糖酯的酰基转移反应,参与植物次生代谢产物的酰基化修饰,丰富天然产物结构多样性,改善化合物水溶性、稳定性等理化性质。本文重点介绍植物来源丝氨酸羧肽酶样酰基转移酶家族的结构特点、催化机制、功能鉴定及其生物催化应用等方面的研究进展,为促进此类酰基转移酶基因的功能表征和利用合成生物技术合成活性次生代谢产物提供参考。