AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (D...AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (Deltapsim). METHODS: Cell culture, cell counting, ELISA assay, TUNEL, flow cytometry, Western blot and fluorometric assay were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanism. RESULTS: JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Caspases 8 and 9 were activated during apoptosis as judged by the appearance of cleavage products from procaspase and the caspase activities to cleave specific fluorogenic substrates. To elucidate whether the activation of caspases 8 and 9 was required for the apoptosis induction, we examined the effect of caspase-specific inhibitors on apoptosis. The results showed that caspase inhibitors significantly inhibited the apoptosis induced by JTE-522. In addition, the membrane translocation of Bax and cytosolic release of cytochrome C accompanying with the decrease of the uptake of Rhodamin 123, were detected at an early stage of apoptosis. Furthermore, Bax translocation, cytochrome C release, and caspase 9 activation were blocked by Z-VAD.fmk and Z-IETD-CHO. CONCLUSION: The present data indicate a crucial association between activation of caspases 8, 9, cytochrome C release, membrane translocation of Bax, loss of Deltapsim and JTE-522-induced apoptosis in AGS cells.展开更多
【目的】探讨血清胱抑素C（cystatinC，CysC）与老年2型糖尿病患者认知功能障碍的关系。【方法】选取老年2型糖尿病患者10。例，根据CysC水平分为高CysC组、低CysC组，比较两组各l临床特征及老年认知功能量表（scale of elderly cogniti...【目的】探讨血清胱抑素C（cystatinC，CysC）与老年2型糖尿病患者认知功能障碍的关系。【方法】选取老年2型糖尿病患者10。例，根据CysC水平分为高CysC组、低CysC组，比较两组各l临床特征及老年认知功能量表（scale of elderly cognitive function，SECF）得分的差异。采用Logistic回归分析CysC与认知功能障碍的相关性。【结果】两组SECF总分及其分项定向力、广度、动物名、计算、分类、临摹、言语、识记的评分差异均有统计学意义（P〈0．05）；在各项目异常病例数比率的比较中，总分、定向力、动物名、计算、临摹、言语的差异均有统计学意义（P〈0．05）；单因素Logistic回归分析发现，与低CysC组相比，高CysC组出现认知功能异常的0R值为2．538，但经多因素Logistic回归分析显示年龄、男性、尿酸是发生认知功能损害的独立危险因素，CysC未进入方程。【结论】血清CysC水平可能与老年2型糖尿病患者认知领域功能障碍相关，但并未独立相关。展开更多
基金National Natural Science Foundation of China,No.39770300,30070873the Overseas Chinese Affairs Office of the State Council Foundation,No.98-33
文摘AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (Deltapsim). METHODS: Cell culture, cell counting, ELISA assay, TUNEL, flow cytometry, Western blot and fluorometric assay were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanism. RESULTS: JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Caspases 8 and 9 were activated during apoptosis as judged by the appearance of cleavage products from procaspase and the caspase activities to cleave specific fluorogenic substrates. To elucidate whether the activation of caspases 8 and 9 was required for the apoptosis induction, we examined the effect of caspase-specific inhibitors on apoptosis. The results showed that caspase inhibitors significantly inhibited the apoptosis induced by JTE-522. In addition, the membrane translocation of Bax and cytosolic release of cytochrome C accompanying with the decrease of the uptake of Rhodamin 123, were detected at an early stage of apoptosis. Furthermore, Bax translocation, cytochrome C release, and caspase 9 activation were blocked by Z-VAD.fmk and Z-IETD-CHO. CONCLUSION: The present data indicate a crucial association between activation of caspases 8, 9, cytochrome C release, membrane translocation of Bax, loss of Deltapsim and JTE-522-induced apoptosis in AGS cells.
文摘【目的】探讨血清胱抑素C（cystatinC，CysC）与老年2型糖尿病患者认知功能障碍的关系。【方法】选取老年2型糖尿病患者10。例，根据CysC水平分为高CysC组、低CysC组，比较两组各l临床特征及老年认知功能量表（scale of elderly cognitive function，SECF）得分的差异。采用Logistic回归分析CysC与认知功能障碍的相关性。【结果】两组SECF总分及其分项定向力、广度、动物名、计算、分类、临摹、言语、识记的评分差异均有统计学意义（P〈0．05）；在各项目异常病例数比率的比较中，总分、定向力、动物名、计算、临摹、言语的差异均有统计学意义（P〈0．05）；单因素Logistic回归分析发现，与低CysC组相比，高CysC组出现认知功能异常的0R值为2．538，但经多因素Logistic回归分析显示年龄、男性、尿酸是发生认知功能损害的独立危险因素，CysC未进入方程。【结论】血清CysC水平可能与老年2型糖尿病患者认知领域功能障碍相关，但并未独立相关。
