Serratia fonticola and its role as a single pathogen causing emphysematous pyelonephritis in a non-diabetic patient:A case report

BACKGROUND Diagnosis of emphysematous pyelonephritis has been described around the world for some decades,frequently associated with Escherichia coli and other anaerobic,gas-forming bacteria and mostly in patients living with diabetes.We present a case report of emphysematous pyelonephritis in a non-diabetic patient caused by Serratia fonticola as well as a brief literature review to draw attention to this rare pathogen as a cause of pyelonephritis.CASE SUMMARY A 38-year-old female presented with fever,severe pain in the right flank and changes in urinary habits.She was admitted,and emphysematous pyelonephritis was confirmed by an abdominal computerized tomography and urine cultures;the latter showed Serratia fonticola as a single pathogen.After 3 d of being treated with piperacillin/tazobactam and percutaneous drainage she became afebrile,and the gas presence reduced.CONCLUSION Emphysematous pyelonephritis infections in non-diabetic patients are rare but can be severe and life-threatening.This case suggests that Serratia fonticola infection can occur in patients undergoing invasive or instrumented procedures.

一株产低温碱性磷脂酶A_1耐冷细菌的筛选及发酵条件的初步研究

从新疆天山一号冰川冻土中筛选到一株产低温碱性磷脂酶A1的菌株xjF1,初步鉴定为居泉沙雷氏菌(Serratia fonticola)。该菌属于耐冷菌,具有产两种酶的特性。其产生磷脂酶A1的最适碳源为木糖,最适氮源为磷酸氢二铵。在接种量为2%,250mL三角瓶装液量为50mL,25℃,250r/min摇床振荡培养36h时,磷脂酶A1活力最高达17.5U/mL。该磷脂酶A1的最适作用温度和pH值是35℃和9.0,在12℃仍具有69%的酶活力,但在60℃保温10min即丧失86%的活力,对热敏感。

一起由阴沟肠杆菌和居泉沙雷菌引起牛肺炎的诊断

乌鲁木齐市某牲畜交易市场有犊牛死亡,另有其他病例发生,患病犊牛主要表现为呼吸困难。采集病料进行实验室诊断,根据临床症状及细菌分离鉴定结果,判定为阴沟肠杆菌和居泉沙雷菌引起的牛肺炎,药敏试验显示,这两种菌均对亚胺培南、环丙沙星等药物敏感,经治疗后病牛恢复健康。本研究是牛感染阴沟肠杆菌和居泉沙雷菌引起肺炎并导致死亡的首次报道,为该病的诊断和防治提供参考。

一起居泉沙雷菌感染性腹泻的病原学检验

目的调查一起居泉沙雷菌的感染及病原学检验。方法对居泉沙雷菌感染性腹泻的患者进行流行病学调查,及细菌学检验。结果通过对患者粪便标本的病原学检验,检出居泉沙雷菌,并做药敏实验,指导临床用药。结论居泉沙雷菌广泛分布于自然界的水及植物中,但引起人类感染少,随着现在滥用抗生素,居泉沙雷菌抗药性也不断增加,应该引起足够的重视。

某市中心医院39株居泉沙雷菌的感染特征及耐药性分析

目的了解居泉沙雷菌的标本来源及耐药性,为临床合理用药提供参考。方法回顾性分析2010年1月—2016年12月笔者所在医院临床分离出的39株居泉沙雷菌标本来源及耐药性,采用VITEK-2 Compact全自动细菌鉴定药敏分析仪进行细菌鉴定及药敏试验,根据CLSI标准用WHONET 5.6分析菌株耐药率。结果 39株居泉沙雷菌标本来源以血液为主,共13株,占33.3%,其次为胆汁和尿液,分别占20.5%、17.9%;对居泉沙雷菌耐药率<10%的抗菌药物依次是阿米卡星、亚胺培南、厄他培南、哌拉西林/他唑巴坦、妥布霉素;10%~20%的依次是头孢替坦、头孢吡肟、呋喃妥因、庆大霉素;20%~30%的依次是环丙沙星、左旋氧氟沙星、头孢拉定;30%~40%的是复方新诺明、氨曲南、头孢曲松。结论居泉沙雷菌可引起血流、胆道、泌尿道感染,耐药性复杂,临床应根据药敏试验合理用药。