To evaluate the antitumor activity of Lactuca serriola against EAC （Ehrlich ascites carcinoma） in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at ...To evaluate the antitumor activity of Lactuca serriola against EAC （Ehrlich ascites carcinoma） in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at （100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw） against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters （e.g., hemoglobin content, red and white blood cells count and differential cell count） after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose （P 〈 0.05）. It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity.展开更多
The study was designed to investigate the anti-inflammatory and composition of essential oil of two plant family Asteraceae, Achillea fragrantissima and Lactuca serriola growing under dry desert condition. The anti-in...The study was designed to investigate the anti-inflammatory and composition of essential oil of two plant family Asteraceae, Achillea fragrantissima and Lactuca serriola growing under dry desert condition. The anti-inflammatory effect of volatile oil extracted by hydrodistillation of plants was studied using carrageenan induced paw edema. Essential oil （100 mg/kg） and （200 mg/kg） were tested the two plant show high inhibition after 4 h, concentration （200 mg/kg） show high inhibition than （100 mg/kg） after 4 h. Sesqui sabinene hydrate, Azuline and u-Bisabolol are the main constituents of the volatile oil were investigated by capillary GC （gas chromatography） and GC-MS （gas chromatography-mass spectrometry）. The discussion shows the role of chemical compound azulene in inflammatory inhibition.展开更多
为明确毒莴苣EST序列中SSR的特点,并开发EST-SSR分子标记用于毒莴苣及其近似种的分类鉴定及遗传多样性分析,以筛选的7 438条毒莴苣EST序列为基础,利用SSRIT软件结合人工搜索查找SSR位点,并采用Primer Premier 5.0软件设计EST-SSR引物,...为明确毒莴苣EST序列中SSR的特点,并开发EST-SSR分子标记用于毒莴苣及其近似种的分类鉴定及遗传多样性分析,以筛选的7 438条毒莴苣EST序列为基础,利用SSRIT软件结合人工搜索查找SSR位点,并采用Primer Premier 5.0软件设计EST-SSR引物,以毒莴苣及其近似种为材料对所合成引物进行多态性检测,应用UPGMA法构建11个毒莴苣及其近似种的系统发育树。结果表明,共搜索到SSR位点1 465个,发现重复基元598种,其中二、三、六核苷酸重复是主要的重复类型。合成的24对引物中有9对表现出多态性,利用这9对EST-SSR引物分析毒莴苣等11份材料的亲缘关系,其相似性系数在0.432~1.000之间;于0.513处可被划分为3大类：Ⅰ类包括翅果菊和北山莴苣,Ⅱ类只有宿根莴苣1种,Ⅲ类包括毒莴苣和莴苣。展开更多
文摘To evaluate the antitumor activity of Lactuca serriola against EAC （Ehrlich ascites carcinoma） in Swiss albino mice. The in vivo antitumor activity of the methanol extract of plant Lactuca serriola was evaluated at （100 mg/kg and 200 mg/kg of hole plant and 200 mg/kg, 400 mg/kg of fruit bw） against EAC using mean survival time. After administration of the extracts of Lactuca serriola, viable EAC cell count and body weight in the EAC tumor hosts were observed. The animal was also observed for improvement in the hematological parameters （e.g., hemoglobin content, red and white blood cells count and differential cell count） after treatment of plant extract. Intraperitoneal administration of plant extracts reduced viable EAC cells, increased the survival time and restored altered hematological parameters. Significant efficacy was observed for fruit extract at high concentration 400 mg/kg dose （P 〈 0.05）. It can be concluded that the methyl extract ofLactuca serriola possesses significant antitumor activity.
文摘The study was designed to investigate the anti-inflammatory and composition of essential oil of two plant family Asteraceae, Achillea fragrantissima and Lactuca serriola growing under dry desert condition. The anti-inflammatory effect of volatile oil extracted by hydrodistillation of plants was studied using carrageenan induced paw edema. Essential oil （100 mg/kg） and （200 mg/kg） were tested the two plant show high inhibition after 4 h, concentration （200 mg/kg） show high inhibition than （100 mg/kg） after 4 h. Sesqui sabinene hydrate, Azuline and u-Bisabolol are the main constituents of the volatile oil were investigated by capillary GC （gas chromatography） and GC-MS （gas chromatography-mass spectrometry）. The discussion shows the role of chemical compound azulene in inflammatory inhibition.
文摘为明确毒莴苣EST序列中SSR的特点,并开发EST-SSR分子标记用于毒莴苣及其近似种的分类鉴定及遗传多样性分析,以筛选的7 438条毒莴苣EST序列为基础,利用SSRIT软件结合人工搜索查找SSR位点,并采用Primer Premier 5.0软件设计EST-SSR引物,以毒莴苣及其近似种为材料对所合成引物进行多态性检测,应用UPGMA法构建11个毒莴苣及其近似种的系统发育树。结果表明,共搜索到SSR位点1 465个,发现重复基元598种,其中二、三、六核苷酸重复是主要的重复类型。合成的24对引物中有9对表现出多态性,利用这9对EST-SSR引物分析毒莴苣等11份材料的亲缘关系,其相似性系数在0.432~1.000之间;于0.513处可被划分为3大类：Ⅰ类包括翅果菊和北山莴苣,Ⅱ类只有宿根莴苣1种,Ⅲ类包括毒莴苣和莴苣。
