Correlation of Serum C-Reactive Protein with Disease Severity in Tuberculosis Patients

Purpose: To study the factors influencing sputum smear conversion including Serum C-Reactive Protein (CRP) and its correlation with disease severity in tuberculosis patients. Method: Levels of Serum-CRP concentrations were deter-mined in 60 patients with pulmonary tuberculosis, 30 healthy volunteers and patients in follow-up after completion of antitubercular treatment (DOTS therapy). Results: Serum-CRP levels were found to be significantly higher in smear-positive group as compared with the follow-up patients and smear-negative control group. The values were 43.65 ± 23.68, 9.88 ± 5.23 and 4.04 ± 3.85 mg/L respectively (P Conclusion: Serum-CRP levels are significantly correlated with disease severity in patients with active pulmonary tuberculosis. Thus these findings from the present study would certainly add new criteria for early diagnosis of TB, which may lead to development of new strategies to treat TB.

Comparative evaluation of acute phase proteins by C-reactive protein(CRP)and serum amyloid A(SAA)in nonhuman primates and feline carnivores

The feasibility of a commercially available assay for C-reactive protein(CRP,CRP for humans:hCRP,and CRP for dogs:vCRP)and a trial reagent of serum amyloid A(SAA,vSAA for animals)were applied to the measurement of acute phase proteins in zoo animals,particularly in nonhuman primates and feline carnivores was evaluate.Results showed that hCRP and vSAA methods were applicable to measure CRP and SAA in Haplorhini.There was a highly signifcant correlation between both parameters with remarkably high correlation coefcient.A higher proportion of Bonnet macaques in Haplorhini,and the linear regression with good correlation between hCRP and vSAA levels were observed.Reference values in healthy Bonnet macaques were hCRP(46.86±30.97 nmol/L)and vSAA(9.06±1.95μg/mL).Although Ring-tailed lemur,which belonging to Strepsirrhini,showed low vSAA concentrations(reference values:1.08±0.47μg/mL),vSAA in patients was apparently elevated.The vCRP and vSAA methods were applicable to measurements of CRP and SAA in feline carnivores for highly signifcant correlation between both parameters.Theses two methods were also been deteded in lions,tigers and cheetahs.vSAA assays can be applied to measure SAA levels in other carnivores and herbivores.In conclusion,vSAA systems have potential utility as diagnostic tools for health screening and prediction in zoo animals.

APPLICATION OF NONIONIC TEMPERATURE SENSITIVE HYDROGEL FOR CONCENTRATION OF PROTEIN AQUEOUS SOLUTION

Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide (PNIPA) and poly N-n-propyl-acrylamide (PNNPA) temperature sensitive hydrogels were chosen as the nonionic temperature sensitive hydrogels for concentration of very dilute aqueous protein solution. The separation properties of PNIPA and PNNPA hydr0gels with different network dimensions were studied and the modification of the hydrogels was surveyed in order to decrease their surface adsorption of protein molecules. The experimental results of the concentration of BSA (Bovin serum albumin) dilute aqueous solution by hydroxylpropyl methacrylate (HPMA) copolymerized PNIPA hydrogel were given. The value and the limitation of concentration of dilute aqueous protein solution by this method was evaluated.

Effects of Crude Protein Level of Concentrate Supplement on Production Performance and Serum Biochemical Parameters in Post-fattening Hainan Yellow Cattle

[Objective]The paper was to investigate the effects of crude protein level of concentrate supplement on production performance and serum biochemical parameters of post-fattening Hainan Yellow Cattle.[Method]Twelve Hainan Yellow Cattles with the same genetic backgrounds and similar initial weight of(168.13±0.55)kg were randomly divided into three treatments.Cattle were fed with concentrate supplement containing different levels of crude protein.The trial lasted 60 d.[Result]The daily gain of 17.56%crude protein group was significantly higher than those of 15.55%and 13.56%crude protein groups(P<0.05),and the gross profit of 17.56%crude protein group was markedly higher than that of 13.56%crude protein group(P<0.05),but the feed gain ratio of 17.56%crude protein group was significantly lower than that of 13.56%crude protein group(P<0.05).Compared with 15.55%and 13.56%crude protein groups,the serum total protein content markedly increased(P<0.05),but the urea nitrogen level significantly decreased(P<0.05).[Conclusion]When crude protein level of concentrate supplement was 17.56%,post-fattening Hainan Yellow Cattle could obtain better production performance.

低蛋白精料补充料对泌乳期放牧牦牛体增重、血清生化指标及乳成分的影响

试验旨在研究低蛋白精料补充料补充两种主要过瘤胃氨基酸对泌乳期放牧牦牛体增重、血清生化指标及乳成分的影响。选取健康状况良好、体重相近的24头泌乳牦牛作为试验动物,随机分为3组,每组8个重复。高蛋白组(HP组)饲喂粗蛋白水平为16.66%的精料补充料,中蛋白组(MP组)饲喂粗蛋白水平为15.05%的精料补充料并额外添加0.23%过瘤胃赖氨酸和0.03%过瘤胃蛋氨酸、低蛋白组(LP组)饲喂粗蛋白水平为13.57%的精料补充料并额外添加0.46%过瘤胃赖氨酸和0.07%过瘤胃蛋氨酸。预试期8 d,正试期40 d。结果表明:3组母牦牛终末体重及净增重指标无显著差异(P>0.05);LP组血清中葡萄糖的含量显著低于其他两组(P<0.05),HP组血清尿素氮的含量显著高于LP组(P<0.05),各组间血清其他指标差异均不显著(P>0.05);3组泌乳期牦牛产奶量无显著差异(P>0.05),LP组乳脂率显著高于MP组和HP组(P<0.05),HP组总固形物含量显著低于MP组和LP组(P<0.05)。综上得出,降低泌乳期牦牛精料补充料的粗蛋白水平并补充相应的过瘤胃氨基酸不影响泌乳期牦牛的生产性能,且在降低饲料中豆粕用量的同时能改善机体蛋白质的利用情况,提高了饲粮的氮利用率。

Autologous peripheral blood-derived orthobiologics:Different types and their effectiveness in managing knee osteoarthritis

Knees are the most commonly impacted weight-bearing joints in osteoarthritis(OA),affecting millions of people worldwide.With increasing life spans and obesity rates,the incidence of knee OA will further increase,leading to a significant increase in the economic burden.Conventional treatment modalities utilized to manage knee OA have limitations.Over the last decade,the role of various autologous peripheral blood-derived orthobiologics(APBOs)for the treatment of knee OA has been extensively investigated.This editorial provided an overview and focused on defining and shedding light on the current state of evidence based on the most recent published clinical studies concerning the use of APBO for the management of knee OA.While numerous studies have demonstrated promising results for these preparations,a notable gap exists in the comparative analysis of these diverse formulations.This absence of head-to-head studies poses a considerable challenge for physicians/surgeons in determining the optimal preparation for managing knee OA and achieving sustained longterm results.Thus,more adequately powered,multicenter,prospective,doubleblind,randomized controlled trials with longer follow-ups are needed to establish the long-term efficacy and to aid physicians/surgeons in determining the optimal APBO for the management of knee OA.

Interleukins and Acute Phase Proteins of Bovine Sera during Natural Helminth Burden in Ibadan Nigeria

Inflammatory reactions in the gastrointestinal tract play an important role in the pathogenesis of gastroenteritis in bovine helminthosis. This study determined the serum concentrations of cytokines induced acute phase proteins (Serum Amyloid A (SAA), Haptoglobin (Hp) and C-reactive protein (CRP)) and the levels of immunoreactive interleukins (IL-1, IL-2, IL-3, IL-4, IL-5 and IL-6) in cattle naturally infected with helminths. We sampled a total of 480 slaughtered cattle of both sexes in a major abattoir in Ibadan, Nigeria. Sedimentation, floatation and modified McMaster techniques were employed to determine the degrees of helminth infections. Animals with eggs per gram (epg) less than 200 were adjudged to be apparently healthy. The serum concentrations of interleukins and acute phase proteins were determined using the Technicon AutoAnalyzer Model AA II. It was found that mean SAA (μg/l) and CRP (%) levels were significantly (P 0.05). Therefore, some acute phase proteins are involved in the pathophysiology of bovine helminthosis and are closely related to the inflammatory activation of the disease. In lieu of these findings, it is suggested that systemic markers of inflammation can identify subjects at high risk of natural bovine helminthosis and that IL-6 and SAA may be used as indicators for bovine helminthosis.

Changes of serum CRP, serum uric acid and immunoglobulin in patients with renal damage of systemic lupus erythematosus

Objective: To observe the expression level of serum CRP, serum uric acid and immunoglobulin (IgG, IgA and IgM) in patients with renal damage of systemic lupus erythematosus, and explore their clinical application value. Methods: A total of 95 patients with SLE were divided into lupus nephritis group (LN group, n = 64) and non-lupus nephritis group (NLN group n=31) according to the diagnostic criteria of lupus nephritis, and another 35 healthy subjects were selected as normal control group. Blood samples were collected from all subjects before treatment. Serum CRP and serum uric acid concentrations were measured by ELISA. The levels of serum immunoglobulins were detected by immunoturbidimetry in the same time. Finally, the results were analyzed statistically. Results: The levels of serum CRP and serum uric acid in patients with SLE were significantly higher than those in control group. The levels of serum CRP and serum uric acid in LN group of SLE patients were significantly higher than those in NLN group and control group. Serum CRP level in NLN group was significantly higher than that in control group. Serum uric acid level in NLN group was slightly higher than that of the control group, but the difference was not statistically significant. The level of serum immunoglobulin in SLE patients was higher than that in control group. The levels of serum immunoglobulin IgG, IgA and IgM in LN group of SLE patients were significantly higher than those in NLN group and control group. The levels of serum immunoglobulin IgG, IgA and IgM in NLN group of SLE patients were all higher than those in control group. Conclusion: Humoral immune of SLE patients with renal injury was activated at different degree, B-cell hyperthyroidism, severe systemic inflammatory response and kidney damage at varying degrees. Serum CRP, serum uric acid and immunoglobulin (IgG, IgA and IgM) may be involved in SLE renal injury pathological process, which is the early diagnosis of reference indicators.

Serum Markers of Systemic Inflammation in Patients with Pulmonary Tuberculosis

Serum markers of systemic inflammation （SAA （serum amyloid protein A）, CRP （C-reactive protein）, cq-AT （ct l-antitripsin）, Hp （haptoglobin） and F （fibrinogen）） have been studied in 317 patients with pulmonary tuberculosis. It was established that sensitivity of SAA as an activity marker was higher than of other acute phase reactants, because its levels were increased in 98.8% patients, whereas the level of CRP exceeded the norm in 80.8%, α1-AT--in 59.3%, Hp----in 43.5% and F--in 63.1% cases. The degree of increase of acute phase reactants directly related to the expressiveness of tuberculosis intoxication, the extent of the process in the lung and the quantity of MTB （M. tuberculosis） in sputum. We concluded that SAA was a useful marker of the process activity in patients with pulmonary tuberculosis and its sensitivity was higher than that of other acute phase reactants.

The effects of enhanced external counterpulsation on the serum level of C-reactive protein and endothelin-1 in patients with ischemic stroke

Objective To explore the effects of enhanced external counterpulsation(EECP)on the serum level of C-reactive protein and endothelin-1 in patients with cerebral ischemic stroke,to provide clinical evidence for the treatment and secondary prevention of patients with serebral ischemic stroke.Methods Total 187 patients with ischemic stroke and enrolled measure the serum level

高精料饲粮中添加烟酸对绵羊瘤胃发酵参数、微生物蛋白浓度和血清生化指标的影响

本试验旨在研究高精料饲粮中添加烟酸对绵羊瘤胃发酵参数、微生物蛋白浓度和血清生化指标的影响。选取8月龄左右、体重[(36.04±1.81) kg]相近且健康的哈萨克公羊24只,试验前安装好永久性瘤胃瘘管,随机分为4组,每组6只。对照组饲喂精粗比为70∶30的基础饲粮,试验Ⅰ、Ⅱ、Ⅲ组分别在饲喂基础饲粮的基础上补喂100、130和160 mg/d烟酸。试验期共18 d,其中预试期14 d,正试期4 d。结果显示:1)与对照组相比,饲喂后1、3、5、7 h试验Ⅱ组瘤胃液pH极显著提高(P<0.01);试验Ⅱ组瘤胃液氨态氮浓度在饲喂后1、3、5 h显著或极显著高于对照组(P<0.05或P<0.01);试验Ⅱ组瘤胃液丙酸浓度在饲喂后3 h极显著高于对照组及试验Ⅰ、Ⅲ组(P<0.01),在饲喂后5 h显著高于对照组和试验Ⅰ组(P<0.05)。2)试验Ⅱ组瘤胃液微生物蛋白浓度在饲喂前0 h以及饲喂后1、3、5、7 h均显著或极显著高于对照组(P<0.05或P<0.01)。3)试验Ⅱ组血清葡萄糖含量显著高于对照组和试验Ⅰ组(P<0.05)。综上所述,在精粗比为70∶30的高精料饲粮中添加130 mg/d烟酸提高了绵羊瘤胃液pH及氨态氮、丙酸及微生物蛋白浓度,同时提高了血清中葡萄糖含量。

血清肝细胞生长因子评估儿童肺炎支原体肺炎的临床价值

目的探讨在儿童肺炎支原体肺炎评估中实施血清肝细胞生长因子水平检测的临床价值。方法2019年1月—2021年6月从茂名市人民医院择取60例肺炎支原体肺炎患儿作为研究组,根据病情严重程度可以分为两组,20例重症支原体肺炎患儿纳入研究一组,40例非重症支原体肺炎患儿纳入研究二组,另外择取20例健康体检儿童作为对照组,通过酶联免疫吸附法对血清肝细胞生长因子水平进行检测,并通过全自动生化分析仪测定研究组患儿的血清C-反应蛋白浓度、乳酸脱氢酶浓度,对照分析上述几组的检验结果。结果研究组血清肝细胞生长因子水平均高于对照组,差异有统计学意义(P<0.05);从临床特征与实验室特点分析,研究一组年龄大于研究二组,住院时间比研究二组长,支气管肺炎类型的胸片患者多于研究二组,差异有统计学意义(P<0.05);研究一组的血清肝细胞生长因子、血清C-反应蛋白浓度、乳酸脱氢酶浓度明显高于研究二组,差异有统计学意义(P<0.05)。结论在儿童肺炎支原体肺炎评估中,血清肝细胞生长因子水平检测具备显著临床价值,可以评估病情严重程度,还可以为临床治疗效果评估提供指导作用。

基于共振瑞利散射血清蛋白浓度检测的研究

血清中蛋白质浓度在临床诊断中有重要意义,而目前广泛使用的紫外-可见分光光度计、荧光分光光度计等仪器结构复杂、昂贵、体积庞大、耗电量高等因素,都无法满足现场高精度检测的要求。设计了基于四羧基酞菁锌-蛋白质体系的共振瑞利散射光谱检测系统,系统以405nm宽禁带半导体激光器为激励光源,以475nm窄带带通滤光片为单色器,以蓝光增强光敏二极管的低噪声高增益光电放大器为探测器。通过实验可知,该溶液强吸收波长为420nm附近,在该激励光作用下,其共振波长处会产生共振瑞利散射,散射强度与蛋白质的含量成比例,可以利用四羧基酞菁锌为光谱探针的共振散射法来测定血清蛋白,其线性检出范围为10～50mg.mL-1,检出限为0.001mg.mL-1。新开发的血清蛋白质测试装置具有体积小、成本低、功耗小、使用方便等优点。

精料补充料蛋白质水平对育肥前期海南黄牛生长性能及血清生化指标的影响

为研究精料补充料蛋白质水平对育肥前期海南黄牛生长性能及血清生化指标的影响,选取12头遗传背景一致、初始体重相近[(137.08±0.29) kg]海南黄牛公牛12头,随机分为3个处理组,分别饲喂不同粗蛋白质水平的精料补充料。试验为期60 d。结果表明:19.56%粗蛋白质组日增重显著高于15.55%和17.56%粗蛋白质组(P<0.05),而料重比则显著低于15.55%和17.56%粗蛋白质组(P<0.05)。19.56%粗蛋白质组饲料成本显著低于15.55%和17.56%粗蛋白质组(P<0.05),而毛利润显著高于15.55%和17.56%粗蛋白质组(P<0.05)。19.56%粗蛋白质组血清总蛋白、球蛋白、葡萄糖含量较高,而尿素氮水平较低。综合认为,粗蛋白质水平为19.56%的精料补充料可使育肥前期海南黄牛获得较好的生长性能。

不同蛋白质水平的氨基酸平衡日粮对泌乳母猪生产性能、血清指标和乳汁氨基酸含量的影响

本试验旨在探讨理想氨基酸模式下,不同粗蛋白质水平的日粮对泌乳母猪生产性能、血清指标和乳汁氨基酸含量的影响.选用3-6胎次、体况相似、预产期相近的60头长大二元杂交母猪,随机分成4个组,每组15头母猪,分别饲喂粗蛋白质18%(对照组)和粗蛋白质17%(试验1组)、16%(试验2组)、15%(试验3组)低蛋白氨基酸平衡的日粮,试验期从妊娠110天到哺乳期21天结束.结果表明:日粮蛋白质水平对母猪泌乳期日均采食量、体重损失、断奶后发情间隔、仔猪哺乳期窝增重和平均日增重的影响不显著(P>0.05);母猪血清和乳汁游离氨基酸含量处理组间差异均不显著(P>0.05);15%蛋白组和16%蛋白组血清尿素氮含量显著低于18%蛋白组(P<0.05).

腹水浓缩回输腹腔前后血清蛋白及RAAS系统激素水平变化研究

目的:探讨腹水超滤浓缩回输对肝硬化顽固性腹水、肝癌腹水及其他腹腔恶性腹水患者的血浆白蛋白和RAAS系统激素水平的影响。方法:选取2014年3月-2016年2月本院符合标准的230例腹水患者为研究对象,将其随机分为两组。治疗组120患者进行腹水超滤浓缩回输治疗,对照组110患者进行常规放腹水、补充白蛋白治疗。检测治疗前后两组患者的腹水症状、血浆白蛋白和RAAS水平并进行比较。结果:与治疗前比较,两组患者治疗后血清总蛋白及清蛋白水平均有所升高,治疗组升高更加明显,差异均有统计学意义(P<0.05)。治疗后患者RAAS系统激素水平均呈不同程度下降,其中治疗组肾素、血管紧张素Ⅱ及醛固酮水平较治疗前均下降(P<0.05),对照组仅血管紧张素Ⅱ及醛固酮较治疗前下降(P<0.05);治疗后两组患者的RAAS激素水平比较,差异均有统计学意义(P<0.05)。治疗后,治疗组患者体重下降、腹围减少、24 h尿量增加更加明显,总有效率为83.33%,与对照组比较差异均有统计学意义(P<0.05)。结论:腹水超滤浓缩回输治疗肝硬化顽固性腹水、肝癌腹水及其他腹腔恶性腹水效果较好,可能与其升高白蛋白和降低RAAS系统激素水平作用有关。

牛血清白蛋白与叶酸相互作用的荧光光谱法在蛋白质含量测定中的应用

研究了不同质量浓度蛋白质与同质量浓度叶酸相互作用的荧光光谱和产生的荧光强度变化.结果表明,随蛋白质质量浓度的增加产生的荧光强度也逐渐增加,且蛋白质质量浓度与荧光强度之间具有良好的线性关系.还研究了三聚氰氨对蛋白质质量浓度测量的影响,以及用此方法测奶粉中蛋白质含量的可行性.据此建立了有效测量蛋白质质量浓度的以荧光光谱为基础的新方法.该法最低检测限为5×10-6mg.L-1,检测范围广,线性范围是3.77×10-2~827 mg.L-1（827 mg.L-1不是最大范围,最大值未测定）,相关系数为0.998 9,加标回收率为93.71%~106.2%.

不同方法提取乳清浓缩蛋白的研究

[目的]探索一条经济、简单易行的乳清浓缩蛋白提取工艺。[方法]以乳清废液为原料,分别采用醇沉法、碱沉法、醇+碱沉法提取其中的乳清浓缩蛋白,比较不同方法的提取效果。[结果]影响醇沉法提取乳清蛋白产量的因素依次为:沉淀时间>乙醇浓度>沉淀温度,最佳醇提条件为:乙醇浓度100%,沉淀温度50℃,沉淀时间1.5 h;影响碱沉法提取乳清蛋白产量的因素依次为:沉淀时间>pH值>沉淀温度,最佳碱提条件为:pH值10,沉淀温度30℃,沉淀时间1.5 h;影响醇+碱沉法提取乳清蛋白产量的因素依次为:沉淀时间>沉淀温度>pH值>乙醇浓度,最佳提取条件为:乙醇浓度90%,沉淀时间2.0 h,pH值7,沉淀温度20℃。[结论]醇+碱沉法提取乳清浓缩蛋白的效果最佳。

由大豆浓缩蛋白(SPC)乳清制备高纯度异黄酮苷元的研究

以价格低廉、易污染环境的大豆浓缩蛋白(SPC)乳清为原料,采用等电点结合盐析法沉淀蛋白质、活性炭脱色、离子交换树脂脱盐、HPD-100型非极性大孔树脂吸附纯化、盐酸水解等工艺,制备高纯度的大豆异黄酮苷元产品。经高效液相色谱(HPLC)等方法分析,大豆异黄酮的纯度为(58.4±2.5)%(w/w),总回收率为(78.3±2.6)%;异黄酮苷元的相对含量≥95%(w/w),其中染料木素(Genistein)与大豆甙元(Daid-zein)的含量分别为(23.4±0.8)%和(31.2±0.9)%,而黄豆黄素(G lycitein)的含量极低。产品纯度和附加值高,既能克服当前异黄酮提取工艺中原料成本高、预处理方法繁琐、产品纯度低等缺点,又能实现废物利用,具有良好的推广应用与产业化前景。

基于亲和素-生物素双夹心体系测定外源性蛋白血清动态浓度的非抗体依赖新方法

建立一种非抗体依赖的检测外源性蛋白多肽分子代谢动力学血清浓度及动态变化的新方法.链亲和素为捕获分子,加入待测生物素标记蛋白或合成肽,辣根过氧化物酶标记链亲和素为检测分子,构成链亲和素-生物素标记大分子-酶链亲和素的双夹心体系,并进行特异性、敏感性、准确性及稳定性评价.本检测体系灵敏度高,可达0.3125μg/L,且可通过改变亲和素包被浓度调整检出敏感度与检测范围.准确性回收率为97.82%～107.92%,批内、批间变异系数分别<5.76%和<8.42%,并成功应用在生物素标记人血清白蛋白(biotin-HSA)与生物素标记鸡卵黏蛋白(biotin-OVM)的小鼠血清浓度动态检测.本方法不依赖抗体与放射性核素,可望在外源性蛋白多肽大分子及其药物的体内定量检测和代谢动力学研究中广泛应用.