Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control...Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control,sham operation, model and EA groups, and each of them were further divided into IR 12 hr, IR 24 hr and IR 48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL 1β and tumor necrosis factor (TNF) α contents were assayed by using radioimmunoassay. Results: Results showed that serum IL 1β and TNF α contents of model group were significantly higher than those of normal control and sham operation groups at IR 12 hr, 24 hr and 48 hr (P<0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL 1β and TNF α levels of EA group were strikingly lower than those of model group (P<0.01), suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL 1β and TNF α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.展开更多
[ Objective] To study the effects of astragalus powder on serum lipids and body fat contents of fast large-scale quality chickens, and thus provide a theoretical basis for its application in poultry production,[ Metho...[ Objective] To study the effects of astragalus powder on serum lipids and body fat contents of fast large-scale quality chickens, and thus provide a theoretical basis for its application in poultry production,[ Method ] All the 180 1-day-old healthy Liangfeng chickens were randomly divided into six groups. Group 1 was the control group, fed with basal diet; group 2, group 3 and group 4 were fed with basal diet added with 0.75%, 1.0% and 1.25% astragalus powder, respectively; group 5 and group 6 were separately fed with basal diet added with 1.0% and 1.25% astragalus pow- der, but the astragalus powder was used for 10 d and then was forbidden for 10 d in turn during the whole experiment. The content of triglyceride （TG）, total cholesterol （TCHO）, high-density lipoprotein-Cholesterol （ HDL-C）, and low-density lipoprotein-Cholesterol （LDL-C） were respectively determined when the chickens were 35-day-old and 63-day-old, while the percentage of abdominal fat （PAF） as well as intramuscular fat （IMF） and subcutaneous fat ply （SFP） was measured when the chickens were 63-day-old. [ Result] In 35-day-old chickens, the levels of TCHO were sig- nificantly or very significantly lower in group 3, group 4, and group 6 than in control group （ P 〈0.05 or 0.01 ） ; the levels of LDL-C significantly lower in group 3 and group 6 （ P〈0. 05） ; the level of HDL-C significantly higher in group 5 （P〈0.05）. In 63-day-old chickens, the levels of TCHO and LDL-C were significantly or very significantly lower in group 3, group 4, and group 5 than in control group （ P 〈 0.05 or 0.01 ） while the levels of HDL-C were significantly higher in these groups; the PAF was significantly lower in group 3 （ P 〈 0.05）. [ Condasion] Astragalus powder should re- duce the levels of TG, TCHO and LDL-C, promote HDL-C content, and also play a certain regulative role in deposition of abdominal fat.展开更多
Wistar rats were exposed to trinitrotoluene (TNT) for 6 weeks. After initiation of TNT exposure, serum phosphorylase A activities and calcium contents were assayed for every 2 weeks. Both of these 2 parameters increas...Wistar rats were exposed to trinitrotoluene (TNT) for 6 weeks. After initiation of TNT exposure, serum phosphorylase A activities and calcium contents were assayed for every 2 weeks. Both of these 2 parameters increased in rats treated with 50 and 100 mg TNT/kg b.w. at 3 intervals. Serum phosphorylase A activities and calcium contents of TNT exposure worker increased too.展开更多
In this study, the therapeutic effect of acupuncture and its effect on serum tumor necrosis factor apha (TNF-α) level was observed in 25 cases of allergic bronchial asthma patients. Acupoints used were Dazhui (GV 14)...In this study, the therapeutic effect of acupuncture and its effect on serum tumor necrosis factor apha (TNF-α) level was observed in 25 cases of allergic bronchial asthma patients. Acupoints used were Dazhui (GV 14), Feishu (BL 13), Dingchuan (EX-B 1), Pishu (BL 20), Tanzhong (CV 17), Shenshu (BL 23) and Fengchi (GB 20), supplemented with other acupoints according to syndrome differentiation. After 15 sessions of treatment, results showed that the total effective rate was 96% . Before treatment, serum TNF-oc activity was significantly higher than that of healthy subjects (25 cases, P<0.01). After treatment, TNF-α level reduced considerably in comparison with that of pre-treatment (P < 0. 05). These findings indicate that acupuncture can significantly improve allergic asthma patients' clinical symptoms and lower serum TNF-α activity.展开更多
Patients with AIDS(Acquired Immunodeficiency Srudrome) have immunoldgic abnormalities resulting from functional defects in HIV(Human Immunodeficiency Virus)-infected CD4+lymphocytes and monocytes. Tumor necrosis facto...Patients with AIDS(Acquired Immunodeficiency Srudrome) have immunoldgic abnormalities resulting from functional defects in HIV(Human Immunodeficiency Virus)-infected CD4+lymphocytes and monocytes. Tumor necrosis factor-alpha(TNF-α)is known to mediate cachexcia.Todetermine if TNF is responsible for 'wasting syndrome' in AIDS.we studied serum levels and in vitro production of TNF-alpha in 21 HIV-seropositive men(10 AIDS,2 ARC, 9 HIV+)and 8 HIV seronreative controls.Both a bloassay and TNF-specific ELISA were used.All patiend except onebad undctectable levels of serum TNF. while control sera contained 4.2±2.5μg/L of TNF,Peripheral blood mononuclear cell(PBMNC)was tested for spontaneous,LPS-induced or PHA and PMA-induced TNF production at different times in culture(24-72h).Patients cells Produced significantlyless TNF than normal PBMNC under all experimental conditions:e.g,18-45μg/L in conttols vs.0.4～13μg/L in patients(P<0.001)at 72 hours using PHA and PMA as activators.Cell viabilitywas 85% in all cultures.No significant differences in TNF release were detected between AIDS patients with cachexia or opportunistic infectious and other HIV-seropositive men.This study showedthat most HIV-infected persons had no dctectable serum TNF-αand that their PBMNC produced subnormal quantities of this cytoklne in vitro,suggesting that TNF is not involved in the pathogenesis ofthe cacbexia associated with AIDS.展开更多
[Objectives]To establish a UPLC-UV method for the determination of polydatin in the serum of Wistar rats.[Methods]Acquity UPLC BEH shield RP18 column(1.7μm,2.1 mm×100 mm,Waters Corporation,USA)was used as the an...[Objectives]To establish a UPLC-UV method for the determination of polydatin in the serum of Wistar rats.[Methods]Acquity UPLC BEH shield RP18 column(1.7μm,2.1 mm×100 mm,Waters Corporation,USA)was used as the analytical column,acetonitrile-water(55∶45)was used as the mobile phase,the flow rate was 0.5 mL/min,and the column temperature was 30℃and the detection wavelength was 306 nm.[Results]The linear range of the established serum sample analysis method was 1.0-20.0μg/mL,and the correlation coefficient was r=0.9994;the intraday and interday RSD of Wistar rat serum was less than 3.0%,and the accuracy was higher than 90%.[Conclusions]This method is sensitive,accurate,and rapid.It is suitable for monitoring the concentration of polydatin in serum after intragastric administration,and can also be used for pharmacokinetics and bioavailability studies.展开更多
文摘Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control,sham operation, model and EA groups, and each of them were further divided into IR 12 hr, IR 24 hr and IR 48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL 1β and tumor necrosis factor (TNF) α contents were assayed by using radioimmunoassay. Results: Results showed that serum IL 1β and TNF α contents of model group were significantly higher than those of normal control and sham operation groups at IR 12 hr, 24 hr and 48 hr (P<0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL 1β and TNF α levels of EA group were strikingly lower than those of model group (P<0.01), suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL 1β and TNF α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.
文摘[ Objective] To study the effects of astragalus powder on serum lipids and body fat contents of fast large-scale quality chickens, and thus provide a theoretical basis for its application in poultry production,[ Method ] All the 180 1-day-old healthy Liangfeng chickens were randomly divided into six groups. Group 1 was the control group, fed with basal diet; group 2, group 3 and group 4 were fed with basal diet added with 0.75%, 1.0% and 1.25% astragalus powder, respectively; group 5 and group 6 were separately fed with basal diet added with 1.0% and 1.25% astragalus pow- der, but the astragalus powder was used for 10 d and then was forbidden for 10 d in turn during the whole experiment. The content of triglyceride （TG）, total cholesterol （TCHO）, high-density lipoprotein-Cholesterol （ HDL-C）, and low-density lipoprotein-Cholesterol （LDL-C） were respectively determined when the chickens were 35-day-old and 63-day-old, while the percentage of abdominal fat （PAF） as well as intramuscular fat （IMF） and subcutaneous fat ply （SFP） was measured when the chickens were 63-day-old. [ Result] In 35-day-old chickens, the levels of TCHO were sig- nificantly or very significantly lower in group 3, group 4, and group 6 than in control group （ P 〈0.05 or 0.01 ） ; the levels of LDL-C significantly lower in group 3 and group 6 （ P〈0. 05） ; the level of HDL-C significantly higher in group 5 （P〈0.05）. In 63-day-old chickens, the levels of TCHO and LDL-C were significantly or very significantly lower in group 3, group 4, and group 5 than in control group （ P 〈 0.05 or 0.01 ） while the levels of HDL-C were significantly higher in these groups; the PAF was significantly lower in group 3 （ P 〈 0.05）. [ Condasion] Astragalus powder should re- duce the levels of TG, TCHO and LDL-C, promote HDL-C content, and also play a certain regulative role in deposition of abdominal fat.
文摘Wistar rats were exposed to trinitrotoluene (TNT) for 6 weeks. After initiation of TNT exposure, serum phosphorylase A activities and calcium contents were assayed for every 2 weeks. Both of these 2 parameters increased in rats treated with 50 and 100 mg TNT/kg b.w. at 3 intervals. Serum phosphorylase A activities and calcium contents of TNT exposure worker increased too.
文摘In this study, the therapeutic effect of acupuncture and its effect on serum tumor necrosis factor apha (TNF-α) level was observed in 25 cases of allergic bronchial asthma patients. Acupoints used were Dazhui (GV 14), Feishu (BL 13), Dingchuan (EX-B 1), Pishu (BL 20), Tanzhong (CV 17), Shenshu (BL 23) and Fengchi (GB 20), supplemented with other acupoints according to syndrome differentiation. After 15 sessions of treatment, results showed that the total effective rate was 96% . Before treatment, serum TNF-oc activity was significantly higher than that of healthy subjects (25 cases, P<0.01). After treatment, TNF-α level reduced considerably in comparison with that of pre-treatment (P < 0. 05). These findings indicate that acupuncture can significantly improve allergic asthma patients' clinical symptoms and lower serum TNF-α activity.
文摘Patients with AIDS(Acquired Immunodeficiency Srudrome) have immunoldgic abnormalities resulting from functional defects in HIV(Human Immunodeficiency Virus)-infected CD4+lymphocytes and monocytes. Tumor necrosis factor-alpha(TNF-α)is known to mediate cachexcia.Todetermine if TNF is responsible for 'wasting syndrome' in AIDS.we studied serum levels and in vitro production of TNF-alpha in 21 HIV-seropositive men(10 AIDS,2 ARC, 9 HIV+)and 8 HIV seronreative controls.Both a bloassay and TNF-specific ELISA were used.All patiend except onebad undctectable levels of serum TNF. while control sera contained 4.2±2.5μg/L of TNF,Peripheral blood mononuclear cell(PBMNC)was tested for spontaneous,LPS-induced or PHA and PMA-induced TNF production at different times in culture(24-72h).Patients cells Produced significantlyless TNF than normal PBMNC under all experimental conditions:e.g,18-45μg/L in conttols vs.0.4～13μg/L in patients(P<0.001)at 72 hours using PHA and PMA as activators.Cell viabilitywas 85% in all cultures.No significant differences in TNF release were detected between AIDS patients with cachexia or opportunistic infectious and other HIV-seropositive men.This study showedthat most HIV-infected persons had no dctectable serum TNF-αand that their PBMNC produced subnormal quantities of this cytoklne in vitro,suggesting that TNF is not involved in the pathogenesis ofthe cacbexia associated with AIDS.
基金Project of Traditional Chinese Medicine Administration of Guangxi Zhuang Autonomous Region(GZZC2019147)Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2020(2020KY13034)+2 种基金The First Batch High-level Talent Scientific Research Project of The Affiliated Hospital of Youjiang Medical University for Nationalities in 2019(Y20196311)National Traditional Chinese Medicine Characteristic Technology Heritage Talent Training Program(2015481601003)Program of Youjiang Medical University for Nationalities(yy2018ky018).
文摘[Objectives]To establish a UPLC-UV method for the determination of polydatin in the serum of Wistar rats.[Methods]Acquity UPLC BEH shield RP18 column(1.7μm,2.1 mm×100 mm,Waters Corporation,USA)was used as the analytical column,acetonitrile-water(55∶45)was used as the mobile phase,the flow rate was 0.5 mL/min,and the column temperature was 30℃and the detection wavelength was 306 nm.[Results]The linear range of the established serum sample analysis method was 1.0-20.0μg/mL,and the correlation coefficient was r=0.9994;the intraday and interday RSD of Wistar rat serum was less than 3.0%,and the accuracy was higher than 90%.[Conclusions]This method is sensitive,accurate,and rapid.It is suitable for monitoring the concentration of polydatin in serum after intragastric administration,and can also be used for pharmacokinetics and bioavailability studies.
