Objective: To investigate the correlation of serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content with malignant proliferation of cancer cells in patients with gastric cancer. Methods:Superficial gastritis group (n=247...Objective: To investigate the correlation of serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content with malignant proliferation of cancer cells in patients with gastric cancer. Methods:Superficial gastritis group (n=247), gastric ulcer group (n=159) and gastric cancer group (n=97) who were pathologically diagnosed by gastroscopy in Chongqing wanzhou district people's hospital of 5 between August 2016 and August 2017 were selected, and the differences in serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content as well as proliferation and apoptosis gene expression in lesion tissue were compared among the three groups. Pearson test was used to evaluate the correlation of pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content with malignant proliferation activity of cancer cells in patients with gastric cancer. Results: Serum pepsinogenⅠ/Ⅱ ratio and gastrin-17 content in gastric cancer group were lower than those in gastric ulcer group and superficial gastritis group. Proliferation genes EIF5A2, MACF1 and PIK3CD mRNA expression levels in gastric cancer group were higher than those in gastric ulcer group and superficial gastritis group whereas SIRT1 mRNA expression level was lower than that in gastric ulcer group and superficial gastritis group;apoptosis gene Livin mRNA expression level was higher than that in gastric ulcer group and superficial gastritis group whereas Bad and Noxa mRNA expression levels were lower than those in gastric ulcer group and superficial gastritis group;serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content in gastric cancer group were negatively correlated with EIF5A2, MACF1, PIK3CD and Livin mRNA expression levels, and positively correlated with SIRT1, Bad and Noxa mRNA expression levels. Conclusion: The serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content abnormally reduce in patients with gastric cancer, and the specific levels are directly correlated with gastric cancer cell proliferation and apoptosis activity.展开更多
AIM:To investigate the associations of interleukin-17(IL-17)genetic polymorphisms and serum levels with ulcerative colitis(UC)risk.METHODS:Relevant articles were identified through a search of the following electronic...AIM:To investigate the associations of interleukin-17(IL-17)genetic polymorphisms and serum levels with ulcerative colitis(UC)risk.METHODS:Relevant articles were identified through a search of the following electronic databases,excluding language restriction:(1)the Cochrane Library Database(Issue 12,2013);(2)Web of Science(1945-2013);(3)PubMed(1966-2013);(4)CINAHL(1982-2013);(5)EMBASE(1980-2013);and(6)the Chinese Biomedical Database(1982-2013).Meta-analysis was conducted using STATA 12.0 software.Crude odds ratios and standardized mean differences(SMDs)with corresponding95%confidence intervals(CIs)were calculated.All of the included studies met all of the following five criteria:(1)the study design must be a clinical cohort or a case-control study;(2)the study must relate to the relationship between IL-17A/F genetic polymorphismsor serum IL-17 levels and the risk of UC;(3)all patients must meet the diagnostic criteria for UC;(4)the study must provide sufficient information about single nucleotide polymorphism frequencies or serum IL-17 levels;and(5)the genotype distribution of healthy controls must conform to the Hardy-Weinberg equilibrium(HWE).The Newcastle-Ottawa Scale(NOS)criteria were used to assess the methodological quality of the studies.The NOS criteria included three aspects:(1)subject selection:0-4;(2)comparability of subjects:0-2;and(3)clinical outcome:0-3.NOS scores ranged from 0 to 9,with a score≥7 indicating good quality.RESULTS:Of the initial 177 articles,only 16 case-control studies met all of the inclusion criteria.A total of1614 UC patients and 2863 healthy controls were included in this study.Fourteen studies were performed on Asian populations,and two studies on Caucasian populations.Results of the meta-analysis revealed that IL-17A and IL-17F genetic polymorphisms potentially increased UC risk under both allele and dominant models(P<0.001 for all).The results also showed that UC patients had higher serum IL-17 levels than healthy controls(SMD=5.95,95%CI:4.25-7.65,P<0.001).Furthermore,serum IL-17 levels significantly correlated with the severity of UC(moderate vs mild:SMD=2.59,95%CI:0.03-5.16,P<0.05;severe vs mild:SMD=7.09,95%CI:3.96-10.23,P<0.001;severe vs moderate:SMD=5.84,95%CI:5.09-6.59,P<0.001).The NOS score was≥5 for all of the included studies.Based on the sensitivity analysis,no single study influenced the overall pooled estimates.Neither the Begger’s funnel plots nor Egger’s test displayed strong statistical evidence for publication bias(IL-17A/F genetic polymorphisms:t=-2.60,P=0.019;serum IL-17 levels:t=-1.54,P=0.141).CONCLUSION:The findings strongly suggest that IL-17A/F genetic polymorphisms and serum IL-17 levels contribute to the development and progression of UC.展开更多
The aim of this study was to compare serum 17β-estradiol of menopausal women with/without Oral Dryness (OD) feeling, and evaluate the re-lationship between serum 17β-estradiol and severity of OD feeling. A case-cont...The aim of this study was to compare serum 17β-estradiol of menopausal women with/without Oral Dryness (OD) feeling, and evaluate the re-lationship between serum 17β-estradiol and severity of OD feeling. A case-control study was carried out on 70 selected menopausal women aged 40 - 77 years with or without OD feeling (35 as case, 35 as control) conducted at the Clinic of Oral Medicine, Tehran University of Medical Sciences. Xerostomia inventory (XI) score was used as an index of OD feeling severity. The serum 17β-estradiol concentration was measured by an enzyme immunoassay kit (ELISA). Statistical analysis of Student’s t-test and Spearman correlation coefficient was used. The mean serum concentration of 17β-estradiol was significantly lower in case than control. There was a significant negative correlation between XI score and concentration of 17β-estradiol in menopausal women (r = –0.311, P = 0.004). It seems that there is a negatively slight correlation between OD feeling severity and serum 17β-estradiol in menopausal women.展开更多
目的探讨血清CC趋化因子配体20(CCL20)及白介素-17(IL-17)水平与子痫前期的相关性及其对子痫前期的诊断效能。方法选择2021年1月至2022年12月陕西省人民医院产科收治的150例子痫前期孕妇为子痫前期组,另选取同期90例健康妊娠妇女为对照...目的探讨血清CC趋化因子配体20(CCL20)及白介素-17(IL-17)水平与子痫前期的相关性及其对子痫前期的诊断效能。方法选择2021年1月至2022年12月陕西省人民医院产科收治的150例子痫前期孕妇为子痫前期组,另选取同期90例健康妊娠妇女为对照组。采用酶联免疫吸附法(ELISA)检测血清CCL20及IL-17的表达水平,采用Pearson相关法分析血清CCL20及IL-17与子痫前期患者临床参数的相关性;采用受试者工作特征曲线(ROC)分析血清CCL20及IL-17对子痫前期的诊断价值。结果子痫前期组孕妇血清CCL20及IL-17水平均显著高于对照组(P<0.001)。子痫前期组孕妇血清CCL20及IL-17的表达均与收缩压(分别为r=0.463、r=0.545)、舒张压(分别为r=0.475、r=0.467)及尿蛋白(分别为r=0.302、r=0.342)呈显著正相关(P<0.001),而与终止妊娠孕周(分别为r=-0.281、r=-0.188)、新生儿出生体重(分别为r=-0.299、r=-0.200)及1 min Apgar评分(分别为r=-0.215、r=-0.194)呈显著负相关(P<0.05)。子痫前期组孕妇血清CCL20与IL-17水平呈显著正相关(r=0.615,P<0.001)。血清CCL20及IL-17诊断子痫前期的临界值分别为50.025 pg/ml及48.825 pg/ml,血清CCL20+IL-17联合诊断子痫前期的曲线下面积最大,灵敏度及特异度分别为87.3%及94.4%。结论子痫前期孕妇血清CCL20及IL-17的水平显著高于健康孕妇,且CCL20及IL-17水平与患者的血压、尿蛋白、妊娠结局及新生儿情况均存在显著相关性,两指标联合应用对子痫前期有一定的诊断价值。展开更多
文摘Objective: To investigate the correlation of serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content with malignant proliferation of cancer cells in patients with gastric cancer. Methods:Superficial gastritis group (n=247), gastric ulcer group (n=159) and gastric cancer group (n=97) who were pathologically diagnosed by gastroscopy in Chongqing wanzhou district people's hospital of 5 between August 2016 and August 2017 were selected, and the differences in serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content as well as proliferation and apoptosis gene expression in lesion tissue were compared among the three groups. Pearson test was used to evaluate the correlation of pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content with malignant proliferation activity of cancer cells in patients with gastric cancer. Results: Serum pepsinogenⅠ/Ⅱ ratio and gastrin-17 content in gastric cancer group were lower than those in gastric ulcer group and superficial gastritis group. Proliferation genes EIF5A2, MACF1 and PIK3CD mRNA expression levels in gastric cancer group were higher than those in gastric ulcer group and superficial gastritis group whereas SIRT1 mRNA expression level was lower than that in gastric ulcer group and superficial gastritis group;apoptosis gene Livin mRNA expression level was higher than that in gastric ulcer group and superficial gastritis group whereas Bad and Noxa mRNA expression levels were lower than those in gastric ulcer group and superficial gastritis group;serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content in gastric cancer group were negatively correlated with EIF5A2, MACF1, PIK3CD and Livin mRNA expression levels, and positively correlated with SIRT1, Bad and Noxa mRNA expression levels. Conclusion: The serum pepsinogen Ⅰ/Ⅱ ratio and gastrin-17 content abnormally reduce in patients with gastric cancer, and the specific levels are directly correlated with gastric cancer cell proliferation and apoptosis activity.
文摘AIM:To investigate the associations of interleukin-17(IL-17)genetic polymorphisms and serum levels with ulcerative colitis(UC)risk.METHODS:Relevant articles were identified through a search of the following electronic databases,excluding language restriction:(1)the Cochrane Library Database(Issue 12,2013);(2)Web of Science(1945-2013);(3)PubMed(1966-2013);(4)CINAHL(1982-2013);(5)EMBASE(1980-2013);and(6)the Chinese Biomedical Database(1982-2013).Meta-analysis was conducted using STATA 12.0 software.Crude odds ratios and standardized mean differences(SMDs)with corresponding95%confidence intervals(CIs)were calculated.All of the included studies met all of the following five criteria:(1)the study design must be a clinical cohort or a case-control study;(2)the study must relate to the relationship between IL-17A/F genetic polymorphismsor serum IL-17 levels and the risk of UC;(3)all patients must meet the diagnostic criteria for UC;(4)the study must provide sufficient information about single nucleotide polymorphism frequencies or serum IL-17 levels;and(5)the genotype distribution of healthy controls must conform to the Hardy-Weinberg equilibrium(HWE).The Newcastle-Ottawa Scale(NOS)criteria were used to assess the methodological quality of the studies.The NOS criteria included three aspects:(1)subject selection:0-4;(2)comparability of subjects:0-2;and(3)clinical outcome:0-3.NOS scores ranged from 0 to 9,with a score≥7 indicating good quality.RESULTS:Of the initial 177 articles,only 16 case-control studies met all of the inclusion criteria.A total of1614 UC patients and 2863 healthy controls were included in this study.Fourteen studies were performed on Asian populations,and two studies on Caucasian populations.Results of the meta-analysis revealed that IL-17A and IL-17F genetic polymorphisms potentially increased UC risk under both allele and dominant models(P<0.001 for all).The results also showed that UC patients had higher serum IL-17 levels than healthy controls(SMD=5.95,95%CI:4.25-7.65,P<0.001).Furthermore,serum IL-17 levels significantly correlated with the severity of UC(moderate vs mild:SMD=2.59,95%CI:0.03-5.16,P<0.05;severe vs mild:SMD=7.09,95%CI:3.96-10.23,P<0.001;severe vs moderate:SMD=5.84,95%CI:5.09-6.59,P<0.001).The NOS score was≥5 for all of the included studies.Based on the sensitivity analysis,no single study influenced the overall pooled estimates.Neither the Begger’s funnel plots nor Egger’s test displayed strong statistical evidence for publication bias(IL-17A/F genetic polymorphisms:t=-2.60,P=0.019;serum IL-17 levels:t=-1.54,P=0.141).CONCLUSION:The findings strongly suggest that IL-17A/F genetic polymorphisms and serum IL-17 levels contribute to the development and progression of UC.
文摘The aim of this study was to compare serum 17β-estradiol of menopausal women with/without Oral Dryness (OD) feeling, and evaluate the re-lationship between serum 17β-estradiol and severity of OD feeling. A case-control study was carried out on 70 selected menopausal women aged 40 - 77 years with or without OD feeling (35 as case, 35 as control) conducted at the Clinic of Oral Medicine, Tehran University of Medical Sciences. Xerostomia inventory (XI) score was used as an index of OD feeling severity. The serum 17β-estradiol concentration was measured by an enzyme immunoassay kit (ELISA). Statistical analysis of Student’s t-test and Spearman correlation coefficient was used. The mean serum concentration of 17β-estradiol was significantly lower in case than control. There was a significant negative correlation between XI score and concentration of 17β-estradiol in menopausal women (r = –0.311, P = 0.004). It seems that there is a negatively slight correlation between OD feeling severity and serum 17β-estradiol in menopausal women.
文摘目的探讨血清CC趋化因子配体20(CCL20)及白介素-17(IL-17)水平与子痫前期的相关性及其对子痫前期的诊断效能。方法选择2021年1月至2022年12月陕西省人民医院产科收治的150例子痫前期孕妇为子痫前期组,另选取同期90例健康妊娠妇女为对照组。采用酶联免疫吸附法(ELISA)检测血清CCL20及IL-17的表达水平,采用Pearson相关法分析血清CCL20及IL-17与子痫前期患者临床参数的相关性;采用受试者工作特征曲线(ROC)分析血清CCL20及IL-17对子痫前期的诊断价值。结果子痫前期组孕妇血清CCL20及IL-17水平均显著高于对照组(P<0.001)。子痫前期组孕妇血清CCL20及IL-17的表达均与收缩压(分别为r=0.463、r=0.545)、舒张压(分别为r=0.475、r=0.467)及尿蛋白(分别为r=0.302、r=0.342)呈显著正相关(P<0.001),而与终止妊娠孕周(分别为r=-0.281、r=-0.188)、新生儿出生体重(分别为r=-0.299、r=-0.200)及1 min Apgar评分(分别为r=-0.215、r=-0.194)呈显著负相关(P<0.05)。子痫前期组孕妇血清CCL20与IL-17水平呈显著正相关(r=0.615,P<0.001)。血清CCL20及IL-17诊断子痫前期的临界值分别为50.025 pg/ml及48.825 pg/ml,血清CCL20+IL-17联合诊断子痫前期的曲线下面积最大,灵敏度及特异度分别为87.3%及94.4%。结论子痫前期孕妇血清CCL20及IL-17的水平显著高于健康孕妇,且CCL20及IL-17水平与患者的血压、尿蛋白、妊娠结局及新生儿情况均存在显著相关性,两指标联合应用对子痫前期有一定的诊断价值。
