The 18 crude drugs in Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風通聖散) are separated into 6 groups such as diaphoretic, cathartic, antidote, antipyretic, neutralizer and diuretic groups. The effects o...The 18 crude drugs in Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風通聖散) are separated into 6 groups such as diaphoretic, cathartic, antidote, antipyretic, neutralizer and diuretic groups. The effects of single administered BOF and composed crude drugs in 6 groups were investigated on the levels of diabetic parameters (serum glucose, insulin, triglyceride and cholesterol) in streptozotocin-induced diabetic mice. The anti-hyperglycemic action of BOF was depended on Ephedrae Herba, Saposhnikoviae Radix and Schizonepetae Spica in diaphoretic group, Forsythiae Fructus, Saposhnikoviae Radix, Schizonepetae Spica and Cnidii Rhizoma in antidote group, Scutellariae Radix, Gardeniae Fructus and Gypsum Fibrosum in antipyretic group and Paeoniae Radix in neutralizer group. In these crude drugs, Ephedrae Herba, Saposhnikoviae Radix, Schizonepetae Spica, Forsythiae Fructus, Scutellariae Radix, Gypsum Fibrosum and Paeoniae Radix increased serum insulin level, but Cnidii Rhizoma and Gardeniae Fructus did not affect serum insulin level. From these results, it suggested that anti-hyperglycemic action of BOF was through insulin-dependent and insulin independent manners. The lowering effect of BOF on serum triglyceride level was dependent on actions of Platycodi Radix in antidote and diuretic groups and Gardeniae Fructus in antipyretic group. The lowering effect of Gardeniae Fructus was parallel with its anti-hyperglycemic action. The lowering effect of BOF on high serum triglyceride level also included both direct action and indirect action. The reducing effect of BOF on serum cholesterol level was observed together with the actions of Ephedrae Herba and Zingiberis Rhizoma in diaphoretic group, Schizonepetae Spica in diaphoretic and antidote groups and Paeoniae Radix in neutralizer group. The lowering effects of Ephedrae Herba, Schizonepetae Spica and Paeoniae Radix on serum cholesterol level were parallel with their anti-hyperglycemic actions. Zingiberis Rhizoma in diaphoretic group might be direct reducing effect on serum cholesterol level but no serum glucose level. The Ephedrae Herba in diaphoretic group, Schizonepetae Spica in diaphoretic and antidote groups and Paeoniae Radix in neutralizer group might have reduced serum cholesterol level by reducing blood glucose level. From these results, composed crude drugs in 6 groups show various mechanisms in the action of BOF.展开更多
目的:探讨血清高速泳动族蛋白B1(high-mobility group box-B1,HMGB1)对脓毒症患者预后评估的价值。方法:选取2018年1月至2021年12月上海交通大学医学院附属仁济医院南院收治的342例脓毒症患者作为研究对象,其中男性192例,女性150例,平...目的:探讨血清高速泳动族蛋白B1(high-mobility group box-B1,HMGB1)对脓毒症患者预后评估的价值。方法:选取2018年1月至2021年12月上海交通大学医学院附属仁济医院南院收治的342例脓毒症患者作为研究对象,其中男性192例,女性150例,平均年龄(60.54±17.85)岁。根据28 d预后结局分为生存组和死亡组,收集各种临床资料,并测定入院第1天的HMGB1水平。利用COX多元回归分析脓毒症患者预后不良的危险因素。采用Pearson分析血清HMGB1与序贯器官衰竭评估(sequential organ failure assessment,SOFA)评分、急性生理学和慢性健康状况评价Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)评分的相关性。绘制受试者操作特征曲线(receiver operator characteristic curve,ROC曲线),分析血清HMGB1评估脓毒症预后的曲线下面积(area under the curve,AUC)及截断值。根据ROC曲线确定的血清HMGB1截断值将患者分成高HMGB1水平组、低HMGB1水平组,绘制Kaplan Meier生存曲线图,进一步分析血清HMGB1与预后的关系。结果:所有入组患者的死亡率为29.2%(100例)。死亡组中血清HMGB1、C反应蛋白、降钙素原、白细胞计数、乳酸水平、SOFA及APACHEⅡ评分、呼吸道感染、并发脓毒症休克及弥散性血管内凝血、接受机械通气的比例均明显高于生存组(均P<0.05),合并糖尿病、泌尿道感染比例明显低于生存组(均P<0.05)。多元回归分析显示血清HMGB1水平、SOFA评分、白细胞计数、呼吸道感染均是脓毒症28 d死亡的影响因素。Pearson相关性分析发现脓毒症患者血清HMGB1水平与SOFA评分、APACHEⅡ评分呈正相关(P<0.001)。ROC曲线显示HMGB1、SOFA评分、APACHEⅡ评分预测脓毒症28 d死亡的AUC分别为0.776、0.774、0.760。Kaplan Meier生存曲线发现高HMGB1水平组患者的28 d死亡率明显高于低HMGB1水平组(P<0.001)。结论:血清HMGB1水平在脓毒症死亡组中明显升高,是预测脓毒症28 d死亡的危险因素之一,其预测效能不劣于SOFA评分、APACHEⅡ评分。展开更多
文摘The 18 crude drugs in Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風通聖散) are separated into 6 groups such as diaphoretic, cathartic, antidote, antipyretic, neutralizer and diuretic groups. The effects of single administered BOF and composed crude drugs in 6 groups were investigated on the levels of diabetic parameters (serum glucose, insulin, triglyceride and cholesterol) in streptozotocin-induced diabetic mice. The anti-hyperglycemic action of BOF was depended on Ephedrae Herba, Saposhnikoviae Radix and Schizonepetae Spica in diaphoretic group, Forsythiae Fructus, Saposhnikoviae Radix, Schizonepetae Spica and Cnidii Rhizoma in antidote group, Scutellariae Radix, Gardeniae Fructus and Gypsum Fibrosum in antipyretic group and Paeoniae Radix in neutralizer group. In these crude drugs, Ephedrae Herba, Saposhnikoviae Radix, Schizonepetae Spica, Forsythiae Fructus, Scutellariae Radix, Gypsum Fibrosum and Paeoniae Radix increased serum insulin level, but Cnidii Rhizoma and Gardeniae Fructus did not affect serum insulin level. From these results, it suggested that anti-hyperglycemic action of BOF was through insulin-dependent and insulin independent manners. The lowering effect of BOF on serum triglyceride level was dependent on actions of Platycodi Radix in antidote and diuretic groups and Gardeniae Fructus in antipyretic group. The lowering effect of Gardeniae Fructus was parallel with its anti-hyperglycemic action. The lowering effect of BOF on high serum triglyceride level also included both direct action and indirect action. The reducing effect of BOF on serum cholesterol level was observed together with the actions of Ephedrae Herba and Zingiberis Rhizoma in diaphoretic group, Schizonepetae Spica in diaphoretic and antidote groups and Paeoniae Radix in neutralizer group. The lowering effects of Ephedrae Herba, Schizonepetae Spica and Paeoniae Radix on serum cholesterol level were parallel with their anti-hyperglycemic actions. Zingiberis Rhizoma in diaphoretic group might be direct reducing effect on serum cholesterol level but no serum glucose level. The Ephedrae Herba in diaphoretic group, Schizonepetae Spica in diaphoretic and antidote groups and Paeoniae Radix in neutralizer group might have reduced serum cholesterol level by reducing blood glucose level. From these results, composed crude drugs in 6 groups show various mechanisms in the action of BOF.
文摘目的:探讨血清高速泳动族蛋白B1(high-mobility group box-B1,HMGB1)对脓毒症患者预后评估的价值。方法:选取2018年1月至2021年12月上海交通大学医学院附属仁济医院南院收治的342例脓毒症患者作为研究对象,其中男性192例,女性150例,平均年龄(60.54±17.85)岁。根据28 d预后结局分为生存组和死亡组,收集各种临床资料,并测定入院第1天的HMGB1水平。利用COX多元回归分析脓毒症患者预后不良的危险因素。采用Pearson分析血清HMGB1与序贯器官衰竭评估(sequential organ failure assessment,SOFA)评分、急性生理学和慢性健康状况评价Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)评分的相关性。绘制受试者操作特征曲线(receiver operator characteristic curve,ROC曲线),分析血清HMGB1评估脓毒症预后的曲线下面积(area under the curve,AUC)及截断值。根据ROC曲线确定的血清HMGB1截断值将患者分成高HMGB1水平组、低HMGB1水平组,绘制Kaplan Meier生存曲线图,进一步分析血清HMGB1与预后的关系。结果:所有入组患者的死亡率为29.2%(100例)。死亡组中血清HMGB1、C反应蛋白、降钙素原、白细胞计数、乳酸水平、SOFA及APACHEⅡ评分、呼吸道感染、并发脓毒症休克及弥散性血管内凝血、接受机械通气的比例均明显高于生存组(均P<0.05),合并糖尿病、泌尿道感染比例明显低于生存组(均P<0.05)。多元回归分析显示血清HMGB1水平、SOFA评分、白细胞计数、呼吸道感染均是脓毒症28 d死亡的影响因素。Pearson相关性分析发现脓毒症患者血清HMGB1水平与SOFA评分、APACHEⅡ评分呈正相关(P<0.001)。ROC曲线显示HMGB1、SOFA评分、APACHEⅡ评分预测脓毒症28 d死亡的AUC分别为0.776、0.774、0.760。Kaplan Meier生存曲线发现高HMGB1水平组患者的28 d死亡率明显高于低HMGB1水平组(P<0.001)。结论:血清HMGB1水平在脓毒症死亡组中明显升高,是预测脓毒症28 d死亡的危险因素之一,其预测效能不劣于SOFA评分、APACHEⅡ评分。
