目的:分析血清IL-33及IL-37对不同水平血嗜酸性粒细胞的慢性阻塞性肺疾病患者肺功能的预测价值及相关性。方法:采用ELISA法检测IL-33、IL-37、CRP及PCT在30例嗜酸性粒细胞丰富组患者、30例嗜酸性粒细胞中间组患者、34例嗜酸性粒细胞匮...目的:分析血清IL-33及IL-37对不同水平血嗜酸性粒细胞的慢性阻塞性肺疾病患者肺功能的预测价值及相关性。方法:采用ELISA法检测IL-33、IL-37、CRP及PCT在30例嗜酸性粒细胞丰富组患者、30例嗜酸性粒细胞中间组患者、34例嗜酸性粒细胞匮乏组患者及30例健康体检者血清中的表达情况,采用肺功能仪检测30例嗜酸性粒细胞丰富组患者、30例嗜酸性粒细胞中间组患者、34例嗜酸性粒细胞匮乏组患者及30例健康体检者的FEV1%pred及FEV1/FVC。结果:COPD组患者血清IL-33、IL-37、CRP及PCT表达水平较健康对照组显著升高,肺功能指标FEV1%pred及FEV1/FVC较对照组显著降低(P 1%pred及FEV1/FVC进一步降低(P 1%pred、FEV1/FVC呈负相关(P Objective: To analyze the predictive value and correlation of serum IL-33 and IL-37 in patients with chronic obstructive pulmonary disease (COPD) with different levels of blood eosinophils in predicting lung function. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of IL-33, IL-37, C-reactive protein (CRP), and procalcitonin (PCT) in the serum of 30 patients with high eosinophil counts, 30 patients with moderate eosinophil counts, 34 patients with low eosinophil counts, and 30 healthy individuals. Lung function was assessed by measuring forced expiratory volume in one second as a percentage of predicted (FEV1%pred) and the ratio of FEV1 to forced vital capacity (FEV1/FVC) using a spirometer in the same groups. Results: The expression levels of serum IL-33, IL-37, CRP, and PCT in the COPD group were significantly higher than those in the healthy control group (P 1%pred and FEV1/FVC were significantly lower than those in the control group (P 1%pred and FEV1/FVC further decreased (P 1%pred and FEV1/FVC (P < 0.05). Conclusion: IL-33 and IL-37 may be closely related to the progression of COPD and may become new indicators for clinical diagnosis and assessment of disease severity in COPD, as well as providing new targets for clinical intervention.展开更多
Objective To observe the effect of Bufei Granules on the mild-to-moderate chronic obstructive pulmonary disease(COPD)stable phase patients,and to explore its mechanism from the perspective of regulating the serum leve...Objective To observe the effect of Bufei Granules on the mild-to-moderate chronic obstructive pulmonary disease(COPD)stable phase patients,and to explore its mechanism from the perspective of regulating the serum levels of interleukin-33(IL-33)/soluble ST2(s ST2)axis and related inflammatory cytokines.Methods A multicenter double-blinded randomized controlled trial was performed.Totally 271 mild-to-moderate COPD stable phase patients from 4 referral hospitals were enrolled and assigned to two groups by stratified block randomization,136 in the treatment group(TG)and 135 in the control group(CG).All the patients received disease education,self-management and integrative care.Additionally,the patients in the TG were treated with Bufei Granules,while the patients in the CG were treated with Bufei Granules placebo,10 g per package,2 packages each time,2 times per day.The therapeutic course for all was 12 weeks.The scores of COPD assessment test questionnaire(CATQ),St.George's Respiratory Questionnaire(SGRQ)and traditional Chinese medicine clinical symptoms(TCMCS)were recorded.The pulmonary function tests including forced expiratory volume in one second(FEV1),forced vital capacity(FVC),FEV1/predicted value(FEV1%)and FEV1/FVC,and the serum levels of IL-33,s ST2,IL-4 and IL-10 of the patients were measured.Results Compared with those before treatment,the scores of CATQ,TCMCS,SGRQ(including the scores of respiratory symptoms,activity limitation,disease affect and total score),and the serum level of s ST2 in the TG decreased obviously after treatment(P<0.01),however the serum levels of IL-33,IL-4 and IL-10 increased significantly(P<0.01),meanwhile were superior to those in the CG(P<0.01).Compared between the two groups,the indexes of FEV1,FVC,FEV1% and FEV1/FVC of the TG were higher than those in the CG after treatment(P<0.05).The total effective rate of TCMCS in the TG was 75.74%(103/136),and was superior to 9.63% in the CG(13/135,X^2=120.929,P<0.01).Conclusion Bufei Granules could effectively treat the mild-to-moderate COPD stable phase patients by ameliorating the clinical symptoms,protecting the pulmonary function from deterioration and improving the life quality.Its effect mechanism might be related to the enhancment of anti-inflammation ability by increasing the serum levels of IL-33,IL-4 and IL-10,and decreasing the serum level of s ST2.展开更多
文摘目的:分析血清IL-33及IL-37对不同水平血嗜酸性粒细胞的慢性阻塞性肺疾病患者肺功能的预测价值及相关性。方法:采用ELISA法检测IL-33、IL-37、CRP及PCT在30例嗜酸性粒细胞丰富组患者、30例嗜酸性粒细胞中间组患者、34例嗜酸性粒细胞匮乏组患者及30例健康体检者血清中的表达情况,采用肺功能仪检测30例嗜酸性粒细胞丰富组患者、30例嗜酸性粒细胞中间组患者、34例嗜酸性粒细胞匮乏组患者及30例健康体检者的FEV1%pred及FEV1/FVC。结果:COPD组患者血清IL-33、IL-37、CRP及PCT表达水平较健康对照组显著升高,肺功能指标FEV1%pred及FEV1/FVC较对照组显著降低(P 1%pred及FEV1/FVC进一步降低(P 1%pred、FEV1/FVC呈负相关(P Objective: To analyze the predictive value and correlation of serum IL-33 and IL-37 in patients with chronic obstructive pulmonary disease (COPD) with different levels of blood eosinophils in predicting lung function. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of IL-33, IL-37, C-reactive protein (CRP), and procalcitonin (PCT) in the serum of 30 patients with high eosinophil counts, 30 patients with moderate eosinophil counts, 34 patients with low eosinophil counts, and 30 healthy individuals. Lung function was assessed by measuring forced expiratory volume in one second as a percentage of predicted (FEV1%pred) and the ratio of FEV1 to forced vital capacity (FEV1/FVC) using a spirometer in the same groups. Results: The expression levels of serum IL-33, IL-37, CRP, and PCT in the COPD group were significantly higher than those in the healthy control group (P 1%pred and FEV1/FVC were significantly lower than those in the control group (P 1%pred and FEV1/FVC further decreased (P 1%pred and FEV1/FVC (P < 0.05). Conclusion: IL-33 and IL-37 may be closely related to the progression of COPD and may become new indicators for clinical diagnosis and assessment of disease severity in COPD, as well as providing new targets for clinical intervention.
文摘Objective To observe the effect of Bufei Granules on the mild-to-moderate chronic obstructive pulmonary disease(COPD)stable phase patients,and to explore its mechanism from the perspective of regulating the serum levels of interleukin-33(IL-33)/soluble ST2(s ST2)axis and related inflammatory cytokines.Methods A multicenter double-blinded randomized controlled trial was performed.Totally 271 mild-to-moderate COPD stable phase patients from 4 referral hospitals were enrolled and assigned to two groups by stratified block randomization,136 in the treatment group(TG)and 135 in the control group(CG).All the patients received disease education,self-management and integrative care.Additionally,the patients in the TG were treated with Bufei Granules,while the patients in the CG were treated with Bufei Granules placebo,10 g per package,2 packages each time,2 times per day.The therapeutic course for all was 12 weeks.The scores of COPD assessment test questionnaire(CATQ),St.George's Respiratory Questionnaire(SGRQ)and traditional Chinese medicine clinical symptoms(TCMCS)were recorded.The pulmonary function tests including forced expiratory volume in one second(FEV1),forced vital capacity(FVC),FEV1/predicted value(FEV1%)and FEV1/FVC,and the serum levels of IL-33,s ST2,IL-4 and IL-10 of the patients were measured.Results Compared with those before treatment,the scores of CATQ,TCMCS,SGRQ(including the scores of respiratory symptoms,activity limitation,disease affect and total score),and the serum level of s ST2 in the TG decreased obviously after treatment(P<0.01),however the serum levels of IL-33,IL-4 and IL-10 increased significantly(P<0.01),meanwhile were superior to those in the CG(P<0.01).Compared between the two groups,the indexes of FEV1,FVC,FEV1% and FEV1/FVC of the TG were higher than those in the CG after treatment(P<0.05).The total effective rate of TCMCS in the TG was 75.74%(103/136),and was superior to 9.63% in the CG(13/135,X^2=120.929,P<0.01).Conclusion Bufei Granules could effectively treat the mild-to-moderate COPD stable phase patients by ameliorating the clinical symptoms,protecting the pulmonary function from deterioration and improving the life quality.Its effect mechanism might be related to the enhancment of anti-inflammation ability by increasing the serum levels of IL-33,IL-4 and IL-10,and decreasing the serum level of s ST2.
