In order to provide reference for using Siganus guttatus to control overgrowth of Enteromorpha prolifra,the growth,serum biochemical and antioxidant enzyme indices of juvenile S.guttatus respectively fed with E.prolif...In order to provide reference for using Siganus guttatus to control overgrowth of Enteromorpha prolifra,the growth,serum biochemical and antioxidant enzyme indices of juvenile S.guttatus respectively fed with E.prolifra and artificial feed were studied.One hundred and eighty individuals were cultivated for 90 days in six 2.5 m × 1.5 m × 1.5 m cages (30 ind.per cage) which mesh size were 0.5 mm.The experimental animals were divided into two dietary groups (three cages for each group) that were fed with E.prolifra and artificial feed respectively.During the culture period,temperatures ranged from 23.0 to 26.5℃,p H was between 7.8 and 8.2,dissolved oxygen was more than 5.0 mg/L.The weight gain rate,specific growth rate,relative growth rate,hepato-somatic index of juvenile S.guttatus fed with E.prolifra were significantly lowe than that fed with artificial feed (P<0.05).The total protein (TP),urea nitrogen (UN)and alkaline phosphatase (AKP) of juvenile S.guttatus fed with E.Prolifra were significantly higher than that fed with artificial feed (P<0.05),while glutamic oxalacetic transaminase (AST/GOT) and glutamic-pyruvic transaminase (ALT/GPT) of juvenile S.guttatus fed with E.prolifra were significantly lower than that fed with artificial feed (P<0.05).SOD,GPX and anti-superoxide anion radical activity in liver,kidney,gill and muscle of juvenile S.guttatus fed with E.prolifra were higher than that fed with artificial feed,and these antioxidant enzyme activities in kidney of juvenile S.guttatus fed with E.prolifra was significantly higher than that fed with artificial feed (P<0.05),while CAT and hydroxyl radical-inhibiting activity in liver of juvenile S.guttatus fed with artificial feed were significantly higher than that fed with E.prolifra (P<0.05).Juvenile S.guttatus fed with E.prolifra showed poor growth performance but better antioxidant defense system.S.guttatus is easily raised and has stronger ability of digestion and absorption of E.Prolifra.The technique that uses S.guttatus to control E.prolifra deserves deeply study.展开更多
Objective To examine the utility of the Nutrition Risk Screening 2002(NRS 2002),Patient-Generated Subjective Global Assessment(PG-SGA)and serum factors for the preoperative evaluation of gastric cancer patients.Method...Objective To examine the utility of the Nutrition Risk Screening 2002(NRS 2002),Patient-Generated Subjective Global Assessment(PG-SGA)and serum factors for the preoperative evaluation of gastric cancer patients.Methods We examined 181 gastric cancer patients treated at the First Affiliated Hospital of Guangxi Medical University from January 2015 to January 2018.Nutritional assessments were administered within 48 h of admission.The body mass index(BMI)and serum factors were measured,and information on preoperative nutritional support and postoperative complications was recorded.Results Both the NRS 2002 and PG-SGA had positive correlations with age(P<0.05),and negative correlations with albumin,prealbumin,transferrin,hemoglobin,BMI,and bodyweight(P<0.05).The NRS 2002 and PG-SGA scores were positively correlated with each other(r=0.683,P<0.01),but had poor consistency(κ=0.357,P<0.01).During the preoperative period,33.2%of patients received nutritional support,mainly enteral nutrition.The nutritional risk group(NRS 2002≥3)received more support than the group without nutritional risk(NRS 2002<3;P<0.05).Patients with nutritional risk or malnutrition who received preoperative nutritional support had fewer postoperative complications than unsupported patients.The overall rate of complications was 12.2%,and the rate of severe complications(gradeⅢor above)was 5.5%.The malnutrition and nutritional risk groups had higher rates of severe complications.Conclusions The combined application of the NRS 2002,PG-SGA,BMI,and serum nutritional indices was useful for the nutritional screening and assessment of preoperative gastric cancer patients.The NRS 2002 and PG-SGA provided guidance on the need for nutritional support during the preoperative period.展开更多
The objective of the present study was to investigate the effects of methionine(Met) supplementation on growth performance of cubs, nutrient digestibility, nitrogen metabolism and serum biochemical parameters of femal...The objective of the present study was to investigate the effects of methionine(Met) supplementation on growth performance of cubs, nutrient digestibility, nitrogen metabolism and serum biochemical parameters of female blue foxes. One hundred primiparous female blue foxes that were similar in breeding date, pedigree, age, and weight were selected for the trial. The foxes were randomly assigned to four groups(n = 25 each group) and fed diets supplemented with Met at 2(Met2), 4(Met4), 6(Met6)and 8 g/kg(Met8), respectively, for 40 days. Our data showed that body weights at 20 and 40 d were significantly higher in the Met4 group than in the Met2 group(P < 0.05). The Met4 group also had the highest apparent digestibility of dry matter and crude protein compared with either the Met2, Met6, or Met8 group(P< 0.05). The serum Met and isoleucine(Ile) concentrations were significantly higher in the Met4 group than in the Met6 or Met8 group(P < 0.05). In summary, these data indicate that supplementary Met improves growth performance of cubs likely due to increased crude protein and dry matter and increased nitrogen retention of female blue foxes. The optimal amount of Met supplementation is10 g/kg basal diet.展开更多
Objective: To evaluate the ameliorating effects of Raphanus sativus leaves(RSL)against sodium arsenite(Sa)-induced adverse effects through mice experiments.Methods: Swiss albino mice were divided into four equal group...Objective: To evaluate the ameliorating effects of Raphanus sativus leaves(RSL)against sodium arsenite(Sa)-induced adverse effects through mice experiments.Methods: Swiss albino mice were divided into four equal groups: control, Sa, RSL,RSL + Sa. Sa(10 mg/kg body weight/day), and powder form of RSL(50 mg/kg body weight/day) were provided as food supplement orallty. Blood indices were measured using commercially available kits through colorimetric methods.Results: It was observed that lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase activities were significantly(P < 0.05)higher in Sa-treated mice than those in the control group. RSL significantly reduced Sainduced elevation of the activities of these enzymes in serum significantly(P < 0.05).Serum butyrylcholinesterase activity and high density lipoproteins cholesterol levels in Satreated mice were significantly(P < 0.05) lower than the control group, and the food supplementation of RSL could significantly(P < 0.05) prevent the reduction of Sa-mediated serum butyryl cholinesterase activity and high density lipoproteins cholesterol levels. RSL could also reduce the Sa-induced elevation of serum urea level significantly(P < 0.05).Conclusions: Results of this study suggest the protective or ameliorating effects of RSL on Sa-induced perturbation of blood indices are related to the hepatic, cardiovascular and kidney dysfunction. Therefore, RSL may be useful to reduce arsenic toxicity in human in the future.展开更多
<i><span style="font-family:Verdana;">Mucuna pruriens</span></i><span style="font-family:Verdana;"> (velvet bean) represents an interesting source of protein poorly st...<i><span style="font-family:Verdana;">Mucuna pruriens</span></i><span style="font-family:Verdana;"> (velvet bean) represents an interesting source of protein poorly studied. The effect of dietary inclusion of meal and protein isolate of <i></i></span><i><i><span style="font-family:Verdana;">Mucuna</span></i><span style="font-family:Verdana;"></span></i> seeds on biochemical and oxidative stress parameter of broilers (135-one-day Cobb<sub>500</sub> chickens) was investigated. Three isonitrogenous diets were formulated from soy<span style="font-family:Verdana;">a </span><span style="font-family:;" "=""><span style="font-family:Verdana;">bean meal (Control group: RTS), <i></i></span><i><i><span style="font-family:Verdana;">Mucuna</span></i><span style="font-family:Verdana;"></span></i> meal (coded RFM) and <i></i></span><i><i><span style="font-family:Verdana;">Mucuna</span></i><span style="font-family:Verdana;"></span></i> protein isolate (coded RIM). Each of the dietary treatments was triplicated with 15 birds per replicate in a completely randomized design. The birds were offered feed and water <i><span style="font-family:Verdana;">ad </span></i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">libitum</span></i><span style="font-family:Verdana;"></span></i>. The results revealed significant (p<span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">0.05) effect of N source on the organ total proteins with treatment RFM and RIM exhibiting lower but comparable levels in the Liver (2.01 and 1.98 g/dL), Heart (1.95 and 1.89 g/dL) and Kidney (1.92 and 1.91</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">g/dL). Triglycerides contents were significantly (p</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">0.05) higher in the liver of broilers fed RIM and RFM (2.49 and 2.36 mg/dL), in the Kidney of chicks fed RIM and RTS (2.27 and 2.34 mg/dL) and in the Heart of birds fed RTS and RFM (1.90 and 1.87 mg/dL). Broilers fed RFM presented the highest (p</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">0.05) Liver total cholesterol (1.61 mg/dL) and ALAT contents but with similar values with birds fed RTS (36.43 and 35.50 UI/L respectively). ASAT level was significantly high (p</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">0.05) in the Liver and Plasma (265.50 and 264.50 UI/L respectively) of broilers of RFM diet. In all the organs, MDA content was highest (p</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">0.05) in chicks of RIM batch. In the Heart and Plasma, chicks of RFM (3.23 and 5.05 μl/mg respectively) and RIM (5.45 and 5.35 μl/mg respectively) diets registered elevated rate of CAT. In view of these results, investigations remain to be carried out on the impact of the inclusion of </span><span><span style="font-family:Verdana;">meal and protein isolate of </span><i><span style="font-family:Verdana;">M. </span></i></span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">pruriens</span></i><span style="font-family:Verdana;"></span></i></span><span style="font-family:Verdana;"> seeds in broiler’s diet during the</span><span style="font-family:Verdana;"> growth-</span><span style="font-family:Verdana;">finishing phase</span><span style="font-family:Verdana;">.</span>展开更多
This experiment was conducted to investigate the effects of dietary arginine levels on growth performance, body composition, serum biochemical indices and resistance ability against ammonia-nitrogen stress in juvenile...This experiment was conducted to investigate the effects of dietary arginine levels on growth performance, body composition, serum biochemical indices and resistance ability against ammonia-nitrogen stress in juvenile yellow catfish(Pelteobagrus fulvidraco). Five isonitrogenous and isolipidic diets(42%protein and 9% lipid) were formulated to contain graded levels of arginine(2.44%, 2.64%, 2.81%, 3.01% and3.23% of diet), by supplementing L-Arginine HCI. Seven hundred juvenile yellow catfish with an initial average body weight of 1.13 ± 0.02 g were randomly divided into 5 groups with 4 replicates of 35 fish each and each group was fed one of the diets. After 56 d feeding, fish were exposed to 100 mg/L of ammonia-nitrogen for 72 h. The results showed that weight gain(WG) and specific growth rate(SGR) in2.64% and 2.81% groups were significantly higher than those in 3.23% group(P < 0.05). The feed conversation ratio(FCR) in 2,64%, 2,81% and 3.01% groups was significantly decreased when compared with3,23% group. The protein efficiency ratio(PER) in 2.64% group was significantly higher than that in 2.44%and 3.23% groups(P < 0.05). The condition factor(CF) of fish was significantly higher in 2.81% group than that in 2,44% group(P < 0.05). Dietary arginine levels had no significant effect on hepatosomatic index(HSI), viscerosomatic index(VSI), and whole-body dry matter, crude protein, crude lipid, ash contents, as well as serum total protein(TP), triglyceride(TG), glucose(GLU), urea nitrogen(UN) contents and aspartate aminotransferase(AST), alanine aminotransferase(ALT) activities(P> 0.05). After the fish were challenged to ammonia-nitrogen for 72 h, their cumulative mortality rate in 2.81% group was significantly lower than that in 2.44% group(P < 0.05). The results suggested that dietary arginine level at 2.81%could optimize anti-ammonia-nitrogen stress ability of juvenile yellow catfish and a level of 3.23%arginine seemed to depress the growth performance of fish and decreased their tolerance to the ammonia-nitrogen stress under current study. A quadratic regression analysis based on WG indicated that the optimal dietary arginine requirement of juvenile yellow catfish was estimated to be 2.74% of the diet(6.45% of dietary protein) under current culture conditions.展开更多
BACKGROUND According to the indexes of serum and anal function,acupuncture therapy was applied to patients with low rectal cancer in order to avoid the occurrence of anal incontinence and reduce complications.AIM To e...BACKGROUND According to the indexes of serum and anal function,acupuncture therapy was applied to patients with low rectal cancer in order to avoid the occurrence of anal incontinence and reduce complications.AIM To explore the clinical application and evaluate the effect of acupuncture therapy for anal function rehabilitation after low-tension rectal cancer surgery.METHODS From the anorectal surgery cases,we selected 120 patients who underwent colorectal cancer surgery between January 2020 and December 2022 and randomly divided them into a control group(n=60),observation group(n=60),and control group after surgery for lifestyle intervention(including smoking cessation and exercise),dietary factor adjustment,anal movement,and oral loperamide treatment.The serum levels of motilin,5-hydroxytryptamine,and vasoactive intestinal peptide(VIP),Wexner score for anal incontinence,and incidence of complications were compared between groups.RESULTS After treatment,the VIP and 5-hydroxytryptamine levels in the observation group were lower than those in the control group(P<0.05).The motilin level was higher than that in the control group(P<0.05).Postoperative anal incontinence was better in the observation group than in the control group(P<0.05).The incidence of complications in the observation group was 6.67%,which was significantly lower than that in the control group(21.67%;P<0.05).CONCLUSION Acupuncture therapy has a positive effect on the rehabilitation of anal function after low-tension rectal cancer surgery;it can effectively help to improve the serum indices of patients,avoid the occurrence of anal incontinence,and reduce the incidence of complications.Popularizing and applying it will be valuable.展开更多
文摘In order to provide reference for using Siganus guttatus to control overgrowth of Enteromorpha prolifra,the growth,serum biochemical and antioxidant enzyme indices of juvenile S.guttatus respectively fed with E.prolifra and artificial feed were studied.One hundred and eighty individuals were cultivated for 90 days in six 2.5 m × 1.5 m × 1.5 m cages (30 ind.per cage) which mesh size were 0.5 mm.The experimental animals were divided into two dietary groups (three cages for each group) that were fed with E.prolifra and artificial feed respectively.During the culture period,temperatures ranged from 23.0 to 26.5℃,p H was between 7.8 and 8.2,dissolved oxygen was more than 5.0 mg/L.The weight gain rate,specific growth rate,relative growth rate,hepato-somatic index of juvenile S.guttatus fed with E.prolifra were significantly lowe than that fed with artificial feed (P<0.05).The total protein (TP),urea nitrogen (UN)and alkaline phosphatase (AKP) of juvenile S.guttatus fed with E.Prolifra were significantly higher than that fed with artificial feed (P<0.05),while glutamic oxalacetic transaminase (AST/GOT) and glutamic-pyruvic transaminase (ALT/GPT) of juvenile S.guttatus fed with E.prolifra were significantly lower than that fed with artificial feed (P<0.05).SOD,GPX and anti-superoxide anion radical activity in liver,kidney,gill and muscle of juvenile S.guttatus fed with E.prolifra were higher than that fed with artificial feed,and these antioxidant enzyme activities in kidney of juvenile S.guttatus fed with E.prolifra was significantly higher than that fed with artificial feed (P<0.05),while CAT and hydroxyl radical-inhibiting activity in liver of juvenile S.guttatus fed with artificial feed were significantly higher than that fed with E.prolifra (P<0.05).Juvenile S.guttatus fed with E.prolifra showed poor growth performance but better antioxidant defense system.S.guttatus is easily raised and has stronger ability of digestion and absorption of E.Prolifra.The technique that uses S.guttatus to control E.prolifra deserves deeply study.
基金This work was supported by a Medical and Health Suitable Technology Development and Extension Project of Guangxi Province:The combined application of the evaluation and screening of nutritional risk and serum biochemical indexes for nutritional therapy of gastric cancer patients in perioperative period(No.S2017018).
文摘Objective To examine the utility of the Nutrition Risk Screening 2002(NRS 2002),Patient-Generated Subjective Global Assessment(PG-SGA)and serum factors for the preoperative evaluation of gastric cancer patients.Methods We examined 181 gastric cancer patients treated at the First Affiliated Hospital of Guangxi Medical University from January 2015 to January 2018.Nutritional assessments were administered within 48 h of admission.The body mass index(BMI)and serum factors were measured,and information on preoperative nutritional support and postoperative complications was recorded.Results Both the NRS 2002 and PG-SGA had positive correlations with age(P<0.05),and negative correlations with albumin,prealbumin,transferrin,hemoglobin,BMI,and bodyweight(P<0.05).The NRS 2002 and PG-SGA scores were positively correlated with each other(r=0.683,P<0.01),but had poor consistency(κ=0.357,P<0.01).During the preoperative period,33.2%of patients received nutritional support,mainly enteral nutrition.The nutritional risk group(NRS 2002≥3)received more support than the group without nutritional risk(NRS 2002<3;P<0.05).Patients with nutritional risk or malnutrition who received preoperative nutritional support had fewer postoperative complications than unsupported patients.The overall rate of complications was 12.2%,and the rate of severe complications(gradeⅢor above)was 5.5%.The malnutrition and nutritional risk groups had higher rates of severe complications.Conclusions The combined application of the NRS 2002,PG-SGA,BMI,and serum nutritional indices was useful for the nutritional screening and assessment of preoperative gastric cancer patients.The NRS 2002 and PG-SGA provided guidance on the need for nutritional support during the preoperative period.
文摘The objective of the present study was to investigate the effects of methionine(Met) supplementation on growth performance of cubs, nutrient digestibility, nitrogen metabolism and serum biochemical parameters of female blue foxes. One hundred primiparous female blue foxes that were similar in breeding date, pedigree, age, and weight were selected for the trial. The foxes were randomly assigned to four groups(n = 25 each group) and fed diets supplemented with Met at 2(Met2), 4(Met4), 6(Met6)and 8 g/kg(Met8), respectively, for 40 days. Our data showed that body weights at 20 and 40 d were significantly higher in the Met4 group than in the Met2 group(P < 0.05). The Met4 group also had the highest apparent digestibility of dry matter and crude protein compared with either the Met2, Met6, or Met8 group(P< 0.05). The serum Met and isoleucine(Ile) concentrations were significantly higher in the Met4 group than in the Met6 or Met8 group(P < 0.05). In summary, these data indicate that supplementary Met improves growth performance of cubs likely due to increased crude protein and dry matter and increased nitrogen retention of female blue foxes. The optimal amount of Met supplementation is10 g/kg basal diet.
基金financially supported by the University of Rajshahi[No.A-892-5/52/BIMOK/BIGGAN(1)/2011]
文摘Objective: To evaluate the ameliorating effects of Raphanus sativus leaves(RSL)against sodium arsenite(Sa)-induced adverse effects through mice experiments.Methods: Swiss albino mice were divided into four equal groups: control, Sa, RSL,RSL + Sa. Sa(10 mg/kg body weight/day), and powder form of RSL(50 mg/kg body weight/day) were provided as food supplement orallty. Blood indices were measured using commercially available kits through colorimetric methods.Results: It was observed that lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase activities were significantly(P < 0.05)higher in Sa-treated mice than those in the control group. RSL significantly reduced Sainduced elevation of the activities of these enzymes in serum significantly(P < 0.05).Serum butyrylcholinesterase activity and high density lipoproteins cholesterol levels in Satreated mice were significantly(P < 0.05) lower than the control group, and the food supplementation of RSL could significantly(P < 0.05) prevent the reduction of Sa-mediated serum butyryl cholinesterase activity and high density lipoproteins cholesterol levels. RSL could also reduce the Sa-induced elevation of serum urea level significantly(P < 0.05).Conclusions: Results of this study suggest the protective or ameliorating effects of RSL on Sa-induced perturbation of blood indices are related to the hepatic, cardiovascular and kidney dysfunction. Therefore, RSL may be useful to reduce arsenic toxicity in human in the future.
文摘<i><span style="font-family:Verdana;">Mucuna pruriens</span></i><span style="font-family:Verdana;"> (velvet bean) represents an interesting source of protein poorly studied. The effect of dietary inclusion of meal and protein isolate of <i></i></span><i><i><span style="font-family:Verdana;">Mucuna</span></i><span style="font-family:Verdana;"></span></i> seeds on biochemical and oxidative stress parameter of broilers (135-one-day Cobb<sub>500</sub> chickens) was investigated. Three isonitrogenous diets were formulated from soy<span style="font-family:Verdana;">a </span><span style="font-family:;" "=""><span style="font-family:Verdana;">bean meal (Control group: RTS), <i></i></span><i><i><span style="font-family:Verdana;">Mucuna</span></i><span style="font-family:Verdana;"></span></i> meal (coded RFM) and <i></i></span><i><i><span style="font-family:Verdana;">Mucuna</span></i><span style="font-family:Verdana;"></span></i> protein isolate (coded RIM). Each of the dietary treatments was triplicated with 15 birds per replicate in a completely randomized design. The birds were offered feed and water <i><span style="font-family:Verdana;">ad </span></i><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">libitum</span></i><span style="font-family:Verdana;"></span></i>. The results revealed significant (p<span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">0.05) effect of N source on the organ total proteins with treatment RFM and RIM exhibiting lower but comparable levels in the Liver (2.01 and 1.98 g/dL), Heart (1.95 and 1.89 g/dL) and Kidney (1.92 and 1.91</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">g/dL). Triglycerides contents were significantly (p</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">0.05) higher in the liver of broilers fed RIM and RFM (2.49 and 2.36 mg/dL), in the Kidney of chicks fed RIM and RTS (2.27 and 2.34 mg/dL) and in the Heart of birds fed RTS and RFM (1.90 and 1.87 mg/dL). Broilers fed RFM presented the highest (p</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">0.05) Liver total cholesterol (1.61 mg/dL) and ALAT contents but with similar values with birds fed RTS (36.43 and 35.50 UI/L respectively). ASAT level was significantly high (p</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">0.05) in the Liver and Plasma (265.50 and 264.50 UI/L respectively) of broilers of RFM diet. In all the organs, MDA content was highest (p</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;"><</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">0.05) in chicks of RIM batch. In the Heart and Plasma, chicks of RFM (3.23 and 5.05 μl/mg respectively) and RIM (5.45 and 5.35 μl/mg respectively) diets registered elevated rate of CAT. In view of these results, investigations remain to be carried out on the impact of the inclusion of </span><span><span style="font-family:Verdana;">meal and protein isolate of </span><i><span style="font-family:Verdana;">M. </span></i></span><span style="font-family:Verdana;"><i></i></span><i><i><span style="font-family:Verdana;">pruriens</span></i><span style="font-family:Verdana;"></span></i></span><span style="font-family:Verdana;"> seeds in broiler’s diet during the</span><span style="font-family:Verdana;"> growth-</span><span style="font-family:Verdana;">finishing phase</span><span style="font-family:Verdana;">.</span>
基金supported by the National Natural Science Foundation of China (31402307)the construction of public service platform for the evaluation of the value of aquatic feed and feed additives in Guangdong Province(2015A040404033)
文摘This experiment was conducted to investigate the effects of dietary arginine levels on growth performance, body composition, serum biochemical indices and resistance ability against ammonia-nitrogen stress in juvenile yellow catfish(Pelteobagrus fulvidraco). Five isonitrogenous and isolipidic diets(42%protein and 9% lipid) were formulated to contain graded levels of arginine(2.44%, 2.64%, 2.81%, 3.01% and3.23% of diet), by supplementing L-Arginine HCI. Seven hundred juvenile yellow catfish with an initial average body weight of 1.13 ± 0.02 g were randomly divided into 5 groups with 4 replicates of 35 fish each and each group was fed one of the diets. After 56 d feeding, fish were exposed to 100 mg/L of ammonia-nitrogen for 72 h. The results showed that weight gain(WG) and specific growth rate(SGR) in2.64% and 2.81% groups were significantly higher than those in 3.23% group(P < 0.05). The feed conversation ratio(FCR) in 2,64%, 2,81% and 3.01% groups was significantly decreased when compared with3,23% group. The protein efficiency ratio(PER) in 2.64% group was significantly higher than that in 2.44%and 3.23% groups(P < 0.05). The condition factor(CF) of fish was significantly higher in 2.81% group than that in 2,44% group(P < 0.05). Dietary arginine levels had no significant effect on hepatosomatic index(HSI), viscerosomatic index(VSI), and whole-body dry matter, crude protein, crude lipid, ash contents, as well as serum total protein(TP), triglyceride(TG), glucose(GLU), urea nitrogen(UN) contents and aspartate aminotransferase(AST), alanine aminotransferase(ALT) activities(P> 0.05). After the fish were challenged to ammonia-nitrogen for 72 h, their cumulative mortality rate in 2.81% group was significantly lower than that in 2.44% group(P < 0.05). The results suggested that dietary arginine level at 2.81%could optimize anti-ammonia-nitrogen stress ability of juvenile yellow catfish and a level of 3.23%arginine seemed to depress the growth performance of fish and decreased their tolerance to the ammonia-nitrogen stress under current study. A quadratic regression analysis based on WG indicated that the optimal dietary arginine requirement of juvenile yellow catfish was estimated to be 2.74% of the diet(6.45% of dietary protein) under current culture conditions.
基金Supported by the Government Subsidizes Special Funds for Outstanding Health Talents,No.0700000042.
文摘BACKGROUND According to the indexes of serum and anal function,acupuncture therapy was applied to patients with low rectal cancer in order to avoid the occurrence of anal incontinence and reduce complications.AIM To explore the clinical application and evaluate the effect of acupuncture therapy for anal function rehabilitation after low-tension rectal cancer surgery.METHODS From the anorectal surgery cases,we selected 120 patients who underwent colorectal cancer surgery between January 2020 and December 2022 and randomly divided them into a control group(n=60),observation group(n=60),and control group after surgery for lifestyle intervention(including smoking cessation and exercise),dietary factor adjustment,anal movement,and oral loperamide treatment.The serum levels of motilin,5-hydroxytryptamine,and vasoactive intestinal peptide(VIP),Wexner score for anal incontinence,and incidence of complications were compared between groups.RESULTS After treatment,the VIP and 5-hydroxytryptamine levels in the observation group were lower than those in the control group(P<0.05).The motilin level was higher than that in the control group(P<0.05).Postoperative anal incontinence was better in the observation group than in the control group(P<0.05).The incidence of complications in the observation group was 6.67%,which was significantly lower than that in the control group(21.67%;P<0.05).CONCLUSION Acupuncture therapy has a positive effect on the rehabilitation of anal function after low-tension rectal cancer surgery;it can effectively help to improve the serum indices of patients,avoid the occurrence of anal incontinence,and reduce the incidence of complications.Popularizing and applying it will be valuable.