Context: The increase of serum lipase is established for pancreatic and bile duct disorders. However, the production of this enzyme by other organs, including the lungs, leads to the question of its potential role in ...Context: The increase of serum lipase is established for pancreatic and bile duct disorders. However, the production of this enzyme by other organs, including the lungs, leads to the question of its potential role in the diagnosis of other conditions including lung diseases. Objective: The aim of the present study was to describe the profile of serum lipase in patients who suffered from the pulmonary tuberculosis and to identify its determinants. Patients and methods: A cross-sectional study was performed from July to October 2013 in four hospitals of Kinshasa (The University Hospital of Kinshasa, Lisanga medical Center and 2 medical centers of Save Army). Patients who suffered from tuberculosis were included. The levels of serum lipase, triglyceridemia, cholesterolemia, c-reactive protein were noted, as so as the hemogram profile and the prescribed treatment (category and phase). Results: One hundred and twenty-eight patients suffering from pulmonary tuberculosis were included. Forty three women (33.6%) and 85 men (66.4%). The sex ratio M/F was 1:9. Hyperlipasemia was observed in 44.5% of patients. The average level of serum lipase was 36.6 ± 5.0 IU/L (normal value: ≤ 38 IU/L) in tuberculosis patients and 30 ± 2.3 IU/L in controls The lipid profile of the patients was normal. Conclusion: L Hyperlipasemia can be encountered, in varying proportions, during pulmonary tuberculosis. It would be an indication of inflammation of the pulmonary parenchyma.展开更多
Strain of Pseudomonas Lip35 producing lipase was isolated in a refrigerator. Lipase production and characterization of this strain were investigated under different conditions. The Pseudomonas was cultivated in shakin...Strain of Pseudomonas Lip35 producing lipase was isolated in a refrigerator. Lipase production and characterization of this strain were investigated under different conditions. The Pseudomonas was cultivated in shaking flasks in a fermentation medium in various nutritional and physical environments. Lipase production has been influenced by the presence of yeast-extract, soybean powder, NaCI, and Tween-80. Maximum lipase productivity was obtained when the physical environment of the fermentation medium was optimal for 67 h. The production of lipase reached 58.9 U·mL^-1. The lipase of Pseudomonas Lip35 can be considered to be inducible, but the inducer had little influence on the production of lipase. The lipase was characterized and showed high lipolytic activity from pH 7.5-8.0. The optimum temperature was observed at 20℃ and the thermal inactivation of lipase was obvious at 60℃. The lipase activity was inhibited by K＋, stimulated by Ca^2＋, and thermostability decreased in the presence of Ca^2＋, therefore the lipase was Ca^2＋ -dependent cold-adapted enzyme.展开更多
An environment friendly bio-surfactant of rhamnolipid(RL) was used as a solvent. The enzymatic reaction of oleic acid catalyzed by lipase and lignin peroxidase(lip) was evaluated. The optimum conditions of enzymatic r...An environment friendly bio-surfactant of rhamnolipid(RL) was used as a solvent. The enzymatic reaction of oleic acid catalyzed by lipase and lignin peroxidase(lip) was evaluated. The optimum conditions of enzymatic reaction catalyzed by lipase(lip) were water to amphiphile molar ratio of 30(20), RL of 60(60) critical micelle concentration(CMC), pH of 7.0(3.0) and temperature of 40(30) °C, respectively. The change of enzyme conformation indicates that, for catalytic of lipase, water content is the most important factor of the enzymatic reaction of oleic acid, and p H for lip. With individual optimum conditions, the enzymatic efficiency of oleic acid catalyzed by lipase is higher than that by lip. In the presence of ethanol, the enzymatic reaction of oleic acid catalyzed by lipase suits Ping-Pong Bi-Bi mechanism. As an alternative to chemical reversed micelles, the RL reversed micelles are promising methods to enzymatic reaction of oleic acid.展开更多
文摘Context: The increase of serum lipase is established for pancreatic and bile duct disorders. However, the production of this enzyme by other organs, including the lungs, leads to the question of its potential role in the diagnosis of other conditions including lung diseases. Objective: The aim of the present study was to describe the profile of serum lipase in patients who suffered from the pulmonary tuberculosis and to identify its determinants. Patients and methods: A cross-sectional study was performed from July to October 2013 in four hospitals of Kinshasa (The University Hospital of Kinshasa, Lisanga medical Center and 2 medical centers of Save Army). Patients who suffered from tuberculosis were included. The levels of serum lipase, triglyceridemia, cholesterolemia, c-reactive protein were noted, as so as the hemogram profile and the prescribed treatment (category and phase). Results: One hundred and twenty-eight patients suffering from pulmonary tuberculosis were included. Forty three women (33.6%) and 85 men (66.4%). The sex ratio M/F was 1:9. Hyperlipasemia was observed in 44.5% of patients. The average level of serum lipase was 36.6 ± 5.0 IU/L (normal value: ≤ 38 IU/L) in tuberculosis patients and 30 ± 2.3 IU/L in controls The lipid profile of the patients was normal. Conclusion: L Hyperlipasemia can be encountered, in varying proportions, during pulmonary tuberculosis. It would be an indication of inflammation of the pulmonary parenchyma.
基金supported by the Major Program of the Hebei Province Commission of Science and Technology during the 11 th Five-Year-Plan period,China(06220106D)
文摘Strain of Pseudomonas Lip35 producing lipase was isolated in a refrigerator. Lipase production and characterization of this strain were investigated under different conditions. The Pseudomonas was cultivated in shaking flasks in a fermentation medium in various nutritional and physical environments. Lipase production has been influenced by the presence of yeast-extract, soybean powder, NaCI, and Tween-80. Maximum lipase productivity was obtained when the physical environment of the fermentation medium was optimal for 67 h. The production of lipase reached 58.9 U·mL^-1. The lipase of Pseudomonas Lip35 can be considered to be inducible, but the inducer had little influence on the production of lipase. The lipase was characterized and showed high lipolytic activity from pH 7.5-8.0. The optimum temperature was observed at 20℃ and the thermal inactivation of lipase was obvious at 60℃. The lipase activity was inhibited by K＋, stimulated by Ca^2＋, and thermostability decreased in the presence of Ca^2＋, therefore the lipase was Ca^2＋ -dependent cold-adapted enzyme.
基金Projects(50978087,51009063,50978088)supported by the National Natural Science Foundation of China
文摘An environment friendly bio-surfactant of rhamnolipid(RL) was used as a solvent. The enzymatic reaction of oleic acid catalyzed by lipase and lignin peroxidase(lip) was evaluated. The optimum conditions of enzymatic reaction catalyzed by lipase(lip) were water to amphiphile molar ratio of 30(20), RL of 60(60) critical micelle concentration(CMC), pH of 7.0(3.0) and temperature of 40(30) °C, respectively. The change of enzyme conformation indicates that, for catalytic of lipase, water content is the most important factor of the enzymatic reaction of oleic acid, and p H for lip. With individual optimum conditions, the enzymatic efficiency of oleic acid catalyzed by lipase is higher than that by lip. In the presence of ethanol, the enzymatic reaction of oleic acid catalyzed by lipase suits Ping-Pong Bi-Bi mechanism. As an alternative to chemical reversed micelles, the RL reversed micelles are promising methods to enzymatic reaction of oleic acid.
