Objective: To study the effect of treatment of renal anemia by combination of Bushen Jianpi Recipe (补贤健脾方, BJR, a Chinese experienced recipe for supplementing Shen and supporting Pi) and low dosage erythropoietin...Objective: To study the effect of treatment of renal anemia by combination of Bushen Jianpi Recipe (补贤健脾方, BJR, a Chinese experienced recipe for supplementing Shen and supporting Pi) and low dosage erythropoietin (EPO), and the influence of treatment on change of serum tumor necrosis factor α (TNF-α) so as to explore the possible mechanism of integrative Chinese and Western medicine (ICWM) in treating renal anemia. Methods: Patients with renal anemia were randomly divided into two groups, the ICWM group and the control group, supporting treatment such as dialysis, supplementing of ferrous, foliac acid and vitamin B12, was given to both groups. Additionally, to the ICWM group, 50 IU/kg of EPO subcutaneous injection for twice every week, and oral intake of BJR, one dose per day taken in two parts, were given, and to the control group, EPO alone, 50IU/kg by subcutaneous injecting, 3 times perweek, was given. The therapeutic course for two groups was 3 months. Blood levels of hemoglobin (Hb), hematocrit (Hct), TNF-α were measured before treatment and the therapeutic effect was observed. Results: After treatment, the levels of Hb and Hct increased significantly in both groups (P<0. 01) , comparison between the two groups in Hb and Hct after treatment for 3 months showed significant difference (P<0. 05). The serum level of TNF-α was markedly higher than normal range in both groups before treatment, it significantly lowered after treatment in the ICWM group (P< 0.05), but unchanged in the control group. Conclusion: Combination of BJR and EPO could significantly inhibit the production of TNF-α, this may be an important factor for ICWM in effectively improving sensitivity to EPO.Original article on CJITWM (Chin) 2004;24 (2): 106展开更多
目的:急性肾损伤是临床常见危重症之一,存活患者的肾脏远期预后不容乐观。文章系统评价细胞外囊泡对急性肾损伤动物模型的有效性,希望为临床试验提供启发。方法:检索PubMed、Embase、Web of Science、Cochrane Library、万方、维普、中...目的:急性肾损伤是临床常见危重症之一,存活患者的肾脏远期预后不容乐观。文章系统评价细胞外囊泡对急性肾损伤动物模型的有效性,希望为临床试验提供启发。方法:检索PubMed、Embase、Web of Science、Cochrane Library、万方、维普、中国知网和中国生物医学文献数据库中有关细胞外囊泡治疗急性肾损伤临床前研究的相关文章,检索时间为各数据库建立至2022-08-01,由2名研究人员独立提取数据,对文献采用SYRCLE动物实验偏倚风险评估表进行质量评价。采用RevMan 5.4和Stata 14.0软件对血肌酐、尿素氮、肿瘤坏死因子α、肾小管管状坏死与肾小管管型等结局指标进行Meta分析。结果:(1)共纳入19篇随机对照动物实验,包括399只啮齿动物,其中细胞外囊泡治疗组啮齿动物169只,对照组啮齿动物168只,干细胞治疗组啮齿动物62只。(2)Meta分析结果显示:与动物模型对照组相比,细胞外囊泡组血肌酐水平(SMD=-5.41,95%CI:-6.95至-3.88,P<0.001)、尿素氮水平(SMD=-4.57,95%CI:-5.94至-3.20,P<0.001)、肿瘤坏死因子α水平(SMD=-2.15,95%CI:-2.63至-1.68,P<0.001)明显降低,肾小管管状坏死(SMD=-6.15,95%CI:-9.66至-2.64,P=0.001)与肾小管管型(SMD=-3.33,95%CI:-4.17至-2.49,P=0.001)明显减少;细胞外囊泡组与间充质干细胞治疗组在改善血肌酐(SMD=-1.23,95%CI:-2.80-0.35,P=0.127)及尿素氮(SMD=-0.34,95%CI:-2.19-1.50,P=0.715)水平方面无显著差异。结论:细胞外囊泡有助于改善急性肾损伤动物模型的血肌酐和尿素氮水平,可显著降低肿瘤坏死因子α水平,并且减少肾小管管状坏死与肾小管管型,这些基础研究证据为下一步开展临床试验提供重要参考。展开更多
文摘Objective: To study the effect of treatment of renal anemia by combination of Bushen Jianpi Recipe (补贤健脾方, BJR, a Chinese experienced recipe for supplementing Shen and supporting Pi) and low dosage erythropoietin (EPO), and the influence of treatment on change of serum tumor necrosis factor α (TNF-α) so as to explore the possible mechanism of integrative Chinese and Western medicine (ICWM) in treating renal anemia. Methods: Patients with renal anemia were randomly divided into two groups, the ICWM group and the control group, supporting treatment such as dialysis, supplementing of ferrous, foliac acid and vitamin B12, was given to both groups. Additionally, to the ICWM group, 50 IU/kg of EPO subcutaneous injection for twice every week, and oral intake of BJR, one dose per day taken in two parts, were given, and to the control group, EPO alone, 50IU/kg by subcutaneous injecting, 3 times perweek, was given. The therapeutic course for two groups was 3 months. Blood levels of hemoglobin (Hb), hematocrit (Hct), TNF-α were measured before treatment and the therapeutic effect was observed. Results: After treatment, the levels of Hb and Hct increased significantly in both groups (P<0. 01) , comparison between the two groups in Hb and Hct after treatment for 3 months showed significant difference (P<0. 05). The serum level of TNF-α was markedly higher than normal range in both groups before treatment, it significantly lowered after treatment in the ICWM group (P< 0.05), but unchanged in the control group. Conclusion: Combination of BJR and EPO could significantly inhibit the production of TNF-α, this may be an important factor for ICWM in effectively improving sensitivity to EPO.Original article on CJITWM (Chin) 2004;24 (2): 106
