Crosstalk between degradation and bioenergetics: how autophagy and endolysosomal processes regulate energy production

Cells undergo metabolic reprogramming to adapt to changes in nutrient availability, cellular activity, and transitions in cell states. The balance between glycolysis and mitochondrial respiration is crucial for energy production, and metabolic reprogramming stipulates a shift in such balance to optimize both bioenergetic efficiency and anabolic requirements. Failure in switching bioenergetic dependence can lead to maladaptation and pathogenesis. While cellular degradation is known to recycle precursor molecules for anabolism, its potential role in regulating energy production remains less explored. The bioenergetic switch between glycolysis and mitochondrial respiration involves transcription factors and organelle homeostasis, which are both regulated by the cellular degradation pathways. A growing body of studies has demonstrated that both stem cells and differentiated cells exhibit bioenergetic switch upon perturbations of autophagic activity or endolysosomal processes. Here, we highlighted the current understanding of the interplay between degradation processes, specifically autophagy and endolysosomes, transcription factors, endolysosomal signaling, and mitochondrial homeostasis in shaping cellular bioenergetics. This review aims to summarize the relationship between degradation processes and bioenergetics, providing a foundation for future research to unveil deeper mechanistic insights into bioenergetic regulation.

Coagulation indices and fibrinogen degradation products as predictive biomarkers for tumor-node-metastasis staging and metastasis in gastric cancer

BACKGROUND Gastric cancer(GC)is a prevalent malignancy with a substantial health burden and high mortality rate,despite advances in prevention,early detection,and treatment.Compared with the global average,Asia,notably China,reports disproportionately high GC incidences.The disease often progresses asymptoma-tically in the early stages,leading to delayed diagnosis and compromised out-comes.Thus,it is crucial to identify early diagnostic biomarkers and enhance treatment strategies to improve patient outcomes and reduce mortality.METHODS Retrospectively analyzed the clinical data of 148 patients with GC treated at the Civil Aviation Shanghai Hospital between December 2022 and December 2023.The associations of coagulation indices-partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),fibrinogen,fibrinogen degradation products(FDP),fasting blood glucose,and D-dimer(D-D)with TNM stage and distant metastasis were examined.RESULTS Prolongation of APTT,PT,and TT was significantly correlated with the GC TNM stage.Hence,abnormal coagulation system activation was closely related to disease progression.Elevated FDP and D-D were significantly associated with distant metastasis in GC(P<0.05),suggesting that increased fibrinolytic activity contributes to increased metastatic risk.CONCLUSION Our Results reveal coagulation indices,FDPs as GC biomarkers,reflecting abnormal coagulation/fibrinolysis,aiding disease progression,metastasis prediction,and helping clinicians assess thrombotic risk for early intervention and personalized treatment plans.

Scalable Ir‑Doped NiFe_(2)O_(4)/TiO_(2)Heterojunction Anode for Decentralized Saline Wastewater Treatment and H_(2)Production

Wastewater electrolysis cells(WECs)for decentralized wastewater treatment/reuse coupled with H_(2)production can reduce the carbon footprint associated with transportation of water,waste,and energy carrier.This study reports Ir-doped NiFe_(2)O_(4)(NFI,~5 at%Ir)spinel layer with TiO_(2)overlayer(NFI/TiO_(2)),as a scalable heterojunction anode for direct electrolysis of wastewater with circumneutral pH in a single-compartment cell.In dilute(0.1 M)NaCl solutions,the NFI/TiO_(2)marks superior activity and selectivity for chlorine evolution reaction,outperforming the benchmark IrO_(2).Robust operation in near-neutral pH was confirmed.Electroanalyses including operando X-ray absorption spectroscopy unveiled crucial roles of TiO_(2) which serves both as the primary site for Cl−chemisorption and a protective layer for NFI as an ohmic contact.Galvanostatic electrolysis of NH4+-laden synthetic wastewater demonstrated that NFI/TiO_(2)not only achieves quasi-stoichiometric NH_(4)^(+)-to-N_(2)conversion,but also enhances H_(2)generation efficiency with minimal competing reactions such as reduction of dissolved oxygen and reactive chlorine.The scaled-up WEC with NFI/TiO_(2)was demonstrated for electrolysis of toilet wastewater.

Shared and Service-oriented CNC Machining System for Intelligent Manufacturing Process

To improve efficiency, reduce cost, ensure quality effectively, researchers on CNC machining have focused on virtual machine tool, cloud manufacturing, wireless manufacturing. However, low level of information shared among different systems is a common disadvantage. In this paper, a machining database with data evaluation module is set up to ensure integrity and update. An online monitoring system based on internet of things and multi-sensors ＂feel＂ a variety of signal features to ＂percept＂ the state in CNC machining process. A high efficiency and green machining parameters optimization system ＂execute＂ service-oriented manufacturing, intelligent manufacturing and green manufacturing. The intelligent CNC machining system is applied in production. CNC machining database effectively shares and manages process data among different systems. The prediction accuracy of online monitoring system is up to 98.8% by acquiring acceleration and noise in real time. High efficiency and green machining parameters optimization system optimizes the original processing parameters, and the calculation indicates that optimized processing parameters not only improve production efficiency, but also reduce carbon emissions. The application proves that the shared and service-oriented CNC machining system is reliable and effective. This research presents a shared and service-oriented CNC machining system for intelligent manufacturing process.

Service-oriented weapon systems of system portfolio selection method

Weapon system portfolio selection is an important combinatorial problem that arises in various applications,such as weapons development planning and equipment procurement,which are of concern to military decision makers.However,the existing weapon system-of-systems(SoS)is tightly coupled.Because of the diversity and connectivity of mission requirements,it is difficult to describe the direct mapping relationship from the mission to the weapon system.In the latest service-oriented research,the introduction of service modules to build a service-oriented,flexible,and combinable structure is an important trend.This paper proposes a service-oriented weapon system portfolio selection method,by introducing service to serve as an intermediary to connect missions and system selection,and transferring the weapon system selection into the service portfolio selection.Specifically,the relation between the service and the task is described through the service-task mapping matrix;and the relation between the service and the weapon system is constructed through the servicesystem mapping matrix.The service collaboration network to calculate the flexibility and connectivity of each service portfolio is then established.Through multi-objective programming,the optimal service portfolios are generated,which are further decoded into weapon system portfolios.

A Service-Oriented Architecture of Cyber-Physical System and Study on Two Key Issues

As the basis of designing and implementing a cyber-physical system (CPS), architecture research is very important but still at preliminary stage. Since CPS includes physical components, time and space constraints seriously challenge architecture study. In this paper, a service-oriented architecture of CPS was presented. Further, a two-way time synchronization algorithm for CPS service composition was put forward. And a formal method, for judging if actual CPS service meets space constraints, was suggested, which was based on space-π-calculus proposed. Finally, a case study was performed and CPS business process designed by the model and the proposed methods could run well. The application of research conclusion implies that it has rationality and feasibility.

Privacy Protection Framework with Defined Policies for Service-Oriented Architecture

Service-Oriented Architecture (SOA) is a computer systems design concept which aims to achieve reusability and integration in a distributed environment through the use of autonomous, loosely coupled, interoperable abstractions known as services. In order to interoperate, communication between services is very important due to their autonomous nature. This communication provides services with their functional strengths, but also creates the opportunity for the loss of privacy. In this paper, a Privacy Protection Framework for Service-Oriented Architecture (PPFSOA) is described. In this framework, a Privacy Service (PS) is used in combination with privacy policies to create privacy contracts that outline what can and cannot be done with a consumer’s personally identifiable information (PII). The privacy policy consists of one-to-many privacy rules, with each rule created from a set of six privacy elements: collector, what, purpose, retention, recipient and trust. The PS acts as an intermediary between the service consumer and service provider, to establish an unbiased contract before the two parties begin sending PII. It is shown how many Privacy Services work together to form the privacy protection framework. An examination of what current approaches to protecting privacy in an SOA environment is also presented. Finally, the operations the PS must perform in order to fulfill its tasks are outlined.

An agent-based service-oriented integration architecture for chemical process automation

In reality, traditional process control system built upon centralized and hierarchical structures presents a weak response to change and is easy to shut down by single failure. Aiming at these problems, a new agent-based service-oriented integration architecture was proposed for chemical process automation system. Web services were dynamically orchestrated on the internet and agent behaviors were built in them. Data analysis, model, optimization, control, fault diagnosis and so on were capsuled into different web services. Agents were used for service compositions by negotiation. A prototype system of poly(ethylene terephthalate) process automation was used as the case study to demonstrate the validation of the integration.

A Collaborative Framework for Mould Design Based on Service-Oriented Architecture

Aiming at improving the traditional object-oriented three-layer architecture for collaborative design, a service-oriented framework for mould design is established by adding a Web service layer to decoupling the direct linking of user interface to functional modules,making the system being platform independent and programming language independent,greatly facilitating the reuse of existing software and hardware resources. .NET technology is adopted to implement the framework. An example scenario is introduced to demonstrate how the system works.

Describing Service-Oriented Architecture by Extended Darwin

Describing Service-Oriented Architecture （SOA） is critical in the development of Web based system, in this paper, an approach for describing SOA by extended Darwin is proposed. The requirements for describing SOA, which are different from that of ordinary architecture, are highlighted firstly, and then a solution for extending Darwin is presented. Using the extended Darwin, service components and connectors can be described explicit by the extended construct, as well as precise operational semantics of SOA by the π-calculus. Finally an example of supply-chain management system is given for manifesting the effect of the extended Darwin.

Mechanical Modeling and Analysis of Stability Deterioration of Production Well During Marine Hydrate Depressurization Production

Different from oil and gas production,hydrate reservoirs are shallow and unconsolidated,whose mechanical properties deteriorate with hydrate decomposition.Therefore,the formations will undergo significant subsidence during depressurization,which will destroy the original force state of the production well.However,existing research on the stability of oil and gas production wells assumes the formation to be stable,and lacks consideration of the force exerted on the hydrate production well by formation subsidence caused by hydrate decomposition during production.To fill this gap,this paper proposes an analytical method for the dynamic evolution of the stability of hydrate production well considering the effects of hydrate decomposition.Based on the mechanical model of the production well,the basis for stability analysis has been proposed.A multi-field coupling model of the force state of the production well considering the effect of hydrate decomposition and formation subsidence is established,and a solver is developed.The analytical approach is verified by its good agreement with the results from the numerical method.A case study found that the decomposition of hydrate will increase the pulling-down force and reduce the supporting force,which is the main reason for the stability deterioration.The higher the initial hydrate saturation,the larger the reservoir thickness,and the lower the production pressure,the worse the stability or even instability.This work can provide a theoretical reference for the stability maintaining of the production well.

Rapid Service-Oriented Business Process Generation Method Based on Ontology

Service-oriented business process generation is a key activity in the ServiceOriented Architecture(SOA)business lifecycle,and most of the other activities such as application execution depend on the business process being developed.After the business requirements are acquired,a developer has to use specific programming technologies to orchestrate web services to generate a deployable business process.It is time-consuming to specify all the business processes from lowlevel web services,especially for an enterprise that focuses on a series of similar businesses.This paper proposes a rapid service-oriented business process generation method with domain-specific assets specified in ontology systems.Assets with different levels of granularity are reused to refine the high level business process framework for executable business processes using the Business Process Executional Language(BPEL).The new methodology significantly simplifies service-oriented business process generation by reusing assets to construct business processes.A business process generation tool is also implemented to support the efficient visual design of SOA processes with the proposed method.This paper verifies the proposed method using a shipment tracking case.The studies show that the number of reusable assets increases significantly as these projects progress,and the business process generation speed also increases at the same time.

Classification and technical target of water electrolysis for hydrogen production

Continuous efforts are underway to reduce carbon emissions worldwide in response to global climate change.Water electrolysis technology,in conjunction with renewable energy,is considered the most feasible hydrogen production technology based on the viable possibility of large-scale hydrogen production and the zero-carbon-emission nature of the process.However,for hydrogen produced via water electrolysis systems to be utilized in various fields in practice,the unit cost of hydrogen production must be reduced to$1/kg H_(2).To achieve this unit cost,technical targets for water electrolysis have been suggested regarding components in the system.In this paper,the types of water electrolysis systems and the limitations of water electrolysis system components are explained.We suggest guideline with recent trend for achieving this technical target and insights for the potential utilization of water electrolysis technology.

Study on Construction of Service-oriented Government at Grass-roots Level and Development of Farmers' Professional Cooperatives

Features of farmers' professional cooperatives in China are introduced.The establishment of these organizations is spontaneous.Their operation shows self-government.And their targets are highly pertinent.Then,I analyze the existing problems in farmers' professional cooperatives and vacancy of government functions.These cooperatives are single in content,narrow in service field,not scientific in management mode,and low in efficiency.In addition,government policy support is not put in place for these cooperatives.In view of these situations,firstly,it is proposed to speed up construction of service-oriented government at grass-roots level and promote development of farmers' professional cooperatives.Secondly,it is suggested to set up awareness of service-oriented government and enhance guidance and service for farmers' professional cooperatives.Thirdly,we should increase financial support and guide these organizations to achieve a benign development.Fourthly,we should strengthen propaganda to raise popularity and influence power of these organizations.Fifthly,it is recommended to make clear the guidance and leading functions of regional supply and marketing cooperatives to raise service efficiency.Finally,we should establish scientific evaluation and incentive system to push forward healthy development of farmers' professional cooperatives.

Upcycling plastic wastes into value-added products via electrocatalysis and photoelectrocatalysis

Plastic,renowned for its versatility,durability,and cost-effectiveness,is indispensable in modern society.Nevertheless,the annual production of nearly 400 million tons of plastic,coupled with a recycling rate of only 9%,has led to a monumental environmental crisis.Plastic recycling has emerged as a vital response to this crisis,offering sustainable solutions to mitigate its environmental impact.Among these recycling efforts,plastic upcycling has garnered attention,which elevates discarded plastics into higher-value products.Here,electrocatalytic and photoelectrocatalytic treatments stand at the forefront of advanced plastic upcycling.Electrocatalytic or photoelectrocatalytic treatments involve chemical reactions that facilitate electron transfer through the electrode/electrolyte interface,driven by electrical or solar energy,respectively.These methods enable precise control of chemical reactions,harnessing potential,current density,or light to yield valuable chemical products.This review explores recent progress in plastic upcycling through electrocatalytic and photoelectrocatalytic pathways,offering promising solutions to the plastic waste crisis and advancing sustainability in the plastics industry.

Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigs

Background Chinese indigenous pigs are popular with consumers for their juiciness,flavour and meat quality,but they have lower meat production.Insulin-like growth factor 2(IGF2) is a maternally imprinted growth factor that promotes skeletal muscle growth by regulating cell proliferation and differentiation.A single nucleotide polymorphism(SNP) within intron 3 of porcine IGF2 disrupts a binding site for the repressor,zinc finger BED-type containing 6(ZBED6),leading to up-regulation of IGF2 and causing major effects on muscle growth,heart size,and backfat thickness.This favorable mutation is common in Western commercial pig populations,but absent in most Chinese indigenous pig breeds.To improve meat production of Chinese indigenous pigs,we used cytosine base editor 3(CBE3)to introduce IGF2 intron3-C3071T mutation into porcine embryonic fibroblasts(PEFs) isolated from a male Liang Guang Small Spotted pig(LGSS),and single-cell clones harboring the desired mutation were selected for somatic cell nuclear transfer(SCNT) to generate the founder line of IGF2^(T/T) pigs.Results We found the heterozygous progeny IGF2^(C/T) pigs exhibited enhanced expression of IGF2,increased lean meat by 18%-36%,enlarged loin muscle area by 3%-17%,improved intramuscular fat(IMF) content by 18%-39%,marbling score by 0.75-1,meat color score by 0.53-1.25,and reduced backfat thickness by 5%-16%.The enhanced accumulation of intramuscular fat in IGF2^(C/T) pigs was identified to be regulated by the PI3K-AKT/AMPK pathway,which activated SREBP1 to promote adipogenesis.Conclusions We demonstrated the introduction of IGF2-intron3-C3071T in Chinese LGSS can improve both meat production and quality,and first identified the regulation of IMF deposition by IGF2 through SREBP1 via the PI3KAKT/AMPK signaling pathways.Our study provides a further understanding of the biological functions of IGF2and an example for improving porcine economic traits through precise base editing.

Product quality prediction based on RBF optimized by firefly algorithm

With the development of information technology,a large number of product quality data in the entire manufacturing process is accumulated,but it is not explored and used effectively.The traditional product quality prediction models have many disadvantages,such as high complexity and low accuracy.To overcome the above problems,we propose an optimized data equalization method to pre-process dataset and design a simple but effective product quality prediction model:radial basis function model optimized by the firefly algorithm with Levy flight mechanism(RBFFALM).First,the new data equalization method is introduced to pre-process the dataset,which reduces the dimension of the data,removes redundant features,and improves the data distribution.Then the RBFFALFM is used to predict product quality.Comprehensive expe riments conducted on real-world product quality datasets validate that the new model RBFFALFM combining with the new data pre-processing method outperforms other previous me thods on predicting product quality.

Quantifying the agreement and accuracy characteristics of four satellite-based LULC products for cropland classification in China

Various land use and land cover(LULC)products have been produced over the past decade with the development of remote sensing technology.Despite the differences in LULC classification schemes,there is a lack of research on assessing the accuracy of their application to croplands in a unified framework.Thus,this study evaluated the spatial and area accuracies of cropland classification for four commonly used global LULC products(i.e.,MCD12Q1V6,GlobCover2009,FROM-GLC and GlobeLand30)based on the harmonised FAO criterion,and quantified the relationships between four factors(i.e.,slope,elevation,field size and crop system)and cropland classification agreement.The validation results indicated that MCD12Q1 and GlobeLand30 performed well in cropland classification regarding spatial consistency,with overall accuracies of 94.90 and 93.52%,respectively.The FROMGLC showed the worst performance,with an overall accuracy of 83.17%.Overlaying the cropland generated by the four global LULC products,we found the proportions of complete agreement and disagreement were 15.51 and 44.72% for the cropland classification,respectively.High consistency was mainly observed in the Northeast China Plain,the Huang-Huai-Hai Plain and the northern part of the Middle-lower Yangtze Plain,China.In contrast,low consistency was detected primarily on the eastern edge of the northern and semiarid region,the Yunnan-Guizhou Plateau and southern China.Field size was the most important factor for mapping cropland.For area accuracy,compared with China Statistical Yearbook data at the provincial scale,the accuracies of different products in descending order were:GlobeLand30,FROM-GLC,MCD12Q1,and GlobCover2009.The cropland classification schemes mainly caused large area deviations among the four products,and they also resulted in the different ranks of spatial accuracy and area accuracy among the four products.Our results can provide valuable suggestions for selecting cropland products at the national or provincial scale and help cropland mapping and reconstruction,which is essential for food security and crop management,so they can also contribute to achieving the Sustainable Development Goals issued by the United Nations.

Service-oriented air combat simulation architecture

Service-oriented air combat simulation architecture is proposed.The core design goal is high agility which represents the ability to accommodate the simulation requirements change.Its main idea is to design model units as services that can communicate and interoperate with any other services at runtime.A service is autonomous and is fully defined by a description contract which contains some combination of syntactic,semantic,and behavioral information.Based on the architecture,air combat simulation system can be described as an abstract composition of description contracts.It becomes concrete at run time as services that implement the constituent description contracts are discovered and bind.The whole process is a continuous run-time activity that responds to simulation needs and the availability of services.This provides benefits of implementation transparency and minimal dependency between models.Thus,simulation system can minimize the impact of change on it and increase the overall efficiency to respond to requirements change.

Promoting grain production through high-standard farmland construction:Evidence in China

Food security is a strategic priority for a country’s economic development.In China,high-standard farmland construction(HSFC)is an important initiative to stabilize grain production and increase grain production capacity.Based on panel data from 31 sample provinces,autonomous regions,and municipalities in China from 2005–2017,this study explored the impact of HSFC on grain yield using the difference-in-differences(DID)method.The results showed that HSFC significantly increased total grain production,which is robust to various checks.HSFC increased grain yield through three potential mechanisms.First,it could increase the grain replanting index.Second,it could effectively reduce yield loss due to droughts and floods.Last,HSFC could strengthen the cultivated land by renovating the low-and medium-yielding fields.Heterogeneity analysis found that the HSFC farmland showed a significant increase in grain yield only in the main grain-producing areas and balanced areas.In addition,HSFC significantly increased the yields of rice,wheat,and maize while leading to a reduction in soybean yields.The findings suggest the government should continue to promote HSFC,improve construction standards,and strictly control the“non-agriculturalization”and“non-coordination”of farmland to increase grain production further.At the same time,market mechanisms should be used to incentivize soybean farming,improve returns and stabilize soybean yields.