Two new triterpenoids, 3 β, 15α, 21 β, 23-tetrahydroxy-12-oleanen-28-oic acid (1), 3β , 6 α , 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (2), together with seven known compounds, viz., paeonol (3), 3, 4-dihydro...Two new triterpenoids, 3 β, 15α, 21 β, 23-tetrahydroxy-12-oleanen-28-oic acid (1), 3β , 6 α , 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (2), together with seven known compounds, viz., paeonol (3), 3, 4-dihydroxybenzoic acid (4), scosoletin (5), anthraquinones chrysophanol (6), 4H-1-benzopyran-4-one, 5, 7-dihydroxy-2-methyl (7),β-sitosterol (8) and stigmasterol glucoside (9) were isolated by the chromatography of the silica gel, RP-18 and Sephadex-LH 20 from the EtOAc extract of Neonauclea sessilifolia (Roxb.) Merr. ( Rubiaceae ). Their structures were elucidated based on spectral analysis including 1D-, 2D-NMR (HMQC, HMBC), IR and EIMS. Among them, compound 6 was shown to possess inhibitory activity on the growth of Mycobacterium tuberculosis (Zopf) Lehmann et Neumann with a minimum inhibitory amount of 25μg, compounds 2 and 4 also showed weak inhibitory activities on the growteof M. tuberculosis.展开更多
A new alkaloid, named sessilistemonamine D, was isolated from the roots of Stemona sessilifolia and the structure and relative configuration were determined on the basis of NMR and MS spectrometric data analysis.
As a part of the ongoing search for new constituents of Stemona species in China, chemical investigation of Stemona sessilifolia, a plant used in the traditional Chinese medicine to treat respiratory disorders, was ca...As a part of the ongoing search for new constituents of Stemona species in China, chemical investigation of Stemona sessilifolia, a plant used in the traditional Chinese medicine to treat respiratory disorders, was carried out. To identify the chemical components rapidly, a selected sample of S. sessilifolia containing bibenzyls was tested using LC-ESIMS and analyzed further using stop-flow LC-UV-NMR, which was sensitive for the detection of the main constituents. LC microfractions were collected using the LC-UV-NMR technique and HR-EIMS off-line analysis was cartied out on the collected fractions. This chemical screening strategy allowed for the on-line identification of the main constituents of S. sessilifolia and provided information that was useful for a further peak-guided isolation procedure. Using these methods, four bibenzyls were isolated： two known compounds, 3,5-dihydroxy-4-methyl bibenzyl（1） and 3, 5-dihydroxy-2＇-methoxy-4-methyl bibenzyl （ 2 ）, and two novel compounds, 3,3＇-dihydroxy-5, 6＇-dimethoxy bibenzyl（3） and 3,5-dihydroxy-2＇, 5＇-dimethoxy bibenzyl （4）.展开更多
无柄醉鱼草(Buddleja sessilifolia)是狭域分布的极小种群野生植物(Plant Species with Extremely Small Populations,PSESP),大叶醉鱼草(B.davidii)则是广布种,二者亲缘关系较近,且同为四倍体。通过比较濒危物种与近缘广布种的遗传多样...无柄醉鱼草(Buddleja sessilifolia)是狭域分布的极小种群野生植物(Plant Species with Extremely Small Populations,PSESP),大叶醉鱼草(B.davidii)则是广布种,二者亲缘关系较近,且同为四倍体。通过比较濒危物种与近缘广布种的遗传多样性,可以了解濒危物种的演化历史,为制定保护策略提供理论依据。本研究通过简单重复序列(Simple Sequence Repeats,SSR)分子标记对无柄醉鱼草5个居群(126个个体)和大叶醉鱼草14个居群(206个个体)进行遗传多样性和遗传结构解析,并提出对无柄醉鱼草的保护建议。结果表明,无柄醉鱼草和大叶醉鱼草各居群的期望杂合度(He)平均值分别为0.606、0.775,Shannon′s多样性指数(I)平均值分别为1.162、1.729,两个物种均具有较高的遗传多样性,但广布种大叶醉鱼草遗传多样性水平高于狭域种无柄醉鱼草;无柄醉鱼草和大叶醉鱼草遗传分化系数(F ST)分别为0.043和0.024,两个物种居群间遗传分化程度均较低。遗传结构分析结果表明,无柄醉鱼草5个居群可划分为2个集群,居群间地理距离与遗传距离不存在相关性;大叶醉鱼草14个居群可划分为3个集群,居群间地理距离与遗传距离相关。结合野外居群现状,建议对无柄醉鱼草划分保护单元,重点对丹珠居群和独龙江隧道口居群开展就地保护,同时采取种质资源收集、人工繁育等保护措施。展开更多
Neonauclea sessilifolia belongs to Rubiaceae and its chemical constituents and pharmacological investigations were seldom reported. To search the active constituents of the plant, the ethanol of the dried stem of Neon...Neonauclea sessilifolia belongs to Rubiaceae and its chemical constituents and pharmacological investigations were seldom reported. To search the active constituents of the plant, the ethanol of the dried stem of Neonauclea sessilifolia was separated via reflux with ethanol, then the crude product was extracted with ethyl acetate and n-butanol in sequence. The ethyl acetate extract was further isolated by silica gel and Sephadex LH-20 column chromatography. A new triterpene was obtained, and the structure was identified as 3β, 7β, 21β, 23-tetrahydroxyolean-12-ene-28-oic acid via 1D, 2D NMR techniques and HR-EI-MS analysis.展开更多
文摘Two new triterpenoids, 3 β, 15α, 21 β, 23-tetrahydroxy-12-oleanen-28-oic acid (1), 3β , 6 α , 21β, 23-tetrahydroxy-12-oleanen-28-oic acid (2), together with seven known compounds, viz., paeonol (3), 3, 4-dihydroxybenzoic acid (4), scosoletin (5), anthraquinones chrysophanol (6), 4H-1-benzopyran-4-one, 5, 7-dihydroxy-2-methyl (7),β-sitosterol (8) and stigmasterol glucoside (9) were isolated by the chromatography of the silica gel, RP-18 and Sephadex-LH 20 from the EtOAc extract of Neonauclea sessilifolia (Roxb.) Merr. ( Rubiaceae ). Their structures were elucidated based on spectral analysis including 1D-, 2D-NMR (HMQC, HMBC), IR and EIMS. Among them, compound 6 was shown to possess inhibitory activity on the growth of Mycobacterium tuberculosis (Zopf) Lehmann et Neumann with a minimum inhibitory amount of 25μg, compounds 2 and 4 also showed weak inhibitory activities on the growteof M. tuberculosis.
文摘A new alkaloid, named sessilistemonamine D, was isolated from the roots of Stemona sessilifolia and the structure and relative configuration were determined on the basis of NMR and MS spectrometric data analysis.
基金Supported by Science and Technology Committee of Shanghai, P R China(No 036505003)
文摘As a part of the ongoing search for new constituents of Stemona species in China, chemical investigation of Stemona sessilifolia, a plant used in the traditional Chinese medicine to treat respiratory disorders, was carried out. To identify the chemical components rapidly, a selected sample of S. sessilifolia containing bibenzyls was tested using LC-ESIMS and analyzed further using stop-flow LC-UV-NMR, which was sensitive for the detection of the main constituents. LC microfractions were collected using the LC-UV-NMR technique and HR-EIMS off-line analysis was cartied out on the collected fractions. This chemical screening strategy allowed for the on-line identification of the main constituents of S. sessilifolia and provided information that was useful for a further peak-guided isolation procedure. Using these methods, four bibenzyls were isolated： two known compounds, 3,5-dihydroxy-4-methyl bibenzyl（1） and 3, 5-dihydroxy-2＇-methoxy-4-methyl bibenzyl （ 2 ）, and two novel compounds, 3,3＇-dihydroxy-5, 6＇-dimethoxy bibenzyl（3） and 3,5-dihydroxy-2＇, 5＇-dimethoxy bibenzyl （4）.
文摘无柄醉鱼草(Buddleja sessilifolia)是狭域分布的极小种群野生植物(Plant Species with Extremely Small Populations,PSESP),大叶醉鱼草(B.davidii)则是广布种,二者亲缘关系较近,且同为四倍体。通过比较濒危物种与近缘广布种的遗传多样性,可以了解濒危物种的演化历史,为制定保护策略提供理论依据。本研究通过简单重复序列(Simple Sequence Repeats,SSR)分子标记对无柄醉鱼草5个居群(126个个体)和大叶醉鱼草14个居群(206个个体)进行遗传多样性和遗传结构解析,并提出对无柄醉鱼草的保护建议。结果表明,无柄醉鱼草和大叶醉鱼草各居群的期望杂合度(He)平均值分别为0.606、0.775,Shannon′s多样性指数(I)平均值分别为1.162、1.729,两个物种均具有较高的遗传多样性,但广布种大叶醉鱼草遗传多样性水平高于狭域种无柄醉鱼草;无柄醉鱼草和大叶醉鱼草遗传分化系数(F ST)分别为0.043和0.024,两个物种居群间遗传分化程度均较低。遗传结构分析结果表明,无柄醉鱼草5个居群可划分为2个集群,居群间地理距离与遗传距离不存在相关性;大叶醉鱼草14个居群可划分为3个集群,居群间地理距离与遗传距离相关。结合野外居群现状,建议对无柄醉鱼草划分保护单元,重点对丹珠居群和独龙江隧道口居群开展就地保护,同时采取种质资源收集、人工繁育等保护措施。
文摘目的:研究直立百部Stemona sessilifolia(Miq.) Miq.根中的化学成分.方法:运用各种色谱方法进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构.结果:从直立百部中分离得到9个化合物,分别鉴定为Protostemotinine (1)、Stemospironine (2)、Stilbostemin B (3)、Stilbostemin D (4)、4'-Methylpinosylvin(5)、芝麻素(6)、28-羟基-正二十八烷酸-3'-甘油单酯(7)、26-羟基-正二十六烷酸-3'-甘油单酯(8)及β-谷甾醇(9).结论:化合物2～8为首次从该植物中分离得到.
文摘Neonauclea sessilifolia belongs to Rubiaceae and its chemical constituents and pharmacological investigations were seldom reported. To search the active constituents of the plant, the ethanol of the dried stem of Neonauclea sessilifolia was separated via reflux with ethanol, then the crude product was extracted with ethyl acetate and n-butanol in sequence. The ethyl acetate extract was further isolated by silica gel and Sephadex LH-20 column chromatography. A new triterpene was obtained, and the structure was identified as 3β, 7β, 21β, 23-tetrahydroxyolean-12-ene-28-oic acid via 1D, 2D NMR techniques and HR-EI-MS analysis.
