AIM To investigate how natural killer(NK) cells are affected in the elimination of hepatitis C virus(HCV) by sofosbuvir/ledipasvir, two highly effective direct-acting antivirals(DAAs). METHODS Thirteen treatment-na?ve...AIM To investigate how natural killer(NK) cells are affected in the elimination of hepatitis C virus(HCV) by sofosbuvir/ledipasvir, two highly effective direct-acting antivirals(DAAs). METHODS Thirteen treatment-na?ve and treatment-experienced chronic hepatitis C(CHC) patients were treated with sofosbuvir/ledipasvir, and NK cells were detected at baseline, weeks 2, 4, 8 and 12 during therapy, and week post of treatment(Pt)-12 and 24 after the end of therapy by multicolor flow cytometry and compared with those from 13 healthy controls. RESULTS All patients achieved sustained virological response. There was a significant decline in CD56^(bright) NK cell frequencies at week 8(P = 0.002) and week 12(P = 0.003), which were altered to the level comparable to healthy controls at week Pt-12, but no difference was observed in the frequency of CD56^(dim) NK cells. Compared with healthy controls, the expression levels of NKG2A, NKp30 and CD94 on NK cells from CHC patients at baseline were higher. NKG2A, NKp30 and CD94 started to recover at week 12 and reached the levels similar to those of healthy controls at week Pt-12 or Pt-24. Before treatment, patients have higher interferon(IFN)-γ and perforin levels than healthy controls, and IFN-γ started to recover at week 8 and reached the normalized level at week Pt-12. CONCLUSION NK cells of CHC patients can be affected by DAAs, and phenotypes and function of NK cells recover not at early stage but mainly after the end of sofosbuvir/ledipasvir treatment.展开更多
It’s well known that the cancer cell has tendency to grow fast. Chemotherapy drugs have been used in order to kill cancer growing cells and take immune system weakly. However, side effect can damage these healthy cel...It’s well known that the cancer cell has tendency to grow fast. Chemotherapy drugs have been used in order to kill cancer growing cells and take immune system weakly. However, side effect can damage these healthy cells. Moreover, it is not natural treatment. Natural alternative cancer treatments may be able to help and open new way for cancer treatment. In this work, we transfer cancer nodule to wound and we treat the nodule as wound, using Nanoskin<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span> advance cell therapy (ACT), natural extra cellular matrix which releases oxygen to the cancer tissue. Our result shows that the cancer nodule becomes like chronic wound opened and then disappeared. In addition, we obtained complete healing wound.展开更多
基金Supported by the National S&T Major Projects for Infectious Diseases Control,No.2017ZX10302201-004-001
文摘AIM To investigate how natural killer(NK) cells are affected in the elimination of hepatitis C virus(HCV) by sofosbuvir/ledipasvir, two highly effective direct-acting antivirals(DAAs). METHODS Thirteen treatment-na?ve and treatment-experienced chronic hepatitis C(CHC) patients were treated with sofosbuvir/ledipasvir, and NK cells were detected at baseline, weeks 2, 4, 8 and 12 during therapy, and week post of treatment(Pt)-12 and 24 after the end of therapy by multicolor flow cytometry and compared with those from 13 healthy controls. RESULTS All patients achieved sustained virological response. There was a significant decline in CD56^(bright) NK cell frequencies at week 8(P = 0.002) and week 12(P = 0.003), which were altered to the level comparable to healthy controls at week Pt-12, but no difference was observed in the frequency of CD56^(dim) NK cells. Compared with healthy controls, the expression levels of NKG2A, NKp30 and CD94 on NK cells from CHC patients at baseline were higher. NKG2A, NKp30 and CD94 started to recover at week 12 and reached the levels similar to those of healthy controls at week Pt-12 or Pt-24. Before treatment, patients have higher interferon(IFN)-γ and perforin levels than healthy controls, and IFN-γ started to recover at week 8 and reached the normalized level at week Pt-12. CONCLUSION NK cells of CHC patients can be affected by DAAs, and phenotypes and function of NK cells recover not at early stage but mainly after the end of sofosbuvir/ledipasvir treatment.
文摘It’s well known that the cancer cell has tendency to grow fast. Chemotherapy drugs have been used in order to kill cancer growing cells and take immune system weakly. However, side effect can damage these healthy cells. Moreover, it is not natural treatment. Natural alternative cancer treatments may be able to help and open new way for cancer treatment. In this work, we transfer cancer nodule to wound and we treat the nodule as wound, using Nanoskin<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span> advance cell therapy (ACT), natural extra cellular matrix which releases oxygen to the cancer tissue. Our result shows that the cancer nodule becomes like chronic wound opened and then disappeared. In addition, we obtained complete healing wound.
