Objective:To reveal the antibacterial activity of sequentially extracted different cold organic solvent extracts of fruits,flowers and leaves of Lawsonia inermis(L against)some pathogenic bacteria.Methods:Powders of f...Objective:To reveal the antibacterial activity of sequentially extracted different cold organic solvent extracts of fruits,flowers and leaves of Lawsonia inermis(L against)some pathogenic bacteria.Methods:Powders of fruits,flowers and leaves of L inermis were continuously extracted with dichloromethane(DCM),ethyl acetate and ethanol at ambient temperature.The dried extracts were prepared into different concentrations and tested for antibacterial activity by agar well diffusion method,and also the extracts were tested to detennine the available phytochemicals.Results:Except DCM extract of flower all other test extracts revealed inhibitory effect on all tested bacteria and their inhibitory effect differed significantly(P<0.05).The highest inhibitory effect was showed by ethyl acetate extract of flower against Staphylococcus aureus(S.aureus)and Pseudomonas aeruginosa(P.aeruginosa),and ethyl acetate extract of fruit on Escherichia coli(E.coli)and Bacillus subtilis(6.subtilis).The ethyl acetate and ethanol extracts of flower,fruit and leaf expressed inhibition even at 1 mg/100μl against all test bacteria.Among the tested phytochemicals flavonoids were detected in all test extracts except DCM extract of flower.Conclusions:The study demonstrated that the ethyl acetate and ethanol extracts of fruit and flower of L inermis are potentially better source of antibacterial agents compared to leaf extracts of respective solvents.展开更多
The Yenisei River, one of the largest rivers in the world, is contaminated with artificial radionuclides released by a Russian nuclear facility producing weapon-grade plutonium, which has been in operation for many ye...The Yenisei River, one of the largest rivers in the world, is contaminated with artificial radionuclides released by a Russian nuclear facility producing weapon-grade plutonium, which has been in operation for many years. Examination of Yenisei River sediment samples revealed the presence of artificial radionuclides typical of radioactive discharge from the Mining-and-Chemical- Combine (MCC) nuclear facility: isotopes of europium (152Eu, 154Eu, and 155Eu), cesium (137Cs and 134Cs), 60Co, 90Sr, and transuranium elements. The MCC is also a source of radioactive particles in the Yenisei. New data on radionuclide concentrations in sediments and radioactive particles in the Yenisei River were obtained using a wide range of analytical methods. Sequential extraction performed on samples of sediment cores showed different degrees of potential environmental availability of artificial radionuclides and uranium. In a few samples, 241Am was present in the unextractable form, which may be accounted for by the presence of microparticles of the reactor fuel. These microparticles were investigated using scanning electron microscopy, and their reactor origin was confirmed.展开更多
Four different methods,namely mineralogical analysis,three-stage BCR sequential extraction procedure,dynamic leaching test and Hakanson Potential Ecological Risk Index Method were used to access the environmental acti...Four different methods,namely mineralogical analysis,three-stage BCR sequential extraction procedure,dynamic leaching test and Hakanson Potential Ecological Risk Index Method were used to access the environmental activity and potential ecological risks of heavy metals in zinc leaching residue.The results demonstrate that the environmental activity of heavy metals declines in the following order：CdZnCuAsPb.Potential ecological risk indices for single heavy metal are CdZnCuAsPb.Cd has serious potential ecological risk to the ecological environment and contributes most to the potential toxicity response indices for various heavy metals in the residue.展开更多
Sequential extraction method was used to analyze and distinguish various chemical bound forms of copper and zine in rape and wheat.The results show that in these two crops,copper was mainly in the form of wate soluble...Sequential extraction method was used to analyze and distinguish various chemical bound forms of copper and zine in rape and wheat.The results show that in these two crops,copper was mainly in the form of wate soluble and ethanol soluble, which can be easily transferred in crops.The total content of various chemical bound forms of copper was higher in aboveground part than in underground part,and their content was decreased in the order of water soluble form(W.S.form)>residual form (Re.form)>ethanol soluble form(Eth.S.form)>acid soluble form (A.S.form). Zinc was mainly in the form of acid soluble, which is hard to be transferred in crops.The total content of various chemical bound forms of zinc was lower in aboveground part than in roots, and their content was decreased in the order of A.S.form>Re.form>W.S.form>Eth.S.form.In comparing with copper,a large amount of zinc was enriched in seeds and pods of rape.展开更多
基金Supported by Ministry of Higher EducationSri Lanka for providing the financial assistance for the work as payment of research allowance in accordance with circular no1/2011
文摘Objective:To reveal the antibacterial activity of sequentially extracted different cold organic solvent extracts of fruits,flowers and leaves of Lawsonia inermis(L against)some pathogenic bacteria.Methods:Powders of fruits,flowers and leaves of L inermis were continuously extracted with dichloromethane(DCM),ethyl acetate and ethanol at ambient temperature.The dried extracts were prepared into different concentrations and tested for antibacterial activity by agar well diffusion method,and also the extracts were tested to detennine the available phytochemicals.Results:Except DCM extract of flower all other test extracts revealed inhibitory effect on all tested bacteria and their inhibitory effect differed significantly(P<0.05).The highest inhibitory effect was showed by ethyl acetate extract of flower against Staphylococcus aureus(S.aureus)and Pseudomonas aeruginosa(P.aeruginosa),and ethyl acetate extract of fruit on Escherichia coli(E.coli)and Bacillus subtilis(6.subtilis).The ethyl acetate and ethanol extracts of flower,fruit and leaf expressed inhibition even at 1 mg/100μl against all test bacteria.Among the tested phytochemicals flavonoids were detected in all test extracts except DCM extract of flower.Conclusions:The study demonstrated that the ethyl acetate and ethanol extracts of fruit and flower of L inermis are potentially better source of antibacterial agents compared to leaf extracts of respective solvents.
文摘The Yenisei River, one of the largest rivers in the world, is contaminated with artificial radionuclides released by a Russian nuclear facility producing weapon-grade plutonium, which has been in operation for many years. Examination of Yenisei River sediment samples revealed the presence of artificial radionuclides typical of radioactive discharge from the Mining-and-Chemical- Combine (MCC) nuclear facility: isotopes of europium (152Eu, 154Eu, and 155Eu), cesium (137Cs and 134Cs), 60Co, 90Sr, and transuranium elements. The MCC is also a source of radioactive particles in the Yenisei. New data on radionuclide concentrations in sediments and radioactive particles in the Yenisei River were obtained using a wide range of analytical methods. Sequential extraction performed on samples of sediment cores showed different degrees of potential environmental availability of artificial radionuclides and uranium. In a few samples, 241Am was present in the unextractable form, which may be accounted for by the presence of microparticles of the reactor fuel. These microparticles were investigated using scanning electron microscopy, and their reactor origin was confirmed.
基金Project(50925417) supported by the National Natural Science Funds for Distinguished Young Scholar of ChinaProject(2010AA065203) supported by the High Technology Research and Development Program of China+2 种基金Project(2010-609) Supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education,ChinaProject(ncet-10-0840) supported by Program for New Century Excellent Talents in UniversityProject(2012FJ1080) supported by Key Projects of Science and Technology of Hunan Province,China
文摘Four different methods,namely mineralogical analysis,three-stage BCR sequential extraction procedure,dynamic leaching test and Hakanson Potential Ecological Risk Index Method were used to access the environmental activity and potential ecological risks of heavy metals in zinc leaching residue.The results demonstrate that the environmental activity of heavy metals declines in the following order：CdZnCuAsPb.Potential ecological risk indices for single heavy metal are CdZnCuAsPb.Cd has serious potential ecological risk to the ecological environment and contributes most to the potential toxicity response indices for various heavy metals in the residue.
文摘Sequential extraction method was used to analyze and distinguish various chemical bound forms of copper and zine in rape and wheat.The results show that in these two crops,copper was mainly in the form of wate soluble and ethanol soluble, which can be easily transferred in crops.The total content of various chemical bound forms of copper was higher in aboveground part than in underground part,and their content was decreased in the order of water soluble form(W.S.form)>residual form (Re.form)>ethanol soluble form(Eth.S.form)>acid soluble form (A.S.form). Zinc was mainly in the form of acid soluble, which is hard to be transferred in crops.The total content of various chemical bound forms of zinc was lower in aboveground part than in roots, and their content was decreased in the order of A.S.form>Re.form>W.S.form>Eth.S.form.In comparing with copper,a large amount of zinc was enriched in seeds and pods of rape.
