Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease....Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease. Methods A reverse-transcription recombinase-aided amplification(RT-RAA) assay was developed. This assay can be completed in one closed tube at 39℃ within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type(WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay. Results The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units(pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay. Conclusion A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype.展开更多
In this paper, an optimal multi user detector in DS/CDMA communication systems based on the mean field annealing (MFA) neural network is proposed. It is shown that the NP complete problem of minimizing the objective...In this paper, an optimal multi user detector in DS/CDMA communication systems based on the mean field annealing (MFA) neural network is proposed. It is shown that the NP complete problem of minimizing the objective function of the optimal multi user detector can be translated into minimizing an MFA network energy function. Numerical results show that the proposed detector offers significant performance gain relative to the conventional detector and decorrelating detector while it can be implemented easily in analog hardware.展开更多
The main shortcomings of direct sequence spread spectrum multiple ac-cess(DS-SSMA)communication systems are the near-far effect and multiaccess in-terferences,which impair the stability,capacity and application areas ...The main shortcomings of direct sequence spread spectrum multiple ac-cess(DS-SSMA)communication systems are the near-far effect and multiaccess in-terferences,which impair the stability,capacity and application areas of the commu-nication systems.In this paper,a new kind of multiuser detector——decorrelatingdetector with decision feedback is proposed.In linear channels,this detector can e-liminate the multiaccess interferences with low complexity.Computer simulationsverify the theoretical analysis in this paper.展开更多
基金supported by the National key research and development project [2017YFC1200505]the National Science and Technology Major Project of China [2018ZX10711001,2018ZX10101-002]the Development Grant of State Key Laboratory of Infectious Disease Prevention and Control [2015SKLID505,2014SKLID103]
文摘Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease. Methods A reverse-transcription recombinase-aided amplification(RT-RAA) assay was developed. This assay can be completed in one closed tube at 39℃ within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type(WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay. Results The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units(pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay. Conclusion A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype.
文摘In this paper, an optimal multi user detector in DS/CDMA communication systems based on the mean field annealing (MFA) neural network is proposed. It is shown that the NP complete problem of minimizing the objective function of the optimal multi user detector can be translated into minimizing an MFA network energy function. Numerical results show that the proposed detector offers significant performance gain relative to the conventional detector and decorrelating detector while it can be implemented easily in analog hardware.
文摘The main shortcomings of direct sequence spread spectrum multiple ac-cess(DS-SSMA)communication systems are the near-far effect and multiaccess in-terferences,which impair the stability,capacity and application areas of the commu-nication systems.In this paper,a new kind of multiuser detector——decorrelatingdetector with decision feedback is proposed.In linear channels,this detector can e-liminate the multiaccess interferences with low complexity.Computer simulationsverify the theoretical analysis in this paper.
