Objective:To investigate the expression of cycloxygenase-2(COX-2),and vascular endothelial growth factor(VEGF)by using immunohistochemistry method and observe the changes of histological structure between the all skin...Objective:To investigate the expression of cycloxygenase-2(COX-2),and vascular endothelial growth factor(VEGF)by using immunohistochemistry method and observe the changes of histological structure between the all skin group and the xenoskin(sheepskin)group,and explore the effect of the xenoskin(sheepskin)which is a kind of covering of autogenous microskin grafting.Methods:A total of 180 Wistar healthy male rats were randomly divided into 3 groups:the autoskin group(the control group),the alloskin group and the xenoskin group(the sheepskin group),with 60 rats in each group.By using immunohistochemistry method,the expression of COX-2 and VEGF were revealed on 3,5,7,14,21 and 28 d,with 10 executed rats in each group on each time points,and the structure of histology was observed by light microscope.Results:There were obvious difference of expression of COX-2 and VEGF on wound in the autoskin group compared with other two groups(p<.01);but there were no differences between the alloskin group and xenoskin group(p>.05).Observed on light microscope,the paralleled collagen and elastic fiber appeared in the autoskin group on 14 post-transplantation days,but they appeared in the alloskin group and the xenoskin group only on 21 post-transplantation days.Conclusions:There were no differences of the expression of COX-2 and VEGF in the alloskin group and the xenoskin group,which provided clinical theoretical basis on sheepskin as a substitute for the alloskin to cover autogenous microskin.展开更多
为探究2种不同类型的多糖对羊皮明胶凝胶特性和结构的影响,利用卡拉胶与魔芋胶复配,银耳多糖作为安全性改性剂,研究羊皮明胶凝胶强度、质构、色泽、熔化温度及结构的变化。结果表明,卡拉胶与魔芋胶复配可显著提高羊皮明胶凝胶的凝胶强...为探究2种不同类型的多糖对羊皮明胶凝胶特性和结构的影响,利用卡拉胶与魔芋胶复配,银耳多糖作为安全性改性剂,研究羊皮明胶凝胶强度、质构、色泽、熔化温度及结构的变化。结果表明,卡拉胶与魔芋胶复配可显著提高羊皮明胶凝胶的凝胶强度和质构特性,在复配胶质量比为2∶1时,凝胶强度和硬度最大,分别为(625.75±5.28)g、(172.26±0.5)g。同时,熔化温度提高至30℃以上。加入银耳多糖后,增强了复合凝胶的粘连性。L^(*)值无显著变化,而a^(*)值和b^(*)值显著提高。红外光谱图表明,亲水胶体减少了明胶凝胶分子内氢键的形成。添加银耳多糖使羰基向高波数移动。加入多糖后羊皮明胶衍射峰逐渐变得平坦,三股螺旋和左螺旋的生成被破坏。扫描电镜(scanning electron microscope,SEM)结果表明添加适量多糖后,改善了复合凝胶的网络结构,使得网络结构更加均匀致密。综上所述,卡拉胶与魔芋胶复配主要改善羊皮明胶的质构特性和熔化温度,银耳多糖主要影响明胶凝胶的色泽和粘连性、回弹力。2种多糖都对羊皮明胶的特性产生了积极的影响,这可为以后羊皮明胶在食品上的应用提供理论依据和技术指导。展开更多
文摘Objective:To investigate the expression of cycloxygenase-2(COX-2),and vascular endothelial growth factor(VEGF)by using immunohistochemistry method and observe the changes of histological structure between the all skin group and the xenoskin(sheepskin)group,and explore the effect of the xenoskin(sheepskin)which is a kind of covering of autogenous microskin grafting.Methods:A total of 180 Wistar healthy male rats were randomly divided into 3 groups:the autoskin group(the control group),the alloskin group and the xenoskin group(the sheepskin group),with 60 rats in each group.By using immunohistochemistry method,the expression of COX-2 and VEGF were revealed on 3,5,7,14,21 and 28 d,with 10 executed rats in each group on each time points,and the structure of histology was observed by light microscope.Results:There were obvious difference of expression of COX-2 and VEGF on wound in the autoskin group compared with other two groups(p<.01);but there were no differences between the alloskin group and xenoskin group(p>.05).Observed on light microscope,the paralleled collagen and elastic fiber appeared in the autoskin group on 14 post-transplantation days,but they appeared in the alloskin group and the xenoskin group only on 21 post-transplantation days.Conclusions:There were no differences of the expression of COX-2 and VEGF in the alloskin group and the xenoskin group,which provided clinical theoretical basis on sheepskin as a substitute for the alloskin to cover autogenous microskin.
文摘为探究2种不同类型的多糖对羊皮明胶凝胶特性和结构的影响,利用卡拉胶与魔芋胶复配,银耳多糖作为安全性改性剂,研究羊皮明胶凝胶强度、质构、色泽、熔化温度及结构的变化。结果表明,卡拉胶与魔芋胶复配可显著提高羊皮明胶凝胶的凝胶强度和质构特性,在复配胶质量比为2∶1时,凝胶强度和硬度最大,分别为(625.75±5.28)g、(172.26±0.5)g。同时,熔化温度提高至30℃以上。加入银耳多糖后,增强了复合凝胶的粘连性。L^(*)值无显著变化,而a^(*)值和b^(*)值显著提高。红外光谱图表明,亲水胶体减少了明胶凝胶分子内氢键的形成。添加银耳多糖使羰基向高波数移动。加入多糖后羊皮明胶衍射峰逐渐变得平坦,三股螺旋和左螺旋的生成被破坏。扫描电镜(scanning electron microscope,SEM)结果表明添加适量多糖后,改善了复合凝胶的网络结构,使得网络结构更加均匀致密。综上所述,卡拉胶与魔芋胶复配主要改善羊皮明胶的质构特性和熔化温度,银耳多糖主要影响明胶凝胶的色泽和粘连性、回弹力。2种多糖都对羊皮明胶的特性产生了积极的影响,这可为以后羊皮明胶在食品上的应用提供理论依据和技术指导。
