Cereal-based foods play an important role as a source of dietary energy and nutrients in human nutrition. The use of microorganisms by humans has a long tradition. Bread making is one of the oldest arts known to man. ...Cereal-based foods play an important role as a source of dietary energy and nutrients in human nutrition. The use of microorganisms by humans has a long tradition. Bread making is one of the oldest arts known to man. Today we know that lactic acid bacteria （LAB） and yeasts are prevailing organisms in carbohydrate-rich environments. They break down carbohydrates to mainly lactic acid, ethanol and CO2. The present study is to determine the effect of Lactobacillus bulgaricus alone and as a combination with yeast （Saccharomyces cerevisiae） using yeast as a control. In this work, different percentage of Lactobacillus bulgaricus were used to investigate how sourdough affect on the bread sensory characteristics such as volume, specific loaf volume, crust, grain, color crumb, aroma, taste, texture, pH of bread and extended shelf life of bread. Also different storage times were used to know it is affected on the shelf life and sensory characteristics of bread. The results suggest that use of sourdough in bread production is useful in improving sensory properties, decreasing fungal spoilage.展开更多
本实验以传统微生物的检测方法结合16S r DNA V6~V8可变区聚合酶链式反应-变性梯度凝胶电泳指纹图谱技术(polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE)研究在10、4℃及冰温贮藏条件下,冷鲜羊肉菌相的...本实验以传统微生物的检测方法结合16S r DNA V6~V8可变区聚合酶链式反应-变性梯度凝胶电泳指纹图谱技术(polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE)研究在10、4℃及冰温贮藏条件下,冷鲜羊肉菌相的动态变化及其货架期。经微生物计数及DGGE图谱分析,10℃贮藏条件下,6 d已达到腐败临界值,其优势腐败菌为肠杆菌属、乳酸菌属等;4℃条件下,货架期为27 d,优势腐败菌有假单胞菌属、弧菌属、嗜冷菌属、不动杆菌等;冰温贮藏,其货架期为39 d,其中假单胞菌属、嗜冷菌、不动杆菌、清酒乳杆菌等成为优势腐败菌。结果表明:冰温可有效延长冷鲜羊肉的货架期。展开更多
将真空包装的新鲜金枪鱼片和三文鱼片在0、4、8、12℃贮藏,测定不同时期的菌落总数(total viable counts,TVC)、乳酸菌数和总挥发性盐基氮(total volatile basic nitrogen,TVBN),由此建立两种鱼片冷藏货架的预测模型。应用修正的Gompert...将真空包装的新鲜金枪鱼片和三文鱼片在0、4、8、12℃贮藏,测定不同时期的菌落总数(total viable counts,TVC)、乳酸菌数和总挥发性盐基氮(total volatile basic nitrogen,TVBN),由此建立两种鱼片冷藏货架的预测模型。应用修正的Gompertz方程描述乳酸菌恒定温度下的生长动态;采用Belehradek方程描述温度对最大比生长速率(μm ax)和延滞时间(L a g)的影响。结果表明:修正的G o m p e r tz方程能很好地描述乳酸菌的生长动态;Belehradek方程对乳酸菌动力学参数描述也呈良好的线性关系。同时用贮藏在6℃的两种鱼片中乳酸菌的生长实测值,对模型进行验证,表明乳酸菌在两种鱼片中的生长状况拟合良好。展开更多
文摘Cereal-based foods play an important role as a source of dietary energy and nutrients in human nutrition. The use of microorganisms by humans has a long tradition. Bread making is one of the oldest arts known to man. Today we know that lactic acid bacteria （LAB） and yeasts are prevailing organisms in carbohydrate-rich environments. They break down carbohydrates to mainly lactic acid, ethanol and CO2. The present study is to determine the effect of Lactobacillus bulgaricus alone and as a combination with yeast （Saccharomyces cerevisiae） using yeast as a control. In this work, different percentage of Lactobacillus bulgaricus were used to investigate how sourdough affect on the bread sensory characteristics such as volume, specific loaf volume, crust, grain, color crumb, aroma, taste, texture, pH of bread and extended shelf life of bread. Also different storage times were used to know it is affected on the shelf life and sensory characteristics of bread. The results suggest that use of sourdough in bread production is useful in improving sensory properties, decreasing fungal spoilage.
文摘本实验以传统微生物的检测方法结合16S r DNA V6~V8可变区聚合酶链式反应-变性梯度凝胶电泳指纹图谱技术(polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE)研究在10、4℃及冰温贮藏条件下,冷鲜羊肉菌相的动态变化及其货架期。经微生物计数及DGGE图谱分析,10℃贮藏条件下,6 d已达到腐败临界值,其优势腐败菌为肠杆菌属、乳酸菌属等;4℃条件下,货架期为27 d,优势腐败菌有假单胞菌属、弧菌属、嗜冷菌属、不动杆菌等;冰温贮藏,其货架期为39 d,其中假单胞菌属、嗜冷菌、不动杆菌、清酒乳杆菌等成为优势腐败菌。结果表明:冰温可有效延长冷鲜羊肉的货架期。
文摘将真空包装的新鲜金枪鱼片和三文鱼片在0、4、8、12℃贮藏,测定不同时期的菌落总数(total viable counts,TVC)、乳酸菌数和总挥发性盐基氮(total volatile basic nitrogen,TVBN),由此建立两种鱼片冷藏货架的预测模型。应用修正的Gompertz方程描述乳酸菌恒定温度下的生长动态;采用Belehradek方程描述温度对最大比生长速率(μm ax)和延滞时间(L a g)的影响。结果表明:修正的G o m p e r tz方程能很好地描述乳酸菌的生长动态;Belehradek方程对乳酸菌动力学参数描述也呈良好的线性关系。同时用贮藏在6℃的两种鱼片中乳酸菌的生长实测值,对模型进行验证,表明乳酸菌在两种鱼片中的生长状况拟合良好。
