Effects of oral Shilajit tablets on sexual function and sexual quality of life among reproductive-aged women: a triple-blind randomized clinical trial

Background:Shilajit is mentioned in the“Kama Sutra”as a potent enhancer of sexual desire.This study aimed to investigate the effects of oral Shilajit tablets on sexual function and sexual quality of life among women of reproductive age.Methods:Forty-eight reproductive-aged women participated in a placebo-controlled triple-blind clinical trial.The intervention group took oral Shilajit tablets(200 mg)twice daily for 60 days and the control group took the placebo.Data collection tools were Sexual Quality of Life-Female and Female Sexual Function Index.Data were collected before the intervention,30,60,and 90 days after the start of the study.Results:Forty-three women completed the study.The mean score of total sexual function in the intervention group was significantly higher than before the intervention(P<0.001).The mean score of sexual function was 28.93 after 90 days in the intervention group while it was 22.09 in the control group.This finding was observed in most domains of the sexual function index.The mean score of sexual quality of life increased after 60 days of intervention in both groups;however,the difference was not statistically significant(P=0.094).Conclusion:The study indicated that Shilajit,as a complementary therapy,may improve sexual function and most of its domains;while there was no effect on improving the quality of sexual life.

Evaluation of safety profile of black shilajit after 91 days repeated administration in rats

Objective:To evaluate the safety of shilajit by 91 days repeated administration in different dose levels in rats.Methods:In this study the albino rats were divided into four groups. Group Ⅰ received vehicle and group Ⅱ,Ⅲ and Ⅳ received 500,2500 and 5000 mg/kg of shilajit, respectively.Finally animals were sacrificed and subjected to histopathology and iron was estimated by flame atomic absorption spectroscopy and graphite furnace.Results:The result showed that there were no significant changes in iron level of treated groups when compared with control except liver(5000 mg/kg) and histological slides of all organs revealed normal except negligible changes in liver and intestine with the highest dose of shilajit.The weight of all organs was normal when compared with control.Conclusions:The result suggests that black shilajit,an Ayurvedic formulation,is safe for long term use as a dietary supplement for a number of disoniers like iron deficiency anaemia.

Protective Roles of Shilajit in Modulating Resistin,Adiponectin,and Cytokines in Rats with Non-alcoholic Fatty Liver Disease

Objective:To evaluate the effect of Shilajit,a medicine of Ayurveda,on the serum changes in cytokines and adipokines caused by non-alcoholic fatty liver disease(NAFLD).Methods:After establishing fatty liver models by feeding a high-fat diet(HFD)for 12 weeks,35 Wistar male rats were randomly divided into5 groups,including control(standard diet),Veh(HFD+vehicle),high-dose Shilajit[H-Sh,HFD+250 mg/(kg·d)Shilajit],low-dose Shilajit[L-Sh,HFD+150 mg/(kg·d)Shilajit],and pioglitazone[HFD+10 mg/(kg·d)pioglitazone]groups,7 rats in each group.After 2-week of gavage administration,serum levels of glucose,insulin,interleukin1 beta(IL-1β),IL-6,IL-10,tumor necrosis factor-alpha(TNF-α),adiponectin,and resistin were measured,and insulin resistance index(HOMA-IR)was calculated.Results:After NAFLD induction,the serum level of IL-10 significantly increased and serum IL-1β,TNF-αlevels significantly decreased by injection of both doses of Shilajit and pioglitazone(P<0.05).Increases in serum glucose level and homeostasis model of HOMA-IR were reduced by L-Sh and H-Sh treatment in NAFLD rats(P<0.05).Both doses of Shilajit increased adiponectin and decreased serum resistin levels(P<0.05).Conclusion:The probable protective role of Shilajit in NAFLD model rats may be via modulating the serum levels of IL-1β,TNF-α,IL-10,adipokine and resistin,and reducing of HOMA-IR.

Comparative Evaluation of Different Treatment for Purulent Wounds in Dogs

To compare the healing of purulent wounds with Shilajit dressing vs. Vishnevsky dressing in dogs with purulent wounds of >2 weeks of duration, eight dogs were chosen from same ages in two groups i.e., Shilajit and Vishnevsky dressing group. Dressing was done on every day basis for more than two weeks of follow up period. Main outcome of healing measure was completed at three weeks. Wound healing status was assessed at three days intervals till end of three weeks. Shilajit treated achieved complete decrease in the wound surface area, effect of healing score in Shilajit dressing group in comparison to the Vishnevsky dressing group at p > 0.05 level of significance. Shilajit is highly effective in achieving a characteristic feature of regenerative and granulation healing of purulent wounds as compared to Vishnevsky dressing group in dogs.

塔斯马依的研究进展

塔斯马依(mumie,shilajit)是夏天天气变热时从山的岩缝里流出的一种淡褐至黑色脂状物,它被哈萨克医应用于骨折及多种炎症性疾患,在印度、俄罗斯、哈萨克斯坦等国家的传统医学中应用也较广。据文献报道,塔斯马依具有抗炎、抗溃疡、抗菌、清除自由基、抗氧化、提高记忆力、抗糖尿病、抗应激、抗过敏、免疫调节、抗艾滋病、促进合成代谢、刺激机体再生过程等作用,其主要生理活性可能归因于二苯并-α-吡喃酮类化合物、腐植酸和黄腐酸。

传统药物喜来芝石的研究进展

喜来芝石作为天然存在的一种有机物,主要分布在陆上丝绸之路沿线国家的山区,作为一种适应原,以腐殖质为主要成分,具有抗氧化、抗炎、免疫调节等药理作用,主要用于治疗泌尿生殖疾病、消化功能障碍及神经性疾病等。喜来芝石相关研究仍处于初步阶段,喜来芝石的来源、成分、有效物质基础和质控都值得我们进一步深入研究。相信经过系统的整理挖掘后,喜来芝石将会成为重要的传统药物。

喜来芝石中提取的腐植酸与阿司匹林的分子络合物及其表征

阿司匹林具有退热、消炎、止痛和抗血小板凝集的作用。乙酰水杨酸分子含一个羧基和一个酯基。酯基容易被水解,导致其药效降低,并会对人体产生副作用。本研究的目的是用冻干技术和溶剂挥发技术,以原料的物质的量比为1∶1和1∶2,制备阿司匹林和腐植酸固态络合物。阿司匹林和腐植酸分子间相互作用通过差示扫描量热法(DSC)、X射线衍射(XRD)、傅里叶变换红外光谱学法(FT-IR)和扫描电子显微镜法(SEM)进行研究。用这些技术能清晰表明固态包合络合物的结构。对物质的量比为1∶2冻干络合物研究表明,阿司匹林的稳定性和溶出率显著提高。优选的冻干阿司匹林与腐植酸(1∶2)络合物对抑制鼠爪水肿和抗溃疡作用测得的最低值(0.63±0.10)与单纯的阿司匹林相比,有明显的降低,证明用其治疗具有非常显著的抗炎作用(P<0.05)。

黄腐酸和羟丙基-β-环糊精对阿司匹林分解的影响比较

研究了黄腐酸(FA)作为一种的新的络合剂,与羟丙基-β-环糊精络合物(Hp-β-CD)相比,对阿司匹林(ASA)分解的影响。ASA与FA以1:0.5、1:1、1:2(物质的量比)络合,并用冷冻干燥、溶剂蒸发和喷雾干燥三种方法进行干燥。ASA-羟丙基-β-环糊精(1:1)络合物通过喷雾干燥制备,并与最佳FA络合物进行比较。将所有的络合物和ASA放入贴好标签的铝塑小包中密封,在温度为40±2℃、相对湿度为75%±5%的稳定暗箱中存放120d。分别在0、30d、60d、90d和120d分析各样品的水杨酸含量。以物质的量比为1:1进行络合的ASA-FA喷雾干燥络合物中水杨酸含量为4.31%,该络合物在所有不同制备方法的络合物中具有最佳稳定性。然而,1:1的ASA-羟丙基-β-环糊精喷雾干燥络合物中测得水杨酸含量只有2.35%。与FA相比,ASA与羟丙基-β-环糊精络合,其稳定性更强。为提高阿司匹林的稳定性,对一种新型FA络合剂进行了研究。与1:1ASA-FA喷雾干燥络合物相比,ASA与羟丙基-β-环糊精1:1络合时,ASA的稳定性有显著改善。

喜来芝:一种具有潜在促认知活性的植物复合物

喜来芝是一种长期通过微生物分解特定植物而形成的自然物质,主要存在于喜马拉雅山地区。它是一种有效且安全的膳食补充剂,恢复能量平衡并且可以预防某些疾病。最近的研究表明,喜来芝可以控制与衰老相关的认知紊乱。其主要成分黄腐酸可以阻止牛磺酸的自动累积,因此开辟了一条治疗老年痴呆症的新方法。在本质上它是一种有益于人类健康的保健品。考虑到喜来芝在医学领域尤其是神经科学应用的影响,更多的生理和临床上的研究是必要的,以便了解喜来芝的有机分子,尤其是黄腐酸和微量元素在生物体分子和细胞水平的作用。