The utilization of metabolomics approaches to explore the metabolic mechanisms underlying plant fitness and adaptation to dynamic environments is growing,highlighting the need for an efficient and user-friendly toolki...The utilization of metabolomics approaches to explore the metabolic mechanisms underlying plant fitness and adaptation to dynamic environments is growing,highlighting the need for an efficient and user-friendly toolkit tailored for analyzing the extensive datasets generated by metabolomics studies.Current protocols for metabolome data analysis often struggle with handling large-scale datasets or require programming skills.To address this,we present Met Miner(https://github.com/Shawn Wx2019/Met Miner),a user-friendly,full-functionality pipeline specifically designed for plant metabolomics data analysis.Built on R shiny,MetMiner can be deployed on servers to utilize additional computational resources for processing large-scale datasets.Met Miner ensures transparency,traceability,and reproducibility throughout the analytical process.Its intuitive interface provides robust data interaction and graphical capabilities,enabling users without prior programming skills to engage deeply in data analysis.Additionally,we constructed and integrated a plant-specific mass spectrometry database into the Met Miner pipeline to optimize metabolite annotation.We have also developed MDAtoolkits,which include a complete set of tools for statistical analysis,metabolite classification,and enrichment analysis,to facilitate the mining of biological meaning from the datasets.Moreover,we propose an iterative weighted gene co-expression network analysis strategy for efficient biomarker metabolite screening in large-scale metabolomics data mining.In two case studies,we validated MetMiner's efficiency in data mining and robustness in metabolite annotation.Together,the Met Miner pipeline represents a promising solution for plant metabolomics analysis,providing a valuable tool for the scientific community to use with ease.展开更多
基金supported by the National Key Research and Development Program of China(No.2021YFA1300401)the Henan Key Scientific Research Programs to Universities and Colleges(No.22ZX006,to X.Z.)+1 种基金the National Natural Science Foundation of China(No.31970323 and 32170269,to X.Z.)the Postdoctoral Research Initiation Program in Henan Province(No.HN2022138 to X.W.)。
文摘The utilization of metabolomics approaches to explore the metabolic mechanisms underlying plant fitness and adaptation to dynamic environments is growing,highlighting the need for an efficient and user-friendly toolkit tailored for analyzing the extensive datasets generated by metabolomics studies.Current protocols for metabolome data analysis often struggle with handling large-scale datasets or require programming skills.To address this,we present Met Miner(https://github.com/Shawn Wx2019/Met Miner),a user-friendly,full-functionality pipeline specifically designed for plant metabolomics data analysis.Built on R shiny,MetMiner can be deployed on servers to utilize additional computational resources for processing large-scale datasets.Met Miner ensures transparency,traceability,and reproducibility throughout the analytical process.Its intuitive interface provides robust data interaction and graphical capabilities,enabling users without prior programming skills to engage deeply in data analysis.Additionally,we constructed and integrated a plant-specific mass spectrometry database into the Met Miner pipeline to optimize metabolite annotation.We have also developed MDAtoolkits,which include a complete set of tools for statistical analysis,metabolite classification,and enrichment analysis,to facilitate the mining of biological meaning from the datasets.Moreover,we propose an iterative weighted gene co-expression network analysis strategy for efficient biomarker metabolite screening in large-scale metabolomics data mining.In two case studies,we validated MetMiner's efficiency in data mining and robustness in metabolite annotation.Together,the Met Miner pipeline represents a promising solution for plant metabolomics analysis,providing a valuable tool for the scientific community to use with ease.
