Breeding program strategies to develop novel short grain white rice varieties such as japonica(short grain)that introgress biotic stress resistance and high grain quality have been developed usingindica rice(Pin Kaset...Breeding program strategies to develop novel short grain white rice varieties such as japonica(short grain)that introgress biotic stress resistance and high grain quality have been developed usingindica rice(Pin Kaset+4 and Riceberry)for applications in japonica rice(Kashihikari)improvement.Fourbreeding lines showing promising agronomic performance with short grain and low amylose content(<20%)were obtained.In addition,sensory testing of these breeding lines showed high scores thatsimilar to Kashihikari.Two promising lines,KP48-1-5and KP48-1-9,which possessed a combination offour genes resistance to different biotic stresses(Bph3+TPS+Xa21+Pi-ta)and four genes for grainquality(G83+SSlla+w+badh2),were deveiaped using marker-assisted selection(MAS)with thepedigree method.The current study clearly illustrated the successful use of MAS in combining resistanceto multiple biotic stresses while maintaining a high yield potential and preferred grain quality.Moreover,the results indicated that this breeding program,which includes crossing temperate japonica with indica,can create novel short grain rice varieties adapted to a trapical environment like the japonica type.展开更多
为研究穗发育相关基因的分子机制,对水稻“武运粳7号”辐射诱变,筛选到1个短穗小粒突变体spsg(short panicle and small grain),调查统计了该突变体的各项农艺性状,对其粒子进行扫描电镜观察,利用图位克隆技术对目的基因进行精细定位、...为研究穗发育相关基因的分子机制,对水稻“武运粳7号”辐射诱变,筛选到1个短穗小粒突变体spsg(short panicle and small grain),调查统计了该突变体的各项农艺性状,对其粒子进行扫描电镜观察,利用图位克隆技术对目的基因进行精细定位、测序鉴定及穗长相关的单倍型分析.结果表明:与野生型对照相比,突变体spsg的一级枝梗数和每穗粒数显著下降,粒长和粒宽均变小,导致穗长显著变短;该突变体的籽粒颖壳表皮细胞的长度变短;目的基因定位在水稻第11号染色体的190 kb区间内,对候选基因的测序发现,SP1(short panicle 1)基因的编码区因缺失11个碱基而发生移码突变,spsg是SP1基因的1个新等位变异;SPSG基因在籼、粳型水稻品种间存在明显的分化,籼稻品种的单倍型穗长显著优于粳稻品种.研究结果为深入解析水稻穗发育的分子机制提供了一定的理论依据.展开更多
基金supported by the Tana Group International Co.Ltd.,Thailand(2015-2019).
文摘Breeding program strategies to develop novel short grain white rice varieties such as japonica(short grain)that introgress biotic stress resistance and high grain quality have been developed usingindica rice(Pin Kaset+4 and Riceberry)for applications in japonica rice(Kashihikari)improvement.Fourbreeding lines showing promising agronomic performance with short grain and low amylose content(<20%)were obtained.In addition,sensory testing of these breeding lines showed high scores thatsimilar to Kashihikari.Two promising lines,KP48-1-5and KP48-1-9,which possessed a combination offour genes resistance to different biotic stresses(Bph3+TPS+Xa21+Pi-ta)and four genes for grainquality(G83+SSlla+w+badh2),were deveiaped using marker-assisted selection(MAS)with thepedigree method.The current study clearly illustrated the successful use of MAS in combining resistanceto multiple biotic stresses while maintaining a high yield potential and preferred grain quality.Moreover,the results indicated that this breeding program,which includes crossing temperate japonica with indica,can create novel short grain rice varieties adapted to a trapical environment like the japonica type.
文摘为研究穗发育相关基因的分子机制,对水稻“武运粳7号”辐射诱变,筛选到1个短穗小粒突变体spsg(short panicle and small grain),调查统计了该突变体的各项农艺性状,对其粒子进行扫描电镜观察,利用图位克隆技术对目的基因进行精细定位、测序鉴定及穗长相关的单倍型分析.结果表明:与野生型对照相比,突变体spsg的一级枝梗数和每穗粒数显著下降,粒长和粒宽均变小,导致穗长显著变短;该突变体的籽粒颖壳表皮细胞的长度变短;目的基因定位在水稻第11号染色体的190 kb区间内,对候选基因的测序发现,SP1(short panicle 1)基因的编码区因缺失11个碱基而发生移码突变,spsg是SP1基因的1个新等位变异;SPSG基因在籼、粳型水稻品种间存在明显的分化,籼稻品种的单倍型穗长显著优于粳稻品种.研究结果为深入解析水稻穗发育的分子机制提供了一定的理论依据.
