一次,在与一位外籍英语教师谈话时,我用了这样一个句子:“There’s no describing it.”(“简直无法形容。”)听了这话后,外籍教师称赞说这是“idiomatic English”。受到外籍教师称赞后,当然很自信,第二次不假思索又用了一个同样结构...一次,在与一位外籍英语教师谈话时,我用了这样一个句子:“There’s no describing it.”(“简直无法形容。”)听了这话后,外籍教师称赞说这是“idiomatic English”。受到外籍教师称赞后,当然很自信,第二次不假思索又用了一个同样结构的句子:展开更多
Four sampled corpora have been used for a quantitative analysis on usage of past participles(PPs) in maritime English (ME) and general English(GE). Part-of-speech tagging software CLAWS5 and AntConc 3.2.0 are used for...Four sampled corpora have been used for a quantitative analysis on usage of past participles(PPs) in maritime English (ME) and general English(GE). Part-of-speech tagging software CLAWS5 and AntConc 3.2.0 are used for extraction of PPs and da-ta collection. Standardized frequency based on 100000 words is adopted to ensure the comparability of PPs found in different corpo-ra. This study covers analysis of overall frequency distribution and different grammatical functions of PPs. Results show that ME prefers using more PPs and these PPs are more likely to be used as predicative and postpositive attribute than does GE.Detailed comparison and contrast about the use of PPs between GE and ME are also made, thus providing helpful information for learners or teachers of maritime English.展开更多
The method of immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep was established using monoclonal antibody. Genomic DNA was isolated from Chinese Short-tailed Hart sheep blood. U...The method of immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep was established using monoclonal antibody. Genomic DNA was isolated from Chinese Short-tailed Hart sheep blood. Using the polymerase chain reaction technique, PrP27-30 gene sequence was amplified from Chinese Short-tailed Han sheep genomic DNA. By recombinant DNA technology, the recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained. Then, using standard methodology of myeloma cell fusion, a panel of monoclonal antibodies was generated. With mAbs, scrapie in Chinese Short-tailed Han sheep was detected by immunohistochemistry assay. The recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained and a panel of six hybridoma cell lines secreting specific antibodies to Chinese Short-tailed Han sheep PrP27-30 related to scrapie was obtained with one fusion between myeloma Sp2/0 and spleen ceils from mice immunized with the purified recombinant protein. Four hybridoma cell lines can be used in immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep. So that the special monoclonal antibody developed in author＇s institute can be used to detect PrP^sc of scrapie in Chinese Short-tailed Han sheep by immunohistochemistry in China.展开更多
文摘Four sampled corpora have been used for a quantitative analysis on usage of past participles(PPs) in maritime English (ME) and general English(GE). Part-of-speech tagging software CLAWS5 and AntConc 3.2.0 are used for extraction of PPs and da-ta collection. Standardized frequency based on 100000 words is adopted to ensure the comparability of PPs found in different corpo-ra. This study covers analysis of overall frequency distribution and different grammatical functions of PPs. Results show that ME prefers using more PPs and these PPs are more likely to be used as predicative and postpositive attribute than does GE.Detailed comparison and contrast about the use of PPs between GE and ME are also made, thus providing helpful information for learners or teachers of maritime English.
基金the National Natural Sci-ence Foundation of China (C02030606)948 Project from Agriculture Ministry of China (2001-366)
文摘The method of immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep was established using monoclonal antibody. Genomic DNA was isolated from Chinese Short-tailed Hart sheep blood. Using the polymerase chain reaction technique, PrP27-30 gene sequence was amplified from Chinese Short-tailed Han sheep genomic DNA. By recombinant DNA technology, the recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained. Then, using standard methodology of myeloma cell fusion, a panel of monoclonal antibodies was generated. With mAbs, scrapie in Chinese Short-tailed Han sheep was detected by immunohistochemistry assay. The recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained and a panel of six hybridoma cell lines secreting specific antibodies to Chinese Short-tailed Han sheep PrP27-30 related to scrapie was obtained with one fusion between myeloma Sp2/0 and spleen ceils from mice immunized with the purified recombinant protein. Four hybridoma cell lines can be used in immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep. So that the special monoclonal antibody developed in author＇s institute can be used to detect PrP^sc of scrapie in Chinese Short-tailed Han sheep by immunohistochemistry in China.
