Histopathological effects of sublethal (0.1, 0.2, 0.3 ppm) and lethal (0.5, 1.0 ppm)solutions of mercuric chloride (HgCl2) have been studied on the gills of the air breathing catfish, Heteropneustes fossilis at the in...Histopathological effects of sublethal (0.1, 0.2, 0.3 ppm) and lethal (0.5, 1.0 ppm)solutions of mercuric chloride (HgCl2) have been studied on the gills of the air breathing catfish, Heteropneustes fossilis at the intervals ranging from 6h to 15 days. Thickening of the epithelium, swelling and hyperplasia of the mucous cells, fusion of secondary lamellae, formation of interlamellar bridge and deposition of mucous over the entire surface are some noteworthy features of mercury poisoning in sublethal concentrations. Acute pathological manifestations are formation of subepithelial space, sloughing of the epithelial layer, hemorrhage and hypertrophy of the mucous cells. Causes and impact of these cellular alterations affecting survival of the fish have been discussed展开更多
文摘Histopathological effects of sublethal (0.1, 0.2, 0.3 ppm) and lethal (0.5, 1.0 ppm)solutions of mercuric chloride (HgCl2) have been studied on the gills of the air breathing catfish, Heteropneustes fossilis at the intervals ranging from 6h to 15 days. Thickening of the epithelium, swelling and hyperplasia of the mucous cells, fusion of secondary lamellae, formation of interlamellar bridge and deposition of mucous over the entire surface are some noteworthy features of mercury poisoning in sublethal concentrations. Acute pathological manifestations are formation of subepithelial space, sloughing of the epithelial layer, hemorrhage and hypertrophy of the mucous cells. Causes and impact of these cellular alterations affecting survival of the fish have been discussed
文摘目的探讨亚甲蓝光化学灭活病毒方法对输血前试验的干扰及应用的可行性。方法 1)连续选取本院配血标本10(人)份(5 mL/份),每份标本均分为三,分别以巴氏消毒法(56℃30 min)、二磷酸氯喹和亚甲蓝光化学法做灭活病毒试验,灭活后的标本做ABO、RhD血型鉴定,观察3种方法应用后标本的溶血情况、红细胞回收率和血型鉴定结果。2)连续选取本院配血标本41份(5 mL/份),每份标本一分为二,分别入观察组和对照组,观察组做亚甲蓝光化学法灭活处理后,同未做病毒灭活处理的对照组做血型抗原强度、血型抗体效价及输血前3项检测的对比试验。结果 1)巴氏消毒法溶血情况严重,而二磷酸氯喹和亚甲蓝光化学法无明显溶血;巴氏消毒法处理后的红细胞出现凝集,无法做出ABO、RhD血型鉴定结果,另外2种方法则不受影响。2)血型抗原强度、血型抗体效价及3项输血前试验,观察组与对照组比较没有明显差异(P>0.05),抗体效价积分分别为63.68±7.57 vs 62.33±8.53,48.83±8.56 vs 50.23±9.11(P>0.05)。结论亚甲蓝光化学法灭活病毒对临床配血标本输血前试验无明显影响,可用于疫病流行期间的输血前试验以降低医学实验人员受血液标本感染的风险。